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Protein

Solute carrier family 15 member 4

Gene

Slc15a4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Proton oligopeptide cotransporter. Transports free histidine and certain di- and tripeptides.1 Publication

Kineticsi

  1. KM=17 µM for histidine (at pH 5.5)1 Publication

    GO - Molecular functioni

    • L-histidine transmembrane transporter activity Source: RGD
    • symporter activity Source: UniProtKB-KW

    GO - Biological processi

    • histidine transport Source: RGD
    • L-histidine transmembrane transport Source: GOC
    • oligopeptide transport Source: InterPro
    • protein transport Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Biological processi

    Peptide transport, Protein transport, Symport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Solute carrier family 15 member 4
    Alternative name(s):
    Peptide transporter 4
    Peptide/histidine transporter 1
    Short name:
    rPHT1
    Gene namesi
    Name:Slc15a4
    Synonyms:Pht1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Unplaced

    Organism-specific databases

    RGDi708469. Slc15a4.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei40 – 6021HelicalSequence analysisAdd
    BLAST
    Transmembranei67 – 8721HelicalSequence analysisAdd
    BLAST
    Transmembranei96 – 11621HelicalSequence analysisAdd
    BLAST
    Transmembranei159 – 17921HelicalSequence analysisAdd
    BLAST
    Transmembranei195 – 21521HelicalSequence analysisAdd
    BLAST
    Transmembranei223 – 24321HelicalSequence analysisAdd
    BLAST
    Transmembranei317 – 33721HelicalSequence analysisAdd
    BLAST
    Transmembranei362 – 38221HelicalSequence analysisAdd
    BLAST
    Transmembranei404 – 42421HelicalSequence analysisAdd
    BLAST
    Transmembranei458 – 47821HelicalSequence analysisAdd
    BLAST
    Transmembranei489 – 50921HelicalSequence analysisAdd
    BLAST
    Transmembranei534 – 55421HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 572572Solute carrier family 15 member 4PRO_0000338601Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei279 – 2791PhosphoserineBy similarity
    Modified residuei297 – 2971PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO09014.
    PRIDEiO09014.

    Expressioni

    Tissue specificityi

    Widely expressed in the gastrointestinal tract. Highly expressed in the brain and eye, and weakly in the lung and spleen. In brain highly expressed in the hippocampus, cerebellum and pontine nucleus, and weakly in other regions of the brain, including the cerebral cortex, brain stem, thalamus and hypothalamus.2 Publications

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000001277.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000143436.
    HOVERGENiHBG108431.
    InParanoidiO09014.
    KOiK14638.
    OrthoDBiEOG7FNC8C.
    PhylomeDBiO09014.
    TreeFamiTF330897.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    IPR018456. PTR2_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 2 hits.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 3 hits.
    PROSITEiPS01023. PTR2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O09014-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEGERAPLLG SRRAAAAAGV FAGRRAACGA VLLAELLERA AFYGVTANLV
    60 70 80 90 100
    LFLNGAPFNW EGAQASQALL LFMGLTYLGS PFGGWLADAR LGRARAILLS
    110 120 130 140 150
    LALYLLGMLA FPLLAAPRSR SFLCGDPHPE LVRNCSAPFP NGTAVCPDAA
    160 170 180 190 200
    ARRCAPATFA GLVLVGLGVA TVKANITPFG ADQVKDRGPE ATRRFFNWFY
    210 220 230 240 250
    WSINLGAILS LGGIAYIQQN VSFLTGYLIP TVCVAIAFLV FLCGQSVFIT
    260 270 280 290 300
    KPPDGSAFTD MFRILTYSCC SQRGGQRRSG EGLGVFQQSS KHSLFDSCKM
    310 320 330 340 350
    SRGGPFTEDK VEDVKALVKI VPVFLALIPY WTVYFQMQTT YVLQSLHLKI
    360 370 380 390 400
    PEISSITTTH HTLPAAWLTM FDAVLILLLI PLKDKLVDPV LRRHGLLPSS
    410 420 430 440 450
    LKRIAVGMFF VTCSAFAAGI LESKRLDLVK EKTINQTIGG VVYHAADLPI
    460 470 480 490 500
    WWQIPQYVLI GISEIFASIA GLEFAYSAAP KSMQSAIMGL FFFFSGIGSF
    510 520 530 540 550
    VGSGLLALVS LKAIGWMSSH TDFGNINSCH LHYYFFLLAA IQGATLLLFL
    560 570
    IVSVKYDRQR ARTDGGTAST RT
    Length:572
    Mass (Da):61,949
    Last modified:July 1, 1997 - v1
    Checksum:i8471EE4967E2987B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti302 – 3021R → H in AAH87709 (PubMed:15489334).Curated
    Sequence conflicti307 – 3071T → I in AAH87709 (PubMed:15489334).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB000280 mRNA. Translation: BAA20489.1.
    BC087709 mRNA. Translation: AAH87709.1.
    RefSeqiNP_653359.1. NM_144758.1.
    UniGeneiRn.10770.

    Genome annotation databases

    GeneIDi246280.
    KEGGirno:246280.
    UCSCiRGD:708469. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB000280 mRNA. Translation: BAA20489.1.
    BC087709 mRNA. Translation: AAH87709.1.
    RefSeqiNP_653359.1. NM_144758.1.
    UniGeneiRn.10770.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000001277.

    Proteomic databases

    PaxDbiO09014.
    PRIDEiO09014.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi246280.
    KEGGirno:246280.
    UCSCiRGD:708469. rat.

    Organism-specific databases

    CTDi121260.
    RGDi708469. Slc15a4.

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000143436.
    HOVERGENiHBG108431.
    InParanoidiO09014.
    KOiK14638.
    OrthoDBiEOG7FNC8C.
    PhylomeDBiO09014.
    TreeFamiTF330897.

    Miscellaneous databases

    NextBioi623657.
    PROiO09014.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    IPR018456. PTR2_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 2 hits.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 3 hits.
    PROSITEiPS01023. PTR2_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning and functional expression of a brain peptide/histidine transporter."
      Yamashita T., Shimada S., Guo W., Sato K., Kohmura E., Hayakawa T., Takagi T., Tohyama M.
      J. Biol. Chem. 272:10205-10211(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "Spatial expression patterns of peptide transporters in the human and rat gastrointestinal tracts, Caco-2 in vitro cell culture model, and multiple human tissues."
      Herrera-Ruiz D., Wang Q., Gudmundsson O.S., Cook T.J., Smith R.L., Faria T.N., Knipp G.T.
      AAPS PharmSci 3:E9-E9(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiS15A4_RAT
    AccessioniPrimary (citable) accession number: O09014
    Secondary accession number(s): Q5M931
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: July 1, 1997
    Last modified: December 9, 2015
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.