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Protein

Solute carrier family 22 member 1

Gene

Slc22a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei451Involved in affinity and selectivity of cations as well as in translocationBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-2161517. Abacavir transmembrane transport.
R-MMU-549127. Organic cation transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 22 member 1
Alternative name(s):
Organic cation transporter 1
Short name:
mOCT1
Gene namesi
Name:Slc22a1
Synonyms:Lx1, Oct1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:108111. Slc22a1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21CytoplasmicSequence analysisAdd BLAST21
Transmembranei22 – 42HelicalSequence analysisAdd BLAST21
Topological domaini43 – 150ExtracellularSequence analysisAdd BLAST108
Transmembranei151 – 171HelicalSequence analysisAdd BLAST21
Topological domaini172 – 177CytoplasmicSequence analysis6
Transmembranei178 – 198HelicalSequence analysisAdd BLAST21
Topological domaini199 – 211ExtracellularSequence analysisAdd BLAST13
Transmembranei212 – 231HelicalSequence analysisAdd BLAST20
Topological domaini232 – 238CytoplasmicSequence analysis7
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Topological domaini260 – 263ExtracellularSequence analysis4
Transmembranei264 – 284HelicalSequence analysisAdd BLAST21
Topological domaini285 – 348CytoplasmicSequence analysisAdd BLAST64
Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
Topological domaini370 – 377ExtracellularSequence analysis8
Transmembranei378 – 398HelicalSequence analysisAdd BLAST21
Topological domaini399 – 403CytoplasmicSequence analysis5
Transmembranei404 – 424HelicalSequence analysisAdd BLAST21
Topological domaini425 – 429ExtracellularSequence analysis5
Transmembranei430 – 452HelicalSequence analysisAdd BLAST23
Topological domaini453 – 465CytoplasmicSequence analysisAdd BLAST13
Transmembranei466 – 486HelicalSequence analysisAdd BLAST21
Topological domaini487 – 493ExtracellularSequence analysis7
Transmembranei494 – 514HelicalSequence analysisAdd BLAST21
Topological domaini515 – 556CytoplasmicSequence analysisAdd BLAST42

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2073664.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003338761 – 556Solute carrier family 22 member 1Add BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi71N-linked (GlcNAc...)Sequence analysis1
Modified residuei334PhosphoserineCombined sources1
Modified residuei543PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO08966.
PaxDbiO08966.
PeptideAtlasiO08966.
PRIDEiO08966.

PTM databases

iPTMnetiO08966.
PhosphoSitePlusiO08966.

Expressioni

Tissue specificityi

Highly expressed in liver, kidney and intestine. Weakly expressed in adrenals and in lacting mammary glands.2 Publications

Developmental stagei

Weakly expressed 2 days before birth, but gradually increased during the first 3 weeks of age, reaching a plateau around day 22 in both kidney and liver. At 45 days of age, renal and hepatic levels is 4 to 6 times higher than the level immediately after birth.1 Publication

Inductioni

Increased by PPARA and PPARG treatment in both liver and H35 cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000023829.
GenevisibleiO08966. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024596.

Chemistry databases

BindingDBiO08966.

