Lysosomal alpha-mannosidase precursor - Mus musculus (Mouse)

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O09159 (MA2B1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Lysosomal alpha-mannosidase
Short name=Laman
EC=3.2.1.24

Alternative name(s):
Lysosomal acid alpha-mannosidase
Mannosidase alpha class 2B member 1
Mannosidase alpha-B

Gene names

Name:	Man2b1
Synonyms:	Laman, Man2b, Manb

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1013 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover.
Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the glycosyl hydrolase 38 family.
Sequence caution	The sequence AAC09470.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAC53369.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	learning or memory Inferred from mutant phenotype PubMed 16014715. Source: MGI mannose metabolic process Inferred from direct assay Ref.5 Ref.1. Source: MGI
Cellular_component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alpha-mannosidase activity Inferred from direct assay Ref.5 Ref.1. Source: MGI mannose binding Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

49

Potential

Chain

964

Lysosomal alpha-mannosidase

PRO_0000012076

Sites

Active site

1

Nucleophile By similarity

Metal binding

1

Zinc By similarity

Metal binding

1

Zinc By similarity

Metal binding

1

Zinc By similarity

Metal binding

1

Zinc By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Disulfide bond

By similarity

Disulfide bond

By similarity

Disulfide bond

By similarity

Disulfide bond

By similarity

Experimental info

Sequence conflict

5

PGARA → LASG in AAC53369. Ref.1

Sequence conflict

5

PGARA → LASG in AAC78560. Ref.2

Sequence conflict

1

V → A in AAC53369. Ref.1

Sequence conflict

1

V → A in AAC78560. Ref.2

Sequence conflict

1

V → A in AAH05430. Ref.4

Sequence conflict

1

V → A in AAC09470. Ref.5

Sequence conflict

1

Y → F in AAC53369. Ref.1

Sequence conflict

1

Y → F in AAC78560. Ref.2

Sequence conflict

1

Y → F in AAH05430. Ref.4

Sequence conflict

1

Y → F in AAC09470. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

O09159 [UniParc].

Last modified July 27, 2011. Version 4.
Checksum: A83B5397D9D38D31
Show »

1,013

114,648

        10         20         30         40         50         60 
MGTGPLTSGV RAGGGNTGWL WMSSCNLGSP VLPISFLFWL LLAAPGARAA GYKTCPPTKP 

        70         80         90        100        110        120 
GMLNVHLLPH THDDVGWLKT VDQYYYGILS DVQHASVQYI LDSVVSSLLE KPTRRFIYVE 

       130        140        150        160        170        180 
MAFFSRWWKQ QTSATQDAVR NLVRQGRLEF VNGGWVMNDE AATHYGAIVD QMTLGLRFLQ 

       190        200        210        220        230        240 
DTFGSDGLPR VAWHIDPFGH SREQASLFAQ MGFDGFFLGR IDYQDKLNRK KKLRMEELWR 

       250        260        270        280        290        300 
ASDSLEPPAA DLFTGVLPNN YNPPKYLCWD VLCTDPPVVD NPRSPEFNAK TLVNYFLKLA 

       310        320        330        340        350        360 
SSQKGFYRTN HTVMTMGSDF HYENANMWFK NMDKLIRLVN AQQVNGSLVH VLYSTPTCYL 

       370        380        390        400        410        420 
WELNKANLTW TVKEDDFFPY ADGPHMFWTG YFSSRPALKR YERLSYNFLQ VCNQLEALVG 

       430        440        450        460        470        480 
PEANVGPYGS GDSAPLQEAM AVLQHHDAVS GTARQNVVND YARQLAAGWG PCEVLVSNAL 

       490        500        510        520        530        540 
ARLSHYKQNF SFCRELNISI CPVSQTSERF QVTLYNPLGR KVDQMVRLPV YEGNFIVKDP 

       550        560        570        580        590        600 
HDKNISSNVV MVPSYYSETY QWELLFPASV PALGFSTYSV AKMSDLNHQA HNLLSRPRKH 

       610        620        630        640        650        660 
KSHHVLVIEN KYMRATFDSG TGLLMKIENL EQNLSLPVSQ GFFWYNASVG DEESSQASGA 

       670        680        690        700        710        720 
YIFRPNVGKP IPVSRWAQIS LVKTALVQEV HQNFSAWCSQ VIRLYKGQRH LELEWTVGPI 

