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Protein

Lysosomal alpha-mannosidase

Gene

Man2b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover.

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi72ZincBy similarity1
Metal bindingi74ZincBy similarity1
Active sitei196NucleophileBy similarity1
Metal bindingi196ZincBy similarity1
Metal bindingi446ZincBy similarity1

GO - Molecular functioni

  • alpha-mannosidase activity Source: MGI
  • mannose binding Source: Ensembl
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cellular protein modification process Source: MGI
  • learning or memory Source: MGI
  • mannose metabolic process Source: MGI
  • protein deglycosylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.2.1.24. 3474.
ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-8853383. Lysosomal oligosaccharide catabolism.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal alpha-mannosidase (EC:3.2.1.24)
Short name:
Laman
Alternative name(s):
Lysosomal acid alpha-mannosidase
Mannosidase alpha class 2B member 1
Mannosidase alpha-B
Gene namesi
Name:Man2b1
Synonyms:Laman, Man2b, Manb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:107286. Man2b1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 49Sequence analysisAdd BLAST49
ChainiPRO_000001207650 – 1013Lysosomal alpha-mannosidaseAdd BLAST964

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi55 ↔ 358By similarity
Disulfide bondi268 ↔ 273By similarity
Glycosylationi310N-linked (GlcNAc...)Sequence analysis1
Glycosylationi345N-linked (GlcNAc...)Sequence analysis1
Glycosylationi367N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi412 ↔ 472By similarity
Glycosylationi489N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi493 ↔ 501By similarity
Glycosylationi497N-linked (GlcNAc...)Sequence analysis1
Glycosylationi544N-linked (GlcNAc...)Sequence analysis1
Glycosylationi633N-linked (GlcNAc...)Sequence analysis1
Glycosylationi646N-linked (GlcNAc...)Sequence analysis1
Glycosylationi693N-linked (GlcNAc...)Sequence analysis1
Glycosylationi767N-linked (GlcNAc...)Sequence analysis1
Glycosylationi931N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO09159.
MaxQBiO09159.
PaxDbiO09159.
PeptideAtlasiO09159.
PRIDEiO09159.

PTM databases

iPTMnetiO09159.
PhosphoSitePlusiO09159.

Expressioni

Gene expression databases

BgeeiENSMUSG00000005142.
CleanExiMM_MAN2B1.
GenevisibleiO09159. MM.

Interactioni

Protein-protein interaction databases

IntActiO09159. 2 interactors.
MINTiMINT-1675875.
STRINGi10090.ENSMUSP00000034121.

Structurei

3D structure databases

ProteinModelPortaliO09159.
SMRiO09159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1959. Eukaryota.
ENOG410XQMZ. LUCA.
GeneTreeiENSGT00510000046304.
HOGENOMiHOG000007676.
HOVERGENiHBG052391.
InParanoidiO09159.
KOiK12311.
OMAiSHVEFEW.
OrthoDBiEOG091G02IS.
TreeFamiTF313840.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR013780. Glyco_hydro_b.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O09159-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTGPLTSGV RAGGGNTGWL WMSSCNLGSP VLPISFLFWL LLAAPGARAA
60 70 80 90 100
GYKTCPPTKP GMLNVHLLPH THDDVGWLKT VDQYYYGILS DVQHASVQYI
110 120 130 140 150
LDSVVSSLLE KPTRRFIYVE MAFFSRWWKQ QTSATQDAVR NLVRQGRLEF
160 170 180 190 200
VNGGWVMNDE AATHYGAIVD QMTLGLRFLQ DTFGSDGLPR VAWHIDPFGH
210 220 230 240 250
SREQASLFAQ MGFDGFFLGR IDYQDKLNRK KKLRMEELWR ASDSLEPPAA
260 270 280 290 300
DLFTGVLPNN YNPPKYLCWD VLCTDPPVVD NPRSPEFNAK TLVNYFLKLA
310 320 330 340 350
SSQKGFYRTN HTVMTMGSDF HYENANMWFK NMDKLIRLVN AQQVNGSLVH
360 370 380 390 400
VLYSTPTCYL WELNKANLTW TVKEDDFFPY ADGPHMFWTG YFSSRPALKR
410 420 430 440 450
YERLSYNFLQ VCNQLEALVG PEANVGPYGS GDSAPLQEAM AVLQHHDAVS
460 470 480 490 500
GTARQNVVND YARQLAAGWG PCEVLVSNAL ARLSHYKQNF SFCRELNISI
510 520 530 540 550
CPVSQTSERF QVTLYNPLGR KVDQMVRLPV YEGNFIVKDP HDKNISSNVV
560 570 580 590 600
MVPSYYSETY QWELLFPASV PALGFSTYSV AKMSDLNHQA HNLLSRPRKH
610 620 630 640 650
KSHHVLVIEN KYMRATFDSG TGLLMKIENL EQNLSLPVSQ GFFWYNASVG
660 670 680 690 700
DEESSQASGA YIFRPNVGKP IPVSRWAQIS LVKTALVQEV HQNFSAWCSQ
710 720 730 740 750
VIRLYKGQRH LELEWTVGPI PVRDDWGKEV ISRFDTPMKT KGQFFTDSNG
760 770 780 790 800
REILKRRDDY RPTWTLNQTE PVAGNYYPVN TRIYITDGQM QLTVLTDRSQ
810 820 830 840 850
GGSSLQDGSL ELMVHRRLLV DDDRGVSEPL LETDTGDKVR GRHLVLLSSV
860 870 880 890 900
SDAAARHRLL AEQEVLAPQV VLSLGGSSPY HSRATPKTQF SGLRQELPPQ
910 920 930 940 950
VHLLTLARWG PKMLLLRLEH QFALKEDSDR NLSSPVTLNV QNLFQTFTIN
960 970 980 990 1000
YLQETTLAAN QPLSRASRLK WMTNTGPTSY PEPSKLDPTS VTLKPMEIRT
1010
FLASVQWQEH RPA
Length:1,013
Mass (Da):114,648
Last modified:July 27, 2011 - v4
Checksum:iA83B5397D9D38D31
GO

Sequence cautioni

The sequence AAC09470 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAC53369 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45 – 49PGARA → LASG in AAC53369 (PubMed:9355733).Curated5
Sequence conflicti45 – 49PGARA → LASG in AAC78560 (PubMed:9799835).Curated5
Sequence conflicti344V → A in AAC53369 (PubMed:9355733).Curated1
Sequence conflicti344V → A in AAC78560 (PubMed:9799835).Curated1
Sequence conflicti344V → A in AAH05430 (PubMed:15489334).Curated1
Sequence conflicti344V → A in AAC09470 (PubMed:9305783).Curated1
Sequence conflicti980Y → F in AAC53369 (PubMed:9355733).Curated1
Sequence conflicti980Y → F in AAC78560 (PubMed:9799835).Curated1
Sequence conflicti980Y → F in AAH05430 (PubMed:15489334).Curated1
Sequence conflicti980Y → F in AAC09470 (PubMed:9305783).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29947 mRNA. Translation: AAC53369.1. Different initiation.
AF044192
, AF044174, AF044175, AF044176, AF044177, AF044178, AF044179, AF044180, AF044181, AF044182, AF044183, AF044184, AF044185, AF044186, AF044187, AF044188, AF044189, AF044190, AF044191 Genomic DNA. Translation: AAC78560.1.
AK147928 mRNA. Translation: BAE28235.1.
AK004817 mRNA. Translation: BAB23588.1.
BC005430 mRNA. Translation: AAH05430.1.
U87240 mRNA. Translation: AAC09470.1. Different initiation.
CCDSiCCDS22494.1.
PIRiT42385.
RefSeqiNP_034894.2. NM_010764.2.
UniGeneiMm.4219.

Genome annotation databases

EnsembliENSMUST00000034121; ENSMUSP00000034121; ENSMUSG00000005142.
GeneIDi17159.
KEGGimmu:17159.
UCSCiuc009mpk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29947 mRNA. Translation: AAC53369.1. Different initiation.
AF044192
, AF044174, AF044175, AF044176, AF044177, AF044178, AF044179, AF044180, AF044181, AF044182, AF044183, AF044184, AF044185, AF044186, AF044187, AF044188, AF044189, AF044190, AF044191 Genomic DNA. Translation: AAC78560.1.
AK147928 mRNA. Translation: BAE28235.1.
AK004817 mRNA. Translation: BAB23588.1.
BC005430 mRNA. Translation: AAH05430.1.
U87240 mRNA. Translation: AAC09470.1. Different initiation.
CCDSiCCDS22494.1.
PIRiT42385.
RefSeqiNP_034894.2. NM_010764.2.
UniGeneiMm.4219.

3D structure databases

ProteinModelPortaliO09159.
SMRiO09159.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO09159. 2 interactors.
MINTiMINT-1675875.
STRINGi10090.ENSMUSP00000034121.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

PTM databases

iPTMnetiO09159.
PhosphoSitePlusiO09159.

Proteomic databases

EPDiO09159.
MaxQBiO09159.
PaxDbiO09159.
PeptideAtlasiO09159.
PRIDEiO09159.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034121; ENSMUSP00000034121; ENSMUSG00000005142.
GeneIDi17159.
KEGGimmu:17159.
UCSCiuc009mpk.1. mouse.

Organism-specific databases

CTDi4125.
MGIiMGI:107286. Man2b1.

Phylogenomic databases

eggNOGiKOG1959. Eukaryota.
ENOG410XQMZ. LUCA.
GeneTreeiENSGT00510000046304.
HOGENOMiHOG000007676.
HOVERGENiHBG052391.
InParanoidiO09159.
KOiK12311.
OMAiSHVEFEW.
OrthoDBiEOG091G02IS.
TreeFamiTF313840.

Enzyme and pathway databases

BRENDAi3.2.1.24. 3474.
ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-8853383. Lysosomal oligosaccharide catabolism.

Miscellaneous databases

ChiTaRSiMan2b1. mouse.
PROiO09159.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005142.
CleanExiMM_MAN2B1.
GenevisibleiO09159. MM.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR013780. Glyco_hydro_b.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMA2B1_MOUSE
AccessioniPrimary (citable) accession number: O09159
Secondary accession number(s): O55037
, Q3UGH3, Q64443, Q9DBQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 141 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.