Structurei

3D structure databases

ProteinModelPortaliO08966.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IRIE. Eukaryota.
ENOG410XSRI. LUCA.
GeneTreeiENSGT00760000118852.
HOGENOMiHOG000234568.
HOVERGENiHBG061545.
InParanoidiO08966.
KOiK08198.
OMAiDLFQSCL.
OrthoDBiEOG091G05AC.
PhylomeDBiO08966.
TreeFamiTF315847.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR004749. Orgcat_transp/SVOP.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTVDDVLEH VGEFGWFQKQ AFLLLCLISA SLAPIYVGIV FLGFTPDHHC
60 70 80 90 100
RSPGVAELSQ RCGWSPAEEL NYTVPGLGSA GEASFLSQCM KYEVDWNQST
110 120 130 140 150
LDCVDPLSSL AANRSHLPLS PCEHGWVYDT PGSSIVTEFN LVCGDAWKVD
160 170 180 190 200
LFQSCVNLGF FLGSLVVGYI ADRFGRKLCL LVTTLVTSLS GVLTAVAPDY
210 220 230 240 250
TSMLLFRLLQ GMVSKGSWVS GYTLITEFVG SGYRRTTAIL YQVAFTVGLV
260 270 280 290 300
GLAGVAYAIP DWRWLQLAVS LPTFLFLLYY WFVPESPRWL LSQKRTTQAV
310 320 330 340 350
RIMEQIAQKN RKVPPADLKM MCLEEDASER RSPSFADLFR TPSLRKHTLI
360 370 380 390 400
LMYLWFSCAV LYQGLIMHVG ATGANLYLDF FYSSLVEFPA AFIILVTIDR
410 420 430 440 450
IGRIYPIAAS NLVAGAACLL MIFIPHELHW LNVTLACLGR MGATIVLQMV
460 470 480 490 500
CLVNAELYPT FIRNLGMMVC SALCDLGGIF TPFMVFRLME VWQALPLILF
510 520 530 540 550
GVLGLSAGAV TLLLPETKGV ALPETIEEAE NLGRRKSKAK ENTIYLQVQT

GKSPHT
Length:556
Mass (Da):61,521
Last modified:May 20, 2008 - v2
Checksum:i0230EF5A2B1E2723
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26C → Y in AAB19097 (PubMed:8854860).Curated1
Sequence conflicti144G → V in AAB19097 (PubMed:8854860).Curated1
Sequence conflicti506S → T in AAB19097 (PubMed:8854860).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38652 mRNA. Translation: AAB19097.1.
AF010259 mRNA. Translation: AAC98884.1.
AF481054 Genomic DNA. Translation: AAM22157.1.
BC021651 mRNA. Translation: AAH21651.1.
CCDSiCCDS28393.1.
RefSeqiNP_033228.2. NM_009202.5.
UniGeneiMm.594.

Genome annotation databases

EnsembliENSMUST00000024596; ENSMUSP00000024596; ENSMUSG00000023829.
GeneIDi20517.
KEGGimmu:20517.
UCSCiuc008akx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38652 mRNA. Translation: AAB19097.1.
AF010259 mRNA. Translation: AAC98884.1.
AF481054 Genomic DNA. Translation: AAM22157.1.
BC021651 mRNA. Translation: AAH21651.1.
CCDSiCCDS28393.1.
RefSeqiNP_033228.2. NM_009202.5.
UniGeneiMm.594.

3D structure databases

ProteinModelPortaliO08966.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024596.

Chemistry databases

BindingDBiO08966.
ChEMBLiCHEMBL2073664.

PTM databases

iPTMnetiO08966.
PhosphoSitePlusiO08966.

Proteomic databases

MaxQBiO08966.
PaxDbiO08966.
PeptideAtlasiO08966.
PRIDEiO08966.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024596; ENSMUSP00000024596; ENSMUSG00000023829.
GeneIDi20517.
KEGGimmu:20517.
UCSCiuc008akx.1. mouse.

Organism-specific databases

CTDi6580.
MGIiMGI:108111. Slc22a1.

Phylogenomic databases

eggNOGiENOG410IRIE. Eukaryota.
ENOG410XSRI. LUCA.
GeneTreeiENSGT00760000118852.
HOGENOMiHOG000234568.
HOVERGENiHBG061545.
InParanoidiO08966.
KOiK08198.
OMAiDLFQSCL.
OrthoDBiEOG091G05AC.
PhylomeDBiO08966.
TreeFamiTF315847.

Enzyme and pathway databases

ReactomeiR-MMU-2161517. Abacavir transmembrane transport.
R-MMU-549127. Organic cation transport.

Miscellaneous databases

PROiO08966.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023829.
GenevisibleiO08966. MM.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR004749. Orgcat_transp/SVOP.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS22A1_MOUSE
AccessioniPrimary (citable) accession number: O08966
Secondary accession number(s): Q9R1Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: November 30, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.