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Protein

Napsin-A

Gene

Napsa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in processing of pneumocyte surfactant precursors.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei91 – 911PROSITE-ProRule annotation
Active sitei278 – 2781PROSITE-ProRule annotation

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: UniProtKB-KW
  • endopeptidase activity Source: UniProtKB
  • peptidase activity Source: MGI

GO - Biological processi

  • membrane protein proteolysis Source: UniProtKB
  • surfactant homeostasis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.23.B1. 3474.
ReactomeiR-MMU-5683826. Surfactant metabolism.

Protein family/group databases

MEROPSiA01.046.

Names & Taxonomyi

Protein namesi
Recommended name:
Napsin-A (EC:3.4.23.-)
Alternative name(s):
KDAP-1
Kidney-derived aspartic protease-like protein
Short name:
KAP
Gene namesi
Name:Napsa
Synonyms:Kdap, Nap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:109365. Napsa.

Subcellular locationi

GO - Cellular componenti

  • alveolar lamellar body Source: UniProtKB
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • lysosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 419Napsin-APRO_0000025999
Signal peptidei1 – 1616Sequence analysisAdd
BLAST
Propeptidei17 – ?Activation peptideSequence analysisPRO_0000025998

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence analysis
Disulfide bondi104 ↔ 111By similarity
Glycosylationi128 – 1281N-linked (GlcNAc...)1 Publication
Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence analysis
Disulfide bondi269 ↔ 273By similarity
Disulfide bondi312 ↔ 349By similarity
Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiO09043.
PaxDbiO09043.
PRIDEiO09043.

PTM databases

PhosphoSiteiO09043.

Expressioni

Tissue specificityi

Expressed at the highest levels in the kidney, at a moderate level in the lung, and at low levels in the spleen and adipose tissue.1 Publication

Gene expression databases

BgeeiO09043.
CleanExiMM_NAPSA.
ExpressionAtlasiO09043. baseline and differential.
GenevisibleiO09043. MM.

Interactioni

Protein-protein interaction databases

IntActiO09043. 3 interactions.
MINTiMINT-4103045.
STRINGi10090.ENSMUSP00000002274.

Structurei

3D structure databases

ProteinModelPortaliO09043.
SMRiO09043. Positions 63-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini73 – 394322Peptidase A1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiO09043.
KOiK08565.
OMAiIILCSEI.
OrthoDBiEOG7HQN88.
PhylomeDBiO09043.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O09043-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPLLLLLLC LLLGNLEPEE AKLIRVPLQR IHLGHRILNP LNGWEQLAEL
60 70 80 90 100
SRTSTSGGNP SFVPLSKFMN TQYFGTIGLG TPPQNFTVVF DTGSSNLWVP
110 120 130 140 150
STRCHFFSLA CWFHHRFNPK ASSSFRPNGT KFAIQYGTGR LSGILSQDNL
160 170 180 190 200
TIGGIHDAFV TFGEALWEPS LIFALAHFDG ILGLGFPTLA VGGVQPPLDA
210 220 230 240 250
MVEQGLLEKP VFSFYLNRDS EGSDGGELVL GGSDPAHYVP PLTFIPVTIP
260 270 280 290 300
AYWQVHMESV KVGTGLSLCA QGCSAILDTG TSLITGPSEE IRALNKAIGG
310 320 330 340 350
YPFLNGQYFI QCSKTPTLPP VSFHLGGVWF NLTGQDYVIK ILQSDVGLCL
360 370 380 390 400
LGFQALDIPK PAGPLWILGD VFLGPYVAVF DRGDKNVGPR VGLARAQSRS
410
TDRAERRTTQ AQFFKRRPG
Length:419
Mass (Da):45,544
Last modified:July 1, 1997 - v1
Checksum:i715ED4532791143E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88899 mRNA. Translation: BAA19004.1.
AJ250718, AJ250719, AJ250720 Genomic DNA. Translation: CAB82907.1.
BC014813 mRNA. Translation: AAH14813.1.
CCDSiCCDS21212.1.
RefSeqiNP_032463.1. NM_008437.1.
UniGeneiMm.383181.

Genome annotation databases

EnsembliENSMUST00000002274; ENSMUSP00000002274; ENSMUSG00000002204.
GeneIDi16541.
KEGGimmu:16541.
UCSCiuc009gqe.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88899 mRNA. Translation: BAA19004.1.
AJ250718, AJ250719, AJ250720 Genomic DNA. Translation: CAB82907.1.
BC014813 mRNA. Translation: AAH14813.1.
CCDSiCCDS21212.1.
RefSeqiNP_032463.1. NM_008437.1.
UniGeneiMm.383181.

3D structure databases

ProteinModelPortaliO09043.
SMRiO09043. Positions 63-396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO09043. 3 interactions.
MINTiMINT-4103045.
STRINGi10090.ENSMUSP00000002274.

Protein family/group databases

MEROPSiA01.046.

PTM databases

PhosphoSiteiO09043.

Proteomic databases

EPDiO09043.
PaxDbiO09043.
PRIDEiO09043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002274; ENSMUSP00000002274; ENSMUSG00000002204.
GeneIDi16541.
KEGGimmu:16541.
UCSCiuc009gqe.1. mouse.

Organism-specific databases

CTDi9476.
MGIiMGI:109365. Napsa.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiO09043.
KOiK08565.
OMAiIILCSEI.
OrthoDBiEOG7HQN88.
PhylomeDBiO09043.
TreeFamiTF314990.

Enzyme and pathway databases

BRENDAi3.4.23.B1. 3474.
ReactomeiR-MMU-5683826. Surfactant metabolism.

Miscellaneous databases

ChiTaRSiNapsa. mouse.
PROiO09043.
SOURCEiSearch...

Gene expression databases

BgeeiO09043.
CleanExiMM_NAPSA.
ExpressionAtlasiO09043. baseline and differential.
GenevisibleiO09043. MM.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a novel mouse aspartic protease-like protein that is expressed abundantly in the kidney."
    Mori K., Ogawa Y., Tamura N., Ebihara K., Aoki T., Muro S., Ozaki S., Tanaka I., Tashiro K., Nakao K.
    FEBS Lett. 401:218-222(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: BALB/cJ.
    Tissue: Kidney.
  2. "Molecular organization, expression and chromosomal localization of the mouse pronapsin gene."
    Tatnell P.J., Cook M., Peters C., Kay J.
    Eur. J. Biochem. 267:6921-6930(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  4. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-128.
    Strain: C57BL/6J.
    Tissue: Plasma.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Lung.

Entry informationi

Entry nameiNAPSA_MOUSE
AccessioniPrimary (citable) accession number: O09043
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 1, 1997
Last modified: June 8, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.