Reviewed, UniProtKB/Swiss-Prot O09008 (MFNG_MOUSE)

Names and origin

Protein names

Recommended name:
    Beta-1,3-N-acetylglucosaminyltransferase manic fringe
    EC=2.4.1.222
Alternative name(s):
    O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Gene names

Name:

Mfng

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Glycosyltransferase involved in the elongation of O-linked ligands to activate Notch signaling. Possesses fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity.
Catalytic activity	Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 31 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Developmental protein Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	pattern specification process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 321

321

Beta-1,3-N-acetylglucosaminyltransferase manic fringe

PRO_0000219183

Regions

Topological domain

1 – 7

7

Cytoplasmic Potential

Transmembrane

8 – 27

20

Signal-anchor for type II membrane protein Potential

Topological domain

28 – 321

294

Lumenal Potential

Amino acid modifications

Glycosylation

109

1

N-linked (GlcNAc...) Potential

Glycosylation

185

1

N-linked (GlcNAc...) Potential

Secondary structure

1

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321

Helix Strand Turn

Details...

Helix

53 – 55

3

Beta strand

56 – 61

6

Helix

64 – 66

3

Turn

67 – 70

4

Helix

71 – 76

6

Helix

78 – 81

4

Helix

83 – 85

3

Beta strand

86 – 92

7

Helix

95 – 101

7

Helix

102 – 104

3

Beta strand

105 – 107

3

Helix

121 – 133

13

Beta strand

136 – 142

7

Beta strand

145 – 147

3

Helix

149 – 156

8

Beta strand

166 – 168

3

Helix

197 – 199

3

Beta strand

201 – 204

4

Helix

205 – 215

11

Turn

224 – 228

5

Helix

231 – 241

11

Beta strand

247 – 249

3

Beta strand

256 – 258

3

Helix

260 – 262

3

Turn

265 – 267

3

Helix

268 – 270

3

Beta strand

272 – 275

4

Beta strand

277 – 279

3

Beta strand

282 – 284

3

Turn

294 – 296

3

Helix

301 – 308

8

Sequences

	Sequence		Length	Mass (Da)
	O09008-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 76EBCEC2314C50CD	FASTA	321	36,200
	10 20 30 40 50 60 MHCRLFRGMA GALFTLLCVG LLSLRYHSSL SQRMIQGALR LNQRNPGPLE LQLGDIFIAV 70 80 90 100 110 120 KTTWAFHRSR LDLLLDTWVS RIRQQTFIFT DSPDERLQER LGPHLVVTNC SAEHSHPALS 130 140 150 160 170 180 CKMAAEFDAF LVSGLRWFCH VDDDNYVNPK ALLQLLKTFP QDRDVYVGKP SLNRPIHASE 190 200 210 220 230 240 LQSKNRTKLV RFWFATGGAG FCINRQLALK MVPWASGSHF VDTSALIRLP DDCTVGYIIE 250 260 270 280 290 300 CKLGGRLQPS PLFHSHLETL QLLGAAQLPE QVTLSYGVFE GKLNVIKLPG PFSHEEDPSR 310 320 FRSLHCLLYP DTPWCPLLAA P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A family of mammalian Fringe genes implicated in boundary determination and the Notch pathway." Johnston S.H., Rauskolb C., Wilson R., Prabhakaran B., Irvine K.D., Vogt T.F. Development 124:2245-2254(1997) [PubMed: 9187150] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Fringe boundaries coincide with Notch-dependent patterning centres in mammals and alter Notch-dependent development in Drosophila." Cohen B., Bashirullah A., Dagnino L., Campbell C., Fisher W.W., Leow C.C., Whiting E., Ryan D., Zinyk D., Boulianne G., Hui C.-C., Gallie B., Phillips R.A., Lipshitz H.D., Egan S.E. Nat. Genet. 16:283-288(1997) [PubMed: 9207795] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

manic fringe homolog (mfng)

Cross-references

Sequence databases

EMBLGenBankDDBJ

U94349 mRNA. Translation: AAC53260.1.
AF015769 mRNA. Translation: AAB71669.1.
BC010983 mRNA. Translation: AAH10983.1.

IPI

IPI00113736.

RefSeq

NP_032621.1.

UniGene

Mm.149235

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2J0A	X-ray	1.80	A	45-321	[»]
2J0B	X-ray	2.10	A	45-321	[»]

ModBase

Search...

Protein family/group databases

CAZy

GT31. Glycosyltransferase Family 31.

Genome annotation databases

Ensembl

ENSMUSG00000018169. Mus musculus. [Contig view]

GeneID

17305.

KEGG

mmu:17305.

Organism-specific databases

MGI

MGI:1095404. Mfng.

Phylogenomic databases

HOGENOM

O09008.

HOVERGEN

O09008.

OMA

O09008. PHNRTRL.

Enzyme and pathway databases

BRENDA

2.4.1.222. 244.

Gene expression databases

ArrayExpress

O09008.

Bgee

O09008.

GermOnline

ENSMUSG00000018169. Mus musculus.

Family and domain databases

InterPro

IPR017374. Fringe.
IPR003378. Fringe-like.
[Graphical view]

Pfam

PF02434. Fringe. 1 hit.
[Graphical view]

PIRSF

PIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

291848.

SOURCE

Search...

Entry information

Entry name

MFNG_MOUSE

Accession

Primary (citable) accession number: O09008

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)