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Protein

Netrin-1

Gene

Ntn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis (By similarity).By similarity

GO - Biological processi

  • anterior/posterior axon guidance Source: MGI
  • apoptotic process Source: UniProtKB-KW
  • axon guidance Source: MGI
  • axonogenesis Source: MGI
  • establishment of nucleus localization Source: MGI
  • inner ear morphogenesis Source: MGI
  • mammary gland development Source: MGI
  • mammary gland duct morphogenesis Source: MGI
  • negative regulation of axon extension Source: MGI
  • neuron migration Source: MGI
  • positive regulation of axon extension Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • regulation of cell migration Source: MGI
  • single organismal cell-cell adhesion Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiREACT_271763. DSCAM interactions.
REACT_293956. Netrin-1 signaling.
REACT_295531. DCC mediated attractive signaling.
REACT_336806. Role of second messengers in netrin-1 signaling.
REACT_337259. Netrin mediated repulsion signals.

Names & Taxonomyi

Protein namesi
Recommended name:
Netrin-1
Gene namesi
Name:Ntn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:105088. Ntn1.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: MGI
  • cell periphery Source: MGI
  • cytoplasm Source: MGI
  • extracellular region Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 604580Netrin-1PRO_0000017083Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi119 ↔ 152By similarity
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi285 ↔ 294By similarity
Disulfide bondi287 ↔ 304By similarity
Disulfide bondi306 ↔ 315By similarity
Disulfide bondi318 ↔ 338By similarity
Disulfide bondi341 ↔ 350By similarity
Disulfide bondi343 ↔ 368By similarity
Disulfide bondi371 ↔ 380By similarity
Disulfide bondi383 ↔ 401By similarity
Disulfide bondi404 ↔ 416By similarity
Disulfide bondi406 ↔ 423By similarity
Glycosylationi417 – 4171N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi425 ↔ 434By similarity
Disulfide bondi437 ↔ 451By similarity
Disulfide bondi472 ↔ 544By similarity
Disulfide bondi491 ↔ 601By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO09118.
PRIDEiO09118.

PTM databases

PhosphoSiteiO09118.

Expressioni

Tissue specificityi

In the embryo, widely expressed in the developing nervous system and in mesodermal tissues.1 Publication

Gene expression databases

BgeeiO09118.
CleanExiMM_NTN1.
ExpressionAtlasiO09118. baseline and differential.
GenevisibleiO09118. MM.

Interactioni

Subunit structurei

Binds to its receptors; DCC, UNC5A, UNC5B, UNC5C and probably UNC5D (By similarity). Binds to its receptor; DSCAM.By similarity

Protein-protein interaction databases

IntActiO09118. 2 interactions.
STRINGi10090.ENSMUSP00000021284.

Structurei

Secondary structure

1
604
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi40 – 423Combined sources
Turni58 – 614Combined sources
Beta strandi65 – 673Combined sources
Beta strandi75 – 817Combined sources
Beta strandi83 – 853Combined sources
Beta strandi88 – 947Combined sources
Turni99 – 1013Combined sources
Helixi105 – 1084Combined sources
Beta strandi132 – 15322Combined sources
Beta strandi157 – 16610Combined sources
Beta strandi172 – 1776Combined sources
Helixi181 – 1855Combined sources
Beta strandi197 – 1993Combined sources
Helixi206 – 2083Combined sources
Beta strandi213 – 2153Combined sources
Beta strandi217 – 2215Combined sources
Turni222 – 2254Combined sources
Helixi227 – 2348Combined sources
Helixi236 – 2416Combined sources
Beta strandi243 – 25311Combined sources
Beta strandi275 – 28511Combined sources
Beta strandi294 – 2963Combined sources
Beta strandi302 – 3043Combined sources
Turni312 – 3154Combined sources
Beta strandi348 – 3525Combined sources
Helixi354 – 3596Combined sources
Beta strandi366 – 3705Combined sources
Beta strandi373 – 3764Combined sources
Turni377 – 3804Combined sources
Beta strandi387 – 3893Combined sources
Beta strandi401 – 4033Combined sources
Turni408 – 4103Combined sources
Turni418 – 4203Combined sources
Turni431 – 4344Combined sources
Beta strandi441 – 4433Combined sources
Beta strandi451 – 4533Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OVEX-ray2.64A23-457[»]
ProteinModelPortaliO09118.
SMRiO09118. Positions 39-453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 284238Laminin N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini285 – 34056Laminin EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini341 – 40363Laminin EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini404 – 45350Laminin EGF-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini472 – 601130NTRPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi530 – 5323Cell attachment siteSequence Analysis

