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Protein

Dual specificity protein phosphatase 8

Gene

Dusp8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This protein shows both activity toward tyrosine-protein phosphate as well as with serine/threonine-protein phosphate.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei246 – 2461Phosphocysteine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. MAP kinase tyrosine/serine/threonine phosphatase activity Source: InterPro
  2. protein tyrosine/serine/threonine phosphatase activity Source: GO_Central
  3. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein dephosphorylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 8 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Neuronal tyrosine threonine phosphatase 1
Gene namesi
Name:Dusp8
Synonyms:Nttp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:106626. Dusp8.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 663663Dual specificity protein phosphatase 8PRO_0000094811Add
BLAST

Proteomic databases

MaxQBiO09112.
PRIDEiO09112.

PTM databases

PhosphoSiteiO09112.

Expressioni

Tissue specificityi

Expressed predominantly in brain and lung.1 Publication

Gene expression databases

BgeeiO09112.
CleanExiMM_DUSP8.
ExpressionAtlasiO09112. baseline and differential.
GenevestigatoriO09112.

Structurei

3D structure databases

ProteinModelPortaliO09112.
SMRiO09112. Positions 14-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 138116RhodanesePROSITE-ProRule annotationAdd
BLAST
Domaini162 – 432271Tyrosine-protein phosphataseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi311 – 552242Pro-richAdd
BLAST
Compositional biasi452 – 4598Poly-Arg
Compositional biasi555 – 5584Poly-Ser
Compositional biasi559 – 57618Poly-GlyAdd
BLAST
Compositional biasi577 – 60024Poly-SerAdd
BLAST

Sequence similaritiesi

Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00760000118902.
HOVERGENiHBG005541.
InParanoidiO09112.
KOiK04459.
OMAiDGRHSWH.
OrthoDBiEOG747PJD.
TreeFamiTF105122.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR01764. MAPKPHPHTASE.
SMARTiSM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O09112-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGDRLPRKV MDAKKLASLL RGGPGGPLVI DSRSFVEYNS CHVLSSVNIC
60 70 80 90 100
CSKLVKRRLQ QGKVTIAELI QPATRSQVDA TEPQDVVVYD QSTRDASVLA
110 120 130 140 150
ADSFLSILLS KLDGCFDSVA ILTGGFATFS SCFPGLCEGK PATLPSMSLS
160 170 180 190 200
QPCLPVPSVG LTRILPHLYL GSQKDVLNKD LMTQNGISYV LNASNSCPKP
210 220 230 240 250
DFICESRFMR IPINDNYCEK LLPWLDKSIE FIDKAKLSSC QVIVHCLAGI
260 270 280 290 300
SRSATIAIAY IMKTMGMSSD DAYRFVKDRR PSISPNFNFL GQLLEYERSL
310 320 330 340 350
KLLAALQTDG PHLGTPEPLM GPAAGIPLPR LPPSTSESAA TGSEAATAAR
360 370 380 390 400
EGSPSAGGDA PIPSTAPATS ALQQGLRGLH LSSDRLQDTN RLKRSFSLDI
410 420 430 440 450
KSAYAPSRRP DFPGPPDPGE APKLCKLDSP SGGTLGLPSP SPDSPDSVPE
460 470 480 490 500
CRPRPRRRRP PASSPARSPA HGLGLNFGDT ARQTPRHGLS ALSAPGLPGP
510 520 530 540 550
GQPAGPGGWV PPLDSPGTPS PDGPWCFSPE GAQGPGAVFS AFGRVSAGAP
560 570 580 590 600
GPGNSSSSGG GGGGGGGGGG GGGGGGSSSS NSSSSSSSSS SSSSSSSSSS
610 620 630 640 650
DLRRRDVRTG WPEEPAADAQ FKRRSCQMEF EEGMVEGRAR GEELAALGKQ
660
TSFSGSVEVI EVS
Length:663
Mass (Da):68,847
Last modified:July 1, 1997 - v1
Checksum:i416F429A12C1FA7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95518 mRNA. Translation: CAA64772.1.
CCDSiCCDS22024.1.
RefSeqiNP_032774.1. NM_008748.3.
XP_006508572.1. XM_006508509.1.
UniGeneiMm.39725.

Genome annotation databases

EnsembliENSMUST00000039926; ENSMUSP00000049414; ENSMUSG00000037887.
GeneIDi18218.
KEGGimmu:18218.
UCSCiuc009kmo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95518 mRNA. Translation: CAA64772.1.
CCDSiCCDS22024.1.
RefSeqiNP_032774.1. NM_008748.3.
XP_006508572.1. XM_006508509.1.
UniGeneiMm.39725.

3D structure databases

ProteinModelPortaliO09112.
SMRiO09112. Positions 14-309.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiO09112.

Proteomic databases

MaxQBiO09112.
PRIDEiO09112.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039926; ENSMUSP00000049414; ENSMUSG00000037887.
GeneIDi18218.
KEGGimmu:18218.
UCSCiuc009kmo.2. mouse.

Organism-specific databases

CTDi1850.
MGIiMGI:106626. Dusp8.

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00760000118902.
HOVERGENiHBG005541.
InParanoidiO09112.
KOiK04459.
OMAiDGRHSWH.
OrthoDBiEOG747PJD.
TreeFamiTF105122.

Miscellaneous databases

NextBioi293632.
PROiO09112.
SOURCEiSearch...

Gene expression databases

BgeeiO09112.
CleanExiMM_DUSP8.
ExpressionAtlasiO09112. baseline and differential.
GenevestigatoriO09112.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR01764. MAPKPHPHTASE.
SMARTiSM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A member of the MAP kinase phosphatase gene family in mouse containing a complex trinucleotide repeat in the coding region."
    Theodosiou A.M., Rodrigues N.R., Nesbit M.A., Ambrose H.J., Paterson H., McLellan-Arnold E., Boyd Y., Leversha M.A., Owen N., Blake D.J., Ashworth A., Davies K.E.
    Hum. Mol. Genet. 5:675-684(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain.

Entry informationi

Entry nameiDUS8_MOUSE
AccessioniPrimary (citable) accession number: O09112
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 1, 1997
Last modified: February 4, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.