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Protein

Cytohesin-3

Gene

Cyth3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF factors through replacement of GDP with GTP.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei284Phosphatidylinositol 3,4,5-trisphosphate2 Publications1
Binding sitei295Phosphatidylinositol 3,4,5-trisphosphate2 Publications1
Binding sitei305Phosphatidylinositol 3,4,5-trisphosphate2 Publications1
Binding sitei354Phosphatidylinositol 3,4,5-trisphosphate2 Publications1

GO - Molecular functioni

  • ARF guanyl-nucleotide exchange factor activity Source: UniProtKB
  • guanyl-nucleotide exchange factor activity Source: MGI
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: MGI

GO - Biological processi

  • establishment of epithelial cell polarity Source: MGI
  • Golgi vesicle transport Source: UniProtKB
  • positive regulation of cell adhesion Source: MGI
  • regulation of ARF protein signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytohesin-3
Alternative name(s):
ARF nucleotide-binding site opener 3
Short name:
Protein ARNO3
General receptor of phosphoinositides 1
Short name:
Grp1
PH, SEC7 and coiled-coil domain-containing protein 3
Short name:
CLM3
SEC7 homolog C
Short name:
mSec7-3
Gene namesi
Name:Cyth3
Synonyms:Grp1, Pscd3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1335107. Cyth3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB
  • extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
  • plasma membrane Source: MGI
  • ruffle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi273K → A: Abolishes phosphatidylinositol 3,4,5-trisphosphate binding. 1 Publication1
Mutagenesisi277R → A or G: Reduces phosphatidylinositol 3,4,5-trisphosphate binding. 1 Publication1
Mutagenesisi280T → A or G: Reduces phosphatidylinositol 3,4,5-trisphosphate binding. 1 Publication1
Mutagenesisi282K → A: Reduces phosphatidylinositol 3,4,5-trisphosphate binding. 1 Publication1
Mutagenesisi284R → A: Abolishes phosphatidylinositol 3,4,5-trisphosphate binding. 1 Publication1
Mutagenesisi295Y → F: Reduces phosphatidylinositol 3,4,5-trisphosphate binding. 1 Publication1
Mutagenesisi305R → A: Abolishes phosphatidylinositol 3,4,5-trisphosphate binding. 1 Publication1
Mutagenesisi343K → A: Abolishes phosphatidylinositol 3,4,5-trisphosphate binding. 1 Publication1
Mutagenesisi354N → A: Slightly reduces phosphatidylinositol 3,4,5-trisphosphate binding. 1 Publication1
Mutagenesisi355H → A: Abolishes phosphatidylinositol 3,4,5-trisphosphate binding. 1 Publication1
Mutagenesisi388L → A: Impairs autoinhibition; when associated with A-392. 1 Publication1
Mutagenesisi392K → A: Impairs autoinhibition; when associated with A-388. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001202011 – 399Cytohesin-3Add BLAST399

Proteomic databases

PaxDbiO08967.
PRIDEiO08967.

PTM databases

iPTMnetiO08967.
PhosphoSitePlusiO08967.

Expressioni

Gene expression databases

BgeeiENSMUSG00000018001.
ExpressionAtlasiO08967. baseline and differential.
GenevisibleiO08967. MM.

Interactioni

Subunit structurei

Interacts with GRASP.5 Publications

Protein-protein interaction databases

BioGridi202413. 2 interactors.
STRINGi10090.ENSMUSP00000112157.

Structurei

Secondary structure

1399
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi64 – 79Combined sources16
Helixi81 – 90Combined sources10
Helixi98 – 107Combined sources10
Helixi113 – 121Combined sources9
Helixi125 – 136Combined sources12
Helixi145 – 152Combined sources8
Helixi162 – 179Combined sources18
Beta strandi183 – 185Combined sources3
Helixi187 – 205Combined sources19
Helixi215 – 221Combined sources7
Turni222 – 225Combined sources4
Helixi233 – 245Combined sources13
Helixi258 – 260Combined sources3
Beta strandi266 – 274Combined sources9
Beta strandi276 – 278Combined sources3
Beta strandi281 – 289Combined sources9
Beta strandi292 – 298Combined sources7
Beta strandi305 – 309Combined sources5
Beta strandi314 – 318Combined sources5
Beta strandi324 – 330Combined sources7
Beta strandi332 – 334Combined sources3
Beta strandi342 – 344Combined sources3
Beta strandi350 – 352Combined sources3
Beta strandi356 – 361Combined sources6
Helixi365 – 381Combined sources17
Turni383 – 386Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FGYX-ray1.50A261-387[»]
1FGZX-ray2.05A261-387[»]
1FHWX-ray1.90A/B264-391[»]
1FHXX-ray2.50A/B264-391[»]
1U2BX-ray1.80A261-387[»]
2R09X-ray1.90A/B63-399[»]
2R0DX-ray2.04A/B63-399[»]
ProteinModelPortaliO08967.
SMRiO08967.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08967.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 206SEC7PROSITE-ProRule annotationAdd BLAST130
Domaini264 – 380PHPROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni273 – 280Phosphatidylinositol 3,4,5-trisphosphate binding8
Regioni391 – 399C-terminal autoinhibitory region9

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili14 – 61Sequence analysisAdd BLAST48

Domaini

Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate.
Autoinhibited by its C-terminal basic region.

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SEC7 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0930. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000253023.
HOVERGENiHBG002647.
InParanoidiO08967.
KOiK18441.
OMAiNLTCVEE.
OrthoDBiEOG091G0RZS.
PhylomeDBiO08967.
TreeFamiTF352091.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08967-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEGGGGEGG SVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT
60 70 80 90 100
EIDNLTSVEE SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED
110 120 130 140 150
VAQFLYKGEG LNKTVIGDYL GERDDFNIKV LQAFVELHEF ADLNLVQALR
160 170 180 190 200
QFLWSFRLPG EAQKIDRMME AFASRYCLCN PGVFQSTDTC YVLSFAIIML
210 220 230 240 250
NTSLHNHNVR DKPTAERFIT MNRGINEGGD LPEELLRNLY ESIKNEPFKI
260 270 280 290 300
PEDDGNDLTH TFFNPDREGW LLKLGGRVKT WKRRWFILTD NCLYYFEYTT
310 320 330 340 350
DKEPRGIIPL ENLSIREVED PRKPNCFELY NPSHKGQVIK ACKTEADGRV
360 370 380 390
VEGNHVVYRI SAPSPEEKEE WMKSIKASIS RDPFYDMLAT RKRRIANKK
Length:399
Mass (Da):46,280
Last modified:July 1, 1997 - v1
Checksum:i00F7FEB9C3849157
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001871 Genomic DNA. Translation: AAB60876.1.
AB013470 mRNA. Translation: BAA33433.1.
AF084221 mRNA. Translation: AAF23858.1.
BC035296 mRNA. Translation: AAH35296.2.
CCDSiCCDS19845.1.
RefSeqiNP_035312.3. NM_011182.4.
UniGeneiMm.281003.

Genome annotation databases

EnsembliENSMUST00000116456; ENSMUSP00000112157; ENSMUSG00000018001.
GeneIDi19159.
KEGGimmu:19159.
UCSCiuc009ako.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001871 Genomic DNA. Translation: AAB60876.1.
AB013470 mRNA. Translation: BAA33433.1.
AF084221 mRNA. Translation: AAF23858.1.
BC035296 mRNA. Translation: AAH35296.2.
CCDSiCCDS19845.1.
RefSeqiNP_035312.3. NM_011182.4.
UniGeneiMm.281003.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FGYX-ray1.50A261-387[»]
1FGZX-ray2.05A261-387[»]
1FHWX-ray1.90A/B264-391[»]
1FHXX-ray2.50A/B264-391[»]
1U2BX-ray1.80A261-387[»]
2R09X-ray1.90A/B63-399[»]
2R0DX-ray2.04A/B63-399[»]
ProteinModelPortaliO08967.
SMRiO08967.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202413. 2 interactors.
STRINGi10090.ENSMUSP00000112157.

PTM databases

iPTMnetiO08967.
PhosphoSitePlusiO08967.

Proteomic databases

PaxDbiO08967.
PRIDEiO08967.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000116456; ENSMUSP00000112157; ENSMUSG00000018001.
GeneIDi19159.
KEGGimmu:19159.
UCSCiuc009ako.2. mouse.

Organism-specific databases

CTDi9265.
MGIiMGI:1335107. Cyth3.

Phylogenomic databases

eggNOGiKOG0930. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000253023.
HOVERGENiHBG002647.
InParanoidiO08967.
KOiK18441.
OMAiNLTCVEE.
OrthoDBiEOG091G0RZS.
PhylomeDBiO08967.
TreeFamiTF352091.

Enzyme and pathway databases

ReactomeiR-MMU-6811438. Intra-Golgi traffic.

Miscellaneous databases

EvolutionaryTraceiO08967.
PROiO08967.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018001.
ExpressionAtlasiO08967. baseline and differential.
GenevisibleiO08967. MM.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYH3_MOUSE
AccessioniPrimary (citable) accession number: O08967
Secondary accession number(s): Q8CI93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.