Netrin-1 precursor - Mus musculus (Mouse)

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O09118 (NET1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 117. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Netrin-1

Gene names

Name:

Ntn1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis By similarity. Ref.1 Ref.2
Subunit structure	Binds to its receptors; DCC, UNC5A, UNC5B, UNC5C and probably UNC5D By similarity. Binds to its receptor; DSCAM. Ref.7
Subcellular location	Secreted › extracellular space › extracellular matrix Ref.6.
Tissue specificity	In the embryo, widely expressed in the developing nervous system and in mesodermal tissues. Ref.2
Sequence similarities	Contains 3 laminin EGF-like domains. Contains 1 laminin N-terminal domain. Contains 1 NTR domain.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Extracellular matrix Secreted
Domain	Laminin EGF-like domain Repeat Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anterior/posterior axon guidance Inferred from mutant phenotype PubMed 18479186. Source: MGI apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cell-cell adhesion Inferred from genetic interaction PubMed 16439476. Source: MGI establishment of nucleus localization Inferred from direct assay PubMed 12051827. Source: MGI inner ear morphogenesis Inferred from mutant phenotype PubMed 17616930. Source: MGI mammary gland duct morphogenesis Inferred from genetic interaction PubMed 16439476. Source: MGI negative regulation of axon extension Inferred from direct assay PubMed 10704383. Source: MGI neuron migration Inferred from mutant phenotype PubMed 14750959 PubMed 16267219. Source: MGI positive regulation of axon extension Inferred from direct assay PubMed 10704383. Source: MGI positive regulation of cell proliferation Inferred from mutant phenotype PubMed 16481423. Source: MGI regulation of cell migration Inferred from direct assay PubMed 10704383. Source: MGI
Cellular_component	basement membrane Inferred from direct assay PubMed 18757743. Source: MGI cell periphery Inferred from sequence orthology PubMed 16455951. Source: MGI cytoplasm Inferred from direct assay PubMed 18425773. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

Potential

Chain

25 – 604

580

Netrin-1

PRO_0000017083

Regions

Domain

47 – 284

238

Laminin N-terminal

Domain

285 – 340

Laminin EGF-like 1

Domain

341 – 403

Laminin EGF-like 2

Domain

404 – 453

Laminin EGF-like 3

Domain

472 – 601

130

NTR

Motif

530 – 532

Cell attachment site Potential

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

116

N-linked (GlcNAc...) Potential

Glycosylation

131

N-linked (GlcNAc...) Potential

Glycosylation

417

N-linked (GlcNAc...) Potential

Disulfide bond

119 ↔ 152

By similarity

Disulfide bond

285 ↔ 294

By similarity

Disulfide bond

287 ↔ 304

By similarity

Disulfide bond

306 ↔ 315

By similarity

Disulfide bond

318 ↔ 338

By similarity

Disulfide bond

341 ↔ 350

By similarity

Disulfide bond

343 ↔ 368

By similarity

Disulfide bond

371 ↔ 380

By similarity

Disulfide bond

383 ↔ 401

By similarity

Disulfide bond

404 ↔ 416

By similarity

Disulfide bond

406 ↔ 423

By similarity

Disulfide bond

425 ↔ 434

By similarity

Disulfide bond

437 ↔ 451

By similarity

Disulfide bond

472 ↔ 544

By similarity

Disulfide bond

491 ↔ 601

By similarity

Experimental info

Sequence conflict

Missing in AAC52971. Ref.1

Sequence conflict

L → V in AAC52971. Ref.1

Sequence conflict

L → V in AAD28602. Ref.2

Sequence conflict

466

V → A in AAD28602. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

O09118 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 135120DB6F0482F3
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FASTA

604

67,810

        10         20         30         40         50         60 
MMRAVWEALA ALAAVACLVG AVRGGPGLSM FAGQAAQPDP CSDENGHPRR CIPDFVNAAF 

        70         80         90        100        110        120 
GKDVRVSSTC GRPPARYCVV SERGEERLRS CHLCNSSDPK KAHPPAFLTD LNNPHNLTCW 

       130        140        150        160        170        180 
QSENYLQFPH NVTLTLSLGK KFEVTYVSLQ FCSPRPESMA IYKSMDYGRT WVPFQFYSTQ 

       190        200        210        220        230        240 
CRKMYNRPHR APITKQNEQE AVCTDSHTDM RPLSGGLIAF STLDGRPSAH DFDNSPVLQD 

       250        260        270        280        290        300 
WVTATDIRVA FSRLHTFGDE NEDDSELARD SYYYAVSDLQ VGGRCKCNGH AARCVRDRDD 

       310        320        330        340        350        360 
SLVCDCRHNT AGPECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS 

       370        380        390        400        410        420 
GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA CKACDCHPVG AAGKTCNQTT 

       430        440        450        460        470        480 
GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSVEEPE DCDSYCKASK 

       490        500        510        520        530        540 
GKLKMNMKKY CRKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR 

       550        560        570        580        590        600 
DIACKCPKIK PLKKYLLLGN AEDSPDQSGI VADKSSLVIQ WRDTWARRLR KFQQREKKGK 


CKKA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Netrin-1 is required for commissural axon guidance in the developing vertebrate nervous system." Serafini T., Colamarino S.A., Leonardo E.D., Wang H., Beddington R., Skarnes W.C., Tessier-Lavigne M. Cell 87:1001-1014(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Embryonic brain.
[2]	"Divergent properties of mouse netrins." Pueschel A.W. Mech. Dev. 83:65-75(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Capturing genes encoding membrane and secreted proteins important for mouse development." Skarnes W.C., Moss J.E., Hurtley S.M., Beddington R.S. Proc. Natl. Acad. Sci. U.S.A. 92:6592-6596(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 352-398, SUBCELLULAR LOCATION.
[7]	"DSCAM is a netrin receptor that collaborates with DCC in mediating turning responses to netrin-1." Ly A., Nikolaev A., Suresh G., Zheng Y., Tessier-Lavigne M., Stein E. Cell 133:1241-1254(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DSCAM.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U65418 mRNA. Translation: AAC52971.1. AF128865 mRNA. Translation: AAD28602.1. AL669842, AL606831, AL662894 Genomic DNA. Translation: CAI24775.1. AL606831, AL662894, AL669842 Genomic DNA. Translation: CAI25691.1. AL662894, AL606831, AL669842 Genomic DNA. Translation: CAI25793.1. CH466601 Genomic DNA. Translation: EDL10446.1. BC141294 mRNA. Translation: AAI41295.1. U23505 mRNA. Translation: AAA87938.1.
IPI	IPI00114257.
RefSeq	NP_032770.2. NM_008744.2.
UniGene	Mm.39095.
3D structure databases
ProteinModelPortal	O09118.
SMR	O09118. Positions 41-446.
ModBase	Search...
Protein-protein interaction databases
IntAct	O09118. 2 interactions.
STRING	10090.ENSMUSP00000021284.
PTM databases
PhosphoSite	O09118.
Proteomic databases
PRIDE	O09118.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000021284; ENSMUSP00000021284; ENSMUSG00000020902. ENSMUST00000108674; ENSMUSP00000104314; ENSMUSG00000020902.
GeneID	18208.
KEGG	mmu:18208.
UCSC	uc007jnm.1. mouse.
Organism-specific databases
CTD	9423.
MGI	MGI:105088. Ntn1.
Phylogenomic databases
eggNOG	NOG244580.
GeneTree	ENSGT00690000102103.
HOGENOM	HOG000286017.
HOVERGEN	HBG006464.
InParanoid	B1ARR0.
KO	K06843.
OMA	NCHTCDA.
OrthoDB	EOG4H19V9.
Enzyme and pathway databases
Reactome	REACT_127416. Developmental Biology. REACT_137402. Cell-Cell communication.
Gene expression databases
ArrayExpress	O09118.
Bgee	O09118.
CleanEx	MM_NTN1.
Genevestigator	O09118.
GermOnline	ENSMUSG00000020902. Mus musculus.
Family and domain databases
Gene3D	2.60.120.260. 1 hit.
InterPro	IPR013032. EGF-like_CS. IPR002049. EGF_laminin. IPR008979. Galactose-bd-like. IPR008211. Laminin_N. IPR001134. Netrin_domain. IPR018933. Netrin_module_non-TIMP. IPR008993. TIMP-like_OB-fold. [Graphical view]
Pfam	PF00053. Laminin_EGF. 3 hits. PF00055. Laminin_N. 1 hit. PF01759. NTR. 1 hit. [Graphical view]
SMART	SM00643. C345C. 1 hit. SM00180. EGF_Lam. 3 hits. SM00136. LamNT. 1 hit. [Graphical view]
SUPFAM	SSF50242. TIMP_like. 1 hit.
PROSITE	PS00022. EGF_1. 2 hits. PS01186. EGF_2. False negative. PS01248. EGF_LAM_1. 3 hits. PS50027. EGF_LAM_2. 3 hits. PS51117. LAMININ_NTER. 1 hit. PS50189. NTR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	293590.
SOURCE	Search...

Entry information

Entry name

NET1_MOUSE

Accession

Primary (citable) accession number: O09118
Secondary accession number(s): B1ARR0, Q60832, Q9QY50

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	July 27, 2011
Last modified:	May 29, 2013
This is version 117 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents