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O08966

- S22A1_MOUSE

UniProt

O08966 - S22A1_MOUSE

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Protein

Solute carrier family 22 member 1

Gene

Slc22a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei451 – 4511Involved in affinity and selectivity of cations as well as in translocationBy similarity

GO - Molecular functioni

  1. acetylcholine transmembrane transporter activity Source: Ensembl
  2. dopamine transmembrane transporter activity Source: Ensembl
  3. norepinephrine transmembrane transporter activity Source: Ensembl
  4. organic cation transmembrane transporter activity Source: MGI
  5. quaternary ammonium group transmembrane transporter activity Source: Ensembl
  6. secondary active organic cation transmembrane transporter activity Source: Ensembl

GO - Biological processi

  1. cation transport Source: MGI
  2. dopamine transport Source: Ensembl
  3. drug transmembrane transport Source: Ensembl
  4. epinephrine transport Source: Ensembl
  5. establishment or maintenance of transmembrane electrochemical gradient Source: Ensembl
  6. norepinephrine transport Source: Ensembl
  7. protein homooligomerization Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiREACT_196555. Abacavir transmembrane transport.
REACT_198610. Organic cation transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 22 member 1
Alternative name(s):
Organic cation transporter 1
Short name:
mOCT1
Gene namesi
Name:Slc22a1
Synonyms:Lx1, Oct1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:108111. Slc22a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei22 – 4221HelicalSequence AnalysisAdd
BLAST
Topological domaini43 – 150108ExtracellularSequence AnalysisAdd
BLAST
Transmembranei151 – 17121HelicalSequence AnalysisAdd
BLAST
Topological domaini172 – 1776CytoplasmicSequence Analysis
Transmembranei178 – 19821HelicalSequence AnalysisAdd
BLAST
Topological domaini199 – 21113ExtracellularSequence AnalysisAdd
BLAST
Transmembranei212 – 23120HelicalSequence AnalysisAdd
BLAST
Topological domaini232 – 2387CytoplasmicSequence Analysis
Transmembranei239 – 25921HelicalSequence AnalysisAdd
BLAST
Topological domaini260 – 2634ExtracellularSequence Analysis
Transmembranei264 – 28421HelicalSequence AnalysisAdd
BLAST
Topological domaini285 – 34864CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei349 – 36921HelicalSequence AnalysisAdd
BLAST
Topological domaini370 – 3778ExtracellularSequence Analysis
Transmembranei378 – 39821HelicalSequence AnalysisAdd
BLAST
Topological domaini399 – 4035CytoplasmicSequence Analysis
Transmembranei404 – 42421HelicalSequence AnalysisAdd
BLAST
Topological domaini425 – 4295ExtracellularSequence Analysis
Transmembranei430 – 45223HelicalSequence AnalysisAdd
BLAST
Topological domaini453 – 46513CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei466 – 48621HelicalSequence AnalysisAdd
BLAST
Topological domaini487 – 4937ExtracellularSequence Analysis
Transmembranei494 – 51421HelicalSequence AnalysisAdd
BLAST
Topological domaini515 – 55642CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: Ensembl
  2. integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Solute carrier family 22 member 1PRO_0000333876Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO08966.
PaxDbiO08966.
PRIDEiO08966.

Expressioni

Tissue specificityi

Highly expressed in liver, kidney and intestine. Weakly expressed in adrenals and in lacting mammary glands.2 Publications

Developmental stagei

Weakly expressed 2 days before birth, but gradually increased during the first 3 weeks of age, reaching a plateau around day 22 in both kidney and liver. At 45 days of age, renal and hepatic levels is 4 to 6 times higher than the level immediately after birth.1 Publication

Inductioni

Increased by PPARA and PPARG treatment in both liver and H35 cells.1 Publication

Gene expression databases

BgeeiO08966.
GenevestigatoriO08966.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024596.

Structurei

3D structure databases

ProteinModelPortaliO08966.
SMRiO08966. Positions 154-519.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00760000118852.
HOGENOMiHOG000234568.
HOVERGENiHBG061545.
InParanoidiO08966.
KOiK08198.
OMAiVCADSWK.
OrthoDBiEOG78PV8N.
PhylomeDBiO08966.
TreeFamiTF315847.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004749. Orgcat_transp.
IPR005828. Sub_transporter.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08966-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPTVDDVLEH VGEFGWFQKQ AFLLLCLISA SLAPIYVGIV FLGFTPDHHC
60 70 80 90 100
RSPGVAELSQ RCGWSPAEEL NYTVPGLGSA GEASFLSQCM KYEVDWNQST
110 120 130 140 150
LDCVDPLSSL AANRSHLPLS PCEHGWVYDT PGSSIVTEFN LVCGDAWKVD
160 170 180 190 200
LFQSCVNLGF FLGSLVVGYI ADRFGRKLCL LVTTLVTSLS GVLTAVAPDY
210 220 230 240 250
TSMLLFRLLQ GMVSKGSWVS GYTLITEFVG SGYRRTTAIL YQVAFTVGLV
260 270 280 290 300
GLAGVAYAIP DWRWLQLAVS LPTFLFLLYY WFVPESPRWL LSQKRTTQAV
310 320 330 340 350
RIMEQIAQKN RKVPPADLKM MCLEEDASER RSPSFADLFR TPSLRKHTLI
360 370 380 390 400
LMYLWFSCAV LYQGLIMHVG ATGANLYLDF FYSSLVEFPA AFIILVTIDR
410 420 430 440 450
IGRIYPIAAS NLVAGAACLL MIFIPHELHW LNVTLACLGR MGATIVLQMV
460 470 480 490 500
CLVNAELYPT FIRNLGMMVC SALCDLGGIF TPFMVFRLME VWQALPLILF
510 520 530 540 550
GVLGLSAGAV TLLLPETKGV ALPETIEEAE NLGRRKSKAK ENTIYLQVQT

