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Protein

Beta-1,3-N-acetylglucosaminyltransferase manic fringe

Gene

Mfng

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycosyltransferase involved in the elongation of O-linked ligands to activate Notch signaling. Possesses fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity.

Catalytic activityi

Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.

Cofactori

Mn2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70Substrate1
Binding sitei143Substrate1
Metal bindingi144Manganese1
Active sitei2321 Publication1
Metal bindingi256Manganese1

GO - Molecular functioni

GO - Biological processi

  • marginal zone B cell differentiation Source: UniProtKB
  • pattern specification process Source: InterPro
  • positive regulation of Notch signaling pathway Source: MGI
  • positive regulation of protein binding Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.222. 3474.
ReactomeiR-MMU-1912420. Pre-NOTCH Processing in Golgi.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-N-acetylglucosaminyltransferase manic fringe (EC:2.4.1.222)
Alternative name(s):
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Gene namesi
Name:Mfng
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1095404. Mfng.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini28 – 321LumenalSequence analysisAdd BLAST294

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191831 – 321Beta-1,3-N-acetylglucosaminyltransferase manic fringeAdd BLAST321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi109N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi110 ↔ 1211 Publication
Disulfide bondi139 ↔ 2021 Publication
Glycosylationi185N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi306 ↔ 3151 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO09008.
PRIDEiO09008.

PTM databases

PhosphoSitePlusiO09008.

Expressioni

Gene expression databases

BgeeiENSMUSG00000018169.
ExpressionAtlasiO09008. baseline and differential.
GenevisibleiO09008. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000018313.

Structurei

Secondary structure

1321
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi53 – 55Combined sources3
Beta strandi56 – 61Combined sources6
Helixi64 – 66Combined sources3
Turni67 – 70Combined sources4
Helixi71 – 76Combined sources6
Helixi78 – 81Combined sources4
Helixi83 – 85Combined sources3
Beta strandi86 – 92Combined sources7
Helixi95 – 101Combined sources7
Helixi102 – 104Combined sources3
Beta strandi105 – 107Combined sources3
Helixi121 – 133Combined sources13
Beta strandi136 – 142Combined sources7
Beta strandi145 – 147Combined sources3
Helixi149 – 156Combined sources8
Beta strandi166 – 168Combined sources3
Helixi197 – 199Combined sources3
Beta strandi201 – 204Combined sources4
Helixi205 – 215Combined sources11
Turni224 – 228Combined sources5
Helixi231 – 241Combined sources11
Beta strandi247 – 249Combined sources3
Beta strandi256 – 258Combined sources3
Helixi260 – 262Combined sources3
Turni265 – 267Combined sources3
Helixi268 – 270Combined sources3
Beta strandi272 – 275Combined sources4
Beta strandi277 – 279Combined sources3
Beta strandi282 – 284Combined sources3
Turni294 – 296Combined sources3
Helixi301 – 308Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J0AX-ray1.80A45-321[»]
2J0BX-ray2.10A45-321[»]
ProteinModelPortaliO09008.
SMRiO09008.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO09008.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
GeneTreeiENSGT00390000009913.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiO09008.
KOiK05948.
OMAiCKMAAEL.
OrthoDBiEOG091G08XO.
PhylomeDBiO09008.
TreeFamiTF324207.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O09008-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHCRLFRGMA GALFTLLCVG LLSLRYHSSL SQRMIQGALR LNQRNPGPLE
60 70 80 90 100
LQLGDIFIAV KTTWAFHRSR LDLLLDTWVS RIRQQTFIFT DSPDERLQER
110 120 130 140 150
LGPHLVVTNC SAEHSHPALS CKMAAEFDAF LVSGLRWFCH VDDDNYVNPK
160 170 180 190 200
ALLQLLKTFP QDRDVYVGKP SLNRPIHASE LQSKNRTKLV RFWFATGGAG
210 220 230 240 250
FCINRQLALK MVPWASGSHF VDTSALIRLP DDCTVGYIIE CKLGGRLQPS
260 270 280 290 300
PLFHSHLETL QLLGAAQLPE QVTLSYGVFE GKLNVIKLPG PFSHEEDPSR
310 320
FRSLHCLLYP DTPWCPLLAA P
Length:321
Mass (Da):36,200
Last modified:July 1, 1997 - v1
Checksum:i76EBCEC2314C50CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94349 mRNA. Translation: AAC53260.1.
AF015769 mRNA. Translation: AAB71669.1.
BC010983 mRNA. Translation: AAH10983.1.
CCDSiCCDS27622.1.
RefSeqiNP_032621.1. NM_008595.2.
UniGeneiMm.149235.

Genome annotation databases

EnsembliENSMUST00000018313; ENSMUSP00000018313; ENSMUSG00000018169.
GeneIDi17305.
KEGGimmu:17305.
UCSCiuc007wri.1. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

manic fringe homolog (mfng)

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94349 mRNA. Translation: AAC53260.1.
AF015769 mRNA. Translation: AAB71669.1.
BC010983 mRNA. Translation: AAH10983.1.
CCDSiCCDS27622.1.
RefSeqiNP_032621.1. NM_008595.2.
UniGeneiMm.149235.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J0AX-ray1.80A45-321[»]
2J0BX-ray2.10A45-321[»]
ProteinModelPortaliO09008.
SMRiO09008.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000018313.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

PTM databases

PhosphoSitePlusiO09008.

Proteomic databases

PaxDbiO09008.
PRIDEiO09008.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018313; ENSMUSP00000018313; ENSMUSG00000018169.
GeneIDi17305.
KEGGimmu:17305.
UCSCiuc007wri.1. mouse.

Organism-specific databases

CTDi4242.
MGIiMGI:1095404. Mfng.

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
GeneTreeiENSGT00390000009913.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiO09008.
KOiK05948.
OMAiCKMAAEL.
OrthoDBiEOG091G08XO.
PhylomeDBiO09008.
TreeFamiTF324207.

Enzyme and pathway databases

BRENDAi2.4.1.222. 3474.
ReactomeiR-MMU-1912420. Pre-NOTCH Processing in Golgi.

Miscellaneous databases

EvolutionaryTraceiO09008.
PROiO09008.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018169.
ExpressionAtlasiO09008. baseline and differential.
GenevisibleiO09008. MM.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMFNG_MOUSE
AccessioniPrimary (citable) accession number: O09008
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.