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Protein

Napsin-A

Gene

Napsa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in processing of pneumocyte surfactant precursors.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei91PROSITE-ProRule annotation1
Active sitei278PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: GO_Central
  • endopeptidase activity Source: UniProtKB
  • peptidase activity Source: MGI

GO - Biological processi

  • membrane protein proteolysis Source: UniProtKB
  • protein catabolic process Source: GO_Central
  • surfactant homeostasis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.23.B1. 3474.
ReactomeiR-MMU-5683826. Surfactant metabolism.

Protein family/group databases

MEROPSiA01.046.

Names & Taxonomyi

Protein namesi
Recommended name:
Napsin-A (EC:3.4.23.-)
Alternative name(s):
KDAP-1
Kidney-derived aspartic protease-like protein
Short name:
KAP
Gene namesi
Name:Napsa
Synonyms:Kdap, Nap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:109365. Napsa.

Subcellular locationi

GO - Cellular componenti

  • alveolar lamellar body Source: UniProtKB
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • lysosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000025999? – 419Napsin-A
Signal peptidei1 – 16Sequence analysisAdd BLAST16
PropeptideiPRO_000002599817 – ?Activation peptideSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi85N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi104 ↔ 111By similarity
Glycosylationi128N-linked (GlcNAc...)1 Publication1
Glycosylationi149N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi269 ↔ 273By similarity
Disulfide bondi312 ↔ 349By similarity
Glycosylationi331N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiO09043.
PaxDbiO09043.
PeptideAtlasiO09043.
PRIDEiO09043.

PTM databases

PhosphoSitePlusiO09043.

Expressioni

Tissue specificityi

Expressed at the highest levels in the kidney, at a moderate level in the lung, and at low levels in the spleen and adipose tissue.1 Publication

Gene expression databases

BgeeiENSMUSG00000002204.
CleanExiMM_NAPSA.
ExpressionAtlasiO09043. baseline and differential.
GenevisibleiO09043. MM.

Interactioni

Protein-protein interaction databases

IntActiO09043. 3 interactors.
MINTiMINT-4103045.
STRINGi10090.ENSMUSP00000002274.

Structurei

3D structure databases

ProteinModelPortaliO09043.
SMRiO09043.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 394Peptidase A1PROSITE-ProRule annotationAdd BLAST322

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiO09043.
KOiK08565.
OMAiIILCSEI.
OrthoDBiEOG091G0JP7.
PhylomeDBiO09043.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O09043-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPLLLLLLC LLLGNLEPEE AKLIRVPLQR IHLGHRILNP LNGWEQLAEL
60 70 80 90 100
SRTSTSGGNP SFVPLSKFMN TQYFGTIGLG TPPQNFTVVF DTGSSNLWVP
110 120 130 140 150
STRCHFFSLA CWFHHRFNPK ASSSFRPNGT KFAIQYGTGR LSGILSQDNL
160 170 180 190 200
TIGGIHDAFV TFGEALWEPS LIFALAHFDG ILGLGFPTLA VGGVQPPLDA
210 220 230 240 250
MVEQGLLEKP VFSFYLNRDS EGSDGGELVL GGSDPAHYVP PLTFIPVTIP
260 270 280 290 300
AYWQVHMESV KVGTGLSLCA QGCSAILDTG TSLITGPSEE IRALNKAIGG
310 320 330 340 350
YPFLNGQYFI QCSKTPTLPP VSFHLGGVWF NLTGQDYVIK ILQSDVGLCL
360 370 380 390 400
LGFQALDIPK PAGPLWILGD VFLGPYVAVF DRGDKNVGPR VGLARAQSRS
410
TDRAERRTTQ AQFFKRRPG
Length:419
Mass (Da):45,544
Last modified:July 1, 1997 - v1
Checksum:i715ED4532791143E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88899 mRNA. Translation: BAA19004.1.
AJ250718, AJ250719, AJ250720 Genomic DNA. Translation: CAB82907.1.
BC014813 mRNA. Translation: AAH14813.1.
CCDSiCCDS21212.1.
RefSeqiNP_032463.1. NM_008437.1.
UniGeneiMm.383181.

Genome annotation databases

EnsembliENSMUST00000002274; ENSMUSP00000002274; ENSMUSG00000002204.
GeneIDi16541.
KEGGimmu:16541.
UCSCiuc009gqe.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88899 mRNA. Translation: BAA19004.1.
AJ250718, AJ250719, AJ250720 Genomic DNA. Translation: CAB82907.1.
BC014813 mRNA. Translation: AAH14813.1.
CCDSiCCDS21212.1.
RefSeqiNP_032463.1. NM_008437.1.
UniGeneiMm.383181.

3D structure databases

ProteinModelPortaliO09043.
SMRiO09043.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO09043. 3 interactors.
MINTiMINT-4103045.
STRINGi10090.ENSMUSP00000002274.

Protein family/group databases

MEROPSiA01.046.

PTM databases

PhosphoSitePlusiO09043.

Proteomic databases

EPDiO09043.
PaxDbiO09043.
PeptideAtlasiO09043.
PRIDEiO09043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002274; ENSMUSP00000002274; ENSMUSG00000002204.
GeneIDi16541.
KEGGimmu:16541.
UCSCiuc009gqe.1. mouse.

Organism-specific databases

CTDi9476.
MGIiMGI:109365. Napsa.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiO09043.
KOiK08565.
OMAiIILCSEI.
OrthoDBiEOG091G0JP7.
PhylomeDBiO09043.
TreeFamiTF314990.

Enzyme and pathway databases

BRENDAi3.4.23.B1. 3474.
ReactomeiR-MMU-5683826. Surfactant metabolism.

Miscellaneous databases

ChiTaRSiNapsa. mouse.
PROiO09043.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002204.
CleanExiMM_NAPSA.
ExpressionAtlasiO09043. baseline and differential.
GenevisibleiO09043. MM.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAPSA_MOUSE
AccessioniPrimary (citable) accession number: O09043
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.