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O09044 (SNP23_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synaptosomal-associated protein 23

Short name=SNAP-23
Alternative name(s):
Syndet
Vesicle-membrane fusion protein SNAP-23
Gene names
Name:Snap23
Synonyms:Sndt
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion By similarity.

Subunit structure

Homotetramer (via coiled-coil domain), also forms heterotetramers with STX4 and VAMP3. Binds simultaneously to SNAPIN and SYN4. Found in a complex with VAMP8 and STX1A By similarity. Binds tightly to multiple syntaxins and synaptobrevins/VAMPs. Found in a complex with VAMP8 and STX4 in pancreas. Interacts with STX1A and STX12. Ref.7 Ref.8

Subcellular location

Cell membrane; Peripheral membrane protein. Cell membrane; Lipid-anchor By similarity. Cell junctionsynapsesynaptosome. Note: Mainly localized to the plasma membrane.

Tissue specificity

Expressed in non-neuronal tissues.

Sequence similarities

Belongs to the SNAP-25 family.

Contains 2 t-SNARE coiled-coil homology domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210Synaptosomal-associated protein 23
PRO_0000213599

Regions

Domain14 – 7663t-SNARE coiled-coil homology 1
Domain145 – 20763t-SNARE coiled-coil homology 2
Coiled coil23 – 7654 By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue201Phosphoserine Ref.11
Modified residue231Phosphoserine Ref.11
Modified residue341Phosphoserine Ref.11
Modified residue1101Phosphoserine Ref.10 Ref.11
Lipidation791S-palmitoyl cysteine By similarity
Lipidation801S-palmitoyl cysteine By similarity
Lipidation831S-palmitoyl cysteine By similarity
Lipidation851S-palmitoyl cysteine By similarity
Lipidation871S-palmitoyl cysteine By similarity

Experimental info

Sequence conflict61P → S in AAB62932. Ref.3
Sequence conflict2041A → P in AAB62932. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O09044 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 6919E127E16BA2C9

FASTA21023,261
        10         20         30         40         50         60 
MDNLSPEEVQ LRAHQVTDES LESTRRILGL AIESQDAGIK TITMLDEQGE QLNRIEEGMD 

        70         80         90        100        110        120 
QINKDMREAE KTLTELNKCC GLCICPCNRT KNFESGKNYK ATWGDGGDNS PSNVVSKQPS 

       130        140        150        160        170        180 
RITNGQPQQT TGAASGGYIK RITNDAREDE MEENLTQVGS ILGNLKNMAL DMGNEIDAQN 

       190        200        210 
QQIQKITEKA DTNKNRIDIA NTRAKKLIDS 

« Hide

References

« Hide 'large scale' references
[1]"Inhibition of the binding of SNAP-23 to syntaxin 4 by Munc18c."
Araki S., Tamori Y., Kawanishi M., Shinoda H., Masugi J., Mori H., Niki T., Okazawa H., Kubota T., Kasuga M.
Biochem. Biophys. Res. Commun. 234:257-262(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Adipose tissue.
[2]"Syndet is a novel SNAP-25 related protein expressed in many tissues."
Wang G., Witkin J.W., Hao G., Bankaitis V.A., Scherer P.E., Baldini G.
J. Cell Sci. 110:505-513(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"SNARE expression in mouse plasma cells."
Olken S.K., Doerre S., Corley R.B.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Structure and chromosomal localization of the mouse SNAP-23 gene."
Vaidyanathan V.V., Roche P.A.
Gene 247:181-189(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryo.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Eye.
[7]"Syntaxin 12, a member of the syntaxin family localized to the endosome."
Tang B.L., Tan A.E., Lim L.K., Lee S.S., Low D.Y., Hong W.
J. Biol. Chem. 273:6944-6950(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH STX1A AND STX12.
[8]"A role of VAMP8/endobrevin in regulated exocytosis of pancreatic acinar cells."
Wang C.-C., Ng C.P., Lu L., Atlashkin V., Zhang W., Seet L.-F., Hong W.
Dev. Cell 7:359-371(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH VAMP8 AND STX4.
[9]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[10]"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[11]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20; SER-23; SER-34 AND SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB000822 mRNA. Translation: BAA20345.1.
U73143 mRNA. Translation: AAB53597.1.
AF007169 mRNA. Translation: AAB62932.1.
AF213257 expand/collapse EMBL AC list , AF213251, AF213252, AF213253, AF213254, AF213255, AF213256 Genomic DNA. Translation: AAF23503.1.
AK019162 mRNA. Translation: BAB31577.1.
BC070456 mRNA. Translation: AAH70456.1.
CCDSCCDS16622.1.
PIRJC5512.
RefSeqNP_001171263.1. NM_001177792.1.
NP_001171264.1. NM_001177793.1.
NP_033248.1. NM_009222.3.
XP_006499120.1. XM_006499057.1.
UniGeneMm.245715.

3D structure databases

ProteinModelPortalO09044.
SMRO09044. Positions 23-76, 146-204.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203367. 7 interactions.
IntActO09044. 6 interactions.
MINTMINT-269238.

PTM databases

PhosphoSiteO09044.

Proteomic databases

MaxQBO09044.
PaxDbO09044.
PRIDEO09044.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028743; ENSMUSP00000028743; ENSMUSG00000027287.
ENSMUST00000110711; ENSMUSP00000106339; ENSMUSG00000027287.
GeneID20619.
KEGGmmu:20619.
UCSCuc008lwg.1. mouse.

Organism-specific databases

CTD8773.
MGIMGI:109356. Snap23.

Phylogenomic databases

eggNOGNOG259235.
GeneTreeENSGT00390000012186.
HOGENOMHOG000231599.
HOVERGENHBG056971.
KOK08508.
OrthoDBEOG75F4F5.
PhylomeDBO09044.

Enzyme and pathway databases

ReactomeREACT_147847. Translocation of Glut4 to the Plasma Membrane.
REACT_209837. Membrane Trafficking.

Gene expression databases

ArrayExpressO09044.
BgeeO09044.
CleanExMM_SNAP23.
GenevestigatorO09044.

Family and domain databases

InterProIPR000928. SNAP-25.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamPF00835. SNAP-25. 1 hit.
PF05739. SNARE. 1 hit.
[Graphical view]
SMARTSM00397. t_SNARE. 2 hits.
[Graphical view]
PROSITEPS50192. T_SNARE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio299005.
PROO09044.
SOURCESearch...

Entry information

Entry nameSNP23_MOUSE
AccessionPrimary (citable) accession number: O09044
Secondary accession number(s): O35620
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot