Cytohesin-3 - Mus musculus (Mouse)

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O08967 (CYH3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytohesin-3

Alternative name(s):
ARF nucleotide-binding site opener 3
Short name=Protein ARNO3
General receptor of phosphoinositides 1
Short name=Grp1
PH, SEC7 and coiled-coil domain-containing protein 3
Short name=CLM3
SEC7 homolog C
Short name=mSec7-3

Gene names

Name:	Cyth3
Synonyms:	Grp1, Pscd3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF through replacement of GDP with GTP.
Subunit structure	Binds via its PH domain to the inositol head group of phosphotidylinositol 3,4,5-triphosphate with high affinity. Interacts with GRASP. Ref.5
Subcellular location	Cell membrane By similarity.
Sequence similarities	Contains 1 PH domain. Contains 1 SEC7 domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Coiled coil
Molecular function	Guanine-nucleotide releasing factor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of cell adhesion Inferred from direct assay Ref.3. Source: MGI regulation of ARF protein signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: MGI plasma membrane Inferred from direct assay Ref.3. Source: MGI ruffle Inferred from physical interaction. Source: MGI
Molecular function	ARF guanyl-nucleotide exchange factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome phosphatidylinositol-3,4,5-trisphosphate binding Inferred from direct assay Ref.1. Source: MGI protein binding Inferred from physical interaction Ref.5. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 399

399

Cytohesin-3

PRO_0000120201

Regions

Domain

77 – 206

130

SEC7

Domain

264 – 380

117

Coiled coil

14 – 61

Potential

Secondary structure

399

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

O08967 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 00F7FEB9C3849157
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FASTA

399

46,280

        10         20         30         40         50         60 
MDEGGGGEGG SVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT EIDNLTSVEE 

        70         80         90        100        110        120 
SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED VAQFLYKGEG LNKTVIGDYL 

       130        140        150        160        170        180 
GERDDFNIKV LQAFVELHEF ADLNLVQALR QFLWSFRLPG EAQKIDRMME AFASRYCLCN 

       190        200        210        220        230        240 
PGVFQSTDTC YVLSFAIIML NTSLHNHNVR DKPTAERFIT MNRGINEGGD LPEELLRNLY 

       250        260        270        280        290        300 
ESIKNEPFKI PEDDGNDLTH TFFNPDREGW LLKLGGRVKT WKRRWFILTD NCLYYFEYTT 

       310        320        330        340        350        360 
DKEPRGIIPL ENLSIREVED PRKPNCFELY NPSHKGQVIK ACKTEADGRV VEGNHVVYRI 

       370        380        390 
SAPSPEEKEE WMKSIKASIS RDPFYDMLAT RKRRIANKK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Signaling by phosphoinositide-3,4,5-trisphosphate through proteins containing pleckstrin and Sec7 homology domains." Klarlund J.K., Guilherme A., Holik J.J., Virbasius J.V., Chawla A., Czech M.P. Science 275:1927-1930(1997) [PubMed: 9072969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complex regulation of multiple cytohesin-like genes in murine tissues and cells." Kim H.-S., Chen Y., Lonai P. FEBS Lett. 433:312-316(1998) [PubMed: 9744817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Anergic T lymphocytes selectively express an integrin regulatory protein of the cytohesin family." Korthauer U., Nagel W., Davis E.M., Le Beau M.M., Menon R.S., Mitchell E.O., Kozak C.A., Kolanus W., Bluestone J.A. J. Immunol. 164:308-318(2000) [PubMed: 10605025] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: NIH Swiss.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[5]	"Interaction of GRASP, a protein encoded by a novel retinoic acid-induced gene, with members of the cytohesin family of guanine nucleotide exchange factors." Nevrivy D.J., Peterson V.J., Avram D., Ishmael J.E., Hansen S.G., Dowell P., Hruby D.E., Dawson M.I., Leid M. J. Biol. Chem. 275:16827-16836(2000) [PubMed: 10828067] [Abstract] Cited for: INTERACTION WITH GRASP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF001871 Genomic DNA. Translation: AAB60876.1.
AB013470 mRNA. Translation: BAA33433.1.
AF084221 mRNA. Translation: AAF23858.1.
BC035296 mRNA. Translation: AAH35296.2.

IPI

IPI00272148.

RefSeq

NP_035312.3. NM_011182.4.

UniGene

Mm.281003.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FGY	X-ray	1.50	A	261-387	[»]
1FGZ	X-ray	2.05	A	261-387	[»]
1FHW	X-ray	1.90	A/B	264-391	[»]
1FHX	X-ray	2.50	A/B	264-391	[»]
1U2B	X-ray	1.80	A	261-387	[»]
2R09	X-ray	1.90	A/B	63-399	[»]
2R0D	X-ray	2.04	A/B	63-399	[»]

ProteinModelPortal

O08967.

SMR

O08967. Positions 63-396.

ModBase

Search...

Protein-protein interaction databases

STRING

O08967.

PTM databases

PhosphoSite

O08967.

Proteomic databases

PRIDE

O08967.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000116456; ENSMUSP00000112157; ENSMUSG00000018001.

GeneID

19159.

KEGG

mmu:19159.

Organism-specific databases

CTD

9265.

MGI

MGI:1335107. Cyth3.

Phylogenomic databases

eggNOG

roNOG08244.

HOVERGEN

HBG002647.

InParanoid

O08967.

OMA

WMKSIRA.

OrthoDB

EOG4J1187.

PhylomeDB

O08967.

Gene expression databases

ArrayExpress

O08967.

Bgee

O08967.

Genevestigator

O08967.

GermOnline

ENSMUSG00000018001. Mus musculus.

Family and domain databases

InterPro

IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR000904. Sec7.
IPR023394. SEC7_alpha_orthog.
[Graphical view]

Gene3D

G3DSA:1.10.1000.11. G3DSA:1.10.1000.11. 1 hit.
G3DSA:2.30.29.30. PH_type. 1 hit.

Pfam

PF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]

SMART

SM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]

SUPFAM

SSF48425. Sec7. 1 hit.

PROSITE

PS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

SOURCE

Search...

Entry information

Entry name

CYH3_MOUSE

Accession

Primary (citable) accession number: O08967
Secondary accession number(s): Q8CI93

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	July 1, 1997
Last modified:	November 16, 2011
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents