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Protein

Calsequestrin-1

Gene

Casq1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca2+ ions (By similarity). Regulates the release of lumenal Ca2+ via the calcium release channel RYR1; this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca2+ entry (SOCE) activity (By similarity).By similarity3 Publications

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • identical protein binding Source: UniProtKB

GO - Biological processi

  • endoplasmic reticulum organization Source: MGI
  • positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
  • positive regulation of store-operated calcium channel activity Source: UniProtKB
  • protein polymerization Source: UniProtKB
  • regulation of muscle contraction Source: MGI
  • regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: MGI
  • regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion Source: UniProtKB
  • regulation of store-operated calcium entry Source: UniProtKB
  • response to denervation involved in regulation of muscle adaptation Source: Ensembl
  • response to heat Source: MGI
  • response to organic substance Source: Ensembl
  • sarcomere organization Source: UniProtKB
  • skeletal muscle tissue development Source: Ensembl

Keywordsi

Molecular functionMuscle protein
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels
R-MMU-5578775 Ion homeostasis

Names & Taxonomyi

Protein namesi
Recommended name:
Calsequestrin-1
Alternative name(s):
Calmitine1 Publication
Calsequestrin, skeletal muscle isoform
Gene namesi
Name:Casq1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1309468 Casq1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Sarcoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile, but have a lower body weight than wild-type, due to a reduction in fast-twitch muscle mass. Fast-twitch muscle from mutant mice exhibits slower contraction kinetics and requires more time to achieve peak tension and to achieve half-relaxation after a contraction. Fast-twitch muscle fibers from mutant mice show a strikingly altered structure of the calcium release units in the sarcoplasmic reticulum with a strongly increased number of ryanodine receptors, plus narrower sarcoplasmic reticulum cisternae. In addition, the number of mitochondria is increased in mutant muscle. Mutant muscle fibers show smaller calcium transients upon electrical stimulation and release less Ca2+ in response to caffeine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 341 PublicationAdd BLAST34
ChainiPRO_000041217035 – 405Calsequestrin-1Add BLAST371

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43PhosphotyrosineBy similarity1
Modified residuei81PhosphoserineBy similarity1
Modified residuei124PhosphothreonineBy similarity1
Modified residuei216PhosphoserineBy similarity1
Glycosylationi350N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO09165
PaxDbiO09165
PeptideAtlasiO09165
PRIDEiO09165

PTM databases

iPTMnetiO09165
PhosphoSitePlusiO09165

Expressioni

Tissue specificityi

Detected in skeletal muscle (at protein level). Detected in skeletal muscle.4 Publications

Gene expression databases

BgeeiENSMUSG00000007122
CleanExiMM_CASQ1
ExpressionAtlasiO09165 baseline and differential
GenevisibleiO09165 MM

Interactioni

Subunit structurei

Monomer; increases in response to a depletion of intracellular calcium. Homodimer. homotetramer and homopolymer. Can form linear homooligomers. Ca2+ ions promote oligomerization. Interacts (via C-terminal end and preferentially with the monomeric form) with STIM1; this interaction increases in response to a depletion of intracellular calcium, decreases both STIM1 aggregation and clustering, interaction of STIM1 with ORAI1 and store-operated Ca2+ entry (SOCE) activity. Interacts with ASPH and TRDN.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003554

Structurei

3D structure databases

ProteinModelPortaliO09165
SMRiO09165
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi353 – 405Asp-richAdd BLAST53

Sequence similaritiesi

Belongs to the calsequestrin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGY5 Eukaryota
ENOG4111R2M LUCA
GeneTreeiENSGT00390000019377
HOGENOMiHOG000049047
HOVERGENiHBG050805
InParanoidiO09165
OMAiSGVQGEE
OrthoDBiEOG091G08VX
TreeFamiTF313796

Family and domain databases

InterProiView protein in InterPro
IPR001393 Calsequestrin
IPR018233 Calsequestrin_CS
IPR036249 Thioredoxin-like_sf
PANTHERiPTHR10033 PTHR10033, 1 hit
PfamiView protein in Pfam
PF01216 Calsequestrin, 1 hit
SUPFAMiSSF52833 SSF52833, 3 hits
PROSITEiView protein in PROSITE
PS00863 CALSEQUESTRIN_1, 1 hit
PS00864 CALSEQUESTRIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O09165-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRATDRMGAR AVSELRLALL FVLVLGTPRL GVQGEDGLDF PEYDGVDRVI
60 70 80 90 100
NVNAKNYKNV FKKYEVLALL YHEPPEDDKA SQRQFEMEEL ILELAAQVLE
110 120 130 140 150
DKGVGFGLVD SEKDAAVAKK LGLTEEDSVY VFKGDEVIEY DGEFSADTLV
160 170 180 190 200
EFLLDVLEDP VELIEGEREL QAFENIEDEI KLIGYFKSKD SEHYKAYEDA
210 220 230 240 250
AEEFHPYIPF FATFDSKVAK KLTLKLNEID FYEAFMEEPM TIPDKPNSEE
260 270 280 290 300
EIVSFVEEHR RSTLRKLKPE SMYETWEDDL DGIHIVAFAE EADPDGYEFL
310 320 330 340 350
ETLKAVAQDN TENPDLSIIW IDPDDFPLLV PYWEKTFDID LSAPQIGVVN
360 370 380 390 400
VTDADSIWME MDNEEDLPSA DELEDWLEDV LEGEINTEDD DDDDDDDDDD

DDDDD
Length:405
Mass (Da):46,378
Last modified:July 27, 2011 - v3
Checksum:i813A8DD681434E59
GO

Sequence cautioni

The sequence AAC63616 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93291 mRNA Translation: AAC63616.1 Different initiation.
AC074310 Genomic DNA No translation available.
CCDSiCCDS35781.2
RefSeqiNP_033943.2, NM_009813.2
UniGeneiMm.12829

Genome annotation databases

EnsembliENSMUST00000003554; ENSMUSP00000003554; ENSMUSG00000007122
GeneIDi12372
KEGGimmu:12372
UCSCiuc007dqa.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCASQ1_MOUSE
AccessioniPrimary (citable) accession number: O09165
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 128 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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