Reviewed, UniProtKB/Swiss-Prot O09160 (FUT1_MOUSE)

Names and origin

Protein names

Recommended name:
    Galactoside 2-alpha-L-fucosyltransferase 1
    EC=2.4.1.69
Alternative name(s):
    GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 1
    Alpha(1,2)FT 1
    Fucosyltransferase 1

Gene names

Name:

Fut1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Membrane-bound form in trans cisternae of Golgi.
Tissue specificity	In the adult, highly expressed in pancreas, testis and epididymis and to a lesser extent in thymus, lung, stomach, small intestine, colon, spleen and uterus. Not expressed in brain, heart, skeletal muscle, kidney, liver and bone marrow.
Miscellaneous	In mouse, there are three genes (Fut1, Fut2 and Sec1) which encode galactoside 2-L-fucosyltransferase.
Sequence similarities	Belongs to the glycosyltransferase 11 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galactoside 2-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Galactoside 2-alpha-L-fucosyltransferase 1		PRO_0000149098
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 26	18	Signal-anchor for type II membrane protein Potential
	Topological domain	27 – 376	350	Lumenal Potential
Amino acid modifications
	Glycosylation	67	1	N-linked (GlcNAc...) Potential
	Glycosylation	302	1	N-linked (GlcNAc...) Potential
	Glycosylation	328	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O09160-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 21F1D24CFE204106	FASTA	376	42,255
	10 20 30 40 50 60 MWTPSRRQLC LTFLLVCVLS AGSFFFHLNG GNFFRNGLTL SVLCSDYHLL KSPVAMVCLP 70 80 90 100 110 120 HPLQTSNGSP SCPEQSSSLS GTWTITPGGR FGNQMGQYAT LLALAQLNGR QAFIQPEMHA 130 140 150 160 170 180 ALAPVFRISL PVLDPEVDSL TPWQHLVLHD WMSEEYSHLE DPFLKLSGFP CSWTFFHHLR 190 200 210 220 230 240 EQIRREFTLH NHLREGAQYL LSGLRIGPAS PAHTFVGVHV RRGDYLEVMP NRWKGVVGDR 250 260 270 280 290 300 AYLQQAMDWF RARHKDPIFV VTSNGMKWCL ENIDTSHGDV VFAGNGQEGT PGKDFALLTQ 310 320 330 340 350 360 CNHTIMTIGT FGFWAAYLAG GDTVYLANFT LPDSEFLKIF RPEAAFLPEW VGINADLSPL 370 QAQFDPWKPD SLFRLV

References

[1]	"Molecular cloning, chromosomal assignment and tissue-specific expression of a murine alpha(1,2)fucosyltransferase expressed in thymic and epididymal epithelial cells." Domino S.E., Hiraiwa N., Lowe J.B. Biochem. J. 327:105-115(1997) [PubMed: 9355741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NIH Swiss.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

Fucosyltransferase 1

Cross-references

Sequence databases
EMBL GenBank DDBJ	U90553 Genomic DNA. Translation: AAC53492.1.
IPI	IPI00226204.
UniGene	Mm.56933
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O09160.
Protein family/group databases
CAZy	GT11. Glycosyltransferase Family 11.
PTM databases
PhosphoSite	O09160.
Proteomic databases
PRIDE	O09160.
Genome annotation databases
Ensembl	ENSMUST00000008605; ENSMUSP00000008605; ENSMUSG00000008461; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:109375. Fut1.
Phylogenomic databases
eggNOG	roNOG04831.
HOVERGEN	O09160.
InParanoid	O09160.
Enzyme and pathway databases
BRENDA	2.4.1.69. 244.
Gene expression databases
ArrayExpress	O09160.
Bgee	O09160.
CleanEx	MM_FUT1.
Genevestigator	O09160.
GermOnline	ENSMUSG00000008461. Mus musculus.
Family and domain databases
InterPro	IPR002516. Glyco_trans_11. [Graphical view]
PANTHER	PTHR11927. Glyco_trans_11. 1 hit.
Pfam	PF01531. Glyco_transf_11. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

FUT1_MOUSE

Accession

Primary (citable) accession number: O09160

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	January 19, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)