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Protein

Sphingolipid delta(4)-desaturase DES1

Gene

Degs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has sphingolipid-delta-4-desaturase activity. Converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine).1 Publication

Catalytic activityi

A dihydroceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4E)-sphing-4-enine ceramide + 2 ferricytochrome b5 + 2 H2O.1 Publication

GO - Molecular functioni

  • sphingolipid delta-4 desaturase activity Source: MGI

GO - Biological processi

  • ceramide biosynthetic process Source: MGI
  • fatty acid biosynthetic process Source: UniProtKB-KW
  • positive regulation of apoptotic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Chemistry

SwissLipidsiSLP:000000167.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingolipid delta(4)-desaturase DES1 (EC:1.14.19.171 Publication)
Alternative name(s):
Degenerative spermatocyte homolog 1
Gene namesi
Name:Degs1
Synonyms:Degs, Des1, Mdes
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1097711. Degs1.

Subcellular locationi

  • Mitochondrion By similarity
  • Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
  • Membrane By similarity; Lipid-anchor By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei41 – 6121HelicalSequence analysisAdd
BLAST
Transmembranei68 – 8821HelicalSequence analysisAdd
BLAST
Transmembranei104 – 12421HelicalSequence analysisAdd
BLAST
Transmembranei152 – 17221HelicalSequence analysisAdd
BLAST
Transmembranei184 – 20421HelicalSequence analysisAdd
BLAST
Transmembranei209 – 22921HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 323322Sphingolipid delta(4)-desaturase DES1PRO_0000312729Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei307 – 3071PhosphoserineBy similarity

Post-translational modificationi

Myristoylation can target the enzyme to the mitochondria leading to an increase in ceramide levels.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiO09005.
MaxQBiO09005.
PaxDbiO09005.
PRIDEiO09005.

PTM databases

iPTMnetiO09005.
PhosphoSiteiO09005.
SwissPalmiO09005.

Expressioni

Tissue specificityi

Detected in testis. Detected in pachytene spermatocytes and round spermatids.1 Publication

Gene expression databases

BgeeiO09005.
CleanExiMM_DEGS1.
ExpressionAtlasiO09005. baseline and differential.
GenevisibleiO09005. MM.

Interactioni

Protein-protein interaction databases

IntActiO09005. 1 interaction.
STRINGi10090.ENSMUSP00000048519.

Structurei

3D structure databases

ProteinModelPortaliO09005.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi89 – 935Histidine box-1Curated
Motifi128 – 1325Histidine box-2Curated
Motifi259 – 2635Histidine box-3Curated

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2987. Eukaryota.
ENOG410XQVT. LUCA.
GeneTreeiENSGT00390000013448.
HOGENOMiHOG000188299.
HOVERGENiHBG052775.
InParanoidiO09005.
KOiK04712.
OMAiEFYETMP.
OrthoDBiEOG7WDN2X.
PhylomeDBiO09005.
TreeFamiTF313582.

Family and domain databases

InterProiIPR031196. DES1.
IPR011388. DES1/DES2.
IPR005804. Fatty_acid_desaturase_dom.
IPR013866. Sphingolipid_d4-desaturase_N.
[Graphical view]
PANTHERiPTHR12879:SF2. PTHR12879:SF2. 1 hit.
PfamiPF00487. FA_desaturase. 1 hit.
PF08557. Lipid_DES. 1 hit.
[Graphical view]
PIRSFiPIRSF017228. Sphnglp_dlt4_des. 1 hit.
SMARTiSM01269. Lipid_DES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O09005-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSRVSREEF EWVYTDQPHA ARRKEILAKY PEIKSLMKPD HNLIWIVAMM
60 70 80 90 100
LLVQLASFYL VKDLDWKWVI FWSYVFGSCL NHSMTLAIHE ISHNFPFGHH
110 120 130 140 150
KALWNRWFGM FANLSLGVPY SISFKRYHMD HHRYLGADKI DVDIPTDFEG
160 170 180 190 200
WFFCTTFRKF VWVILQPLFY AFRPLFINPK PITYLEIINT VIQITFDIII
210 220 230 240 250
YYVFGVKSLV YMLAATLLGL GLHPISGHFI AEHYMFLKGH ETYSYYGPLN
260 270 280 290 300
LLTFNVGYHN EHHDFPNVPG KNLPMVRKIA SEYYDDLPHY NSWIKVLYDF
310 320
VTDDTISPYS RMKRPPKGNE ILE
Length:323
Mass (Da):38,241
Last modified:July 1, 1997 - v1
Checksum:iC1840C24E6FF29E0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti203 – 2031V → A in AAM12532 (PubMed:11937514).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08460 mRNA. Translation: CAA69714.1.
AF466376 mRNA. Translation: AAM12532.1.
AK002617 mRNA. Translation: BAB22233.1.
AK077257 mRNA. Translation: BAC36713.1.
AK149811 mRNA. Translation: BAE29098.1.
AK163404 mRNA. Translation: BAE37337.1.
BC003751 mRNA. Translation: AAH03751.1.
CCDSiCCDS15587.1.
RefSeqiNP_031879.1. NM_007853.4.
UniGeneiMm.29648.

Genome annotation databases

EnsembliENSMUST00000035295; ENSMUSP00000048519; ENSMUSG00000038633.
GeneIDi13244.
KEGGimmu:13244.
UCSCiuc007dxw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08460 mRNA. Translation: CAA69714.1.
AF466376 mRNA. Translation: AAM12532.1.
AK002617 mRNA. Translation: BAB22233.1.
AK077257 mRNA. Translation: BAC36713.1.
AK149811 mRNA. Translation: BAE29098.1.
AK163404 mRNA. Translation: BAE37337.1.
BC003751 mRNA. Translation: AAH03751.1.
CCDSiCCDS15587.1.
RefSeqiNP_031879.1. NM_007853.4.
UniGeneiMm.29648.

3D structure databases

ProteinModelPortaliO09005.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO09005. 1 interaction.
STRINGi10090.ENSMUSP00000048519.

Chemistry

SwissLipidsiSLP:000000167.

PTM databases

iPTMnetiO09005.
PhosphoSiteiO09005.
SwissPalmiO09005.

Proteomic databases

EPDiO09005.
MaxQBiO09005.
PaxDbiO09005.
PRIDEiO09005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035295; ENSMUSP00000048519; ENSMUSG00000038633.
GeneIDi13244.
KEGGimmu:13244.
UCSCiuc007dxw.1. mouse.

Organism-specific databases

CTDi8560.
MGIiMGI:1097711. Degs1.

Phylogenomic databases

eggNOGiKOG2987. Eukaryota.
ENOG410XQVT. LUCA.
GeneTreeiENSGT00390000013448.
HOGENOMiHOG000188299.
HOVERGENiHBG052775.
InParanoidiO09005.
KOiK04712.
OMAiEFYETMP.
OrthoDBiEOG7WDN2X.
PhylomeDBiO09005.
TreeFamiTF313582.

Enzyme and pathway databases

ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

PROiO09005.
SOURCEiSearch...

Gene expression databases

BgeeiO09005.
CleanExiMM_DEGS1.
ExpressionAtlasiO09005. baseline and differential.
GenevisibleiO09005. MM.

Family and domain databases

InterProiIPR031196. DES1.
IPR011388. DES1/DES2.
IPR005804. Fatty_acid_desaturase_dom.
IPR013866. Sphingolipid_d4-desaturase_N.
[Graphical view]
PANTHERiPTHR12879:SF2. PTHR12879:SF2. 1 hit.
PfamiPF00487. FA_desaturase. 1 hit.
PF08557. Lipid_DES. 1 hit.
[Graphical view]
PIRSFiPIRSF017228. Sphnglp_dlt4_des. 1 hit.
SMARTiSM01269. Lipid_DES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mdes, a mouse homolog of the Drosophila degenerative spermatocyte gene is expressed during mouse spermatogenesis."
    Endo K., Matsuda Y., Kobayashi S.
    Dev. Growth Differ. 39:399-403(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: ddY.
    Tissue: Testis.
  2. "Identification and characterization of a sphingolipid delta 4-desaturase family."
    Ternes P., Franke S., Zaehringer U., Sperling P., Heinz E.
    J. Biol. Chem. 277:25512-25518(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: C57BL/6J.
    Tissue: Kidney.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow macrophage, Egg, Kidney, Ovary and Uterus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiDEGS1_MOUSE
AccessioniPrimary (citable) accession number: O09005
Secondary accession number(s): Q8R4H3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: July 1, 1997
Last modified: June 8, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.