Reviewed, UniProtKB/Swiss-Prot O09047 (C3AR_MOUSE)

Names and origin

Protein names

Recommended name:
    C3a anaphylatoxin chemotactic receptor
      Short name=C3a-R
      Short name=C3AR
Alternative name(s):
    Complement component 3a receptor 1

Gene names

Name:	C3ar1
Synonyms:	C3r1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Detected in varying levels in all tissues examined except the spleen. Especially abundant in heart and lung.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Sulfation
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	C3a anaphylatoxin receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	C3a anaphylatoxin chemotactic receptor		PRO_0000069204
Regions
	Topological domain	1 – 23	23	Extracellular Potential
	Transmembrane	24 – 46	23	1 Potential
	Topological domain	47 – 57	11	Cytoplasmic Potential
	Transmembrane	58 – 80	23	2 Potential
	Topological domain	81 – 96	16	Extracellular Potential
	Transmembrane	97 – 118	22	3 Potential
	Topological domain	119 – 139	21	Cytoplasmic Potential
	Transmembrane	140 – 160	21	4 Potential
	Topological domain	161 – 333	173	Extracellular Potential
	Transmembrane	334 – 353	20	5 Potential
	Topological domain	354 – 370	17	Cytoplasmic Potential
	Transmembrane	371 – 393	23	6 Potential
	Topological domain	394 – 410	17	Extracellular Potential
	Transmembrane	411 – 431	21	7 Potential
	Topological domain	432 – 477	46	Cytoplasmic Potential
Amino acid modifications
	Modified residue	174	1	Sulfotyrosine By similarity
	Modified residue	184	1	Sulfotyrosine By similarity
	Modified residue	312	1	Sulfotyrosine By similarity
	Glycosylation	9	1	N-linked (GlcNAc...) Potential
	Glycosylation	168	1	N-linked (GlcNAc...) Potential
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	201	1	N-linked (GlcNAc...) Potential
	Disulfide bond	95 ↔ 172		By similarity
Experimental info
	Sequence conflict	173	1	R → K in AAB71814. Ref.2
	Sequence conflict	195	1	E → K in AAB71814. Ref.2
	Sequence conflict	243	1	D → N in AAB71814. Ref.2
	Sequence conflict	276	1	D → N in AAB71814. Ref.2
	Sequence conflict	380 – 382	3	FIC → LS in AAB71814. Ref.2
	Sequence conflict	391	1	V → I in AAB71814. Ref.2
	Sequence conflict	460	1	N → S in AAB71814. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O09047-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 9873F464A1520984	FASTA	477	53,576
	10 20 30 40 50 60 MESFDADTNS TDLHSRPLFQ PQDIASMVIL GLTCLLGLLG NGLVLWVAGV KMKTTVNTVW 70 80 90 100 110 120 FLHLTLADFL CCLSLPFSLA HLILQGHWPY GLFLCKLIPS IIILNMFASV FLLTAISLDR 130 140 150 160 170 180 CLIVHKPIWC QNHRNVRTAF AICGCVWVVA FVMCVPVFVY RDLFIMDNRS ICRYNFDSSR 190 200 210 220 230 240 SYDYWDYVYK LSLPESNSTD NSTAQLTGHM NDRSAPSSVQ ARDYFWTVTT ALQSQPFLTS 250 260 270 280 290 300 PEDSFSLDSA NQQPHYGGKP PNVLTAAVPS GFPVEDRKSN TLNADAFLSA HTELFPTASS 310 320 330 340 350 360 GHLYPYDFQG DYVDQFTYDN HVPTPLMAIT ITRLVVGFLV PFFIMVICYS LIVFRMRKTN 370 380 390 400 410 420 FTKSRNKTFR VAVAVVTVFF ICWTPYHLVG VLLLITDPES SLGEAVMSWD HMSIALASAN 430 440 450 460 470 SCFNPFLYAL LGKDFRKKAR QSIKGILEAA FSEELTHSTN CTQDKASSKR NNMSTDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mouse anaphylatoxin C3a receptor: molecular cloning, genomic organization, and functional expression." Tornetta M.A., Foley J.J., Sarau H.M., Ames R.S. J. Immunol. 158:5277-5282(1997) [PubMed: 9164946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: 129/SvJ and C57BL/6. Tissue: Brain.
[2]	"Cloning and functional characterization of the mouse C3a anaphylatoxin receptor gene." Hsu M.H., Ember J.A., Wang M., Prossnitz E.R., Hugli T.E., Ye R.D. Immunogenetics 47:64-72(1997) [PubMed: 9382922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[3]	"Cloning, expression, sequence determination, and chromosome localization of the mouse complement C3a anaphylatoxin receptor gene." Hollmann T.J., Haviland D.L., Kildsgaard J., Watts K., Wetsel R.A. Mol. Immunol. 35:137-148(1998) [PubMed: 9694514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U77461 Genomic DNA. Translation: AAC53204.1. U77460 mRNA. Translation: AAC53203.1. U97537 Genomic DNA. Translation: AAB71814.1. AF053757 Genomic DNA. Translation: AAC40193.1. BC003728 mRNA. Translation: AAH03728.1.
IPI	IPI00113802.
RefSeq	NP_033909.1.
UniGene	Mm.2408
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSMUSG00000040552. Mus musculus. [Contig view]
GeneID	12267.
KEGG	mmu:12267.
Organism-specific databases
MGI	MGI:1097680. C3ar1.
Phylogenomic databases
HOGENOM	O09047.
HOVERGEN	O09047.
OMA	O09047. WVAGLKM.
Gene expression databases
ArrayExpress	O09047.
Bgee	O09047.
CleanEx	MM_C3AR1.
GermOnline	ENSMUSG00000040552. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR002234. Anphylx_rcpt. IPR001644. C3A_anaphtx_rcpt. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01104. ANPHYLATOXNR. PR01060. C3ANPHYLTXNR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	280718.
SOURCE	Search...

Entry information

Entry name

C3AR_MOUSE

Accession

Primary (citable) accession number: O09047
Secondary accession number(s): O35951

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)