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O09046

- OXLA_MOUSE

UniProt

O09046 - OXLA_MOUSE

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Protein
L-amino-acid oxidase
Gene
Il4i1, Fig1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation.1 Publication

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

FAD.

Kineticsi

  1. KM=6.5 mM for phenylalanine (at 37 degrees Celsius)

Vmax=0.0099 nmol/min/mg enzyme toward phenylalanine (at 37 degrees Celsius)

pH dependencei

Optimum pH is 4.0.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881FAD By similarity
Binding sitei96 – 961FAD By similarity
Binding sitei115 – 1151Substrate By similarity
Binding sitei286 – 2861FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei395 – 3951Substrate By similarity
Binding sitei479 – 4791FAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi114 – 1152FAD By similarity
Nucleotide bindingi488 – 4914FAD By similarity

GO - Molecular functioni

  1. L-amino-acid oxidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    ReactomeiREACT_189094. Abnormal metabolism in phenylketonuria.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-amino-acid oxidase (EC:1.4.3.2)
    Short name:
    LAAO
    Short name:
    LAO
    Alternative name(s):
    Interleukin-4-induced protein 1
    Short name:
    IL4-induced protein 1
    Protein Fig-1
    Short name:
    mFIG1
    Gene namesi
    Name:Il4i1
    Synonyms:Fig1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:109552. Il4i1.

    Subcellular locationi

    Isoform 1 : Lysosome 1 Publication
    Isoform 2 : Lysosome 1 Publication

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121 Reviewed prediction
    Add
    BLAST
    Chaini22 – 630609L-amino-acid oxidase
    PRO_0000001711Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi35 ↔ 198 By similarity
    Glycosylationi53 – 531N-linked (GlcNAc...) Reviewed prediction
    Glycosylationi133 – 1331N-linked (GlcNAc...) Reviewed prediction
    Glycosylationi219 – 2191N-linked (GlcNAc...) Reviewed prediction

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO09046.
    PRIDEiO09046.

    PTM databases

    PhosphoSiteiO09046.

    Expressioni

    Tissue specificityi

    Isoform 1 primarily found in immune tissues, mostly in B-lymphocytes. Isoform 2 restricted to the testis, predominantly in Sertoli cells at the periphery of the ducts, and the brain, including Purkinje cells, hippocampus and mitral cells in the olfactory bulb. No isoform 2 expression in fetal tissues.1 Publication

    Inductioni

    By interleukin-4.

    Gene expression databases

    ArrayExpressiO09046.
    BgeeiO09046.
    CleanExiMM_IL4I1.
    GenevestigatoriO09046.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-1727921.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2I8Wmodel-@30-502[»]
    ProteinModelPortaliO09046.
    SMRiO09046. Positions 28-506.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1231.
    GeneTreeiENSGT00730000110942.
    HOVERGENiHBG005729.
    KOiK03334.

    Family and domain databases

    InterProiIPR002937. Amino_oxidase.
    IPR001613. Flavin_amine_oxidase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    PRINTSiPR00757. AMINEOXDASEF.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative promoter usage. Align

    Isoform 1 (identifier: O09046-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGLALRLVL AATLLGLAGS LDWKAASSLN PIEKCMEDHD YEQLLKVVTL    50
    GLNRTSKPQK VVVVGAGVAG LVAAKMLSDA GHKVTILEAD NRIGGRIFTF 100
    RDEKTGWIGE LGAMRMPSSH RILHKLCRTL GLNLTQFTQY DENTWTEVHN 150
    VKLRNYVVEK MPEKLGYNLN NRERGHSPED IYQMALNKAF KDLKALGCKK 200
    AMNKFNKHTL LEYLLEEGNL SRPAVQLLGD VMSEEGFFYL SFAEALRAHA 250
    CLSDRLRYSR IVGGWDLLPR ALLSSLSGAL LLNAPVVSIT QGRNDVRVHI 300
    ATSLHSEKTL TADVVLLTAS GPALQRITFS PPLTRKRQEA LRALHYVAAS 350
    KVFLSFRRPF WHEEHIEGGH SNTDRPSRLI FYPARGEGSL LLASYTWSDA 400
    AAPFAGLSTD QTLRLVLQDV AALHGPVVFR LWDGRGVVKR WAEDPHSQGG 450
    FVVQPPLYGR EAEDYDWSAP FGRIYFAGEH TALPHGWVET AVKSGLRAAV 500
    RINNNYGYGE VDPQMMEHAY AEANYLDQYP EGERPEEQQA REEVSPDEQE 550
    PSHKHLLVET SPEGQQHAFV EAIPELQGHV FVETVPQEKG HAHQNIYPSE 600
    HVQVHGEVIP EWHGHGGSGT PQMHRVGDHS 630
    Length:630
    Mass (Da):70,191
    Last modified:July 1, 1997 - v1
    Checksum:iA674C5D60D89A071
    GO
    Isoform 2 (identifier: O09046-2) [UniParc]FASTAAdd to Basket

    Also known as: IL4I1_2

    The sequence of this isoform differs from the canonical sequence as follows:
         1-5: MAGLA → MGARRAPQRPPCT

    Note: Uses the promoter of the upstream NUP62 gene and shares the first 2 non-coding exons with NUP62.

    Show »
    Length:638
    Mass (Da):71,170
    Checksum:iD0425122748E7609
    GO

    Sequence cautioni

    The sequence BAB29253.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 55MAGLA → MGARRAPQRPPCT in isoform 2.
    VSP_017174

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121A → V in AAO17038. 1 Publication
    Sequence conflicti12 – 121A → V in AAO17039. 1 Publication
    Sequence conflicti56 – 561S → L in AAO65453. 1 Publication
    Sequence conflicti56 – 561S → L in AAS00457. 1 Publication
    Sequence conflicti184 – 1841M → V in BAE30174. 1 Publication
    Sequence conflicti184 – 1841M → V in BAE31293. 1 Publication
    Sequence conflicti385 – 3851R → Q in AAO23118. 1 Publication
    Sequence conflicti385 – 3851R → Q in BAE21502. 1 Publication
    Sequence conflicti385 – 3851R → Q in BAE29676. 1 Publication
    Sequence conflicti385 – 3851R → Q in BAE30047. 1 Publication
    Sequence conflicti385 – 3851R → Q in BAE30174. 1 Publication
    Sequence conflicti385 – 3851R → Q in BAE31293. 1 Publication
    Sequence conflicti454 – 4541Q → R in BAE21502. 1 Publication
    Sequence conflicti537 – 5371E → Q in BAE21502. 1 Publication
    Sequence conflicti551 – 5511P → L in AAO17038. 1 Publication
    Sequence conflicti551 – 5511P → L in AAO17039. 1 Publication
    Sequence conflicti568 – 5681A → M in AAO17038. 1 Publication
    Sequence conflicti568 – 5681A → M in AAO17039. 1 Publication
    Sequence conflicti598 – 63033PSEHV…VGDHS → LRSMYRCMGKSSLSGMVMGD LAPRKCTEWGTTPNRKEEVS TQLLSQPSSGQTDHLH in BAB29253. 1 Publication
    Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U70429 mRNA. Translation: AAB51353.1.
    U70430 Genomic RNA. Translation: AAB51354.1.
    AF538041 Genomic DNA. Translation: AAM15529.1.
    AY442170 Genomic DNA. Translation: AAM15530.2.
    AY157537 Genomic DNA. Translation: AAO17038.1.
    AY157538 mRNA. Translation: AAO17039.1.
    AY161348 Genomic DNA. Translation: AAO23118.1.
    AY178834 Genomic DNA. Translation: AAO65453.1.
    AY442343 Genomic DNA. Translation: AAS00457.1.
    AK014297 mRNA. Translation: BAB29253.1. Different initiation.
    AK133082 mRNA. Translation: BAE21502.1.
    AK150582 mRNA. Translation: BAE29676.1.
    AK151030 mRNA. Translation: BAE30047.1.
    AK151171 mRNA. Translation: BAE30174.1.
    AK152538 mRNA. Translation: BAE31293.1.
    AK172393 mRNA. Translation: BAE42981.1.
    BC115960 mRNA. Translation: AAI15961.1.
    U89428 Transcribed RNA. Translation: AAC36534.1.
    U89429 mRNA. Translation: AAC36535.1.
    CCDSiCCDS21217.1. [O09046-1]
    RefSeqiNP_001164495.1. NM_001171024.1.
    NP_034345.2. NM_010215.3.
    UniGeneiMm.2565.

    Genome annotation databases

    EnsembliENSMUST00000033015; ENSMUSP00000033015; ENSMUSG00000074141.
    GeneIDi100328588.
    14204.
    KEGGimmu:100328588.
    mmu:14204.

