Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O09012 (PEX5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peroxisomal targeting signal 1 receptor

Short name=PTS1 receptor
Short name=PTS1R
Alternative name(s):
PTS1-BP
PXR1P
Peroxin-5
Peroxisomal C-terminal targeting signal import receptor
Peroxisome receptor 1
Gene names
Name:Pex5
Synonyms:Pxr1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length639 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.

Subunit structure

Interacts with PEX7, PEX12, PEX13 and PEX14. Interacts (Cys-linked ubiquitinated) with ZFAND6 By similarity.

Subcellular location

Cytoplasm. Peroxisome membrane; Peripheral membrane protein. Note: Its distribution appears to be dynamic. It is probably a cycling receptor found mainly in the cytoplasm and as well associated to the peroxisomal membrane through a docking factor (PEX13).

Post-translational modification

Monoubiquitination at Cys-11 is required for proper export from peroxisomes and recycling By similarity.

Sequence similarities

Belongs to the peroxisomal targeting signal receptor family.

Contains 7 TPR repeats.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasm
Membrane
Peroxisome
   Coding sequence diversityAlternative splicing
   DomainRepeat
TPR repeat
   PTMThioester bond
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell development

Inferred from mutant phenotype Ref.1. Source: MGI

cellular lipid metabolic process

Inferred from mutant phenotype PubMed 16484321. Source: MGI

cerebral cortex cell migration

Inferred from mutant phenotype PubMed 10653000Ref.1. Source: MGI

cerebral cortex neuron differentiation

Inferred from mutant phenotype Ref.1. Source: MGI

endoplasmic reticulum organization

Inferred from mutant phenotype PubMed 15732085. Source: MGI

fatty acid beta-oxidation

Inferred from mutant phenotype PubMed 17442273Ref.1. Source: MGI

mitochondrial membrane organization

Inferred from mutant phenotype PubMed 11583975. Source: MGI

mitochondrion organization

Inferred from mutant phenotype PubMed 15732085. Source: MGI

negative regulation of protein homotetramerization

Inferred from electronic annotation. Source: Ensembl

neuromuscular process

Inferred from mutant phenotype Ref.1. Source: MGI

neuron migration

Inferred from mutant phenotype PubMed 10653000PubMed 14586000Ref.1. Source: MGI

peroxisome organization

Inferred from direct assay PubMed 14586000. Source: MGI

positive regulation of multicellular organism growth

Inferred from mutant phenotype PubMed 10653000Ref.1. Source: MGI

protein import into peroxisome matrix

Inferred from mutant phenotype PubMed 11463335. Source: UniProtKB

protein import into peroxisome matrix, docking

Inferred from Biological aspect of Ancestor. Source: RefGenome

protein import into peroxisome matrix, translocation

Inferred from electronic annotation. Source: Ensembl

protein import into peroxisome membrane

Inferred from electronic annotation. Source: Ensembl

protein targeting to peroxisome

Inferred from sequence or structural similarity PubMed 9668159. Source: UniProtKB

protein tetramerization

Inferred from electronic annotation. Source: Ensembl

very long-chain fatty acid metabolic process

Inferred from mutant phenotype PubMed 10653000. Source: MGI

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: Ensembl

cytosol

Inferred from Biological aspect of Ancestor. Source: RefGenome

mitochondrion

Inferred from mutant phenotype PubMed 11583975. Source: GOC

peroxisomal matrix

Inferred from electronic annotation. Source: Ensembl

peroxisomal membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

peroxisome

Inferred from sequence orthology PubMed 11829486. Source: MGI

protein complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionperoxisome matrix targeting signal-1 binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

peroxisome targeting sequence binding

Traceable author statement PubMed 11792727. Source: MGI

protein C-terminus binding

Inferred from sequence or structural similarity PubMed 9668159. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O09012-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O09012-2)

The sequence of this isoform differs from the canonical sequence as follows:
     216-252: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 639639Peroxisomal targeting signal 1 receptor
PRO_0000106306

Regions

Repeat337 – 37034TPR 1
Repeat371 – 40434TPR 2
Repeat405 – 43834TPR 3
Repeat452 – 48534TPR 4
Repeat488 – 52134TPR 5
Repeat522 – 55534TPR 6
Repeat556 – 58934TPR 7

Amino acid modifications

Cross-link11Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Natural variations

Alternative sequence216 – 25237Missing in isoform 2.
VSP_024107

Experimental info

Sequence conflict1391D → H in CAB09694. Ref.1
Sequence conflict1501D → G in CAB09694. Ref.1
Sequence conflict1791S → F in CAB09694. Ref.1
Sequence conflict2071D → N in CAB09694. Ref.1
Sequence conflict3661Q → R in CAB09694. Ref.1
Sequence conflict3701H → N in CAB09694. Ref.1
Sequence conflict3761Y → S in CAB09694. Ref.1
Sequence conflict4731D → E in BAE26240. Ref.2
Sequence conflict5241M → L in BAE26240. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) [UniParc].

Last modified April 3, 2007. Version 2.
Checksum: A7013FF02926803C

FASTA63970,756
        10         20         30         40         50         60 
MAMRELVEGE CGGANPLMKL ATHFTQDKAL RQEGLRPGPW PPGASAAETV SKPLGVGTED 

        70         80         90        100        110        120 
ELVSEFLQDQ NATLVSRAPQ TFKMDDLLAE MQEIEQSNFR QAPQRAPGVA DLALSENWAQ 

       130        140        150        160        170        180 
EFLAAGDAVD VAQDYNETDW SQEFIAEVTD PLSVSPARWA EEYLEQSEEK LWLGDQEGSS 

       190        200        210        220        230        240 
TADRWYDEYH PEEDLQHTAS DFVSKVDDPK LANSEFLKFV RQIGEGQVSL ESAAGSGGAQ 

       250        260        270        280        290        300 
AEQWAAEFIQ QQGTSEAWVD QFTRPGNKIA ALQVEFERAK SAIESDVDFW DKLQAELEEM 

       310        320        330        340        350        360 
AKRDAEAHPW LSDYDDLTSA SYDKGYQFEE ENPLRDHPQP FEEGLHRLEE GDLPNAVLLF 

       370        380        390        400        410        420 
EAAVQQDPKH MEAWQYLGTT QAENEQELLA ISALRRCLEL KPDNRTALMA LAVSFTNESL 

       430        440        450        460        470        480 
QRQACETLRD WLRYSPAYAH LVAPGEEGAT GAGPSKRILG SLLSDSLFLE VKDLFLAAVR 

       490        500        510        520        530        540 
LDPTSIDPDV QCGLGVLFNL SGEYDKAVDC FTAALSVRPN DYLMWNKLGA TLANGNQSEE 

       550        560        570        580        590        600 
AVAAYRRALE LQPGYIRSRY NLGISCINLG AHREAVEHFL EALNMQRKSR GPRGEGGAMS 

       610        620        630 
ENIWSTLRLA LSMLGQSDAY GAADARDLSA LLAMFGLPQ 

« Hide

Isoform 2 [UniParc].

Checksum: 33C7527C14BB1FE4
Show »

FASTA60266,806

References

« Hide 'large scale' references
[1]"A mouse model for Zellweger syndrome."
Baes M.I., Gressens P., Baumgart E., Carmeliet P., Casteels M., Fransen M., Evrard P., Fahimi D., Declercq P., Collen D., Vanveldhoven P., Mannaerts G.P.
Nat. Genet. 17:49-57(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Studies on mammalian peroxines."
Van Veldhoven P.P.
Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Mammary gland.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z97018 mRNA. Translation: CAB09694.1.
AJ416473 mRNA. Translation: CAC94925.1.
AK088886 mRNA. Translation: BAC40632.1.
AK145111 mRNA. Translation: BAE26240.1.
AK145361 mRNA. Translation: BAE26388.1.
AK161470 mRNA. Translation: BAE36414.1.
BC029748 mRNA. Translation: AAH29748.1.
CCDSCCDS20517.1. [O09012-1]
CCDS20518.1. [O09012-2]
RefSeqNP_001264259.1. NM_001277330.1. [O09012-2]
NP_001264734.1. NM_001277805.1. [O09012-1]
NP_033021.2. NM_008995.2. [O09012-1]
NP_787947.1. NM_175933.2. [O09012-2]
XP_006505821.1. XM_006505758.1. [O09012-1]
XP_006505822.1. XM_006505759.1. [O09012-1]
UniGeneMm.22418.

3D structure databases

ProteinModelPortalO09012.
SMRO09012. Positions 337-639.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO09012. 2 interactions.

Protein family/group databases

TCDB3.A.20.1.1. the peroxisomal protein importer (ppi) family.

PTM databases

PhosphoSiteO09012.

Proteomic databases

MaxQBO09012.
PaxDbO09012.
PRIDEO09012.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000035861; ENSMUSP00000049132; ENSMUSG00000005069. [O09012-1]
ENSMUST00000080557; ENSMUSP00000079398; ENSMUSG00000005069. [O09012-2]
ENSMUST00000112531; ENSMUSP00000108150; ENSMUSG00000005069. [O09012-2]
ENSMUST00000112532; ENSMUSP00000108151; ENSMUSG00000005069. [O09012-1]
GeneID19305.
KEGGmmu:19305.
UCSCuc009dqs.1. mouse. [O09012-1]
uc009dqt.1. mouse. [O09012-2]

Organism-specific databases

CTD5830.
MGIMGI:1098808. Pex5.

Phylogenomic databases

eggNOGCOG0457.
GeneTreeENSGT00390000013941.
HOGENOMHOG000158146.
HOVERGENHBG053575.
InParanoidO09012.
KOK13342.
OMADYNEADW.
OrthoDBEOG793B77.
PhylomeDBO09012.
TreeFamTF315044.

Gene expression databases

ArrayExpressO09012.
BgeeO09012.
CleanExMM_PEX5.
GenevestigatorO09012.

Family and domain databases

Gene3D1.25.40.10. 2 hits.
InterProIPR024111. PTS1R_family.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERPTHR10130. PTHR10130. 1 hit.
PfamPF00515. TPR_1. 3 hits.
[Graphical view]
SMARTSM00028. TPR. 4 hits.
[Graphical view]
PROSITEPS50005. TPR. 5 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio296267.
PROO09012.
SOURCESearch...

Entry information

Entry namePEX5_MOUSE
AccessionPrimary (citable) accession number: O09012
Secondary accession number(s): Q3UM58, Q8K2V5, Q91YC7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 3, 2007
Last modified: July 9, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot