Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Netrin-1

Gene

Ntn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis (By similarity).By similarity2 Publications

GO - Biological processi

  • anterior/posterior axon guidance Source: MGI
  • apoptotic process Source: UniProtKB-KW
  • axon guidance Source: MGI
  • axonogenesis Source: MGI
  • Cdc42 protein signal transduction Source: UniProtKB
  • establishment of nucleus localization Source: MGI
  • inner ear morphogenesis Source: MGI
  • mammary gland development Source: MGI
  • mammary gland duct morphogenesis Source: MGI
  • negative regulation of axon extension Source: MGI
  • neuron migration Source: MGI
  • positive regulation of axon extension Source: MGI
  • positive regulation of cell motility Source: UniProtKB
  • positive regulation of cell proliferation Source: MGI
  • Ras protein signal transduction Source: UniProtKB
  • regulation of cell migration Source: MGI
  • single organismal cell-cell adhesion Source: MGI
  • substrate-dependent cell migration, cell extension Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiR-MMU-373752. Netrin-1 signaling.
R-MMU-376172. DSCAM interactions.
R-MMU-418885. DCC mediated attractive signaling.
R-MMU-418886. Netrin mediated repulsion signals.
R-MMU-418890. Role of second messengers in netrin-1 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Netrin-1
Gene namesi
Name:Ntn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:105088. Ntn1.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: MGI
  • cell periphery Source: MGI
  • cytoplasm Source: MGI
  • extracellular region Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000001708325 – 604Netrin-1Add BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi95N-linked (GlcNAc...)Sequence analysis1
Glycosylationi116N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi119 ↔ 152By similarity
Glycosylationi131N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi285 ↔ 294By similarity
Disulfide bondi287 ↔ 304By similarity
Disulfide bondi306 ↔ 315By similarity
Disulfide bondi318 ↔ 338By similarity
Disulfide bondi341 ↔ 350By similarity
Disulfide bondi343 ↔ 368By similarity
Disulfide bondi371 ↔ 380By similarity
Disulfide bondi383 ↔ 401By similarity
Disulfide bondi404 ↔ 416By similarity
Disulfide bondi406 ↔ 423By similarity
Glycosylationi417N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi425 ↔ 434By similarity
Disulfide bondi437 ↔ 451By similarity
Disulfide bondi472 ↔ 544By similarity
Disulfide bondi491 ↔ 601By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO09118.
PaxDbiO09118.
PeptideAtlasiO09118.
PRIDEiO09118.

PTM databases

PhosphoSitePlusiO09118.

Expressioni

Tissue specificityi

In the embryo, widely expressed in the developing nervous system and in mesodermal tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000020902.
CleanExiMM_NTN1.
ExpressionAtlasiO09118. baseline and differential.
GenevisibleiO09118. MM.

Interactioni

Subunit structurei

Binds to its receptors; DCC, UNC5A, UNC5B, UNC5C and probably UNC5D (By similarity). Binds to its receptor; DSCAM.By similarity

Protein-protein interaction databases

IntActiO09118. 3 interactors.
STRINGi10090.ENSMUSP00000021284.

Structurei

Secondary structure

1604
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi40 – 42Combined sources3
Turni58 – 61Combined sources4
Beta strandi65 – 67Combined sources3
Beta strandi75 – 81Combined sources7
Beta strandi83 – 85Combined sources3
Beta strandi88 – 94Combined sources7
Turni99 – 101Combined sources3
Helixi105 – 108Combined sources4
Beta strandi132 – 153Combined sources22
Beta strandi157 – 166Combined sources10
Beta strandi172 – 177Combined sources6
Helixi181 – 185Combined sources5
Beta strandi197 – 199Combined sources3
Helixi206 – 208Combined sources3
Beta strandi213 – 215Combined sources3
Beta strandi217 – 221Combined sources5
Turni222 – 225Combined sources4
Helixi227 – 234Combined sources8
Helixi236 – 241Combined sources6
Beta strandi243 – 253Combined sources11
Beta strandi275 – 285Combined sources11
Beta strandi294 – 296Combined sources3
Beta strandi302 – 304Combined sources3
Turni312 – 315Combined sources4
Beta strandi348 – 352Combined sources5
Helixi354 – 359Combined sources6
Beta strandi366 – 370Combined sources5
Beta strandi373 – 376Combined sources4
Turni377 – 380Combined sources4
Beta strandi387 – 389Combined sources3
Beta strandi401 – 403Combined sources3
Turni408 – 410Combined sources3
Turni418 – 420Combined sources3
Turni431 – 434Combined sources4
Beta strandi441 – 443Combined sources3
Beta strandi451 – 453Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OVEX-ray2.64A23-457[»]
ProteinModelPortaliO09118.
SMRiO09118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 284Laminin N-terminalPROSITE-ProRule annotationAdd BLAST238
Domaini285 – 340Laminin EGF-like 1PROSITE-ProRule annotationAdd BLAST56
Domaini341 – 403Laminin EGF-like 2PROSITE-ProRule annotationAdd BLAST63
Domaini404 – 453Laminin EGF-like 3PROSITE-ProRule annotationAdd BLAST50
Domaini472 – 601NTRPROSITE-ProRule annotationAdd BLAST130

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi530 – 532Cell attachment siteSequence analysis3

Sequence similaritiesi

Contains 3 laminin EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin N-terminal domain.PROSITE-ProRule annotation
Contains 1 NTR domain.PROSITE-ProRule annotation

Keywords - Domaini

Laminin EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3512. Eukaryota.
ENOG410XS7U. LUCA.
GeneTreeiENSGT00840000129717.
HOGENOMiHOG000286017.
HOVERGENiHBG006464.
InParanoidiO09118.
KOiK06843.
OMAiQVHILKA.
OrthoDBiEOG091G005L.
TreeFamiTF352481.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR002049. Laminin_EGF.
IPR008211. Laminin_N.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PfamiPF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF50242. SSF50242. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01248. EGF_LAM_1. 3 hits.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O09118-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRAVWEALA ALAAVACLVG AVRGGPGLSM FAGQAAQPDP CSDENGHPRR
60 70 80 90 100
CIPDFVNAAF GKDVRVSSTC GRPPARYCVV SERGEERLRS CHLCNSSDPK
110 120 130 140 150
KAHPPAFLTD LNNPHNLTCW QSENYLQFPH NVTLTLSLGK KFEVTYVSLQ
160 170 180 190 200
FCSPRPESMA IYKSMDYGRT WVPFQFYSTQ CRKMYNRPHR APITKQNEQE
210 220 230 240 250
AVCTDSHTDM RPLSGGLIAF STLDGRPSAH DFDNSPVLQD WVTATDIRVA
260 270 280 290 300
FSRLHTFGDE NEDDSELARD SYYYAVSDLQ VGGRCKCNGH AARCVRDRDD
310 320 330 340 350
SLVCDCRHNT AGPECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC
360 370 380 390 400
RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA
410 420 430 440 450
CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP
460 470 480 490 500
CIKIPVAPPT TAASSVEEPE DCDSYCKASK GKLKMNMKKY CRKDYAVQIH
510 520 530 540 550
ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIACKCPKIK
560 570 580 590 600
PLKKYLLLGN AEDSPDQSGI VADKSSLVIQ WRDTWARRLR KFQQREKKGK

CKKA
Length:604
Mass (Da):67,810
Last modified:July 27, 2011 - v3
Checksum:i135120DB6F0482F3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24Missing in AAC52971 (PubMed:8978605).Curated1
Sequence conflicti88L → V in AAC52971 (PubMed:8978605).Curated1
Sequence conflicti88L → V in AAD28602 (PubMed:10381568).Curated1
Sequence conflicti466V → A in AAD28602 (PubMed:10381568).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65418 mRNA. Translation: AAC52971.1.
AF128865 mRNA. Translation: AAD28602.1.
AL669842, AL606831, AL662894 Genomic DNA. Translation: CAI24775.1.
AL606831, AL662894, AL669842 Genomic DNA. Translation: CAI25691.1.
AL662894, AL606831, AL669842 Genomic DNA. Translation: CAI25793.1.
CH466601 Genomic DNA. Translation: EDL10446.1.
BC141294 mRNA. Translation: AAI41295.1.
U23505 mRNA. Translation: AAA87938.1.
CCDSiCCDS24864.1.
RefSeqiNP_032770.2. NM_008744.2.
UniGeneiMm.39095.

Genome annotation databases

EnsembliENSMUST00000021284; ENSMUSP00000021284; ENSMUSG00000020902.
ENSMUST00000108674; ENSMUSP00000104314; ENSMUSG00000020902.
GeneIDi18208.
KEGGimmu:18208.
UCSCiuc007jnm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65418 mRNA. Translation: AAC52971.1.
AF128865 mRNA. Translation: AAD28602.1.
AL669842, AL606831, AL662894 Genomic DNA. Translation: CAI24775.1.
AL606831, AL662894, AL669842 Genomic DNA. Translation: CAI25691.1.
AL662894, AL606831, AL669842 Genomic DNA. Translation: CAI25793.1.
CH466601 Genomic DNA. Translation: EDL10446.1.
BC141294 mRNA. Translation: AAI41295.1.
U23505 mRNA. Translation: AAA87938.1.
CCDSiCCDS24864.1.
RefSeqiNP_032770.2. NM_008744.2.
UniGeneiMm.39095.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OVEX-ray2.64A23-457[»]
ProteinModelPortaliO09118.
SMRiO09118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO09118. 3 interactors.
STRINGi10090.ENSMUSP00000021284.

PTM databases

PhosphoSitePlusiO09118.

Proteomic databases

MaxQBiO09118.
PaxDbiO09118.
PeptideAtlasiO09118.
PRIDEiO09118.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021284; ENSMUSP00000021284; ENSMUSG00000020902.
ENSMUST00000108674; ENSMUSP00000104314; ENSMUSG00000020902.
GeneIDi18208.
KEGGimmu:18208.
UCSCiuc007jnm.1. mouse.

Organism-specific databases

CTDi9423.
MGIiMGI:105088. Ntn1.

Phylogenomic databases

eggNOGiKOG3512. Eukaryota.
ENOG410XS7U. LUCA.
GeneTreeiENSGT00840000129717.
HOGENOMiHOG000286017.
HOVERGENiHBG006464.
InParanoidiO09118.
KOiK06843.
OMAiQVHILKA.
OrthoDBiEOG091G005L.
TreeFamiTF352481.

Enzyme and pathway databases

ReactomeiR-MMU-373752. Netrin-1 signaling.
R-MMU-376172. DSCAM interactions.
R-MMU-418885. DCC mediated attractive signaling.
R-MMU-418886. Netrin mediated repulsion signals.
R-MMU-418890. Role of second messengers in netrin-1 signaling.

Miscellaneous databases

PROiO09118.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020902.
CleanExiMM_NTN1.
ExpressionAtlasiO09118. baseline and differential.
GenevisibleiO09118. MM.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR002049. Laminin_EGF.
IPR008211. Laminin_N.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PfamiPF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF50242. SSF50242. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01248. EGF_LAM_1. 3 hits.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNET1_MOUSE
AccessioniPrimary (citable) accession number: O09118
Secondary accession number(s): B1ARR0, Q60832, Q9QY50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.