    Keywords - Coding sequence diversityi

    Alternative promoter usage

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U70429 mRNA. Translation: AAB51353.1 .
    U70430 Genomic RNA. Translation: AAB51354.1 .
    AF538041 Genomic DNA. Translation: AAM15529.1 .
    AY442170 Genomic DNA. Translation: AAM15530.2 .
    AY157537 Genomic DNA. Translation: AAO17038.1 .
    AY157538 mRNA. Translation: AAO17039.1 .
    AY161348 Genomic DNA. Translation: AAO23118.1 .
    AY178834 Genomic DNA. Translation: AAO65453.1 .
    AY442343 Genomic DNA. Translation: AAS00457.1 .
    AK014297 mRNA. Translation: BAB29253.1 . Different initiation.
    AK133082 mRNA. Translation: BAE21502.1 .
    AK150582 mRNA. Translation: BAE29676.1 .
    AK151030 mRNA. Translation: BAE30047.1 .
    AK151171 mRNA. Translation: BAE30174.1 .
    AK152538 mRNA. Translation: BAE31293.1 .
    AK172393 mRNA. Translation: BAE42981.1 .
    BC115960 mRNA. Translation: AAI15961.1 .
    U89428 Transcribed RNA. Translation: AAC36534.1 .
    U89429 mRNA. Translation: AAC36535.1 .
    CCDSi CCDS21217.1. [O09046-1 ]
    RefSeqi NP_001164495.1. NM_001171024.1.
    NP_034345.2. NM_010215.3.
    UniGenei Mm.2565.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2I8W model - @ 30-502 [» ]
    ProteinModelPortali O09046.
    SMRi O09046. Positions 28-506.
    ModBasei Search...

    Protein-protein interaction databases

    MINTi MINT-1727921.

    PTM databases

    PhosphoSitei O09046.

    Proteomic databases

    PaxDbi O09046.
    PRIDEi O09046.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033015 ; ENSMUSP00000033015 ; ENSMUSG00000074141 .
    GeneIDi 100328588.
    14204.
    KEGGi mmu:100328588.
    mmu:14204.

    Organism-specific databases

    CTDi 100328588.
    259307.
    MGIi MGI:109552. Il4i1.

    Phylogenomic databases

    eggNOGi COG1231.
    GeneTreei ENSGT00730000110942.
    HOVERGENi HBG005729.
    KOi K03334.

    Enzyme and pathway databases

    Reactomei REACT_189094. Abnormal metabolism in phenylketonuria.

    Miscellaneous databases

    NextBioi 285446.
    PROi O09046.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O09046.
    Bgeei O09046.
    CleanExi MM_IL4I1.
    Genevestigatori O09046.

    Family and domain databases

    InterProi IPR002937. Amino_oxidase.
    IPR001613. Flavin_amine_oxidase.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    PRINTSi PR00757. AMINEOXDASEF.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Fig1, an interleukin 4-induced mouse B cell gene isolated by cDNA representational difference analysis."
      Chu C.C., Paul W.E.
      Proc. Natl. Acad. Sci. U.S.A. 94:2507-2512(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
      Strain: BALB/c and CBA/J.
    2. "Interleukin-four induced gene-1 polymorphisms correlate with Sle3 autoimmune susceptibility."
      Chu C.C., Kim J.A., Gupta N., Yuen G.J., Thomas R.R., George J., Hsueh K.
      Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
      Strain: CBA/J, MRL and NZW.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J and NOD.
      Tissue: Bone marrow, Embryonic head, Spleen and Testis.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "Expressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis."
      Chu C.C., Paul W.E.
      Mol. Immunol. 35:487-502(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-289.
      Strain: BALB/c.
      Tissue: Spleen.
    6. "IL-4-induced gene-1 is a leukocyte L-amino acid oxidase with an unusual acidic pH preference and lysosomal localization."
      Mason J.M., Naidu M.D., Barcia M., Porti D., Chavan S.S., Chu C.C.
      J. Immunol. 173:4561-4567(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, FUNCTION, GLYCOSYLATION, SUBCELLULAR LOCATION.
    7. "Alternative pre-mRNA processing regulates cell-type specific expression of the IL4l1 and NUP62 genes."
      Wiemann S., Kolb-Kokocinski A., Poustka A.
      BMC Biol. 3:16-16(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE PROMOTER USAGE, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiOXLA_MOUSE
    AccessioniPrimary (citable) accession number: O09046
    Secondary accession number(s): Q1LZI6
    , Q3T9N9, Q3U7S6, Q3V0K2, Q6Y632, Q6YBV6, Q6YDI8, Q8R2G8, Q9CXK7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: July 1, 1997
    Last modified: September 3, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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