Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O09027 (ACKR2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Atypical chemokine receptor 2
Alternative name(s):
C-C chemokine receptor D6
CCR10-related receptor
Chemokine-binding protein 2
Chemokine-binding protein D6
Gene names
Name:Ackr2
Synonyms:Ccbp2, Ccr10rr, D6
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines including CCL2, CCL3, CCL3L1, CCL4, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL22, CCL23, CCL24, SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3. Upon active ligand stimulation, activates a beta-arrestin 1 (ARRB1)-dependent, G protein-independent signaling pathway that results in the phosphorylation of the actin-binding protein cofilin (CFL1) through a RAC1-PAK1-LIMK1 signaling pathway. Activation of this pathway results in up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. By scavenging chemokines in tissues, on the surfaces of lymphatic vessels, and in placenta, plays an essential role in the resolution (termination) of the inflammatory response and in the regulation of adaptive immune responses. Plays a major role in the immune silencing of macrophages during the resolution of inflammation. Acts as a regulator of inflammatory leukocyte interactions with lymphatic endothelial cells (LECs) and is required for immature/mature dendritic cells discrimination by LECs.

Subcellular location

Early endosome By similarity. Recycling endosome By similarity. Cell membrane; Multi-pass membrane protein. Note: Predominantly localizes to endocytic vesicles, and upon stimulation by the ligand is internalized via clathrin-coated pits. Once internalized, the ligand dissociates from the receptor, and is targeted to degradation while the receptor is recycled back to the cell membrane By similarity.

Tissue specificity

Predominantly expressed in spleen.

Domain

The C-terminal cytoplasmic tail controls its phosphorylation, stability, intracellular trafficking itinerary, and chemokine scavenging properties By similarity.

Post-translational modification

Phosphorylated on serine residues in the C-terminal cytoplasmic tail By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Atypical chemokine receptor subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Atypical chemokine receptor 2
PRO_0000069221

Regions

Topological domain1 – 4949Extracellular Potential
Transmembrane50 – 7021Helical; Name=1; Potential
Topological domain71 – 9222Cytoplasmic Potential
Transmembrane93 – 11321Helical; Name=2; Potential
Topological domain114 – 1185Extracellular Potential
Transmembrane119 – 14022Helical; Name=3; Potential
Topological domain141 – 16222Cytoplasmic Potential
Transmembrane163 – 18321Helical; Name=4; Potential
Topological domain184 – 21734Extracellular Potential
Transmembrane218 – 23821Helical; Name=5; Potential
Topological domain239 – 25012Cytoplasmic Potential
Transmembrane251 – 27121Helical; Name=6; Potential
Topological domain272 – 29322Extracellular Potential
Transmembrane294 – 31421Helical; Name=7; Potential
Topological domain315 – 38268Cytoplasmic Potential
Region327 – 38256C-terminal cytoplasmic tail By similarity

Amino acid modifications

Glycosylation171N-linked (GlcNAc...) Potential
Disulfide bond117 ↔ 195 By similarity

Sequences

Sequence LengthMass (Da)Tools
O09027 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: E8714CE23DB96772

FASTA38243,293
        10         20         30         40         50         60 
MPTIASPLPL ATTGPENGSS IYDYDYLDDV TVLVCSKDEV LSFGRVFLPV VYSLIFVLGL 

        70         80         90        100        110        120 
AGNLLLLVVL LHSVPQRRRM IELYLLNLAV SNLLFVVTMP FWAISVAWHW VFGSFLCKVV 

       130        140        150        160        170        180 
STLYSINFYC GIFFITCMSL DKYLEIVHAQ PLHRPKTRFR NLLLIVMVWI TALAVSVPEM 

       190        200        210        220        230        240 
VFVKVHQTLD GVWHCYADFG GHATIWKLYL RFQMNLLGFL FPLLAMIFFY SRIGCVLVRL 

       250        260        270        280        290        300 
RPPGQGRALR MAAALVVVFF LLWFPYNLTL FLHSLLDLHV FGNCKISHRL DYMLQVTESL 

       310        320        330        340        350        360 
AFSHCCFTPV LYAFSSHSFR QYLKAVLSVV LRRHQAPGTA HAPPCSHSES SRVTAQEDVV 

       370        380 
SMNDLGERQA DISLNKGEIG NN 

« Hide

References

[1]"Molecular cloning and expression of a novel rat CC-chemokine receptor (rCCR10rR) that binds MCP-1 and MIP-1beta with high affinity."
Bonini J.A., Steiner D.F.
DNA Cell Biol. 16:1023-1030(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U92803 mRNA. Translation: AAB61572.1.
UniGeneRn.10771.
Rn.234526.

3D structure databases

ProteinModelPortalO09027.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000026343.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteO09027.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:620323. rat.

Organism-specific databases

RGD620323. Ackr2.

Phylogenomic databases

eggNOGNOG297330.
HOGENOMHOG000234122.
HOVERGENHBG106917.
InParanoidO09027.
PhylomeDBO09027.

Gene expression databases

GenevestigatorO09027.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR001277. Chemokine_CXCR4.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24227. PTHR24227. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00657. CCCHEMOKINER.
PR00645. CXCCHMKINER4.
PR00237. GPCRRHODOPSN.
PROSITEPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROO09027.

Entry information

Entry nameACKR2_RAT
AccessionPrimary (citable) accession number: O09027
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries