Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor E2F1

Gene

E2f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters.By similarity

Enzyme regulationi

BIRC2/c-IAP1 stimulates its transcriptional activity.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi108 – 192Sequence analysisAdd BLAST85

GO - Molecular functioni

  • core promoter binding Source: Ensembl
  • protein kinase binding Source: RGD
  • sequence-specific DNA binding Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-113510. E2F mediated regulation of DNA replication.
R-RNO-1538133. G0 and Early G1.
R-RNO-68689. CDC6 association with the ORC:origin complex.
R-RNO-68911. G2 Phase.
R-RNO-69205. G1/S-Specific Transcription.
R-RNO-69231. Cyclin D associated events in G1.
R-RNO-69298. Association of licensing factors with the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F1
Short name:
E2F-1
Gene namesi
Name:E2f1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi728892. E2f1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • nuclear chromatin Source: Ensembl
  • nucleus Source: RGD
  • Rb-E2F complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002194631 – 432Transcription factor E2F1Add BLAST432

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei115N6-acetyllysineBy similarity1
Modified residuei118N6-acetyllysineBy similarity1
Modified residuei123N6-acetyllysineBy similarity1
Modified residuei370PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis (By similarity).By similarity
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO09139.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016708.
ExpressionAtlasiO09139. baseline and differential.
GenevisibleiO09139. RN.

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1 and EAPP. Interacts with ARID3A. Interacts with TRIM28; the interaction inhibits E2F1 acetylation through recruiting HDAC1 and represses its transcriptional activity. Interaction with KAT2B; the interaction acetylates E2F1 enhancing its DNA-binding and transcriptional activity. Interacts with BIRC2/c-IAP1 (via BIR domains). The complex TFDP1:E2F1 interacts with CEBPA; the interaction prevents CEBPA binding to target genes promoters and represses its transcriptional activity.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Rb1P335682EBI-1211101,EBI-1162932

GO - Molecular functioni

  • protein kinase binding Source: RGD

Protein-protein interaction databases

IntActiO09139. 1 interactor.
STRINGi10116.ENSRNOP00000022428.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 106Cyclin A:CDK2 bindingBy similarityAdd BLAST42
Regioni87 – 189Interaction with BIRC2/c-IAP1By similarityAdd BLAST103
Regioni151 – 172Leucine-zipperBy similarityAdd BLAST22
Regioni190 – 377Required for interaction with TRIM28By similarityAdd BLAST188
Regioni193 – 282DimerizationSequence analysisAdd BLAST90
Regioni363 – 432TransactivationBy similarityAdd BLAST70
Regioni404 – 421RB1 bindingBy similarityAdd BLAST18

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi156 – 192DEF boxBy similarityAdd BLAST37

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2577. Eukaryota.
ENOG410XNYI. LUCA.
GeneTreeiENSGT00550000074403.
HOGENOMiHOG000232045.
InParanoidiO09139.
KOiK17454.
OMAiHVREDFS.
OrthoDBiEOG091G087U.

Family and domain databases

CDDicd14660. E2F_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR032198. E2F_CC-MB.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PfamiPF16421. E2F_CC-MB. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
SMARTiSM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Fragment.

O09139-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVAPAGGQH APALEALLGA GALRLLDSSQ IVIISTAPDV GAPQVPTGPA
60 70 80 90 100
APPAGPRDPD VLLFATPQAP RPAPSAPRPA LGRPPVKRRL DLETDHQYLA
110 120 130 140 150
GSSGPFRGRG RHPGKGVKSP GEKSRYETSL NLTTKRFLEL LSHSADGVVD
160 170 180 190 200
LNWAAEVLKV QKRRIYDITN VLEGIQLIAK KSKNHIQWLG SRTMVGIGQR
210 220 230 240 250
LEGLTQDLQQ LQESEQQLDH LMHICTTQLQ LLSEDSDIQR LAYVTCQDLR
260 270 280 290 300
SIADPAEQMV IVIKAPPETQ LQAVDSAETF QISLKSKQGP IDVFLCPEES
310 320 330 340 350
AEGISPGRTS YQETSGEDRN ADSGTAGPPP SPPSTSPTLD PSQSLLGLEQ
360 370 380 390 400
EAVLPRIGNL RAPMEEDRLS PLVAADSLLE HVKEDFSGLL PGEFISLSPP
410 420 430
HEAVDYHFGL EEGEGIRDLF DCDFGDLTPL DF
Length:432
Mass (Da):46,548
Last modified:April 1, 2015 - v2
Checksum:i4E401ADF6E20C13C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63165 mRNA. Translation: BAA09641.1.
RefSeqiNP_001094248.1. NM_001100778.1.
UniGeneiRn.72471.

Genome annotation databases

EnsembliENSRNOT00000022428; ENSRNOP00000022428; ENSRNOG00000016708.
GeneIDi399489.
KEGGirno:399489.
UCSCiRGD:728892. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63165 mRNA. Translation: BAA09641.1.
RefSeqiNP_001094248.1. NM_001100778.1.
UniGeneiRn.72471.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO09139. 1 interactor.
STRINGi10116.ENSRNOP00000022428.

Proteomic databases

PaxDbiO09139.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022428; ENSRNOP00000022428; ENSRNOG00000016708.
GeneIDi399489.
KEGGirno:399489.
UCSCiRGD:728892. rat.

Organism-specific databases

CTDi1869.
RGDi728892. E2f1.

Phylogenomic databases

eggNOGiKOG2577. Eukaryota.
ENOG410XNYI. LUCA.
GeneTreeiENSGT00550000074403.
HOGENOMiHOG000232045.
InParanoidiO09139.
KOiK17454.
OMAiHVREDFS.
OrthoDBiEOG091G087U.

Enzyme and pathway databases

ReactomeiR-RNO-113510. E2F mediated regulation of DNA replication.
R-RNO-1538133. G0 and Early G1.
R-RNO-68689. CDC6 association with the ORC:origin complex.
R-RNO-68911. G2 Phase.
R-RNO-69205. G1/S-Specific Transcription.
R-RNO-69231. Cyclin D associated events in G1.
R-RNO-69298. Association of licensing factors with the pre-replicative complex.

Gene expression databases

BgeeiENSRNOG00000016708.
ExpressionAtlasiO09139. baseline and differential.
GenevisibleiO09139. RN.

Family and domain databases

CDDicd14660. E2F_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR032198. E2F_CC-MB.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PfamiPF16421. E2F_CC-MB. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
SMARTiSM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiE2F1_RAT
AccessioniPrimary (citable) accession number: O09139
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 1, 2015
Last modified: October 5, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.