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O08962

- KCNH2_RAT

UniProt

O08962 - KCNH2_RAT

Protein

Potassium voltage-gated channel subfamily H member 2

Gene

Kcnh2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr) By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi744 – 861118cNMPAdd
    BLAST

    GO - Molecular functioni

    1. inward rectifier potassium channel activity Source: RGD
    2. ion channel activity Source: RGD
    3. phosphorelay sensor kinase activity Source: InterPro
    4. potassium channel activity Source: RGD
    5. protein binding Source: BHF-UCL
    6. voltage-gated potassium channel activity Source: RGD

    GO - Biological processi

    1. brain development Source: RGD
    2. potassium ion transmembrane transport Source: RefGenome
    3. potassium ion transport Source: RGD
    4. protein heterooligomerization Source: RGD
    5. regulation of heart rate by cardiac conduction Source: RefGenome
    6. regulation of membrane potential Source: RGD
    7. spinal cord development Source: RGD

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily H member 2
    Alternative name(s):
    Ether-a-go-go-related gene potassium channel 1
    Short name:
    ERG-1
    Short name:
    Eag-related protein 1
    Short name:
    Ether-a-go-go-related protein 1
    Short name:
    RERG
    Short name:
    r-ERG
    Voltage-gated potassium channel subunit Kv11.1
    Gene namesi
    Name:Kcnh2
    Synonyms:Erg
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621414. Kcnh2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: RGD

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi630 – 6301G → S: Dominant negative mutant; abolishes ERG current. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11631163Potassium voltage-gated channel subfamily H member 2PRO_0000054003Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei322 – 3221PhosphoserineBy similarity
    Glycosylationi600 – 6001N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Phosphorylated on serine and threonine residues.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiO08962.
    PRIDEiO08962.

    PTM databases

    PhosphoSiteiO08962.

    Expressioni

    Tissue specificityi

    Highly expressed in brain and testis, slightly less so in heart, adrenal, retina and thymus. Detected at lower levels in lung, soleus, tibialis, and at very low levels in cornea and lens. A shorter transcript is detected in skeletal muscle. Found in pituitary.1 Publication

    Gene expression databases

    GenevestigatoriO08962.

    Interactioni

    Subunit structurei

    The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH6/ERG2 and KCNH7/ERG3. Heteromultimer with KCNE1 and KCNE2 By similarity. Interacts with ALG10B. Interacts with CANX By similarity.By similarity

    Protein-protein interaction databases

    BioGridi250619. 1 interaction.
    STRINGi10116.ENSRNOP00000013800.

    Structurei

    3D structure databases

    ProteinModelPortaliO08962.
    SMRiO08962. Positions 26-135, 572-613.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 405405CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini427 – 45226ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini474 – 49724CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini519 – 5224ExtracellularSequence Analysis
    Topological domaini544 – 5496CytoplasmicSequence Analysis
    Topological domaini571 – 61343ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini635 – 6406ExtracellularSequence Analysis
    Topological domaini662 – 1163502CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei614 – 63421Pore-forming; Name=Segment H5Sequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei406 – 42621Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei453 – 47321Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei498 – 51821Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei523 – 54321Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei550 – 57021Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei641 – 66121Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini17 – 8872PASPROSITE-ProRule annotationAdd
    BLAST
    Domaini92 – 14453PACPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi626 – 6316Selectivity filterBy similarity

    Domaini

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
    Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2202.
    HOGENOMiHOG000230793.
    HOVERGENiHBG052232.
    InParanoidiO08962.
    KOiK04905.
    PhylomeDBiO08962.

    Family and domain databases

    Gene3Di2.60.120.10. 2 hits.
    InterProiIPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
    IPR003967. K_chnl_volt-dep_ERG.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR000700. PAS-assoc_C.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF13426. PAS_9. 1 hit.
    [Graphical view]
    PRINTSiPR01463. EAGCHANLFMLY.
    PR01470. ERGCHANNEL.
    SMARTiSM00100. cNMP. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    SSF55785. SSF55785. 1 hit.
    TIGRFAMsiTIGR00229. sensory_box. 1 hit.
    PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
    PS50113. PAC. 1 hit.
    PS50112. PAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O08962-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPVRRGHVAP QNTFLDTIIR KFEGQSRKFI IANARVENCA VIYCNDGFCE     50
    LCGYSRAEVM QRPCTCDFLH GPRTQRRAAA QIAQALLGAE ERKVEIAFYR 100
    KDGSCFLCLV DVVPVKNEDG AVIMFILNFE VVMEKDMVGS PAHDTNHRGP 150
    STSWLASGRA KTFRLKLPAL LALTARESPM RTGSTGSPGA PGAVVVDVDL 200
    TPAAPSSESL ALDEVSAMDN HVAGLGPAEE RRALVGPASA SPVASIPGPH 250
    PSPRAQSLNP DASGSSCSLA RTRSRESCAS VRRASSADDI EAMRAGALPL 300
    PPRHASTGAM HPLRSGLLNS TSDSDLVRYR TISKIPQITL NFVDLKGDPF 350
    LASPTSDREI IAPKIKERTH NVTEKVTQVL SLGADVLPEY KLQAPRIHRW 400
    TILHYSPFKA VWDWLILLLV IYTAVFTPYS AAFLLKETED GSQAPDCGYA 450
    CQPLAVVDLL VDIMFIVDIL INFRTTYVNA NEEVVSHPGR IAVHYFKGWF 500
    LIDMVAAIPF DLLIFGSGSE ELIGLLKTAR LLRLVRVARK LDRYSEYGAA 550
    VLFLLMCTFA LIAHWLACIW YAIGNMEQPH MDSHIGWLHN LGDQIGKPYN 600
    SSGLGGPSIK DKYVTALYFT FSSLTSVGFG NVSPNTNSEK IFSICVMLIG 650
    SLMYASIFGN VSAIIQRLYS GTARYHTQML RVREFIRFHQ IPNPLRQRLE 700
    EYFQHAWSYT NGIDMNAVLK GFPECLQADI CLHLNRSLLQ HCKPFRGATK 750
    GCLRALAMKF KTTHAPPGDT LVHAGDLLTA LYFISRGSIE ILRGDVVVAI 800
    LGKNDIFGEP LNLYARPGKS NGDVRALTYC DLHKIHRDDL LEVLDMYPEF 850
    SDHFWSSLEI TFNLRDTNMI PGSPSSAELE SGFNRQRKRK LSFRRRTDKD 900
    TEQPGEVSAL GQGPARVGPG PSCRGQPGGP WGESPSSGPS SPESSEDEGP 950
    GRSSSPLRLV PFSSPRPPGD SPGGEPLTED GEKSSDTCNP LSGAFSGVSN 1000
    IFSFWGDSRG RQYQELPRCP APAPSLLNIP LSSPGRRSRG DVESRLDALQ 1050
    RQLNRLETRL SADMATVLQL LQRQMTLVPP AYSAVTTPGP GPTSTSPLLP 1100
    VGPVPTLTLD SLSQVSQFVA FEELPAGAPE LPQDGPTRRL SLPGQLGALT 1150
    SQPLHRHGSD PGS 1163
    Length:1,163
    Mass (Da):126,952
    Last modified:July 1, 1997 - v1
    Checksum:iF0D75B0B532D9EA2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti411 – 4111V → A in AAC53160. (PubMed:9012748)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z96106 mRNA. Translation: CAB09536.1.
    U75210 mRNA. Translation: AAC53160.1.
    RefSeqiNP_446401.1. NM_053949.1.
    UniGeneiRn.10970.

    Genome annotation databases

    GeneIDi117018.
    KEGGirno:117018.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z96106 mRNA. Translation: CAB09536.1 .
    U75210 mRNA. Translation: AAC53160.1 .
    RefSeqi NP_446401.1. NM_053949.1.
    UniGenei Rn.10970.

    3D structure databases

    ProteinModelPortali O08962.
    SMRi O08962. Positions 26-135, 572-613.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250619. 1 interaction.
    STRINGi 10116.ENSRNOP00000013800.

    Chemistry

    GuidetoPHARMACOLOGYi 572.

    PTM databases

    PhosphoSitei O08962.

    Proteomic databases

    PaxDbi O08962.
    PRIDEi O08962.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 117018.
    KEGGi rno:117018.

    Organism-specific databases

    CTDi 3757.
    RGDi 621414. Kcnh2.

    Phylogenomic databases

    eggNOGi COG2202.
    HOGENOMi HOG000230793.
    HOVERGENi HBG052232.
    InParanoidi O08962.
    KOi K04905.
    PhylomeDBi O08962.

    Miscellaneous databases

    NextBioi 619763.
    PROi O08962.

    Gene expression databases

    Genevestigatori O08962.

    Family and domain databases

    Gene3Di 2.60.120.10. 2 hits.
    InterProi IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
    IPR003967. K_chnl_volt-dep_ERG.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR000700. PAS-assoc_C.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF13426. PAS_9. 1 hit.
    [Graphical view ]
    PRINTSi PR01463. EAGCHANLFMLY.
    PR01470. ERGCHANNEL.
    SMARTi SM00100. cNMP. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    SSF55785. SSF55785. 1 hit.
    TIGRFAMsi TIGR00229. sensory_box. 1 hit.
    PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
    PS50113. PAC. 1 hit.
    PS50112. PAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "RERG is a molecular correlate of the inward-rectifying K current in clonal rat pituitary cells."
      Bauer C.K., Engeland B., Wulfsen I., Ludwig J., Pongs O., Schwarz J.R.
      Recept. Channels 6:19-29(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain cortex.
    2. "Tissue and species distribution of mRNA for the IKr-like K+ channel, erg."
      Wymore R.S., Gintant G.A., Wymore R.T., Dixon J.E., McKinnon D., Cohen I.S.
      Circ. Res. 80:261-268(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 409-568.
    3. "Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary."
      Wulfsen I., Hauber H.-P., Schiemann D., Bauer C.K., Schwarz J.R.
      J. Neuroendocrinol. 12:263-272(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. "Erg1, erg2 and erg3 K channel subunits are able to form heteromultimers."
      Wimmers S., Wulfsen I., Bauer C.K., Schwarz J.R.
      Pflugers Arch. 441:450-455(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KCNH6 AND KCNH7, MUTAGENESIS OF GLY-630.
    5. "The IKr drug response is modulated by KCR1 in transfected cardiac and noncardiac cell lines."
      Kupershmidt S., Yang I.C.-H., Hayashi K., Wei J., Chanthaphaychith S., Petersen C.I., Johns D.C., George A.L. Jr., Roden D.M., Balser J.R.
      FASEB J. 17:2263-2265(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ALG10B.

    Entry informationi

    Entry nameiKCNH2_RAT
    AccessioniPrimary (citable) accession number: O08962
    Secondary accession number(s): O08720
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2002
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3