O09012 (PEX5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal targeting signal 1 receptor
Short name=PTS1 receptor
Short name=PTS1R

Alternative name(s):
PTS1-BP
PXR1P
Peroxin-5
Peroxisomal C-terminal targeting signal import receptor
Peroxisome receptor 1

Gene names

Name:	Pex5
Synonyms:	Pxr1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.
Subunit structure	Interacts with PEX7, PEX12, PEX13 and PEX14. Interacts (Cys-linked ubiquitinated) with ZFAND6 By similarity.
Subcellular location	Cytoplasm. Peroxisome membrane; Peripheral membrane protein. Note: Its distribution appears to be dynamic. It is probably a cycling receptor found mainly in the cytoplasm and as well associated to the peroxisomal membrane through a docking factor (PEX13).
Sequence similarities	Belongs to the peroxisomal targeting signal receptor family. Contains 7 TPR repeats.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Membrane Peroxisome
Coding sequence diversity	Alternative splicing
Domain	Repeat TPR repeat
PTM	Thioester bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell development Inferred from mutant phenotype Ref.1. Source: MGI cerebral cortex cell migration Inferred from mutant phenotype PubMed 10653000Ref.1. Source: MGI cerebral cortex neuron differentiation Inferred from mutant phenotype Ref.1. Source: MGI endoplasmic reticulum organization Inferred from mutant phenotype PubMed 15732085. Source: MGI fatty acid beta-oxidation Inferred from mutant phenotype PubMed 17442273Ref.1. Source: MGI mitochondrial membrane organization Inferred from mutant phenotype PubMed 11583975. Source: MGI negative regulation of protein homotetramerization Inferred from electronic annotation. Source: Compara neuromuscular process Inferred from mutant phenotype Ref.1. Source: MGI neuron migration Inferred from mutant phenotype PubMed 10653000PubMed 14586000Ref.1. Source: MGI positive regulation of multicellular organism growth Inferred from mutant phenotype PubMed 10653000Ref.1. Source: MGI protein import into peroxisome matrix, docking Inferred from Biological aspect of Ancestor. Source: RefGenome protein import into peroxisome matrix, translocation Inferred from electronic annotation. Source: Compara protein import into peroxisome membrane Inferred from electronic annotation. Source: Compara protein targeting to peroxisome Inferred from sequence or structural similarity PubMed 9668159. Source: UniProtKB protein tetramerization Inferred from electronic annotation. Source: Compara very long-chain fatty acid metabolic process Inferred from mutant phenotype PubMed 10653000. Source: MGI
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara cytosol Inferred from Biological aspect of Ancestor. Source: RefGenome peroxisomal matrix Inferred from electronic annotation. Source: Compara peroxisomal membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	peroxisome matrix targeting signal-1 binding Inferred from Biological aspect of Ancestor. Source: RefGenome protein C-terminus binding Inferred from sequence or structural similarity PubMed 9668159. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O09012-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O09012-2)

The sequence of this isoform differs from the canonical sequence as follows:
     216-252: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 639	639	Peroxisomal targeting signal 1 receptor		PRO_0000106306
Regions
	Repeat	337 – 370	34	TPR 1
	Repeat	371 – 404	34	TPR 2
	Repeat	405 – 438	34	TPR 3
	Repeat	452 – 485	34	TPR 4
	Repeat	488 – 521	34	TPR 5
	Repeat	522 – 555	34	TPR 6
	Repeat	556 – 589	34	TPR 7
Amino acid modifications
	Cross-link	11		Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Natural variations
	Alternative sequence	216 – 252	37	Missing in isoform 2.		VSP_024107
Experimental info
	Sequence conflict	139	1	D → H in CAB09694. Ref.1
	Sequence conflict	150	1	D → G in CAB09694. Ref.1
	Sequence conflict	179	1	S → F in CAB09694. Ref.1
	Sequence conflict	207	1	D → N in CAB09694. Ref.1
	Sequence conflict	366	1	Q → R in CAB09694. Ref.1
	Sequence conflict	370	1	H → N in CAB09694. Ref.1
	Sequence conflict	376	1	Y → S in CAB09694. Ref.1
	Sequence conflict	473	1	D → E in BAE26240. Ref.2
	Sequence conflict	524	1	M → L in BAE26240. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) [UniParc]. Last modified April 3, 2007. Version 2. Checksum: A7013FF02926803C	FASTA	639	70,756
	10 20 30 40 50 60 MAMRELVEGE CGGANPLMKL ATHFTQDKAL RQEGLRPGPW PPGASAAETV SKPLGVGTED 70 80 90 100 110 120 ELVSEFLQDQ NATLVSRAPQ TFKMDDLLAE MQEIEQSNFR QAPQRAPGVA DLALSENWAQ 130 140 150 160 170 180 EFLAAGDAVD VAQDYNETDW SQEFIAEVTD PLSVSPARWA EEYLEQSEEK LWLGDQEGSS 190 200 210 220 230 240 TADRWYDEYH PEEDLQHTAS DFVSKVDDPK LANSEFLKFV RQIGEGQVSL ESAAGSGGAQ 250 260 270 280 290 300 AEQWAAEFIQ QQGTSEAWVD QFTRPGNKIA ALQVEFERAK SAIESDVDFW DKLQAELEEM 310 320 330 340 350 360 AKRDAEAHPW LSDYDDLTSA SYDKGYQFEE ENPLRDHPQP FEEGLHRLEE GDLPNAVLLF 370 380 390 400 410 420 EAAVQQDPKH MEAWQYLGTT QAENEQELLA ISALRRCLEL KPDNRTALMA LAVSFTNESL 430 440 450 460 470 480 QRQACETLRD WLRYSPAYAH LVAPGEEGAT GAGPSKRILG SLLSDSLFLE VKDLFLAAVR 490 500 510 520 530 540 LDPTSIDPDV QCGLGVLFNL SGEYDKAVDC FTAALSVRPN DYLMWNKLGA TLANGNQSEE 550 560 570 580 590 600 AVAAYRRALE LQPGYIRSRY NLGISCINLG AHREAVEHFL EALNMQRKSR GPRGEGGAMS 610 620 630 ENIWSTLRLA LSMLGQSDAY GAADARDLSA LLAMFGLPQ
	Isoform 2 [UniParc]. Checksum: 33C7527C14BB1FE4	FASTA	602	66,806
	10 20 30 40 50 60 MAMRELVEGE CGGANPLMKL ATHFTQDKAL RQEGLRPGPW PPGASAAETV SKPLGVGTED 70 80 90 100 110 120 ELVSEFLQDQ NATLVSRAPQ TFKMDDLLAE MQEIEQSNFR QAPQRAPGVA DLALSENWAQ 130 140 150 160 170 180 EFLAAGDAVD VAQDYNETDW SQEFIAEVTD PLSVSPARWA EEYLEQSEEK LWLGDQEGSS 190 200 210 220 230 240 TADRWYDEYH PEEDLQHTAS DFVSKVDDPK LANSEGTSEA WVDQFTRPGN KIAALQVEFE 250 260 270 280 290 300 RAKSAIESDV DFWDKLQAEL EEMAKRDAEA HPWLSDYDDL TSASYDKGYQ FEEENPLRDH 310 320 330 340 350 360 PQPFEEGLHR LEEGDLPNAV LLFEAAVQQD PKHMEAWQYL GTTQAENEQE LLAISALRRC 370 380 390 400 410 420 LELKPDNRTA LMALAVSFTN ESLQRQACET LRDWLRYSPA YAHLVAPGEE GATGAGPSKR 430 440 450 460 470 480 ILGSLLSDSL FLEVKDLFLA AVRLDPTSID PDVQCGLGVL FNLSGEYDKA VDCFTAALSV 490 500 510 520 530 540 RPNDYLMWNK LGATLANGNQ SEEAVAAYRR ALELQPGYIR SRYNLGISCI NLGAHREAVE 550 560 570 580 590 600 HFLEALNMQR KSRGPRGEGG AMSENIWSTL RLALSMLGQS DAYGAADARD LSALLAMFGL PQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A mouse model for Zellweger syndrome." Baes M.I., Gressens P., Baumgart E., Carmeliet P., Casteels M., Fransen M., Evrard P., Fahimi D., Declercq P., Collen D., Vanveldhoven P., Mannaerts G.P. Nat. Genet. 17:49-57(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Studies on mammalian peroxines." Van Veldhoven P.P. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Mammary gland.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z97018 mRNA. Translation: CAB09694.1. AJ416473 mRNA. Translation: CAC94925.1. AK088886 mRNA. Translation: BAC40632.1. AK145111 mRNA. Translation: BAE26240.1. AK145361 mRNA. Translation: BAE26388.1. AK161470 mRNA. Translation: BAE36414.1. BC029748 mRNA. Translation: AAH29748.1.
IPI	IPI00130081. IPI00788355.
RefSeq	NP_033021.2. NM_008995.2. NP_787947.1. NM_175933.2.
UniGene	Mm.22418.
3D structure databases
ProteinModelPortal	O09012.
SMR	O09012. Positions 337-639.
ModBase	Search...
Protein-protein interaction databases
IntAct	O09012. 2 interactions.
Protein family/group databases
TCDB	3.A.20.1.1. peroxisomal protein importer (PPI) family.
PTM databases
PhosphoSite	O09012.
Proteomic databases
PaxDb	O09012.
PRIDE	O09012.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000035861; ENSMUSP00000049132; ENSMUSG00000005069. ENSMUST00000080557; ENSMUSP00000079398; ENSMUSG00000005069. ENSMUST00000112531; ENSMUSP00000108150; ENSMUSG00000005069. ENSMUST00000112532; ENSMUSP00000108151; ENSMUSG00000005069.
GeneID	19305.
KEGG	mmu:19305.
UCSC	uc009dqs.1. mouse. uc009dqt.1. mouse.
Organism-specific databases
CTD	5830.
MGI	MGI:1098808. Pex5.
Phylogenomic databases
eggNOG	COG0457.
GeneTree	ENSGT00390000013941.
HOGENOM	HOG000158146.
HOVERGEN	HBG053575.
InParanoid	O09012.
KO	K13342.
OMA	LKPDNRT.
OrthoDB	EOG42V8FQ.
Gene expression databases
ArrayExpress	O09012.
Bgee	O09012.
CleanEx	MM_PEX5.
Genevestigator	O09012.
GermOnline	ENSMUSG00000005069. Mus musculus.
Family and domain databases
Gene3D	1.25.40.10. 2 hits.
InterPro	IPR024111. PTS1R_family. IPR001440. TPR-1. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
PANTHER	PTHR10130. PTHR10130. 1 hit.
Pfam	PF00515. TPR_1. 3 hits. [Graphical view]
SMART	SM00028. TPR. 4 hits. [Graphical view]
PROSITE	PS50005. TPR. 5 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	296267.
SOURCE	Search...

Entry information

Entry name

PEX5_MOUSE

Accession

Primary (citable) accession number: O09012
Secondary accession number(s): Q3UM58, Q8K2V5, Q91YC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 3, 2007
Last modified:	April 3, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program