       730        740        750        760        770        780 
PVRDDWGKEV ISRFDTPMKT KGQFFTDSNG REILKRRDDY RPTWTLNQTE PVAGNYYPVN 

       790        800        810        820        830        840 
TRIYITDGQM QLTVLTDRSQ GGSSLQDGSL ELMVHRRLLV DDDRGVSEPL LETDTGDKVR 

       850        860        870        880        890        900 
GRHLVLLSSV SDAAARHRLL AEQEVLAPQV VLSLGGSSPY HSRATPKTQF SGLRQELPPQ 

       910        920        930        940        950        960 
VHLLTLARWG PKMLLLRLEH QFALKEDSDR NLSSPVTLNV QNLFQTFTIN YLQETTLAAN 

       970        980        990       1000       1010 
QPLSRASRLK WMTNTGPTSY PEPSKLDPTS VTLKPMEIRT FLASVQWQEH RPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lysosomal alpha-mannosidases of mouse tissues: characteristics of the isoenzymes, and cloning and expression of a full-length cDNA." Beccari T., Appolloni M.G., Costanzi E., Stinchi S., Stirling J.L., Della Fazia M.A., Servillo G., Viola M.P., Orlacchio A. Biochem. J. 327:45-49(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Macrophage.
[2]	"Promoter characterization and structure of the gene encoding mouse lysosomal alpha-d-mannosidase." Stinchi S., Orlacchio A., Costanzi E., Stirling J.L., Menghini A.R., Orlacchio A., Beccari T. Mamm. Genome 9:869-873(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland.
[5]	"Cloning, expression, purification, and characterization of the murine lysosomal acid alpha-mannosidase." Merkle R.K., Zhang Y., Ruest P.J., Lal A., Liao Y.-F., Moremen K.W. Biochim. Biophys. Acta 1336:132-146(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-1013. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U29947 mRNA. Translation: AAC53369.1. Different initiation. AF044192 AF044191 Genomic DNA. Translation: AAC78560.1. AK147928 mRNA. Translation: BAE28235.1. AK004817 mRNA. Translation: BAB23588.1. BC005430 mRNA. Translation: AAH05430.1. U87240 mRNA. Translation: AAC09470.1. Different initiation.
IPI	IPI00381303.
PIR	T42385.
RefSeq	NP_034894.2. NM_010764.2.
UniGene	Mm.4219.
3D structure databases
ProteinModelPortal	O09159.
SMR	O09159. Positions 51-341, 349-421, 431-583, 606-875, 886-1007.
ModBase	Search...
Protein-protein interaction databases
IntAct	O09159. 1 interaction.
Protein family/group databases
CAZy	GH38. Glycoside Hydrolase Family 38.
Proteomic databases
PaxDb	O09159.
PRIDE	O09159.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034121; ENSMUSP00000034121; ENSMUSG00000005142.
GeneID	17159.
KEGG	mmu:17159.
Organism-specific databases
CTD	4125.
MGI	MGI:107286. Man2b1.
Phylogenomic databases
eggNOG	COG0383.
GeneTree	ENSGT00510000046304.
HOGENOM	HOG000007676.
HOVERGEN	HBG052391.
InParanoid	Q3UGH3.
KO	K12311.
OMA	NMQLTVL.
OrthoDB	EOG4DV5KJ.
Gene expression databases
Bgee	O09159.
CleanEx	MM_MAN2B1.
Genevestigator	O09159.
GermOnline	ENSMUSG00000005142. Mus musculus.
Family and domain databases
Gene3D	2.60.40.1180. 1 hit. 3.20.110.10. 1 hit.
InterPro	IPR011013. Gal_mutarotase_SF_dom. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR013780. Glyco_hydro_13_b. IPR027291. Glyco_hydro_38/57_N. IPR011682. Glyco_hydro_38_C. IPR015341. Glyco_hydro_38_cen_dom. IPR000602. Glyco_hydro_38_N. [Graphical view]
Pfam	PF09261. Alpha-mann_mid. 1 hit. PF01074. Glyco_hydro_38. 1 hit. PF07748. Glyco_hydro_38C. 1 hit. [Graphical view]
SMART	SM00872. Alpha-mann_mid. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	MAN2B1. mouse.
NextBio	291430.
SOURCE	Search...

Entry information

Entry name

MA2B1_MOUSE

Accession

Primary (citable) accession number: O09159
Secondary accession number(s): O55037

expand/collapse secondary AC list

Q9DBQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 109 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents