O09165 (CASQ1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calsequestrin-1

Alternative name(s):
Calsequestrin, skeletal muscle isoform

Gene names

Name:

Casq1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. The skeletal muscle CASQ1) binds around 80 Ca2+ ions, while the cardiac CASQ2) binds approximately 60 Ca2+ ions By similarity.
Subcellular location	Sarcoplasmic reticulum lumen. Note: This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells.
Tissue specificity	Skeletal muscle.
Sequence similarities	Belongs to the calsequestrin family.
Sequence caution	The sequence AAC63616.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Sarcoplasmic reticulum
Domain	Signal
Ligand	Calcium
Molecular function	Muscle protein
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endoplasmic reticulum organization Inferred from mutant phenotype PubMed 17627988. Source: MGI regulation of sequestering of calcium ion Inferred from electronic annotation. Source: Compara regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion Inferred from mutant phenotype PubMed 17627988. Source: MGI response to denervation involved in regulation of muscle adaptation Inferred from electronic annotation. Source: Compara response to heat Inferred from mutant phenotype PubMed 19237502. Source: MGI response to organic substance Inferred from electronic annotation. Source: Compara skeletal muscle tissue development Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara I band Inferred from electronic annotation. Source: Compara T-tubule Inferred from electronic annotation. Source: Compara mitochondrion Inferred from direct assay PubMed 14651853. Source: MGI sarcoplasmic reticulum lumen Inferred from direct assay PubMed 16600288. Source: MGI terminal cisterna lumen Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 405	371	Calsequestrin-1		PRO_0000412170
Regions
	Compositional bias	353 – 405	53	Asp-rich
Amino acid modifications
	Modified residue	387	1	Phosphothreonine By similarity
	Glycosylation	350	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O09165 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: 813A8DD681434E59	FASTA	405	46,378
	10 20 30 40 50 60 MRATDRMGAR AVSELRLALL FVLVLGTPRL GVQGEDGLDF PEYDGVDRVI NVNAKNYKNV 70 80 90 100 110 120 FKKYEVLALL YHEPPEDDKA SQRQFEMEEL ILELAAQVLE DKGVGFGLVD SEKDAAVAKK 130 140 150 160 170 180 LGLTEEDSVY VFKGDEVIEY DGEFSADTLV EFLLDVLEDP VELIEGEREL QAFENIEDEI 190 200 210 220 230 240 KLIGYFKSKD SEHYKAYEDA AEEFHPYIPF FATFDSKVAK KLTLKLNEID FYEAFMEEPM 250 260 270 280 290 300 TIPDKPNSEE EIVSFVEEHR RSTLRKLKPE SMYETWEDDL DGIHIVAFAE EADPDGYEFL 310 320 330 340 350 360 ETLKAVAQDN TENPDLSIIW IDPDDFPLLV PYWEKTFDID LSAPQIGVVN VTDADSIWME 370 380 390 400 MDNEEDLPSA DELEDWLEDV LEGEINTEDD DDDDDDDDDD DDDDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the genes encoding mouse cardiac and skeletal calsequestrins: expression pattern during embryogenesis." Park K.-W., Goo J.H., Chung H.-S., Kim H., Kim D.-H., Park W.-J. Gene 217:25-30(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U93291 mRNA. Translation: AAC63616.1. Different initiation. AC074310 Genomic DNA. No translation available.
IPI	IPI00986042.
RefSeq	NP_033943.2. NM_009813.2.
UniGene	Mm.12829.
3D structure databases
ProteinModelPortal	O09165.
SMR	O09165. Positions 37-388.
ModBase	Search...
PTM databases
PhosphoSite	O09165.
Proteomic databases
PaxDb	O09165.
PRIDE	O09165.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000003554; ENSMUSP00000003554; ENSMUSG00000007122.
GeneID	12372.
KEGG	mmu:12372.
UCSC	uc007dqa.2. mouse.
Organism-specific databases
CTD	844.
MGI	MGI:1309468. Casq1.
Phylogenomic databases
eggNOG	NOG77804.
GeneTree	ENSGT00390000019377.
HOGENOM	HOG000049047.
HOVERGEN	HBG050805.
InParanoid	O09165.
OMA	DTIVEFL.
OrthoDB	EOG4W0XD8.
Gene expression databases
ArrayExpress	O09165.
Bgee	O09165.
CleanEx	MM_CASQ1.
Genevestigator	O09165.
GermOnline	ENSMUSG00000007122. Mus musculus.
Family and domain databases
Gene3D	3.40.30.10. 3 hits.
InterPro	IPR001393. Calsequestrin. IPR018233. Calsequestrin_CS. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR10033. PTHR10033. 1 hit.
Pfam	PF01216. Calsequestrin. 1 hit. [Graphical view]
PRINTS	PR00312. CALSEQUESTRN.
SUPFAM	SSF52833. Thiordxn-like_fd. 3 hits.
PROSITE	PS00863. CALSEQUESTRIN_1. 1 hit. PS00864. CALSEQUESTRIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CASQ1. mouse.
NextBio	281074.
SOURCE	Search...

Entry information

Entry name

CASQ1_MOUSE

Accession

Primary (citable) accession number: O09165

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program