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O09027

- ACKR2_RAT

UniProt

O09027 - ACKR2_RAT

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Protein

Atypical chemokine receptor 2

Gene

Ackr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines including CCL2, CCL3, CCL3L1, CCL4, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL22, CCL23, CCL24, SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3. Upon active ligand stimulation, activates a beta-arrestin 1 (ARRB1)-dependent, G protein-independent signaling pathway that results in the phosphorylation of the actin-binding protein cofilin (CFL1) through a RAC1-PAK1-LIMK1 signaling pathway. Activation of this pathway results in up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. By scavenging chemokines in tissues, on the surfaces of lymphatic vessels, and in placenta, plays an essential role in the resolution (termination) of the inflammatory response and in the regulation of adaptive immune responses. Plays a major role in the immune silencing of macrophages during the resolution of inflammation. Acts as a regulator of inflammatory leukocyte interactions with lymphatic endothelial cells (LECs) and is required for immature/mature dendritic cells discrimination by LECs.

GO - Molecular functioni

  1. C-X-C chemokine receptor activity Source: InterPro
  2. scavenger receptor activity Source: UniProtKB

GO - Biological processi

  1. chemotaxis Source: InterPro
  2. inflammatory response Source: UniProtKB-KW
  3. neutrophil activation Source: InterPro
  4. receptor-mediated endocytosis Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Inflammatory response

Names & Taxonomyi

Protein namesi
Recommended name:
Atypical chemokine receptor 2
Alternative name(s):
C-C chemokine receptor D6
CCR10-related receptor
Chemokine-binding protein 2
Chemokine-binding protein D6
Gene namesi
Name:Ackr2
Synonyms:Ccbp2, Ccr10rr, D6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620323. Ackr2.

Subcellular locationi

Early endosome By similarity. Recycling endosome By similarity. Cell membrane; Multi-pass membrane protein
Note: Predominantly localizes to endocytic vesicles, and upon stimulation by the ligand is internalized via clathrin-coated pits. Once internalized, the ligand dissociates from the receptor, and is targeted to degradation while the receptor is recycled back to the cell membrane (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4949ExtracellularSequence AnalysisAdd
BLAST
Transmembranei50 – 7021Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini71 – 9222CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei93 – 11321Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini114 – 1185ExtracellularSequence Analysis
Transmembranei119 – 14022Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini141 – 16222CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei163 – 18321Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini184 – 21734ExtracellularSequence AnalysisAdd
BLAST
Transmembranei218 – 23821Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini239 – 25012CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei251 – 27121Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini272 – 29322ExtracellularSequence AnalysisAdd
BLAST
Transmembranei294 – 31421Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini315 – 38268CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. actin filament Source: UniProtKB
  2. endosome Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Atypical chemokine receptor 2PRO_0000069221Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi17 – 171N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi117 ↔ 195PROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated on serine residues in the C-terminal cytoplasmic tail.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

PTM databases

PhosphoSiteiO09027.

Expressioni

Tissue specificityi

Predominantly expressed in spleen.

Gene expression databases

GenevestigatoriO09027.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026343.

Structurei

3D structure databases

ProteinModelPortaliO09027.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni327 – 38256C-terminal cytoplasmic tailBy similarityAdd
BLAST

Domaini

The C-terminal cytoplasmic tail controls its phosphorylation, stability, intracellular trafficking itinerary, and chemokine scavenging properties.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Atypical chemokine receptor subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG297330.
HOGENOMiHOG000234122.
HOVERGENiHBG106917.
InParanoidiO09027.
PhylomeDBiO09027.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR001277. Chemokine_CXCR4.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24227. PTHR24227. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00657. CCCHEMOKINER.
PR00645. CXCCHMKINER4.
PR00237. GPCRRHODOPSN.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O09027-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPTIASPLPL ATTGPENGSS IYDYDYLDDV TVLVCSKDEV LSFGRVFLPV
60 70 80 90 100
VYSLIFVLGL AGNLLLLVVL LHSVPQRRRM IELYLLNLAV SNLLFVVTMP
110 120 130 140 150
FWAISVAWHW VFGSFLCKVV STLYSINFYC GIFFITCMSL DKYLEIVHAQ
160 170 180 190 200
PLHRPKTRFR NLLLIVMVWI TALAVSVPEM VFVKVHQTLD GVWHCYADFG
210 220 230 240 250
GHATIWKLYL RFQMNLLGFL FPLLAMIFFY SRIGCVLVRL RPPGQGRALR
260 270 280 290 300
MAAALVVVFF LLWFPYNLTL FLHSLLDLHV FGNCKISHRL DYMLQVTESL
310 320 330 340 350
AFSHCCFTPV LYAFSSHSFR QYLKAVLSVV LRRHQAPGTA HAPPCSHSES
360 370 380
SRVTAQEDVV SMNDLGERQA DISLNKGEIG NN
Length:382
Mass (Da):43,293
Last modified:July 1, 1997 - v1
Checksum:iE8714CE23DB96772
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92803 mRNA. Translation: AAB61572.1.
UniGeneiRn.10771.
Rn.234526.

Genome annotation databases

UCSCiRGD:620323. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92803 mRNA. Translation: AAB61572.1 .
UniGenei Rn.10771.
Rn.234526.

3D structure databases

ProteinModelPortali O09027.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000026343.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei O09027.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:620323. rat.

Organism-specific databases

RGDi 620323. Ackr2.

Phylogenomic databases

eggNOGi NOG297330.
HOGENOMi HOG000234122.
HOVERGENi HBG106917.
InParanoidi O09027.
PhylomeDBi O09027.

Miscellaneous databases

PROi O09027.

Gene expression databases

Genevestigatori O09027.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR001277. Chemokine_CXCR4.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view ]
PANTHERi PTHR24227. PTHR24227. 1 hit.
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00657. CCCHEMOKINER.
PR00645. CXCCHMKINER4.
PR00237. GPCRRHODOPSN.
PROSITEi PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of a novel rat CC-chemokine receptor (rCCR10rR) that binds MCP-1 and MIP-1beta with high affinity."
    Bonini J.A., Steiner D.F.
    DNA Cell Biol. 16:1023-1030(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiACKR2_RAT
AccessioniPrimary (citable) accession number: O09027
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: July 1, 1997
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3