O09044 (SNP23_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Synaptosomal-associated protein 23
Short name=SNAP-23

Alternative name(s):
Syndet
Vesicle-membrane fusion protein SNAP-23

Gene names

Name:	Snap23
Synonyms:	Sndt

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion By similarity.
Subunit structure	Homotetramer (via coiled-coil domain), also forms heterotetramers with STX4 and VAMP3. Binds simultaneously to SNAPIN and SYN4. Found in a complex with VAMP8 and STX1A By similarity. Binds tightly to multiple syntaxins and synaptobrevins/VAMPs. Found in a complex with VAMP8 and STX4 in pancreas. Interacts with STX1A and STX12. Ref.7 Ref.8
Subcellular location	Cell membrane; Peripheral membrane protein. Cell membrane; Lipid-anchor By similarity. Cell junction › synapse › synaptosome. Note: Mainly localized to the plasma membrane.
Tissue specificity	Expressed in non-neuronal tissues.
Sequence similarities	Belongs to the SNAP-25 family. Contains 2 t-SNARE coiled-coil homology domains.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell junction Cell membrane Membrane Synapse Synaptosome
Domain	Coiled coil Repeat
PTM	Acetylation Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	exocytosis Inferred from mutant phenotype PubMed 18505797. Source: MGI protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasmic vesicle Inferred from direct assay PubMed 18505797. Source: MGI neuron projection Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 18505797. Source: MGI synapse Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Synaptosomal-associated protein 23		PRO_0000213599
Regions
	Domain	14 – 76	63	t-SNARE coiled-coil homology 1
	Domain	145 – 207	63	t-SNARE coiled-coil homology 2
	Coiled coil	23 – 76	54	By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	20	1	Phosphoserine Ref.12
	Modified residue	34	1	Phosphoserine Ref.12
	Modified residue	110	1	Phosphoserine Ref.10 Ref.11 Ref.12
	Modified residue	112	1	Phosphoserine Ref.9
	Lipidation	79	1	S-palmitoyl cysteine By similarity
	Lipidation	80	1	S-palmitoyl cysteine By similarity
	Lipidation	83	1	S-palmitoyl cysteine By similarity
	Lipidation	85	1	S-palmitoyl cysteine By similarity
	Lipidation	87	1	S-palmitoyl cysteine By similarity
Experimental info
	Sequence conflict	6	1	P → S in AAB62932. Ref.3
	Sequence conflict	204	1	A → P in AAB62932. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O09044 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 6919E127E16BA2C9	FASTA	210	23,261
	10 20 30 40 50 60 MDNLSPEEVQ LRAHQVTDES LESTRRILGL AIESQDAGIK TITMLDEQGE QLNRIEEGMD 70 80 90 100 110 120 QINKDMREAE KTLTELNKCC GLCICPCNRT KNFESGKNYK ATWGDGGDNS PSNVVSKQPS 130 140 150 160 170 180 RITNGQPQQT TGAASGGYIK RITNDAREDE MEENLTQVGS ILGNLKNMAL DMGNEIDAQN 190 200 210 QQIQKITEKA DTNKNRIDIA NTRAKKLIDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Inhibition of the binding of SNAP-23 to syntaxin 4 by Munc18c." Araki S., Tamori Y., Kawanishi M., Shinoda H., Masugi J., Mori H., Niki T., Okazawa H., Kubota T., Kasuga M. Biochem. Biophys. Res. Commun. 234:257-262(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adipose tissue.
[2]	"Syndet is a novel SNAP-25 related protein expressed in many tissues." Wang G., Witkin J.W., Hao G., Bankaitis V.A., Scherer P.E., Baldini G. J. Cell Sci. 110:505-513(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"SNARE expression in mouse plasma cells." Olken S.K., Doerre S., Corley R.B. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Structure and chromosomal localization of the mouse SNAP-23 gene." Vaidyanathan V.V., Roche P.A. Gene 247:181-189(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Eye.
[7]	"Syntaxin 12, a member of the syntaxin family localized to the endosome." Tang B.L., Tan A.E., Lim L.K., Lee S.S., Low D.Y., Hong W. J. Biol. Chem. 273:6944-6950(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH STX1A AND STX12.
[8]	"A role of VAMP8/endobrevin in regulated exocytosis of pancreatic acinar cells." Wang C.-C., Ng C.P., Lu L., Atlashkin V., Zhang W., Seet L.-F., Hong W. Dev. Cell 7:359-371(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH VAMP8 AND STX4.
[9]	"Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line." Shu H., Chen S., Bi Q., Mumby M., Brekken D.L. Mol. Cell. Proteomics 3:279-286(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[10]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, MASS SPECTROMETRY. Tissue: Liver.
[11]	"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H. J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, MASS SPECTROMETRY. Tissue: Liver.
[12]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20; SER-34 AND SER-110, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB000822 mRNA. Translation: BAA20345.1. U73143 mRNA. Translation: AAB53597.1. AF007169 mRNA. Translation: AAB62932.1. AF213257 , AF213251, AF213252, AF213253, AF213254, AF213255, AF213256 Genomic DNA. Translation: AAF23503.1. AK019162 mRNA. Translation: BAB31577.1. BC070456 mRNA. Translation: AAH70456.1.
IPI	IPI00622270.
PIR	JC5512.
RefSeq	NP_001171263.1. NM_001177792.1. NP_001171264.1. NM_001177793.1. NP_033248.1. NM_009222.3.
UniGene	Mm.245715.
3D structure databases
ProteinModelPortal	O09044.
SMR	O09044. Positions 23-76, 146-204.
ModBase	Search...
Protein-protein interaction databases
IntAct	O09044. 5 interactions.
MINT	MINT-269238.
PTM databases
PhosphoSite	O09044.
Proteomic databases
PaxDb	O09044.
PRIDE	O09044.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000028743; ENSMUSP00000028743; ENSMUSG00000027287. ENSMUST00000110711; ENSMUSP00000106339; ENSMUSG00000027287.
GeneID	20619.
KEGG	mmu:20619.
UCSC	uc008lwg.1. mouse.
Organism-specific databases
CTD	8773.
MGI	MGI:109356. Snap23.
Phylogenomic databases
eggNOG	NOG259235.
GeneTree	ENSGT00390000012186.
HOGENOM	HOG000231599.
HOVERGEN	HBG056971.
KO	K08508.
OrthoDB	EOG4H9XMG.
Enzyme and pathway databases
Reactome	REACT_147847. Translocation of Glut4 to the Plasma Membrane. REACT_88307. Membrane Trafficking.
Gene expression databases
ArrayExpress	O09044.
Bgee	O09044.
CleanEx	MM_SNAP23.
Genevestigator	O09044.
GermOnline	ENSMUSG00000027287. Mus musculus.
Family and domain databases
InterPro	IPR000928. SNAP-25. IPR000727. T_SNARE_dom. [Graphical view]
Pfam	PF00835. SNAP-25. 1 hit. PF05739. SNARE. 1 hit. [Graphical view]
SMART	SM00397. t_SNARE. 2 hits. [Graphical view]
PROSITE	PS50192. T_SNARE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	299005.
SOURCE	Search...

Entry information

Entry name

SNP23_MOUSE

Accession

Primary (citable) accession number: O09044
Secondary accession number(s): O35620

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	July 1, 1997
Last modified:	May 29, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program