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O09046

- OXLA_MOUSE

UniProt

O09046 - OXLA_MOUSE

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Protein

L-amino-acid oxidase

Gene
Il4i1, Fig1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation.1 Publication

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

FAD.

Kineticsi

  1. KM=6.5 mM for phenylalanine (at 37 degrees Celsius)

Vmax=0.0099 nmol/min/mg enzyme toward phenylalanine (at 37 degrees Celsius)

pH dependencei

Optimum pH is 4.0.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881FAD By similarity
Binding sitei96 – 961FAD By similarity
Binding sitei115 – 1151Substrate By similarity
Binding sitei286 – 2861FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei395 – 3951Substrate By similarity
Binding sitei479 – 4791FAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi114 – 1152FAD By similarity
Nucleotide bindingi488 – 4914FAD By similarity

GO - Molecular functioni

  1. L-amino-acid oxidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiREACT_189094. Abnormal metabolism in phenylketonuria.

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino-acid oxidase (EC:1.4.3.2)
Short name:
LAAO
Short name:
LAO
Alternative name(s):
Interleukin-4-induced protein 1
Short name:
IL4-induced protein 1
Protein Fig-1
Short name:
mFIG1
Gene namesi
Name:Il4i1
Synonyms:Fig1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:109552. Il4i1.

Subcellular locationi

Isoform 1 : Lysosome 1 Publication
Isoform 2 : Lysosome 1 Publication

GO - Cellular componenti

  1. lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed predictionAdd
BLAST
Chaini22 – 630609L-amino-acid oxidasePRO_0000001711Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi35 ↔ 198 By similarity
Glycosylationi53 – 531N-linked (GlcNAc...) Reviewed prediction
Glycosylationi133 – 1331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi219 – 2191N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO09046.
PRIDEiO09046.

PTM databases

PhosphoSiteiO09046.

Expressioni

Tissue specificityi

Isoform 1 primarily found in immune tissues, mostly in B-lymphocytes. Isoform 2 restricted to the testis, predominantly in Sertoli cells at the periphery of the ducts, and the brain, including Purkinje cells, hippocampus and mitral cells in the olfactory bulb. No isoform 2 expression in fetal tissues.1 Publication

Inductioni

By interleukin-4.

Gene expression databases

ArrayExpressiO09046.
BgeeiO09046.
CleanExiMM_IL4I1.
GenevestigatoriO09046.

Interactioni

Protein-protein interaction databases

MINTiMINT-1727921.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I8Wmodel-@30-502[»]
ProteinModelPortaliO09046.
SMRiO09046. Positions 28-506.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1231.
GeneTreeiENSGT00730000110942.
HOVERGENiHBG005729.
KOiK03334.

Family and domain databases

InterProiIPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
PRINTSiPR00757. AMINEOXDASEF.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative promoter usage. Align

Isoform 1 (identifier: O09046-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAGLALRLVL AATLLGLAGS LDWKAASSLN PIEKCMEDHD YEQLLKVVTL    50
GLNRTSKPQK VVVVGAGVAG LVAAKMLSDA GHKVTILEAD NRIGGRIFTF 100
RDEKTGWIGE LGAMRMPSSH RILHKLCRTL GLNLTQFTQY DENTWTEVHN 150
VKLRNYVVEK MPEKLGYNLN NRERGHSPED IYQMALNKAF KDLKALGCKK 200
AMNKFNKHTL LEYLLEEGNL SRPAVQLLGD VMSEEGFFYL SFAEALRAHA 250
CLSDRLRYSR IVGGWDLLPR ALLSSLSGAL LLNAPVVSIT QGRNDVRVHI 300
ATSLHSEKTL TADVVLLTAS GPALQRITFS PPLTRKRQEA LRALHYVAAS 350
KVFLSFRRPF WHEEHIEGGH SNTDRPSRLI FYPARGEGSL LLASYTWSDA 400
AAPFAGLSTD QTLRLVLQDV AALHGPVVFR LWDGRGVVKR WAEDPHSQGG 450
FVVQPPLYGR EAEDYDWSAP FGRIYFAGEH TALPHGWVET AVKSGLRAAV 500
RINNNYGYGE VDPQMMEHAY AEANYLDQYP EGERPEEQQA REEVSPDEQE 550
PSHKHLLVET SPEGQQHAFV EAIPELQGHV FVETVPQEKG HAHQNIYPSE 600
HVQVHGEVIP EWHGHGGSGT PQMHRVGDHS 630
Length:630
Mass (Da):70,191
Last modified:July 1, 1997 - v1
Checksum:iA674C5D60D89A071
GO
Isoform 2 (identifier: O09046-2) [UniParc]FASTAAdd to Basket

Also known as: IL4I1_2

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MAGLA → MGARRAPQRPPCT

Note: Uses the promoter of the upstream NUP62 gene and shares the first 2 non-coding exons with NUP62.

Show »
Length:638
Mass (Da):71,170
Checksum:iD0425122748E7609
GO

Sequence cautioni

The sequence BAB29253.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 55MAGLA → MGARRAPQRPPCT in isoform 2. VSP_017174

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121A → V in AAO17038. 1 Publication
Sequence conflicti12 – 121A → V in AAO17039. 1 Publication
Sequence conflicti56 – 561S → L in AAO65453. 1 Publication
Sequence conflicti56 – 561S → L in AAS00457. 1 Publication
Sequence conflicti184 – 1841M → V in BAE30174. 1 Publication
Sequence conflicti184 – 1841M → V in BAE31293. 1 Publication
Sequence conflicti385 – 3851R → Q in AAO23118. 1 Publication
Sequence conflicti385 – 3851R → Q in BAE21502. 1 Publication
Sequence conflicti385 – 3851R → Q in BAE29676. 1 Publication
Sequence conflicti385 – 3851R → Q in BAE30047. 1 Publication
Sequence conflicti385 – 3851R → Q in BAE30174. 1 Publication
Sequence conflicti385 – 3851R → Q in BAE31293. 1 Publication
Sequence conflicti454 – 4541Q → R in BAE21502. 1 Publication
Sequence conflicti537 – 5371E → Q in BAE21502. 1 Publication
Sequence conflicti551 – 5511P → L in AAO17038. 1 Publication
Sequence conflicti551 – 5511P → L in AAO17039. 1 Publication
Sequence conflicti568 – 5681A → M in AAO17038. 1 Publication
Sequence conflicti568 – 5681A → M in AAO17039. 1 Publication
Sequence conflicti598 – 63033PSEHV…VGDHS → LRSMYRCMGKSSLSGMVMGD LAPRKCTEWGTTPNRKEEVS TQLLSQPSSGQTDHLH in BAB29253. 1 PublicationAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U70429 mRNA. Translation: AAB51353.1.
U70430 Genomic RNA. Translation: AAB51354.1.
AF538041 Genomic DNA. Translation: AAM15529.1.
AY442170 Genomic DNA. Translation: AAM15530.2.
AY157537 Genomic DNA. Translation: AAO17038.1.
AY157538 mRNA. Translation: AAO17039.1.
AY161348 Genomic DNA. Translation: AAO23118.1.
AY178834 Genomic DNA. Translation: AAO65453.1.
AY442343 Genomic DNA. Translation: AAS00457.1.
AK014297 mRNA. Translation: BAB29253.1. Different initiation.
AK133082 mRNA. Translation: BAE21502.1.
AK150582 mRNA. Translation: BAE29676.1.
AK151030 mRNA. Translation: BAE30047.1.
AK151171 mRNA. Translation: BAE30174.1.
AK152538 mRNA. Translation: BAE31293.1.
AK172393 mRNA. Translation: BAE42981.1.
BC115960 mRNA. Translation: AAI15961.1.
U89428 Transcribed RNA. Translation: AAC36534.1.
U89429 mRNA. Translation: AAC36535.1.
CCDSiCCDS21217.1. [O09046-1]
RefSeqiNP_001164495.1. NM_001171024.1.
NP_034345.2. NM_010215.3.
UniGeneiMm.2565.

Genome annotation databases

EnsembliENSMUST00000033015; ENSMUSP00000033015; ENSMUSG00000074141.
GeneIDi100328588.
14204.
KEGGimmu:100328588.
mmu:14204.

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U70429 mRNA. Translation: AAB51353.1 .
U70430 Genomic RNA. Translation: AAB51354.1 .
AF538041 Genomic DNA. Translation: AAM15529.1 .
AY442170 Genomic DNA. Translation: AAM15530.2 .
AY157537 Genomic DNA. Translation: AAO17038.1 .
AY157538 mRNA. Translation: AAO17039.1 .
AY161348 Genomic DNA. Translation: AAO23118.1 .
AY178834 Genomic DNA. Translation: AAO65453.1 .
AY442343 Genomic DNA. Translation: AAS00457.1 .
AK014297 mRNA. Translation: BAB29253.1 . Different initiation.
AK133082 mRNA. Translation: BAE21502.1 .
AK150582 mRNA. Translation: BAE29676.1 .
AK151030 mRNA. Translation: BAE30047.1 .
AK151171 mRNA. Translation: BAE30174.1 .
AK152538 mRNA. Translation: BAE31293.1 .
AK172393 mRNA. Translation: BAE42981.1 .
BC115960 mRNA. Translation: AAI15961.1 .
U89428 Transcribed RNA. Translation: AAC36534.1 .
U89429 mRNA. Translation: AAC36535.1 .
CCDSi CCDS21217.1. [O09046-1 ]
RefSeqi NP_001164495.1. NM_001171024.1.
NP_034345.2. NM_010215.3.
UniGenei Mm.2565.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2I8W model - @ 30-502 [» ]
ProteinModelPortali O09046.
SMRi O09046. Positions 28-506.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1727921.

PTM databases

PhosphoSitei O09046.

Proteomic databases

PaxDbi O09046.
PRIDEi O09046.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033015 ; ENSMUSP00000033015 ; ENSMUSG00000074141 .
GeneIDi 100328588.
14204.
KEGGi mmu:100328588.
mmu:14204.

Organism-specific databases

CTDi 100328588.
259307.
MGIi MGI:109552. Il4i1.

Phylogenomic databases

eggNOGi COG1231.
GeneTreei ENSGT00730000110942.
HOVERGENi HBG005729.
KOi K03334.

Enzyme and pathway databases

Reactomei REACT_189094. Abnormal metabolism in phenylketonuria.

Miscellaneous databases

NextBioi 285446.
PROi O09046.
SOURCEi Search...

Gene expression databases

ArrayExpressi O09046.
Bgeei O09046.
CleanExi MM_IL4I1.
Genevestigatori O09046.

Family and domain databases

InterProi IPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
PRINTSi PR00757. AMINEOXDASEF.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Fig1, an interleukin 4-induced mouse B cell gene isolated by cDNA representational difference analysis."
    Chu C.C., Paul W.E.
    Proc. Natl. Acad. Sci. U.S.A. 94:2507-2512(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
    Strain: BALB/c and CBA/J.
  2. "Interleukin-four induced gene-1 polymorphisms correlate with Sle3 autoimmune susceptibility."
    Chu C.C., Kim J.A., Gupta N., Yuen G.J., Thomas R.R., George J., Hsueh K.
    Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
    Strain: CBA/J, MRL and NZW.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Embryonic head, Spleen and Testis.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Expressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis."
    Chu C.C., Paul W.E.
    Mol. Immunol. 35:487-502(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-289.
    Strain: BALB/c.
    Tissue: Spleen.
  6. "IL-4-induced gene-1 is a leukocyte L-amino acid oxidase with an unusual acidic pH preference and lysosomal localization."
    Mason J.M., Naidu M.D., Barcia M., Porti D., Chavan S.S., Chu C.C.
    J. Immunol. 173:4561-4567(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, FUNCTION, GLYCOSYLATION, SUBCELLULAR LOCATION.
  7. "Alternative pre-mRNA processing regulates cell-type specific expression of the IL4l1 and NUP62 genes."
    Wiemann S., Kolb-Kokocinski A., Poustka A.
    BMC Biol. 3:16-16(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE PROMOTER USAGE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiOXLA_MOUSE
AccessioniPrimary (citable) accession number: O09046
Secondary accession number(s): Q1LZI6
, Q3T9N9, Q3U7S6, Q3V0K2, Q6Y632, Q6YBV6, Q6YDI8, Q8R2G8, Q9CXK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: September 3, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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