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O09010

- LFNG_MOUSE

UniProt

O09010 - LFNG_MOUSE

Protein

Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe

Gene

Lfng

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules inhibiting Jagged mediated Notch signaling. Essential mediator of somite segmentation and patterning. During somite boundary formation, it restricts Notch activity in the presomitic mesoderm to a boundary-formig territory in the posterior half of the prospective somite. In this region, Notch function activates a set of genes that are involved in boundary formation and in anterior-posterior somite identity. Ectopically expressed in the thymus, Lfgn inhibits Notch signaling which results in inhibition of T-cell commitment and promotes B-cell development in lymphoid progenitors. May play a role in boundary formation of the enamel knot.

    Catalytic activityi

    Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.

    Cofactori

    Manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei85 – 862Cleavage; by furin-like proteaseSequence Analysis
    Binding sitei128 – 1281SubstrateBy similarity
    Binding sitei200 – 2001SubstrateBy similarity
    Metal bindingi201 – 2011ManganeseBy similarity
    Active sitei289 – 2891By similarity
    Metal bindingi313 – 3131ManganeseBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity Source: UniProt

    GO - Biological processi

    1. compartment pattern specification Source: MGI
    2. female meiotic division Source: MGI
    3. metabolic process Source: GOC
    4. ovarian follicle development Source: MGI
    5. positive regulation of Notch signaling pathway Source: MGI
    6. positive regulation of protein binding Source: MGI
    7. regulation of Notch signaling pathway Source: UniProt
    8. regulation of somitogenesis Source: MGI
    9. somitogenesis Source: MGI

    Keywords - Molecular functioni

    Developmental protein, Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196517. Pre-NOTCH Processing in Golgi.

    Protein family/group databases

    CAZyiGT31. Glycosyltransferase Family 31.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe (EC:2.4.1.222)
    Alternative name(s):
    O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
    Gene namesi
    Name:Lfng
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1095413. Lfng.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: Reactome
    2. integral component of Golgi membrane Source: InterPro

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 378378Beta-1,3-N-acetylglucosaminyltransferase lunatic fringePRO_0000219177Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi167 ↔ 178By similarity
    Disulfide bondi196 ↔ 259By similarity
    Disulfide bondi363 ↔ 372By similarity

    Post-translational modificationi

    A soluble form may be derived from the membrane form by proteolytic processing.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiO09010.

    PTM databases

    PhosphoSiteiO09010.

    Expressioni

    Tissue specificityi

    Detected at 12.5 dpc in all tissues examined with the highest level observed in adult brain and spleen. Detected in the dental epithelium.

    Developmental stagei

    Developmental protein. During segmentation it shows a cyclic transcription pattern which is under the control of Notch. Expressed in the caudal region of the presomitic mesoderm with each cycle corresponding to the formation time of one somite. In the dental epithelium it is detected at stage E13.5. The pattern of expression corresponds exactly to the formation of the enamel knot between late bud and early cap stages.4 Publications

    Gene expression databases

    ArrayExpressiO09010.
    BgeeiO09010.
    GenevestigatoriO09010.

    Structurei

    3D structure databases

    ProteinModelPortaliO09010.
    SMRiO09010. Positions 113-373.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88CytoplasmicSequence Analysis
    Topological domaini30 – 378349LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 2921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 31 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG70217.
    GeneTreeiENSGT00390000009913.
    HOGENOMiHOG000046678.
    HOVERGENiHBG007986.
    InParanoidiO09010.
    KOiK05948.
    OMAiFRSVHCH.
    OrthoDBiEOG7ZSHTD.
    PhylomeDBiO09010.
    TreeFamiTF324207.

    Family and domain databases

    InterProiIPR017374. Fringe.
    IPR003378. Fringe-like.
    [Graphical view]
    PfamiPF02434. Fringe. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O09010-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLQRCGRRLL LALVGALLAC LLVLTADPPP TPMPAERGRR ALRSLAGSSG    50
    GAPASGSRAA VDPGVLTREV HSLSEYFSLL TRARRDADPP PGVASRQGDG 100
    HPRPPAEVLS PRDVFIAVKT TRKFHRARLD LLFETWISRH KEMTFIFTDG 150
    EDEALAKLTG NVVLTNCSSA HSRQALSCKM AVEYDRFIES GKKWFCHVDD 200
    DNYVNLRALL RLLASYPHTQ DVYIGKPSLD RPIQATERIS EHKVRPVHFW 250
    FATGGAGFCI SRGLALKMGP WASGGHFMST AERIRLPDDC TIGYIVEALL 300
    GVPLIRSGLF HSHLENLQQV PTTELHEQVT LSYGMFENKR NAVHIKGPFS 350
    VEADPSRFRS VHCHLYPDTP WCPRSAIF 378
    Length:378
    Mass (Da):41,952
    Last modified:July 1, 1997 - v1
    Checksum:iFD0A02597BF9AFED
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti343 – 3431V → M in AK004642. (PubMed:16141072)Curated
    Sequence conflicti361 – 3611V → I in AK004642. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U94351 mRNA. Translation: AAC53262.1.
    AF015768 mRNA. Translation: AAB71668.1.
    AY124581 Genomic DNA. Translation: AAM93541.1.
    AK004642 mRNA. No translation available.
    AK153283 mRNA. Translation: BAE31866.1.
    CCDSiCCDS19821.1.
    RefSeqiNP_032520.1. NM_008494.3.
    UniGeneiMm.12834.

    Genome annotation databases

    EnsembliENSMUST00000031555; ENSMUSP00000031555; ENSMUSG00000029570.
    GeneIDi16848.
    KEGGimmu:16848.
    UCSCiuc009ahu.1. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    lunatic fringe gene homolog (lfng)

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U94351 mRNA. Translation: AAC53262.1 .
    AF015768 mRNA. Translation: AAB71668.1 .
    AY124581 Genomic DNA. Translation: AAM93541.1 .
    AK004642 mRNA. No translation available.
    AK153283 mRNA. Translation: BAE31866.1 .
    CCDSi CCDS19821.1.
    RefSeqi NP_032520.1. NM_008494.3.
    UniGenei Mm.12834.

    3D structure databases

    ProteinModelPortali O09010.
    SMRi O09010. Positions 113-373.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT31. Glycosyltransferase Family 31.

    PTM databases

    PhosphoSitei O09010.

    Proteomic databases

    PRIDEi O09010.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031555 ; ENSMUSP00000031555 ; ENSMUSG00000029570 .
    GeneIDi 16848.
    KEGGi mmu:16848.
    UCSCi uc009ahu.1. mouse.

    Organism-specific databases

    CTDi 3955.
    MGIi MGI:1095413. Lfng.

    Phylogenomic databases

    eggNOGi NOG70217.
    GeneTreei ENSGT00390000009913.
    HOGENOMi HOG000046678.
    HOVERGENi HBG007986.
    InParanoidi O09010.
    KOi K05948.
    OMAi FRSVHCH.
    OrthoDBi EOG7ZSHTD.
    PhylomeDBi O09010.
    TreeFami TF324207.

    Enzyme and pathway databases

    Reactomei REACT_196517. Pre-NOTCH Processing in Golgi.

    Miscellaneous databases

    NextBioi 290782.
    PROi O09010.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O09010.
    Bgeei O09010.
    Genevestigatori O09010.

    Family and domain databases

    InterProi IPR017374. Fringe.
    IPR003378. Fringe-like.
    [Graphical view ]
    Pfami PF02434. Fringe. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A family of mammalian Fringe genes implicated in boundary determination and the Notch pathway."
      Johnston S.H., Rauskolb C., Wilson R., Prabhakaran B., Irvine K.D., Vogt T.F.
      Development 124:2245-2254(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Fringe boundaries coincide with Notch-dependent patterning centres in mammals and alter Notch-dependent development in Drosophila."
      Cohen B., Bashirullah A., Dagnino L., Campbell C., Fisher W.W., Leow C.C., Whiting E., Ryan D., Zinyk D., Boulianne G., Hui C.-C., Gallie B., Phillips R.A., Lipshitz H.D., Egan S.E.
      Nat. Genet. 16:283-288(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Clock regulatory elements control cyclic expression of Lunatic fringe during somitogenesis."
      Cole S.E., Levorse J.M., Tilghman S.M., Vogt T.F.
      Dev. Cell 3:75-84(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 1-143, DEVELOPMENTAL STAGE.
      Strain: 129S6/SvEvTac.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Bone marrow and Lung.
    5. "Interaction between Notch signalling and Lunatic fringe during somite boundary formation in the mouse."
      del Barco Barrantes I., Elia A.J., Wuensch K., De Angelis M.H., Mak T.W., Rossant J., Conlon R.A., Gossler A., de la Pompa J.L.
      Curr. Biol. 9:470-480(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Lunatic fringe, FGF, and BMP regulate the Notch pathway during epithelial morphogenesis of teeth."
      Mustonen T., Tuemmers M., Mikami T., Itoh N., Zhang N., Gridley T., Thesleff I.
      Dev. Biol. 248:281-293(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. "Lunatic fringe is an essential mediator of somite segmentation and patterning."
      Evrard Y.A., Lun Y., Aulehla A., Gan L., Johnson R.L.
      Nature 394:377-381(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    8. "Genomic structure, mapping, and expression analysis of the mammalian Lunatic, Manic, and Radical fringe genes."
      Moran J.L., Johnston S.H., Rauskolb C., Bhalerao J., Bowcock A.M., Vogt T.F.
      Mamm. Genome 10:535-541(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    9. "Subversion of the T/B lineage decision in the thymus by lunatic fringe-mediated inhibition of Notch-1."
      Koch U., Lacombe T.A., Holland D., Bowman J.L., Cohen B.L., Egan S.E., Guidos C.J.
      Immunity 15:225-236(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    10. "Periodic Lunatic fringe expression is controlled during segmentation by a cyclic transcriptional enhancer responsive to notch signaling."
      Morales A.V., Yasuda Y., Ish-Horowicz D.
      Dev. Cell 3:63-74(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiLFNG_MOUSE
    AccessioniPrimary (citable) accession number: O09010
    Secondary accession number(s): Q3U659, Q8K3F1, Q9DC10
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2003
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3