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Protein

Phosphatidylinositol-glycan biosynthesis class F protein

Gene

Pigf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.1 Publication

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • GPI anchor biosynthetic process Source: UniProtKB
  • preassembly of GPI anchor in ER membrane Source: Reactome
Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-162710. Synthesis of glycosylphosphatidylinositol (GPI).
UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol-glycan biosynthesis class F protein
Short name:
PIG-F
Gene namesi
Name:Pigf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:99462. Pigf.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei11 – 31HelicalSequence analysisAdd BLAST21
Transmembranei42 – 62HelicalSequence analysisAdd BLAST21
Transmembranei86 – 106HelicalSequence analysisAdd BLAST21
Transmembranei113 – 133HelicalSequence analysisAdd BLAST21
Transmembranei155 – 175HelicalSequence analysisAdd BLAST21
Transmembranei189 – 209HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001917601 – 219Phosphatidylinositol-glycan biosynthesis class F proteinAdd BLAST219

Proteomic databases

EPDiO09101.
PaxDbiO09101.
PRIDEiO09101.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024145.
GenevisibleiO09101. MM.

Interactioni

Subunit structurei

Forms a complex with PIGG and PIGO. PIGF is required to stabilize PIGG and PIGO.1 Publication

Protein-protein interaction databases

BioGridi202156. 2 interactors.
STRINGi10090.ENSMUSP00000024957.

Structurei

3D structure databases

ProteinModelPortaliO09101.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGF family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3144. Eukaryota.
ENOG4111B1J. LUCA.
GeneTreeiENSGT00390000016617.
HOGENOMiHOG000082526.
HOVERGENiHBG031820.
InParanoidiO09101.
KOiK05287.
OMAiFFHVIFV.
OrthoDBiEOG091G0HMT.
PhylomeDBiO09101.
TreeFamiTF323878.

Family and domain databases

InterProiIPR009580. GPI_biosynthesis_protein_Pig-F.
[Graphical view]
PfamiPF06699. PIG-F. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O09101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDTDIKRLL YTNLLCVFSI FLSIFIPSFF VDNFSVLEAH LTWLCICSAS
60 70 80 90 100
VTTVNLLSYL VVKPNVSSKR SSLSHKVTRA LKCCVCFLMS CFLLHIIFVL
110 120 130 140 150
YGAPLIELVL ETFLFAVVLS TFTTVPCLCL LGPNLKAWLR VFSRNGVTSI
160 170 180 190 200
WENSLQITTI SSFTGAWLGA FPIPLDWERP WQVWPISCTL GATFGYVAGL
210
VISPLWIYWN RKQLTYKNN
Length:219
Mass (Da):24,825
Last modified:July 1, 1997 - v1
Checksum:iBD78A77E69C5BC1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50264 mRNA. Translation: BAA08818.1.
CCDSiCCDS29011.1.
RefSeqiNP_032864.1. NM_008838.1.
XP_006523894.1. XM_006523831.2.
UniGeneiMm.219685.

Genome annotation databases

EnsembliENSMUST00000024957; ENSMUSP00000024957; ENSMUSG00000024145.
GeneIDi18701.
KEGGimmu:18701.
UCSCiuc008dun.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50264 mRNA. Translation: BAA08818.1.
CCDSiCCDS29011.1.
RefSeqiNP_032864.1. NM_008838.1.
XP_006523894.1. XM_006523831.2.
UniGeneiMm.219685.

3D structure databases

ProteinModelPortaliO09101.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202156. 2 interactors.
STRINGi10090.ENSMUSP00000024957.

Proteomic databases

EPDiO09101.
PaxDbiO09101.
PRIDEiO09101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024957; ENSMUSP00000024957; ENSMUSG00000024145.
GeneIDi18701.
KEGGimmu:18701.
UCSCiuc008dun.1. mouse.

Organism-specific databases

CTDi5281.
MGIiMGI:99462. Pigf.

Phylogenomic databases

eggNOGiKOG3144. Eukaryota.
ENOG4111B1J. LUCA.
GeneTreeiENSGT00390000016617.
HOGENOMiHOG000082526.
HOVERGENiHBG031820.
InParanoidiO09101.
KOiK05287.
OMAiFFHVIFV.
OrthoDBiEOG091G0HMT.
PhylomeDBiO09101.
TreeFamiTF323878.

Enzyme and pathway databases

UniPathwayiUPA00196.
ReactomeiR-MMU-162710. Synthesis of glycosylphosphatidylinositol (GPI).

Miscellaneous databases

PROiO09101.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024145.
GenevisibleiO09101. MM.

Family and domain databases

InterProiIPR009580. GPI_biosynthesis_protein_Pig-F.
[Graphical view]
PfamiPF06699. PIG-F. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIGF_MOUSE
AccessioniPrimary (citable) accession number: O09101
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.