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O09118

- NET1_MOUSE

UniProt

O09118 - NET1_MOUSE

Protein

Netrin-1

Gene

Ntn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. anterior/posterior axon guidance Source: MGI
    2. apoptotic process Source: UniProtKB-KW
    3. axon guidance Source: MGI
    4. axonogenesis Source: MGI
    5. establishment of nucleus localization Source: MGI
    6. inner ear morphogenesis Source: MGI
    7. mammary gland development Source: MGI
    8. mammary gland duct morphogenesis Source: MGI
    9. negative regulation of axon extension Source: MGI
    10. neuron migration Source: MGI
    11. positive regulation of axon extension Source: MGI
    12. positive regulation of cell proliferation Source: MGI
    13. regulation of cell migration Source: MGI
    14. single organismal cell-cell adhesion Source: MGI

    Keywords - Biological processi

    Apoptosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Netrin-1
    Gene namesi
    Name:Ntn1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:105088. Ntn1.

    Subcellular locationi

    Secretedextracellular spaceextracellular matrix 1 Publication

    GO - Cellular componenti

    1. basement membrane Source: MGI
    2. cell periphery Source: MGI
    3. cytoplasm Source: MGI
    4. extracellular region Source: Reactome

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 604580Netrin-1PRO_0000017083Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi119 ↔ 152By similarity
    Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi285 ↔ 294By similarity
    Disulfide bondi287 ↔ 304By similarity
    Disulfide bondi306 ↔ 315By similarity
    Disulfide bondi318 ↔ 338By similarity
    Disulfide bondi341 ↔ 350By similarity
    Disulfide bondi343 ↔ 368By similarity
    Disulfide bondi371 ↔ 380By similarity
    Disulfide bondi383 ↔ 401By similarity
    Disulfide bondi404 ↔ 416By similarity
    Disulfide bondi406 ↔ 423By similarity
    Glycosylationi417 – 4171N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi425 ↔ 434By similarity
    Disulfide bondi437 ↔ 451By similarity
    Disulfide bondi472 ↔ 544By similarity
    Disulfide bondi491 ↔ 601By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiO09118.

    PTM databases

    PhosphoSiteiO09118.

    Expressioni

    Tissue specificityi

    In the embryo, widely expressed in the developing nervous system and in mesodermal tissues.1 Publication

    Gene expression databases

    ArrayExpressiO09118.
    BgeeiO09118.
    CleanExiMM_NTN1.
    GenevestigatoriO09118.

    Interactioni

    Subunit structurei

    Binds to its receptors; DCC, UNC5A, UNC5B, UNC5C and probably UNC5D By similarity. Binds to its receptor; DSCAM.By similarity

    Protein-protein interaction databases

    IntActiO09118. 2 interactions.
    STRINGi10090.ENSMUSP00000021284.

    Structurei

    3D structure databases

    ProteinModelPortaliO09118.
    SMRiO09118. Positions 41-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini47 – 284238Laminin N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini285 – 34056Laminin EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini341 – 40363Laminin EGF-like 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini404 – 45350Laminin EGF-like 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini472 – 601130NTRPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi530 – 5323Cell attachment siteSequence Analysis

    Sequence similaritiesi

    Contains 3 laminin EGF-like domains.PROSITE-ProRule annotation
    Contains 1 laminin N-terminal domain.PROSITE-ProRule annotation
    Contains 1 NTR domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Laminin EGF-like domain, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG244580.
    GeneTreeiENSGT00750000117313.
    HOGENOMiHOG000286017.
    HOVERGENiHBG006464.
    InParanoidiB1ARR0.
    KOiK06843.
    OMAiDSYYYAV.
    OrthoDBiEOG7SR4KJ.
    TreeFamiTF352481.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    InterProiIPR013032. EGF-like_CS.
    IPR002049. EGF_laminin.
    IPR008979. Galactose-bd-like.
    IPR008211. Laminin_N.
    IPR001134. Netrin_domain.
    IPR018933. Netrin_module_non-TIMP.
    IPR008993. TIMP-like_OB-fold.
    [Graphical view]
    PfamiPF00053. Laminin_EGF. 3 hits.
    PF00055. Laminin_N. 1 hit.
    PF01759. NTR. 1 hit.
    [Graphical view]
    SMARTiSM00643. C345C. 1 hit.
    SM00180. EGF_Lam. 3 hits.
    SM00136. LamNT. 1 hit.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF50242. SSF50242. 1 hit.
    PROSITEiPS00022. EGF_1. 2 hits.
    PS01248. EGF_LAM_1. 3 hits.
    PS50027. EGF_LAM_2. 3 hits.
    PS51117. LAMININ_NTER. 1 hit.
    PS50189. NTR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O09118-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMRAVWEALA ALAAVACLVG AVRGGPGLSM FAGQAAQPDP CSDENGHPRR    50
    CIPDFVNAAF GKDVRVSSTC GRPPARYCVV SERGEERLRS CHLCNSSDPK 100
    KAHPPAFLTD LNNPHNLTCW QSENYLQFPH NVTLTLSLGK KFEVTYVSLQ 150
    FCSPRPESMA IYKSMDYGRT WVPFQFYSTQ CRKMYNRPHR APITKQNEQE 200
    AVCTDSHTDM RPLSGGLIAF STLDGRPSAH DFDNSPVLQD WVTATDIRVA 250
    FSRLHTFGDE NEDDSELARD SYYYAVSDLQ VGGRCKCNGH AARCVRDRDD 300
    SLVCDCRHNT AGPECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC 350
    RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA 400
    CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP 450
    CIKIPVAPPT TAASSVEEPE DCDSYCKASK GKLKMNMKKY CRKDYAVQIH 500
    ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIACKCPKIK 550
    PLKKYLLLGN AEDSPDQSGI VADKSSLVIQ WRDTWARRLR KFQQREKKGK 600
    CKKA 604
    Length:604
    Mass (Da):67,810
    Last modified:July 27, 2011 - v3
    Checksum:i135120DB6F0482F3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241Missing in AAC52971. (PubMed:8978605)Curated
    Sequence conflicti88 – 881L → V in AAC52971. (PubMed:8978605)Curated
    Sequence conflicti88 – 881L → V in AAD28602. (PubMed:10381568)Curated
    Sequence conflicti466 – 4661V → A in AAD28602. (PubMed:10381568)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U65418 mRNA. Translation: AAC52971.1.
    AF128865 mRNA. Translation: AAD28602.1.
    AL669842, AL606831, AL662894 Genomic DNA. Translation: CAI24775.1.
    AL606831, AL662894, AL669842 Genomic DNA. Translation: CAI25691.1.
    AL662894, AL606831, AL669842 Genomic DNA. Translation: CAI25793.1.
    CH466601 Genomic DNA. Translation: EDL10446.1.
    BC141294 mRNA. Translation: AAI41295.1.
    U23505 mRNA. Translation: AAA87938.1.
    CCDSiCCDS24864.1.
    RefSeqiNP_032770.2. NM_008744.2.
    UniGeneiMm.39095.

    Genome annotation databases

    EnsembliENSMUST00000021284; ENSMUSP00000021284; ENSMUSG00000020902.
    ENSMUST00000108674; ENSMUSP00000104314; ENSMUSG00000020902.
    GeneIDi18208.
    KEGGimmu:18208.
    UCSCiuc007jnm.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U65418 mRNA. Translation: AAC52971.1 .
    AF128865 mRNA. Translation: AAD28602.1 .
    AL669842 , AL606831 , AL662894 Genomic DNA. Translation: CAI24775.1 .
    AL606831 , AL662894 , AL669842 Genomic DNA. Translation: CAI25691.1 .
    AL662894 , AL606831 , AL669842 Genomic DNA. Translation: CAI25793.1 .
    CH466601 Genomic DNA. Translation: EDL10446.1 .
    BC141294 mRNA. Translation: AAI41295.1 .
    U23505 mRNA. Translation: AAA87938.1 .
    CCDSi CCDS24864.1.
    RefSeqi NP_032770.2. NM_008744.2.
    UniGenei Mm.39095.

    3D structure databases

    ProteinModelPortali O09118.
    SMRi O09118. Positions 41-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O09118. 2 interactions.
    STRINGi 10090.ENSMUSP00000021284.

    PTM databases

    PhosphoSitei O09118.

    Proteomic databases

    PRIDEi O09118.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021284 ; ENSMUSP00000021284 ; ENSMUSG00000020902 .
    ENSMUST00000108674 ; ENSMUSP00000104314 ; ENSMUSG00000020902 .
    GeneIDi 18208.
    KEGGi mmu:18208.
    UCSCi uc007jnm.1. mouse.

    Organism-specific databases

    CTDi 9423.
    MGIi MGI:105088. Ntn1.

    Phylogenomic databases

    eggNOGi NOG244580.
    GeneTreei ENSGT00750000117313.
    HOGENOMi HOG000286017.
    HOVERGENi HBG006464.
    InParanoidi B1ARR0.
    KOi K06843.
    OMAi DSYYYAV.
    OrthoDBi EOG7SR4KJ.
    TreeFami TF352481.

    Miscellaneous databases

    NextBioi 293590.
    PROi O09118.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O09118.
    Bgeei O09118.
    CleanExi MM_NTN1.
    Genevestigatori O09118.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    InterProi IPR013032. EGF-like_CS.
    IPR002049. EGF_laminin.
    IPR008979. Galactose-bd-like.
    IPR008211. Laminin_N.
    IPR001134. Netrin_domain.
    IPR018933. Netrin_module_non-TIMP.
    IPR008993. TIMP-like_OB-fold.
    [Graphical view ]
    Pfami PF00053. Laminin_EGF. 3 hits.
    PF00055. Laminin_N. 1 hit.
    PF01759. NTR. 1 hit.
    [Graphical view ]
    SMARTi SM00643. C345C. 1 hit.
    SM00180. EGF_Lam. 3 hits.
    SM00136. LamNT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF50242. SSF50242. 1 hit.
    PROSITEi PS00022. EGF_1. 2 hits.
    PS01248. EGF_LAM_1. 3 hits.
    PS50027. EGF_LAM_2. 3 hits.
    PS51117. LAMININ_NTER. 1 hit.
    PS50189. NTR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Netrin-1 is required for commissural axon guidance in the developing vertebrate nervous system."
      Serafini T., Colamarino S.A., Leonardo E.D., Wang H., Beddington R., Skarnes W.C., Tessier-Lavigne M.
      Cell 87:1001-1014(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Tissue: Embryonic brain.
    2. "Divergent properties of mouse netrins."
      Pueschel A.W.
      Mech. Dev. 83:65-75(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. "Capturing genes encoding membrane and secreted proteins important for mouse development."
      Skarnes W.C., Moss J.E., Hurtley S.M., Beddington R.S.
      Proc. Natl. Acad. Sci. U.S.A. 92:6592-6596(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 352-398, SUBCELLULAR LOCATION.
    7. "DSCAM is a netrin receptor that collaborates with DCC in mediating turning responses to netrin-1."
      Ly A., Nikolaev A., Suresh G., Zheng Y., Tessier-Lavigne M., Stein E.
      Cell 133:1241-1254(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DSCAM.

    Entry informationi

    Entry nameiNET1_MOUSE
    AccessioniPrimary (citable) accession number: O09118
    Secondary accession number(s): B1ARR0, Q60832, Q9QY50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 130 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3