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O08966

- S22A1_MOUSE

UniProt

O08966 - S22A1_MOUSE

Protein

Solute carrier family 22 member 1

Gene

Slc22a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (20 May 2008)
      Previous versions | rss
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    Functioni

    Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei451 – 4511Involved in affinity and selectivity of cations as well as in translocationBy similarity

    GO - Molecular functioni

    1. acetylcholine transmembrane transporter activity Source: Ensembl
    2. dopamine transmembrane transporter activity Source: Ensembl
    3. norepinephrine transmembrane transporter activity Source: Ensembl
    4. organic cation transmembrane transporter activity Source: MGI
    5. quaternary ammonium group transmembrane transporter activity Source: Ensembl
    6. secondary active organic cation transmembrane transporter activity Source: Ensembl

    GO - Biological processi

    1. cation transport Source: MGI
    2. dopamine transport Source: Ensembl
    3. drug transmembrane transport Source: Ensembl
    4. epinephrine transport Source: Ensembl
    5. establishment or maintenance of transmembrane electrochemical gradient Source: Ensembl
    6. norepinephrine transport Source: Ensembl
    7. protein homooligomerization Source: Ensembl

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_196555. Abacavir transmembrane transport.
    REACT_198610. Organic cation transport.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Solute carrier family 22 member 1
    Alternative name(s):
    Organic cation transporter 1
    Short name:
    mOCT1
    Gene namesi
    Name:Slc22a1
    Synonyms:Lx1, Oct1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:108111. Slc22a1.

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB-SubCell
    2. integral component of plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 556556Solute carrier family 22 member 1PRO_0000333876Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Phosphorylated.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiO08966.
    PaxDbiO08966.
    PRIDEiO08966.

    Expressioni

    Tissue specificityi

    Highly expressed in liver, kidney and intestine. Weakly expressed in adrenals and in lacting mammary glands.2 Publications

    Developmental stagei

    Weakly expressed 2 days before birth, but gradually increased during the first 3 weeks of age, reaching a plateau around day 22 in both kidney and liver. At 45 days of age, renal and hepatic levels is 4 to 6 times higher than the level immediately after birth.1 Publication

    Inductioni

    Increased by PPARA and PPARG treatment in both liver and H35 cells.1 Publication

    Gene expression databases

    BgeeiO08966.
    GenevestigatoriO08966.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000024596.

    Structurei

    3D structure databases

    ProteinModelPortaliO08966.
    SMRiO08966. Positions 154-519.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2121CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini43 – 150108ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini172 – 1776CytoplasmicSequence Analysis
    Topological domaini199 – 21113ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini232 – 2387CytoplasmicSequence Analysis
    Topological domaini260 – 2634ExtracellularSequence Analysis
    Topological domaini285 – 34864CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini370 – 3778ExtracellularSequence Analysis
    Topological domaini399 – 4035CytoplasmicSequence Analysis
    Topological domaini425 – 4295ExtracellularSequence Analysis
    Topological domaini453 – 46513CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini487 – 4937ExtracellularSequence Analysis
    Topological domaini515 – 55642CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei22 – 4221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei151 – 17121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei178 – 19821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei212 – 23120HelicalSequence AnalysisAdd
    BLAST
    Transmembranei239 – 25921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei264 – 28421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei349 – 36921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei378 – 39821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei404 – 42421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei430 – 45223HelicalSequence AnalysisAdd
    BLAST
    Transmembranei466 – 48621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei494 – 51421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00730000110561.
    HOGENOMiHOG000234568.
    HOVERGENiHBG061545.
    InParanoidiO08966.
    KOiK08198.
    OMAiVCADSWK.
    OrthoDBiEOG78PV8N.
    PhylomeDBiO08966.
    TreeFamiTF315847.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004749. Orgcat_transp.
    IPR005828. Sub_transporter.
    IPR005829. Sugar_transporter_CS.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00898. 2A0119. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O08966-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPTVDDVLEH VGEFGWFQKQ AFLLLCLISA SLAPIYVGIV FLGFTPDHHC    50
    RSPGVAELSQ RCGWSPAEEL NYTVPGLGSA GEASFLSQCM KYEVDWNQST 100
    LDCVDPLSSL AANRSHLPLS PCEHGWVYDT PGSSIVTEFN LVCGDAWKVD 150
    LFQSCVNLGF FLGSLVVGYI ADRFGRKLCL LVTTLVTSLS GVLTAVAPDY 200
    TSMLLFRLLQ GMVSKGSWVS GYTLITEFVG SGYRRTTAIL YQVAFTVGLV 250
    GLAGVAYAIP DWRWLQLAVS LPTFLFLLYY WFVPESPRWL LSQKRTTQAV 300
    RIMEQIAQKN RKVPPADLKM MCLEEDASER RSPSFADLFR TPSLRKHTLI 350
    LMYLWFSCAV LYQGLIMHVG ATGANLYLDF FYSSLVEFPA AFIILVTIDR 400
    IGRIYPIAAS NLVAGAACLL MIFIPHELHW LNVTLACLGR MGATIVLQMV 450
    CLVNAELYPT FIRNLGMMVC SALCDLGGIF TPFMVFRLME VWQALPLILF 500
    GVLGLSAGAV TLLLPETKGV ALPETIEEAE NLGRRKSKAK ENTIYLQVQT 550
    GKSPHT 556
    Length:556
    Mass (Da):61,521
    Last modified:May 20, 2008 - v2
    Checksum:i0230EF5A2B1E2723
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261C → Y in AAB19097. (PubMed:8854860)Curated
    Sequence conflicti144 – 1441G → V in AAB19097. (PubMed:8854860)Curated
    Sequence conflicti506 – 5061S → T in AAB19097. (PubMed:8854860)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U38652 mRNA. Translation: AAB19097.1.
    AF010259 mRNA. Translation: AAC98884.1.
    AF481054 Genomic DNA. Translation: AAM22157.1.
    BC021651 mRNA. Translation: AAH21651.1.
    CCDSiCCDS28393.1.
    RefSeqiNP_033228.2. NM_009202.5.
    UniGeneiMm.594.

    Genome annotation databases

    EnsembliENSMUST00000024596; ENSMUSP00000024596; ENSMUSG00000023829.
    GeneIDi20517.
    KEGGimmu:20517.
    UCSCiuc008akx.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U38652 mRNA. Translation: AAB19097.1 .
    AF010259 mRNA. Translation: AAC98884.1 .
    AF481054 Genomic DNA. Translation: AAM22157.1 .
    BC021651 mRNA. Translation: AAH21651.1 .
    CCDSi CCDS28393.1.
    RefSeqi NP_033228.2. NM_009202.5.
    UniGenei Mm.594.

    3D structure databases

    ProteinModelPortali O08966.
    SMRi O08966. Positions 154-519.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000024596.

    Chemistry

    ChEMBLi CHEMBL2073664.

    Proteomic databases

    MaxQBi O08966.
    PaxDbi O08966.
    PRIDEi O08966.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024596 ; ENSMUSP00000024596 ; ENSMUSG00000023829 .
    GeneIDi 20517.
    KEGGi mmu:20517.
    UCSCi uc008akx.1. mouse.

    Organism-specific databases

    CTDi 6580.
    MGIi MGI:108111. Slc22a1.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00730000110561.
    HOGENOMi HOG000234568.
    HOVERGENi HBG061545.
    InParanoidi O08966.
    KOi K08198.
    OMAi VCADSWK.
    OrthoDBi EOG78PV8N.
    PhylomeDBi O08966.
    TreeFami TF315847.

    Enzyme and pathway databases

    Reactomei REACT_196555. Abacavir transmembrane transport.
    REACT_198610. Organic cation transport.

    Miscellaneous databases

    NextBioi 298731.
    PROi O08966.
    SOURCEi Search...

    Gene expression databases

    Bgeei O08966.
    Genevestigatori O08966.

    Family and domain databases

    InterProi IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004749. Orgcat_transp.
    IPR005828. Sub_transporter.
    IPR005829. Sugar_transporter_CS.
    [Graphical view ]
    Pfami PF00083. Sugar_tr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 1 hit.
    TIGRFAMsi TIGR00898. 2A0119. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Lx1 gene maps to mouse chromosome 17 and codes for a protein that is homologous to glucose and polyspecific transmembrane transporters."
      Schweifer N., Barlow D.P.
      Mamm. Genome 7:735-740(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: C57BL/6.
      Tissue: Liver.
    2. "Cloning and functional expression of a mouse liver organic cation transporter."
      Green R.M., Lo K., Sterritt C., Beier D.R.
      Hepatology 29:1556-1562(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Strain: C57BL/6 X CBA.
    3. "Functional characterization of mouse cation transporter mOCT2 compared with mOCT1."
      Kakehi M., Koyabu N., Nakamura T., Uchiumi T., Kuwano M., Ohtani H., Sawada Y.
      Biochem. Biophys. Res. Commun. 296:644-650(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Strain: ddY.
      Tissue: Kidney.
    4. "Genomic sequence analysis in the mouse T-complex region."
      Brathwaite M., Waeltz P., Qian Y., Dudekula D., Schlessinger D., Nagaraja R.
      Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 129S6/SvEvTac.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    6. "Transcriptional regulation of murine Slc22a1 (Oct1) by peroxisome proliferator agonist receptor-alpha and -gamma."
      Nie W., Sweetser S., Rinella M., Green R.M.
      Am. J. Physiol. 288:G207-G212(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "Tissue distribution and ontogeny of organic cation transporters in mice."
      Alnouti Y., Petrick J.S., Klaassen C.D.
      Drug Metab. Dispos. 34:477-482(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiS22A1_MOUSE
    AccessioniPrimary (citable) accession number: O08966
    Secondary accession number(s): Q9R1Q4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3