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Protein

Insulin-like 3

Gene

Insl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • in utero embryonic development Source: MGI
  • male gonad development Source: MGI
  • positive regulation of epithelial cell migration Source: MGI
  • positive regulation of wound healing Source: MGI
  • regulation of male gonad development Source: CACAO

Keywordsi

Molecular functionHormone

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like 3
Alternative name(s):
Leydig insulin-like peptide
Short name:
Ley-I-L
Relaxin-like factor
Cleaved into the following 2 chains:
Gene namesi
Name:Insl3
Synonyms:Rlf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:108427. Insl3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Male mice exhibit bilateral abdominal cryptorchidism due to alteration of gubernaculum development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_0000016144? – 94C peptide likeSequence analysis
Signal peptidei1 – 15Sequence analysisAdd BLAST15
PeptideiPRO_000001614316 – ?Insulin-like 3 B chain
PeptideiPRO_000001614597 – 122Insulin-like 3 A chainAdd BLAST26

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi29 ↔ 107Interchain (between B and A chains)By similarity
Disulfide bondi41 ↔ 120Interchain (between B and A chains)By similarity
Disulfide bondi106 ↔ 111By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiO09107.
PRIDEiO09107.

Expressioni

Tissue specificityi

Expressed exclusively in Leydig cells of the testis.

Gene expression databases

CleanExiMM_INSL3.
MM_RLF.

Interactioni

Subunit structurei

Heterodimer of a B chain and an A chain linked by two disulfide bonds.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034261.

Structurei

3D structure databases

ProteinModelPortaliO09107.
SMRiO09107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J0IS. Eukaryota.
ENOG411140J. LUCA.
HOGENOMiHOG000113077.
HOVERGENiHBG052133.
InParanoidiO09107.
PhylomeDBiO09107.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiView protein in InterPro
IPR016179. Insulin-like.
IPR036438. Insulin-like_sf.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
PfamiView protein in Pfam
PF00049. Insulin. 1 hit.
PRINTSiPR00276. INSULINFAMLY.
SMARTiView protein in SMART
SM00078. IlGF. 1 hit.
SUPFAMiSSF56994. SSF56994. 2 hits.
PROSITEiView protein in PROSITE
PS00262. INSULIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O09107-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAPLLLMLL ALGSALRSPQ PPEARAKLCG HHLVRTLVRV CGGPRWSPEA
60 70 80 90 100
TQPVETRDRE LLQWLEQRHL LHALVADVDP ALDPQLPRQA SQRQRRSAAT
110 120
NAVHRCCLTG CTQQDLLGLC PH
Length:122
Mass (Da):13,586
Last modified:November 16, 2001 - v2
Checksum:i10783AB4896CF103
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21P → T in CAA64861 (Ref. 1) Curated1
Sequence conflicti54 – 58VETRD → CGDPG in CAA64861 (Ref. 1) Curated5
Sequence conflicti73A → S in CAA64861 (Ref. 1) Curated1
Sequence conflicti92 – 96QRQRR → HARG in CAA64861 (Ref. 1) Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95603 Genomic DNA. Translation: CAA64861.1.
S82815 mRNA. Translation: AAB39365.1.
AF136524 Genomic DNA. Translation: AAD24585.1.
CCDSiCCDS22404.1.
UniGeneiMm.476857.

Similar proteinsi

Entry informationi

Entry nameiINSL3_MOUSE
AccessioniPrimary (citable) accession number: O09107
Secondary accession number(s): P97744
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 16, 2001
Last modified: October 25, 2017
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families