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Protein

Beta-1,3-N-acetylglucosaminyltransferase radical fringe

Gene

Rfng

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. May be involved in limb formation and in neurogenesis (By similarity).By similarity

Catalytic activityi

Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751SubstrateBy similarity
Binding sitei148 – 1481SubstrateBy similarity
Metal bindingi149 – 1491ManganeseBy similarity
Active sitei238 – 2381By similarity
Metal bindingi262 – 2621ManganeseBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Glycosyltransferase, Transferase

Keywords - Biological processi

Differentiation, Neurogenesis

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1912420. Pre-NOTCH Processing in Golgi.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-N-acetylglucosaminyltransferase radical fringe (EC:2.4.1.222)
Alternative name(s):
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Gene namesi
Name:Rfng
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:894275. Rfng.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2923Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini30 – 332303LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Beta-1,3-N-acetylglucosaminyltransferase radical fringePRO_0000219186Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence analysis
Disulfide bondi115 ↔ 126By similarity
Disulfide bondi144 ↔ 208By similarity
Disulfide bondi312 ↔ 321By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO09009.
MaxQBiO09009.
PaxDbiO09009.
PRIDEiO09009.

PTM databases

iPTMnetiO09009.
PhosphoSiteiO09009.

Expressioni

Tissue specificityi

Detected in all the examined tissues (12.5 dpc). High expression found in adult brain.

Gene expression databases

BgeeiENSMUSG00000025158.
GenevisibleiO09009. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026156.

Structurei

3D structure databases

ProteinModelPortaliO09009.
SMRiO09009. Positions 56-323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
GeneTreeiENSGT00390000009913.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiO09009.
KOiK05948.
OMAiRQTFIFT.
OrthoDBiEOG091G08XO.
PhylomeDBiO09009.
TreeFamiTF324207.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.

Sequencei

Sequence statusi: Complete.

O09009-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRARRVLCR ACLALAAVLA VLLLLPLPLP LPLPRAPAPD PDRVPTRSLT
60 70 80 90 100
LEGDRLQPDD VFIAVKTTRK NHGPRLRLLL RTWISRAPRQ TFIFTDGDDP
110 120 130 140 150
ELQMLAGGRM INTNCSAVRT RQALCCKMSV EYDKFLESGR KWFCHVDDDN
160 170 180 190 200
YVNPKSLLHL LSTFSSNQDI YLGRPSLDHP IEATERVQGG GTSNTVKFWF
210 220 230 240 250
ATGGAGFCLS RGLALKMSPW ASLGSFMSTA ERVRLPDDCT VGYIVEGLLG
260 270 280 290 300
ARLLHSPLFH SHLENLQRLP SGAILQQVTL SYGGPENPHN VVNVAGSFNI
310 320 330
QQDPTRFQSV HCLLYPDTHW CPMKNRVEGA FQ
Length:332
Mass (Da):36,926
Last modified:July 1, 1997 - v1
Checksum:i4152A7547D917CA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94350 mRNA. Translation: AAC53261.1.
AF015770 mRNA. Translation: AAB71670.1.
AL663090 Genomic DNA. Translation: CAM27539.1.
BC066023 mRNA. Translation: AAH66023.1.
CCDSiCCDS25757.1.
RefSeqiNP_033079.1. NM_009053.2.
UniGeneiMm.871.

Genome annotation databases

EnsembliENSMUST00000026156; ENSMUSP00000026156; ENSMUSG00000025158.
GeneIDi19719.
KEGGimmu:19719.
UCSCiuc007mum.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94350 mRNA. Translation: AAC53261.1.
AF015770 mRNA. Translation: AAB71670.1.
AL663090 Genomic DNA. Translation: CAM27539.1.
BC066023 mRNA. Translation: AAH66023.1.
CCDSiCCDS25757.1.
RefSeqiNP_033079.1. NM_009053.2.
UniGeneiMm.871.

3D structure databases

ProteinModelPortaliO09009.
SMRiO09009. Positions 56-323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026156.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

PTM databases

iPTMnetiO09009.
PhosphoSiteiO09009.

Proteomic databases

EPDiO09009.
MaxQBiO09009.
PaxDbiO09009.
PRIDEiO09009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026156; ENSMUSP00000026156; ENSMUSG00000025158.
GeneIDi19719.
KEGGimmu:19719.
UCSCiuc007mum.1. mouse.

Organism-specific databases

CTDi5986.
MGIiMGI:894275. Rfng.

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
GeneTreeiENSGT00390000009913.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiO09009.
KOiK05948.
OMAiRQTFIFT.
OrthoDBiEOG091G08XO.
PhylomeDBiO09009.
TreeFamiTF324207.

Enzyme and pathway databases

ReactomeiR-MMU-1912420. Pre-NOTCH Processing in Golgi.

Miscellaneous databases

PROiO09009.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025158.
GenevisibleiO09009. MM.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRFNG_MOUSE
AccessioniPrimary (citable) accession number: O09009
Secondary accession number(s): B1ATU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: July 1, 1997
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.