Sequence similaritiesi

Contains 3 laminin EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin N-terminal domain.PROSITE-ProRule annotation
Contains 1 NTR domain.PROSITE-ProRule annotation

Keywords - Domaini

Laminin EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG244580.
GeneTreeiENSGT00780000121873.
HOGENOMiHOG000286017.
HOVERGENiHBG006464.
InParanoidiO09118.
KOiK06843.
OMAiKMYNKPN.
OrthoDBiEOG7SR4KJ.
TreeFamiTF352481.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR002049. EGF_laminin.
IPR008979. Galactose-bd-like.
IPR008211. Laminin_N.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PfamiPF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF50242. SSF50242. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01248. EGF_LAM_1. 3 hits.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O09118-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRAVWEALA ALAAVACLVG AVRGGPGLSM FAGQAAQPDP CSDENGHPRR
60 70 80 90 100
CIPDFVNAAF GKDVRVSSTC GRPPARYCVV SERGEERLRS CHLCNSSDPK
110 120 130 140 150
KAHPPAFLTD LNNPHNLTCW QSENYLQFPH NVTLTLSLGK KFEVTYVSLQ
160 170 180 190 200
FCSPRPESMA IYKSMDYGRT WVPFQFYSTQ CRKMYNRPHR APITKQNEQE
210 220 230 240 250
AVCTDSHTDM RPLSGGLIAF STLDGRPSAH DFDNSPVLQD WVTATDIRVA
260 270 280 290 300
FSRLHTFGDE NEDDSELARD SYYYAVSDLQ VGGRCKCNGH AARCVRDRDD
310 320 330 340 350
SLVCDCRHNT AGPECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC
360 370 380 390 400
RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA
410 420 430 440 450
CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP
460 470 480 490 500
CIKIPVAPPT TAASSVEEPE DCDSYCKASK GKLKMNMKKY CRKDYAVQIH
510 520 530 540 550
ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIACKCPKIK
560 570 580 590 600
PLKKYLLLGN AEDSPDQSGI VADKSSLVIQ WRDTWARRLR KFQQREKKGK

CKKA
Length:604
Mass (Da):67,810
Last modified:July 27, 2011 - v3
Checksum:i135120DB6F0482F3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241Missing in AAC52971 (PubMed:8978605).Curated
Sequence conflicti88 – 881L → V in AAC52971 (PubMed:8978605).Curated
Sequence conflicti88 – 881L → V in AAD28602 (PubMed:10381568).Curated
Sequence conflicti466 – 4661V → A in AAD28602 (PubMed:10381568).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65418 mRNA. Translation: AAC52971.1.
AF128865 mRNA. Translation: AAD28602.1.
AL669842, AL606831, AL662894 Genomic DNA. Translation: CAI24775.1.
AL606831, AL662894, AL669842 Genomic DNA. Translation: CAI25691.1.
AL662894, AL606831, AL669842 Genomic DNA. Translation: CAI25793.1.
CH466601 Genomic DNA. Translation: EDL10446.1.
BC141294 mRNA. Translation: AAI41295.1.
U23505 mRNA. Translation: AAA87938.1.
CCDSiCCDS24864.1.
RefSeqiNP_032770.2. NM_008744.2.
UniGeneiMm.39095.

Genome annotation databases

EnsembliENSMUST00000021284; ENSMUSP00000021284; ENSMUSG00000020902.
ENSMUST00000108674; ENSMUSP00000104314; ENSMUSG00000020902.
GeneIDi18208.
KEGGimmu:18208.
UCSCiuc007jnm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65418 mRNA. Translation: AAC52971.1.
AF128865 mRNA. Translation: AAD28602.1.
AL669842, AL606831, AL662894 Genomic DNA. Translation: CAI24775.1.
AL606831, AL662894, AL669842 Genomic DNA. Translation: CAI25691.1.
AL662894, AL606831, AL669842 Genomic DNA. Translation: CAI25793.1.
CH466601 Genomic DNA. Translation: EDL10446.1.
BC141294 mRNA. Translation: AAI41295.1.
U23505 mRNA. Translation: AAA87938.1.
CCDSiCCDS24864.1.
RefSeqiNP_032770.2. NM_008744.2.
UniGeneiMm.39095.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OVEX-ray2.64A23-457[»]
ProteinModelPortaliO09118.
SMRiO09118. Positions 39-453.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO09118. 2 interactions.
STRINGi10090.ENSMUSP00000021284.

PTM databases

PhosphoSiteiO09118.

Proteomic databases

MaxQBiO09118.
PRIDEiO09118.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021284; ENSMUSP00000021284; ENSMUSG00000020902.
ENSMUST00000108674; ENSMUSP00000104314; ENSMUSG00000020902.
GeneIDi18208.
KEGGimmu:18208.
UCSCiuc007jnm.1. mouse.

Organism-specific databases

CTDi9423.
MGIiMGI:105088. Ntn1.

Phylogenomic databases

eggNOGiNOG244580.
GeneTreeiENSGT00780000121873.
HOGENOMiHOG000286017.
HOVERGENiHBG006464.
InParanoidiO09118.
KOiK06843.
OMAiKMYNKPN.
OrthoDBiEOG7SR4KJ.
TreeFamiTF352481.

Enzyme and pathway databases

ReactomeiREACT_271763. DSCAM interactions.
REACT_293956. Netrin-1 signaling.
REACT_295531. DCC mediated attractive signaling.
REACT_336806. Role of second messengers in netrin-1 signaling.
REACT_337259. Netrin mediated repulsion signals.

Miscellaneous databases

NextBioi293590.
PROiO09118.
SOURCEiSearch...

Gene expression databases

BgeeiO09118.
CleanExiMM_NTN1.
ExpressionAtlasiO09118. baseline and differential.
GenevisibleiO09118. MM.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR002049. EGF_laminin.
IPR008979. Galactose-bd-like.
IPR008211. Laminin_N.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PfamiPF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF50242. SSF50242. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01248. EGF_LAM_1. 3 hits.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Netrin-1 is required for commissural axon guidance in the developing vertebrate nervous system."
    Serafini T., Colamarino S.A., Leonardo E.D., Wang H., Beddington R., Skarnes W.C., Tessier-Lavigne M.
    Cell 87:1001-1014(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Tissue: Embryonic brain.
  2. "Divergent properties of mouse netrins."
    Pueschel A.W.
    Mech. Dev. 83:65-75(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "Capturing genes encoding membrane and secreted proteins important for mouse development."
    Skarnes W.C., Moss J.E., Hurtley S.M., Beddington R.S.
    Proc. Natl. Acad. Sci. U.S.A. 92:6592-6596(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 352-398, SUBCELLULAR LOCATION.
  7. "DSCAM is a netrin receptor that collaborates with DCC in mediating turning responses to netrin-1."
    Ly A., Nikolaev A., Suresh G., Zheng Y., Tessier-Lavigne M., Stein E.
    Cell 133:1241-1254(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DSCAM.

Entry informationi

Entry nameiNET1_MOUSE
AccessioniPrimary (citable) accession number: O09118
Secondary accession number(s): B1ARR0, Q60832, Q9QY50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: July 27, 2011
Last modified: July 22, 2015
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.