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Protein

Phosphatidylinositol-glycan biosynthesis class F protein

Gene

Pigf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.1 Publication

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

  • phosphotransferase activity, for other substituted phosphate groups Source: MGI

GO - Biological processi

  • GPI anchor biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-162710. Synthesis of glycosylphosphatidylinositol (GPI).
UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol-glycan biosynthesis class F protein
Short name:
PIG-F
Gene namesi
Name:Pigf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:99462. Pigf.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei11 – 3121HelicalSequence analysisAdd
BLAST
Transmembranei42 – 6221HelicalSequence analysisAdd
BLAST
Transmembranei86 – 10621HelicalSequence analysisAdd
BLAST
Transmembranei113 – 13321HelicalSequence analysisAdd
BLAST
Transmembranei155 – 17521HelicalSequence analysisAdd
BLAST
Transmembranei189 – 20921HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 219219Phosphatidylinositol-glycan biosynthesis class F proteinPRO_0000191760Add
BLAST

Proteomic databases

EPDiO09101.
PaxDbiO09101.
PRIDEiO09101.

Expressioni

Gene expression databases

BgeeiO09101.
GenevisibleiO09101. MM.

Interactioni

Subunit structurei

Forms a complex with PIGG and PIGO. PIGF is required to stabilize PIGG and PIGO.1 Publication

Protein-protein interaction databases

BioGridi202156. 2 interactions.
STRINGi10090.ENSMUSP00000024957.

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGF family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3144. Eukaryota.
ENOG4111B1J. LUCA.
GeneTreeiENSGT00390000016617.
HOGENOMiHOG000082526.
HOVERGENiHBG031820.
InParanoidiO09101.
KOiK05287.
OMAiFFHVIFV.
OrthoDBiEOG76HQ2X.
PhylomeDBiO09101.
TreeFamiTF323878.

Family and domain databases

InterProiIPR009580. GPI_biosynthesis_protein_Pig-F.
[Graphical view]
PfamiPF06699. PIG-F. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O09101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDTDIKRLL YTNLLCVFSI FLSIFIPSFF VDNFSVLEAH LTWLCICSAS
60 70 80 90 100
VTTVNLLSYL VVKPNVSSKR SSLSHKVTRA LKCCVCFLMS CFLLHIIFVL
110 120 130 140 150
YGAPLIELVL ETFLFAVVLS TFTTVPCLCL LGPNLKAWLR VFSRNGVTSI
160 170 180 190 200
WENSLQITTI SSFTGAWLGA FPIPLDWERP WQVWPISCTL GATFGYVAGL
210
VISPLWIYWN RKQLTYKNN
Length:219
Mass (Da):24,825
Last modified:July 1, 1997 - v1
Checksum:iBD78A77E69C5BC1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50264 mRNA. Translation: BAA08818.1.
CCDSiCCDS29011.1.
RefSeqiNP_032864.1. NM_008838.1.
XP_006523894.1. XM_006523831.2.
UniGeneiMm.219685.

Genome annotation databases

EnsembliENSMUST00000024957; ENSMUSP00000024957; ENSMUSG00000024145.
GeneIDi18701.
KEGGimmu:18701.
UCSCiuc008dun.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50264 mRNA. Translation: BAA08818.1.
CCDSiCCDS29011.1.
RefSeqiNP_032864.1. NM_008838.1.
XP_006523894.1. XM_006523831.2.
UniGeneiMm.219685.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202156. 2 interactions.
STRINGi10090.ENSMUSP00000024957.

Proteomic databases

EPDiO09101.
PaxDbiO09101.
PRIDEiO09101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024957; ENSMUSP00000024957; ENSMUSG00000024145.
GeneIDi18701.
KEGGimmu:18701.
UCSCiuc008dun.1. mouse.

Organism-specific databases

CTDi5281.
MGIiMGI:99462. Pigf.

Phylogenomic databases

eggNOGiKOG3144. Eukaryota.
ENOG4111B1J. LUCA.
GeneTreeiENSGT00390000016617.
HOGENOMiHOG000082526.
HOVERGENiHBG031820.
InParanoidiO09101.
KOiK05287.
OMAiFFHVIFV.
OrthoDBiEOG76HQ2X.
PhylomeDBiO09101.
TreeFamiTF323878.

Enzyme and pathway databases

UniPathwayiUPA00196.
ReactomeiR-MMU-162710. Synthesis of glycosylphosphatidylinositol (GPI).

Miscellaneous databases

NextBioi294751.
PROiO09101.
SOURCEiSearch...

Gene expression databases

BgeeiO09101.
GenevisibleiO09101. MM.

Family and domain databases

InterProiIPR009580. GPI_biosynthesis_protein_Pig-F.
[Graphical view]
PfamiPF06699. PIG-F. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of the murine GPI anchor synthesis gene Pigf, a homologue of the human PIGF gene."
    Ohishi K., Kurimoto Y., Inoue N., Endo Y., Takeda J., Kinoshita T.
    Genomics 34:340-346(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  2. "Requirement of PIG-F and PIG-O for transferring phosphoethanolamine to the third mannose in glycosylphosphatidylinositol."
    Hong Y., Maeda Y., Watanabe R., Inoue N., Ohishi K., Kinoshita T.
    J. Biol. Chem. 275:20911-20919(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
    Tissue: Testis.

Entry informationi

Entry nameiPIGF_MOUSE
AccessioniPrimary (citable) accession number: O09101
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: July 1, 1997
Last modified: March 16, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.