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Protein

Synaptosomal-associated protein 23

Gene

Snap23

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1236974. ER-Phagosome pathway.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-421837. Clathrin derived vesicle budding.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptosomal-associated protein 23
Short name:
SNAP-23
Alternative name(s):
Syndet
Vesicle-membrane fusion protein SNAP-23
Gene namesi
Name:Snap23
Synonyms:Sndt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:109356. Snap23.

Subcellular locationi

GO - Cellular componenti

  • azurophil granule Source: MGI
  • cell-cell junction Source: MGI
  • cytoplasm Source: MGI
  • cytoplasmic vesicle Source: MGI
  • extracellular exosome Source: MGI
  • focal adhesion Source: MGI
  • mast cell granule Source: GOC
  • neuron projection Source: UniProtKB-SubCell
  • nucleoplasm Source: MGI
  • plasma membrane Source: MGI
  • presynapse Source: GOC
  • SNARE complex Source: MGI
  • specific granule Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi119P → A: Inhibits interaction with ZDHHC13 and ZDHHC17. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135991 – 210Synaptosomal-associated protein 23Add BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei5PhosphoserineBy similarity1
Modified residuei20PhosphoserineCombined sources1
Modified residuei23PhosphoserineCombined sources1
Modified residuei34PhosphoserineCombined sources1
Lipidationi79S-palmitoyl cysteineBy similarity1
Lipidationi80S-palmitoyl cysteineBy similarity1
Lipidationi83S-palmitoyl cysteineBy similarity1
Lipidationi85S-palmitoyl cysteineBy similarity1
Lipidationi87S-palmitoyl cysteineBy similarity1
Modified residuei110PhosphoserineCombined sources1
Modified residuei160PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiO09044.
MaxQBiO09044.
PaxDbiO09044.
PRIDEiO09044.

PTM databases

iPTMnetiO09044.
PhosphoSitePlusiO09044.
SwissPalmiO09044.

Expressioni

Tissue specificityi

Expressed in non-neuronal tissues.

Gene expression databases

BgeeiENSMUSG00000027287.
CleanExiMM_SNAP23.
ExpressionAtlasiO09044. baseline and differential.
GenevisibleiO09044. MM.

Interactioni

Subunit structurei

Homotetramer (via coiled-coil domain), also forms heterotetramers with STX4 and VAMP3 (By similarity). Found in a complex with VAMP8 and STX1A (By similarity). Found in a complex with VAMP8 and STX4 in pancreas (PubMed:15363411). Interacts simultaneously with SNAPIN and SYN4 (By similarity). Interacts with STX1A (PubMed:9507000). Interacts with STX12 (PubMed:9507000). Interacts tightly to multiple syntaxins and synaptobrevins/VAMPs (By similarity). Interacts with ZDHHC13 (via ANK repeats) (PubMed:26198635). Interacts with ZDHHC17 (via ANK repeats) (PubMed:26198635).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Stx4P704522EBI-1812522,EBI-645716
Stx4Q088504EBI-1812522,EBI-918243From a different organism.
Vamp2P630442EBI-1812522,EBI-521920

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203367. 10 interactors.
DIPiDIP-41401N.
IntActiO09044. 9 interactors.
MINTiMINT-269238.
STRINGi10090.ENSMUSP00000112138.

Structurei

3D structure databases

ProteinModelPortaliO09044.
SMRiO09044.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 76t-SNARE coiled-coil homology 1PROSITE-ProRule annotationAdd BLAST63
Domaini145 – 207t-SNARE coiled-coil homology 2PROSITE-ProRule annotationAdd BLAST63

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili23 – 76By similarityAdd BLAST54

Sequence similaritiesi

Belongs to the SNAP-25 family.Curated
Contains 2 t-SNARE coiled-coil homology domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG3065. Eukaryota.
ENOG410Y3Y0. LUCA.
GeneTreeiENSGT00390000012186.
HOGENOMiHOG000231599.
HOVERGENiHBG056971.
InParanoidiO09044.
KOiK08508.
PhylomeDBiO09044.

