Reviewed, UniProtKB/Swiss-Prot O08967 (CYH3_MOUSE)

Names and origin

Protein names

Recommended name:
    Cytohesin-3
Alternative name(s):
    PH, SEC7 and coiled-coil domain-containing protein 3
      Short name=CLM3
    SEC7 homolog C
      Short name=mSec7-3
    ARF nucleotide-binding site opener 3
      Short name=Protein ARNO3
    General receptor of phosphoinositides 1
      Short name=Grp1

Gene names

Name:	Cyth3
Synonyms:	Grp1, Pscd3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF through replacement of GDP with GTP.
Subunit structure	Binds via its PH domain to the inositol head group of phosphotidylinositol 3,4,5-triphosphate with high affinity. Interacts with GRASP. Ref.5
Sequence similarities	Contains 1 PH domain. Contains 1 SEC7 domain.

Ontologies

Keywords
Domain	Coiled coil
Molecular function	Guanine-nucleotide releasing factor
Technical term	3D-structure
Gene Ontology (GO)
Biological process	positive regulation of cell adhesion Ref.3 Inferred from direct assay. Source: MGI regulation of ARF protein signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: MGI plasma membrane Ref.3 Inferred from direct assay. Source: MGI ruffle Inferred from physical interaction. Source: MGI
Molecular function	ARF guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro phosphatidylinositol-3,4,5-trisphosphate binding Ref.1 Inferred from direct assay. Source: MGI protein binding Ref.5 Inferred from physical interaction. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 399

399

Cytohesin-3

PRO_0000120201

Regions

Domain

77 – 206

130

SEC7

Domain

264 – 380

117

PH

Coiled coil

14 – 61

48

Potential

Secondary structure

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399

Helix Strand Turn

Details...

Helix

64 – 79

16

Helix

81 – 90

10

Helix

98 – 107

10

Helix

113 – 121

9

Helix

125 – 136

12

Helix

145 – 152

8

Helix

162 – 179

18

Beta strand

183 – 185

3

Helix

187 – 205

19

Helix

215 – 221

7

Turn

222 – 225

4

Helix

233 – 245

13

Helix

258 – 260

3

Beta strand

266 – 274

9

Beta strand

276 – 278

3

Beta strand

281 – 289

9

Beta strand

292 – 298

7

Beta strand

305 – 309

5

Beta strand

314 – 318

5

Beta strand

324 – 330

7

Beta strand

332 – 334

3

Beta strand

342 – 344

3

Beta strand

350 – 352

3

Beta strand

356 – 361

6

Helix

365 – 381

17

Turn

383 – 386

4

Helix

392 – 395

4

Sequences

	Sequence		Length	Mass (Da)
	O08967-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 00F7FEB9C3849157	FASTA	399	46,280
	10 20 30 40 50 60 MDEGGGGEGG SVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT EIDNLTSVEE 70 80 90 100 110 120 SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED VAQFLYKGEG LNKTVIGDYL 130 140 150 160 170 180 GERDDFNIKV LQAFVELHEF ADLNLVQALR QFLWSFRLPG EAQKIDRMME AFASRYCLCN 190 200 210 220 230 240 PGVFQSTDTC YVLSFAIIML NTSLHNHNVR DKPTAERFIT MNRGINEGGD LPEELLRNLY 250 260 270 280 290 300 ESIKNEPFKI PEDDGNDLTH TFFNPDREGW LLKLGGRVKT WKRRWFILTD NCLYYFEYTT 310 320 330 340 350 360 DKEPRGIIPL ENLSIREVED PRKPNCFELY NPSHKGQVIK ACKTEADGRV VEGNHVVYRI 370 380 390 SAPSPEEKEE WMKSIKASIS RDPFYDMLAT RKRRIANKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Signaling by phosphoinositide-3,4,5-trisphosphate through proteins containing pleckstrin and Sec7 homology domains." Klarlund J.K., Guilherme A., Holik J.J., Virbasius J.V., Chawla A., Czech M.P. Science 275:1927-1930(1997) [PubMed: 9072969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complex regulation of multiple cytohesin-like genes in murine tissues and cells." Kim H.-S., Chen Y., Lonai P. FEBS Lett. 433:312-316(1998) [PubMed: 9744817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Anergic T lymphocytes selectively express an integrin regulatory protein of the cytohesin family." Korthauer U., Nagel W., Davis E.M., Le Beau M.M., Menon R.S., Mitchell E.O., Kozak C.A., Kolanus W., Bluestone J.A. J. Immunol. 164:308-318(2000) [PubMed: 10605025] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: NIH Swiss.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[5]	"Interaction of GRASP, a protein encoded by a novel retinoic acid-induced gene, with members of the cytohesin family of guanine nucleotide exchange factors." Nevrivy D.J., Peterson V.J., Avram D., Ishmael J.E., Hansen S.G., Dowell P., Hruby D.E., Dawson M.I., Leid M. J. Biol. Chem. 275:16827-16836(2000) [PubMed: 10828067] [Abstract] Cited for: INTERACTION WITH GRASP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF001871 Genomic DNA. Translation: AAB60876.1.
AB013470 mRNA. Translation: BAA33433.1.
AF084221 mRNA. Translation: AAF23858.1.
BC035296 mRNA. Translation: AAH35296.2.

IPI

IPI00272148.

RefSeq

NP_035312.3.

UniGene

Mm.281003

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FGY	X-ray	1.50	A	261-387	[»]
1FGZ	X-ray	2.05	A	261-387	[»]
1FHW	X-ray	1.90	A/B	264-391	[»]
1FHX	X-ray	2.50	A/B	264-391	[»]
1U2B	X-ray	1.80	A	261-387	[»]
2R09	X-ray	1.90	A/B	63-399	[»]
2R0D	X-ray	2.04	A/B	63-399	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

O08967.

PTM databases

PhosphoSite

O08967.

Proteomic databases

PRIDE

O08967.

Genome annotation databases

Ensembl

ENSMUST00000018145; ENSMUSP00000018145; ENSMUSG00000018001; Mus musculus. [Genome view]
ENSMUST00000116456; ENSMUSP00000112157; ENSMUSG00000018001; Mus musculus. [Genome view]

GeneID

19159.

Organism-specific databases

CTD

19159.

MGI

MGI:1335107. Cyth3.

Phylogenomic databases

eggNOG

roNOG08244.

HOVERGEN

O08967.

InParanoid

O08967.

OMA

WMKSIRA.

PhylomeDB

O08967.

Gene expression databases

ArrayExpress

O08967.

Bgee

O08967.

Genevestigator

O08967.

GermOnline

ENSMUSG00000018001. Mus musculus.

Family and domain databases

InterPro

IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR000904. Sec7.
[Graphical view]

Gene3D

G3DSA:2.30.29.30. PH_type. 1 hit.

Pfam

PF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]

SMART

SM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]

PROSITE

PS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

Entry information

Entry name

CYH3_MOUSE

Accession

Primary (citable) accession number: O08967
Secondary accession number(s): Q8CI93

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	July 1, 1997
Last modified:	January 19, 2010
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)