GKSPHT
Length:556
Mass (Da):61,521
Last modified:May 20, 2008 - v2
Checksum:i0230EF5A2B1E2723
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261C → Y in AAB19097. (PubMed:8854860)Curated
Sequence conflicti144 – 1441G → V in AAB19097. (PubMed:8854860)Curated
Sequence conflicti506 – 5061S → T in AAB19097. (PubMed:8854860)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38652 mRNA. Translation: AAB19097.1.
AF010259 mRNA. Translation: AAC98884.1.
AF481054 Genomic DNA. Translation: AAM22157.1.
BC021651 mRNA. Translation: AAH21651.1.
CCDSiCCDS28393.1.
RefSeqiNP_033228.2. NM_009202.5.
UniGeneiMm.594.

Genome annotation databases

EnsembliENSMUST00000024596; ENSMUSP00000024596; ENSMUSG00000023829.
GeneIDi20517.
KEGGimmu:20517.
UCSCiuc008akx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38652 mRNA. Translation: AAB19097.1 .
AF010259 mRNA. Translation: AAC98884.1 .
AF481054 Genomic DNA. Translation: AAM22157.1 .
BC021651 mRNA. Translation: AAH21651.1 .
CCDSi CCDS28393.1.
RefSeqi NP_033228.2. NM_009202.5.
UniGenei Mm.594.

3D structure databases

ProteinModelPortali O08966.
SMRi O08966. Positions 154-519.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000024596.

Chemistry

BindingDBi O08966.
ChEMBLi CHEMBL2073664.

Proteomic databases

MaxQBi O08966.
PaxDbi O08966.
PRIDEi O08966.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000024596 ; ENSMUSP00000024596 ; ENSMUSG00000023829 .
GeneIDi 20517.
KEGGi mmu:20517.
UCSCi uc008akx.1. mouse.

Organism-specific databases

CTDi 6580.
MGIi MGI:108111. Slc22a1.

Phylogenomic databases

eggNOGi COG0477.
GeneTreei ENSGT00760000118852.
HOGENOMi HOG000234568.
HOVERGENi HBG061545.
InParanoidi O08966.
KOi K08198.
OMAi VCADSWK.
OrthoDBi EOG78PV8N.
PhylomeDBi O08966.
TreeFami TF315847.

Enzyme and pathway databases

Reactomei REACT_196555. Abacavir transmembrane transport.
REACT_198610. Organic cation transport.

Miscellaneous databases

NextBioi 298731.
PROi O08966.
SOURCEi Search...

Gene expression databases

Bgeei O08966.
Genevestigatori O08966.

Family and domain databases

InterProi IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004749. Orgcat_transp.
IPR005828. Sub_transporter.
IPR005829. Sugar_transporter_CS.
[Graphical view ]
Pfami PF00083. Sugar_tr. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 1 hit.
TIGRFAMsi TIGR00898. 2A0119. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Lx1 gene maps to mouse chromosome 17 and codes for a protein that is homologous to glucose and polyspecific transmembrane transporters."
    Schweifer N., Barlow D.P.
    Mamm. Genome 7:735-740(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Liver.
  2. "Cloning and functional expression of a mouse liver organic cation transporter."
    Green R.M., Lo K., Sterritt C., Beier D.R.
    Hepatology 29:1556-1562(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: C57BL/6 X CBA.
  3. "Functional characterization of mouse cation transporter mOCT2 compared with mOCT1."
    Kakehi M., Koyabu N., Nakamura T., Uchiumi T., Kuwano M., Ohtani H., Sawada Y.
    Biochem. Biophys. Res. Commun. 296:644-650(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: ddY.
    Tissue: Kidney.
  4. "Genomic sequence analysis in the mouse T-complex region."
    Brathwaite M., Waeltz P., Qian Y., Dudekula D., Schlessinger D., Nagaraja R.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129S6/SvEvTac.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  6. "Transcriptional regulation of murine Slc22a1 (Oct1) by peroxisome proliferator agonist receptor-alpha and -gamma."
    Nie W., Sweetser S., Rinella M., Green R.M.
    Am. J. Physiol. 288:G207-G212(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Tissue distribution and ontogeny of organic cation transporters in mice."
    Alnouti Y., Petrick J.S., Klaassen C.D.
    Drug Metab. Dispos. 34:477-482(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiS22A1_MOUSE
AccessioniPrimary (citable) accession number: O08966
Secondary accession number(s): Q9R1Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: November 26, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3