Family and domain databases

InterProiIPR000928. SNAP-25.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF00835. SNAP-25. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 2 hits.
[Graphical view]
PROSITEiPS50192. T_SNARE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O09044-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNLSPEEVQ LRAHQVTDES LESTRRILGL AIESQDAGIK TITMLDEQGE
60 70 80 90 100
QLNRIEEGMD QINKDMREAE KTLTELNKCC GLCICPCNRT KNFESGKNYK
110 120 130 140 150
ATWGDGGDNS PSNVVSKQPS RITNGQPQQT TGAASGGYIK RITNDAREDE
160 170 180 190 200
MEENLTQVGS ILGNLKNMAL DMGNEIDAQN QQIQKITEKA DTNKNRIDIA
210
NTRAKKLIDS
Length:210
Mass (Da):23,261
Last modified:July 1, 1997 - v1
Checksum:i6919E127E16BA2C9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6P → S in AAB62932 (Ref. 3) Curated1
Sequence conflicti204A → P in AAB62932 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000822 mRNA. Translation: BAA20345.1.
U73143 mRNA. Translation: AAB53597.1.
AF007169 mRNA. Translation: AAB62932.1.
AF213257
, AF213251, AF213252, AF213253, AF213254, AF213255, AF213256 Genomic DNA. Translation: AAF23503.1.
AK019162 mRNA. Translation: BAB31577.1.
BC070456 mRNA. Translation: AAH70456.1.
CCDSiCCDS16622.1.
PIRiJC5512.
RefSeqiNP_001171263.1. NM_001177792.1.
NP_001171264.1. NM_001177793.1.
NP_033248.1. NM_009222.3.
XP_006499120.1. XM_006499057.3.
UniGeneiMm.245715.

Genome annotation databases

EnsembliENSMUST00000028743; ENSMUSP00000028743; ENSMUSG00000027287.
ENSMUST00000110711; ENSMUSP00000106339; ENSMUSG00000027287.
GeneIDi20619.
KEGGimmu:20619.
UCSCiuc008lwg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000822 mRNA. Translation: BAA20345.1.
U73143 mRNA. Translation: AAB53597.1.
AF007169 mRNA. Translation: AAB62932.1.
AF213257
, AF213251, AF213252, AF213253, AF213254, AF213255, AF213256 Genomic DNA. Translation: AAF23503.1.
AK019162 mRNA. Translation: BAB31577.1.
BC070456 mRNA. Translation: AAH70456.1.
CCDSiCCDS16622.1.
PIRiJC5512.
RefSeqiNP_001171263.1. NM_001177792.1.
NP_001171264.1. NM_001177793.1.
NP_033248.1. NM_009222.3.
XP_006499120.1. XM_006499057.3.
UniGeneiMm.245715.

3D structure databases

ProteinModelPortaliO09044.
SMRiO09044.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203367. 10 interactors.
DIPiDIP-41401N.
IntActiO09044. 9 interactors.
MINTiMINT-269238.
STRINGi10090.ENSMUSP00000112138.

PTM databases

iPTMnetiO09044.
PhosphoSitePlusiO09044.
SwissPalmiO09044.

Proteomic databases

EPDiO09044.
MaxQBiO09044.
PaxDbiO09044.
PRIDEiO09044.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028743; ENSMUSP00000028743; ENSMUSG00000027287.
ENSMUST00000110711; ENSMUSP00000106339; ENSMUSG00000027287.
GeneIDi20619.
KEGGimmu:20619.
UCSCiuc008lwg.1. mouse.

Organism-specific databases

CTDi8773.
MGIiMGI:109356. Snap23.

Phylogenomic databases

eggNOGiKOG3065. Eukaryota.
ENOG410Y3Y0. LUCA.
GeneTreeiENSGT00390000012186.
HOGENOMiHOG000231599.
HOVERGENiHBG056971.
InParanoidiO09044.
KOiK08508.
PhylomeDBiO09044.

Enzyme and pathway databases

ReactomeiR-MMU-1236974. ER-Phagosome pathway.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-421837. Clathrin derived vesicle budding.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiO09044.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027287.
CleanExiMM_SNAP23.
ExpressionAtlasiO09044. baseline and differential.
GenevisibleiO09044. MM.

Family and domain databases

InterProiIPR000928. SNAP-25.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF00835. SNAP-25. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 2 hits.
[Graphical view]
PROSITEiPS50192. T_SNARE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSNP23_MOUSE
AccessioniPrimary (citable) accession number: O09044
Secondary accession number(s): O35620
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.