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O09118 (NET1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Netrin-1
Gene names
Name:Ntn1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length604 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis By similarity. Ref.1 Ref.2

Subunit structure

Binds to its receptors; DCC, UNC5A, UNC5B, UNC5C and probably UNC5D By similarity. Binds to its receptor; DSCAM. Ref.7

Subcellular location

Secretedextracellular spaceextracellular matrix Ref.6.

Tissue specificity

In the embryo, widely expressed in the developing nervous system and in mesodermal tissues. Ref.2

Sequence similarities

Contains 3 laminin EGF-like domains.

Contains 1 laminin N-terminal domain.

Contains 1 NTR domain.

Ontologies

Keywords
   Biological processApoptosis
   Cellular componentExtracellular matrix
Secreted
   DomainLaminin EGF-like domain
Repeat
Signal
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanterior/posterior axon guidance

Inferred from mutant phenotype PubMed 18479186. Source: MGI

apoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

axon guidance

Inferred from direct assay PubMed 12051827PubMed 12679031PubMed 17086203. Source: MGI

axonogenesis

Inferred from direct assay PubMed 10908620. Source: MGI

establishment of nucleus localization

Inferred from direct assay PubMed 12051827. Source: MGI

inner ear morphogenesis

Inferred from mutant phenotype PubMed 17616930. Source: MGI

mammary gland development

Inferred from genetic interaction PubMed 18425773. Source: MGI

mammary gland duct morphogenesis

Inferred from genetic interaction PubMed 16439476. Source: MGI

negative regulation of axon extension

Inferred from direct assay PubMed 10704383. Source: MGI

neuron migration

Inferred from mutant phenotype PubMed 14750959PubMed 16267219. Source: MGI

positive regulation of axon extension

Inferred from direct assay PubMed 10704383. Source: MGI

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 16481423. Source: MGI

regulation of cell migration

Inferred from direct assay PubMed 10704383. Source: MGI

single organismal cell-cell adhesion

Inferred from genetic interaction PubMed 16439476. Source: MGI

   Cellular_componentbasement membrane

Inferred from direct assay PubMed 18757743. Source: MGI

cell periphery

Inferred from sequence orthology PubMed 16455951. Source: MGI

cytoplasm

Inferred from direct assay PubMed 18425773. Source: MGI

extracellular region

Traceable author statement. Source: Reactome

   Molecular_functionprotein binding

Inferred from physical interaction Ref.7. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 604580Netrin-1
PRO_0000017083

Regions

Domain47 – 284238Laminin N-terminal
Domain285 – 34056Laminin EGF-like 1
Domain341 – 40363Laminin EGF-like 2
Domain404 – 45350Laminin EGF-like 3
Domain472 – 601130NTR
Motif530 – 5323Cell attachment site Potential

Amino acid modifications

Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1161N-linked (GlcNAc...) Potential
Glycosylation1311N-linked (GlcNAc...) Potential
Glycosylation4171N-linked (GlcNAc...) Potential
Disulfide bond119 ↔ 152 By similarity
Disulfide bond285 ↔ 294 By similarity
Disulfide bond287 ↔ 304 By similarity
Disulfide bond306 ↔ 315 By similarity
Disulfide bond318 ↔ 338 By similarity
Disulfide bond341 ↔ 350 By similarity
Disulfide bond343 ↔ 368 By similarity
Disulfide bond371 ↔ 380 By similarity
Disulfide bond383 ↔ 401 By similarity
Disulfide bond404 ↔ 416 By similarity
Disulfide bond406 ↔ 423 By similarity
Disulfide bond425 ↔ 434 By similarity
Disulfide bond437 ↔ 451 By similarity
Disulfide bond472 ↔ 544 By similarity
Disulfide bond491 ↔ 601 By similarity

Experimental info

Sequence conflict241Missing in AAC52971. Ref.1
Sequence conflict881L → V in AAC52971. Ref.1
Sequence conflict881L → V in AAD28602. Ref.2
Sequence conflict4661V → A in AAD28602. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O09118 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 135120DB6F0482F3

FASTA60467,810
        10         20         30         40         50         60 
MMRAVWEALA ALAAVACLVG AVRGGPGLSM FAGQAAQPDP CSDENGHPRR CIPDFVNAAF 

        70         80         90        100        110        120 
GKDVRVSSTC GRPPARYCVV SERGEERLRS CHLCNSSDPK KAHPPAFLTD LNNPHNLTCW 

       130        140        150        160        170        180 
QSENYLQFPH NVTLTLSLGK KFEVTYVSLQ FCSPRPESMA IYKSMDYGRT WVPFQFYSTQ 

       190        200        210        220        230        240 
CRKMYNRPHR APITKQNEQE AVCTDSHTDM RPLSGGLIAF STLDGRPSAH DFDNSPVLQD 

       250        260        270        280        290        300 
WVTATDIRVA FSRLHTFGDE NEDDSELARD SYYYAVSDLQ VGGRCKCNGH AARCVRDRDD 

       310        320        330        340        350        360 
SLVCDCRHNT AGPECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS 

       370        380        390        400        410        420 
GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA CKACDCHPVG AAGKTCNQTT 

       430        440        450        460        470        480 
GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSVEEPE DCDSYCKASK 

       490        500        510        520        530        540 
GKLKMNMKKY CRKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR 

       550        560        570        580        590        600 
DIACKCPKIK PLKKYLLLGN AEDSPDQSGI VADKSSLVIQ WRDTWARRLR KFQQREKKGK 


CKKA 

« Hide

References

« Hide 'large scale' references
[1]"Netrin-1 is required for commissural axon guidance in the developing vertebrate nervous system."
Serafini T., Colamarino S.A., Leonardo E.D., Wang H., Beddington R., Skarnes W.C., Tessier-Lavigne M.
Cell 87:1001-1014(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Tissue: Embryonic brain.
[2]"Divergent properties of mouse netrins."
Pueschel A.W.
Mech. Dev. 83:65-75(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"Capturing genes encoding membrane and secreted proteins important for mouse development."
Skarnes W.C., Moss J.E., Hurtley S.M., Beddington R.S.
Proc. Natl. Acad. Sci. U.S.A. 92:6592-6596(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 352-398, SUBCELLULAR LOCATION.
[7]"DSCAM is a netrin receptor that collaborates with DCC in mediating turning responses to netrin-1."
Ly A., Nikolaev A., Suresh G., Zheng Y., Tessier-Lavigne M., Stein E.
Cell 133:1241-1254(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DSCAM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U65418 mRNA. Translation: AAC52971.1.
AF128865 mRNA. Translation: AAD28602.1.
AL669842, AL606831, AL662894 Genomic DNA. Translation: CAI24775.1.
AL606831, AL662894, AL669842 Genomic DNA. Translation: CAI25691.1.
AL662894, AL606831, AL669842 Genomic DNA. Translation: CAI25793.1.
CH466601 Genomic DNA. Translation: EDL10446.1.
BC141294 mRNA. Translation: AAI41295.1.
U23505 mRNA. Translation: AAA87938.1.
CCDSCCDS24864.1.
RefSeqNP_032770.2. NM_008744.2.
UniGeneMm.39095.

3D structure databases

ProteinModelPortalO09118.
SMRO09118. Positions 41-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO09118. 2 interactions.
STRING10090.ENSMUSP00000021284.

PTM databases

PhosphoSiteO09118.

Proteomic databases

PRIDEO09118.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021284; ENSMUSP00000021284; ENSMUSG00000020902.
ENSMUST00000108674; ENSMUSP00000104314; ENSMUSG00000020902.
GeneID18208.
KEGGmmu:18208.
UCSCuc007jnm.1. mouse.

Organism-specific databases

CTD9423.
MGIMGI:105088. Ntn1.

Phylogenomic databases

eggNOGNOG244580.
GeneTreeENSGT00750000117313.
HOGENOMHOG000286017.
HOVERGENHBG006464.
InParanoidB1ARR0.
KOK06843.
OMADSYYYAV.
OrthoDBEOG7SR4KJ.
TreeFamTF352481.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.
REACT_223082. Cell-Cell communication.

Gene expression databases

ArrayExpressO09118.
BgeeO09118.
CleanExMM_NTN1.
GenevestigatorO09118.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
InterProIPR013032. EGF-like_CS.
IPR002049. EGF_laminin.
IPR008979. Galactose-bd-like.
IPR008211. Laminin_N.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PfamPF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTSM00643. C345C. 1 hit.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 1 hit.
SSF50242. SSF50242. 1 hit.
PROSITEPS00022. EGF_1. 2 hits.
PS01248. EGF_LAM_1. 3 hits.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio293590.
PROO09118.
SOURCESearch...

Entry information

Entry nameNET1_MOUSE
AccessionPrimary (citable) accession number: O09118
Secondary accession number(s): B1ARR0, Q60832, Q9QY50
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot