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Protein

Potassium voltage-gated channel subfamily H member 2

Gene

Kcnh2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr) (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi744 – 861118cNMPAdd
BLAST

GO - Molecular functioni

  • inward rectifier potassium channel activity Source: RGD
  • ion channel activity Source: RGD
  • phosphorelay sensor kinase activity Source: InterPro
  • potassium channel activity Source: RGD
  • scaffold protein binding Source: BHF-UCL
  • voltage-gated potassium channel activity Source: RGD

GO - Biological processi

  • brain development Source: RGD
  • potassium ion transmembrane transport Source: GO_Central
  • potassium ion transport Source: RGD
  • protein heterooligomerization Source: RGD
  • regulation of heart rate by cardiac conduction Source: GO_Central
  • regulation of membrane potential Source: RGD
  • spinal cord development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily H member 2
Alternative name(s):
Ether-a-go-go-related gene potassium channel 1
Short name:
ERG-1
Short name:
Eag-related protein 1
Short name:
Ether-a-go-go-related protein 1
Short name:
RERG
Short name:
r-ERG
Voltage-gated potassium channel subunit Kv11.1
Gene namesi
Name:Kcnh2
Synonyms:Erg
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621414. Kcnh2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 405405CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei406 – 42621Helical; Name=Segment S1Sequence AnalysisAdd
BLAST
Topological domaini427 – 45226ExtracellularSequence AnalysisAdd
BLAST
Transmembranei453 – 47321Helical; Name=Segment S2Sequence AnalysisAdd
BLAST
Topological domaini474 – 49724CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei498 – 51821Helical; Name=Segment S3Sequence AnalysisAdd
BLAST
Topological domaini519 – 5224ExtracellularSequence Analysis
Transmembranei523 – 54321Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
BLAST
Topological domaini544 – 5496CytoplasmicSequence Analysis
Transmembranei550 – 57021Helical; Name=Segment S5Sequence AnalysisAdd
BLAST
Topological domaini571 – 61343ExtracellularSequence AnalysisAdd
BLAST
Intramembranei614 – 63421Pore-forming; Name=Segment H5Sequence AnalysisAdd
BLAST
Topological domaini635 – 6406ExtracellularSequence Analysis
Transmembranei641 – 66121Helical; Name=Segment S6Sequence AnalysisAdd
BLAST
Topological domaini662 – 1163502CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi630 – 6301G → S: Dominant negative mutant; abolishes ERG current. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11631163Potassium voltage-gated channel subfamily H member 2PRO_0000054003Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei322 – 3221PhosphoserineBy similarity
Glycosylationi600 – 6001N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Phosphorylated on serine and threonine residues.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO08962.
PRIDEiO08962.

PTM databases

PhosphoSiteiO08962.

Expressioni

Tissue specificityi

Highly expressed in brain and testis, slightly less so in heart, adrenal, retina and thymus. Detected at lower levels in lung, soleus, tibialis, and at very low levels in cornea and lens. A shorter transcript is detected in skeletal muscle. Found in pituitary.1 Publication

Gene expression databases

GenevestigatoriO08962.

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH6/ERG2 and KCNH7/ERG3. Heteromultimer with KCNE1 and KCNE2 (By similarity). Interacts with ALG10B. Interacts with CANX (By similarity).By similarity

Protein-protein interaction databases

BioGridi250619. 1 interaction.
IntActiO08962. 1 interaction.
STRINGi10116.ENSRNOP00000013800.

Structurei

3D structure databases

ProteinModelPortaliO08962.
SMRiO08962. Positions 26-135, 572-613.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 8872PASPROSITE-ProRule annotationAdd
BLAST
Domaini92 – 14453PACPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi626 – 6316Selectivity filterBy similarity

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2202.
HOGENOMiHOG000230793.
HOVERGENiHBG052232.
InParanoidiO08962.
KOiK04905.
PhylomeDBiO08962.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR003967. K_chnl_volt-dep_ERG.
IPR030172. KCNH2.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF468. PTHR10217:SF468. 1 hit.
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
PR01470. ERGCHANNEL.
SMARTiSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVRRGHVAP QNTFLDTIIR KFEGQSRKFI IANARVENCA VIYCNDGFCE
60 70 80 90 100
LCGYSRAEVM QRPCTCDFLH GPRTQRRAAA QIAQALLGAE ERKVEIAFYR
110 120 130 140 150
KDGSCFLCLV DVVPVKNEDG AVIMFILNFE VVMEKDMVGS PAHDTNHRGP
160 170 180 190 200
STSWLASGRA KTFRLKLPAL LALTARESPM RTGSTGSPGA PGAVVVDVDL
210 220 230 240 250
TPAAPSSESL ALDEVSAMDN HVAGLGPAEE RRALVGPASA SPVASIPGPH
260 270 280 290 300
PSPRAQSLNP DASGSSCSLA RTRSRESCAS VRRASSADDI EAMRAGALPL
310 320 330 340 350
PPRHASTGAM HPLRSGLLNS TSDSDLVRYR TISKIPQITL NFVDLKGDPF
360 370 380 390 400
LASPTSDREI IAPKIKERTH NVTEKVTQVL SLGADVLPEY KLQAPRIHRW
410 420 430 440 450
TILHYSPFKA VWDWLILLLV IYTAVFTPYS AAFLLKETED GSQAPDCGYA
460 470 480 490 500
CQPLAVVDLL VDIMFIVDIL INFRTTYVNA NEEVVSHPGR IAVHYFKGWF
510 520 530 540 550
LIDMVAAIPF DLLIFGSGSE ELIGLLKTAR LLRLVRVARK LDRYSEYGAA
560 570 580 590 600
VLFLLMCTFA LIAHWLACIW YAIGNMEQPH MDSHIGWLHN LGDQIGKPYN
610 620 630 640 650
SSGLGGPSIK DKYVTALYFT FSSLTSVGFG NVSPNTNSEK IFSICVMLIG
660 670 680 690 700
SLMYASIFGN VSAIIQRLYS GTARYHTQML RVREFIRFHQ IPNPLRQRLE
710 720 730 740 750
EYFQHAWSYT NGIDMNAVLK GFPECLQADI CLHLNRSLLQ HCKPFRGATK
760 770 780 790 800
GCLRALAMKF KTTHAPPGDT LVHAGDLLTA LYFISRGSIE ILRGDVVVAI
810 820 830 840 850
LGKNDIFGEP LNLYARPGKS NGDVRALTYC DLHKIHRDDL LEVLDMYPEF
860 870 880 890 900
SDHFWSSLEI TFNLRDTNMI PGSPSSAELE SGFNRQRKRK LSFRRRTDKD
910 920 930 940 950
TEQPGEVSAL GQGPARVGPG PSCRGQPGGP WGESPSSGPS SPESSEDEGP
960 970 980 990 1000
GRSSSPLRLV PFSSPRPPGD SPGGEPLTED GEKSSDTCNP LSGAFSGVSN
1010 1020 1030 1040 1050
IFSFWGDSRG RQYQELPRCP APAPSLLNIP LSSPGRRSRG DVESRLDALQ
1060 1070 1080 1090 1100
RQLNRLETRL SADMATVLQL LQRQMTLVPP AYSAVTTPGP GPTSTSPLLP
1110 1120 1130 1140 1150
VGPVPTLTLD SLSQVSQFVA FEELPAGAPE LPQDGPTRRL SLPGQLGALT
1160
SQPLHRHGSD PGS
Length:1,163
Mass (Da):126,952
Last modified:July 1, 1997 - v1
Checksum:iF0D75B0B532D9EA2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti411 – 4111V → A in AAC53160 (PubMed:9012748).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z96106 mRNA. Translation: CAB09536.1.
U75210 mRNA. Translation: AAC53160.1.
RefSeqiNP_446401.1. NM_053949.1.
UniGeneiRn.10970.

Genome annotation databases

GeneIDi117018.
KEGGirno:117018.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z96106 mRNA. Translation: CAB09536.1.
U75210 mRNA. Translation: AAC53160.1.
RefSeqiNP_446401.1. NM_053949.1.
UniGeneiRn.10970.

3D structure databases

ProteinModelPortaliO08962.
SMRiO08962. Positions 26-135, 572-613.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250619. 1 interaction.
IntActiO08962. 1 interaction.
STRINGi10116.ENSRNOP00000013800.

Chemistry

GuidetoPHARMACOLOGYi572.

PTM databases

PhosphoSiteiO08962.

Proteomic databases

PaxDbiO08962.
PRIDEiO08962.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi117018.
KEGGirno:117018.

Organism-specific databases

CTDi3757.
RGDi621414. Kcnh2.

Phylogenomic databases

eggNOGiCOG2202.
HOGENOMiHOG000230793.
HOVERGENiHBG052232.
InParanoidiO08962.
KOiK04905.
PhylomeDBiO08962.

Miscellaneous databases

NextBioi619763.
PROiO08962.

Gene expression databases

GenevestigatoriO08962.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR003967. K_chnl_volt-dep_ERG.
IPR030172. KCNH2.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF468. PTHR10217:SF468. 1 hit.
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
PR01470. ERGCHANNEL.
SMARTiSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "RERG is a molecular correlate of the inward-rectifying K current in clonal rat pituitary cells."
    Bauer C.K., Engeland B., Wulfsen I., Ludwig J., Pongs O., Schwarz J.R.
    Recept. Channels 6:19-29(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain cortex.
  2. "Tissue and species distribution of mRNA for the IKr-like K+ channel, erg."
    Wymore R.S., Gintant G.A., Wymore R.T., Dixon J.E., McKinnon D., Cohen I.S.
    Circ. Res. 80:261-268(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 409-568.
  3. "Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary."
    Wulfsen I., Hauber H.-P., Schiemann D., Bauer C.K., Schwarz J.R.
    J. Neuroendocrinol. 12:263-272(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Erg1, erg2 and erg3 K channel subunits are able to form heteromultimers."
    Wimmers S., Wulfsen I., Bauer C.K., Schwarz J.R.
    Pflugers Arch. 441:450-455(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KCNH6 AND KCNH7, MUTAGENESIS OF GLY-630.
  5. "The IKr drug response is modulated by KCR1 in transfected cardiac and noncardiac cell lines."
    Kupershmidt S., Yang I.C.-H., Hayashi K., Wei J., Chanthaphaychith S., Petersen C.I., Johns D.C., George A.L. Jr., Roden D.M., Balser J.R.
    FASEB J. 17:2263-2265(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ALG10B.

Entry informationi

Entry nameiKCNH2_RAT
AccessioniPrimary (citable) accession number: O08962
Secondary accession number(s): O08720
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: July 1, 1997
Last modified: March 4, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.