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O08961 (ZN423_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein 423
Alternative name(s):
Olf1/EBF-associated zinc finger protein
Short name=rOAZ
Smad- and Olf-interacting zinc finger protein
Gene names
Name:Znf423
Synonyms:Oaz, Zfp423
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1311 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation. Ref.2

Subunit structure

Homodimer. Interacts with PARP1, SMAD1 and SMAD4 By similarity. Interacts with EBF1. Interacts with CEP290 By similarity. Ref.2 Ref.3

Subcellular location

Nucleus By similarity.

Tissue specificity

Expressed in brain, eye, olfactory epithelium, spleen and heart. Expressed in the basal layer, consisting of neural precursor cells and immature sensory neurons of the olfactory epithelium, but not in the mature receptor cells. Ref.2

Domain

Uses different DNA- and protein-binding zinc fingers to regulate the distinct BMP-Smad and Olf signaling pathways. C2H2-type zinc fingers 14-19 mediate the interaction with SMAD1 and SMAD4, while zinc fingers 28-30 mediate the interaction with EBF1. zinc fingers 2-8 bind the 5'-CCGCCC-3' DNA sequence in concert with EBF1, while zinc fingers 9-13 bind BMP target gene promoters in concert with SMADs By similarity. Ref.2 Ref.3

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 30 C2H2-type zinc fingers.

Sequence caution

The sequence AAA42353.1 differs from that shown. Reason: Frameshift at several positions.

The sequence AAB58646.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13111311Zinc finger protein 423
PRO_0000308597

Regions

Zinc finger75 – 10127C2H2-type 1; degenerate
Zinc finger146 – 16823C2H2-type 2
Zinc finger174 – 19623C2H2-type 3
Zinc finger202 – 22423C2H2-type 4
Zinc finger230 – 25223C2H2-type 5
Zinc finger271 – 29424C2H2-type 6
Zinc finger303 – 32624C2H2-type 7
Zinc finger331 – 35323C2H2-type 8
Zinc finger436 – 46025C2H2-type 9; degenerate
Zinc finger468 – 49124C2H2-type 10
Zinc finger507 – 53024C2H2-type 11
Zinc finger544 – 56724C2H2-type 12
Zinc finger590 – 61526C2H2-type 13; atypical
Zinc finger659 – 68123C2H2-type 14
Zinc finger689 – 71123C2H2-type 15
Zinc finger719 – 74224C2H2-type 16
Zinc finger747 – 77024C2H2-type 17
Zinc finger777 – 80024C2H2-type 18
Zinc finger808 – 83023C2H2-type 19
Zinc finger834 – 85724C2H2-type 20
Zinc finger913 – 93523C2H2-type 21; degenerate
Zinc finger957 – 97923C2H2-type 22
Zinc finger986 – 100823C2H2-type 23
Zinc finger1047 – 106923C2H2-type 24
Zinc finger1091 – 110919C2H2-type 25; degenerate
Zinc finger1147 – 117024C2H2-type 26
Zinc finger1195 – 121723C2H2-type 27
Zinc finger1225 – 124723C2H2-type 28
Zinc finger1256 – 127924C2H2-type 29
Zinc finger1286 – 130924C2H2-type 30

Amino acid modifications

Modified residue551Phosphoserine By similarity
Modified residue581Phosphoserine By similarity
Modified residue6311Phosphoserine By similarity
Modified residue10811Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
O08961 [UniParc].

Last modified October 23, 2007. Version 2.
Checksum: 6A5680DA502B7E36

FASTA1,311147,227
        10         20         30         40         50         60 
MSRRKQAKPR SVKVEEGEAS DFSLAWDSSV AAAGGLEGES ECDRKSSRAL EDRNSVTSQE 

        70         80         90        100        110        120 
ERNEDDEDVE DESIYTCDHC QQDFESLADL TDHRAHRCPG DGDDDPQLSW VASSPSSKDV 

       130        140        150        160        170        180 
ASPTQMIGDG CDLGLGEEEG GTGLPYPCQF CDKSFIRLSY LKRHEQIHSD KLPFKCTFCS 

       190        200        210        220        230        240 
RLFKHKRSRD RHIKLHTGDK KYHCHECEAA FSRRDHLKIH LKTHSSSKPF KCSVCKRGFS 

       250        260        270        280        290        300 
STSSLQSHMQ AHKKNKEHLA KSEKEAKKDD FMCDYCEDTF SQTEELEKHV LTLHPQLSEK 

       310        320        330        340        350        360 
ADLQCIHCPE VFVDESTLLA HIHQAHANQK HKCPMCPEQF SSVEGVYCHL DSHRQPDSSN 

       370        380        390        400        410        420 
HSVSPDPVLG SVASMSSATP DSTPDPVLGS VASMSSATPD SSASVERGST PDSTLKPLRG 

       430        440        450        460        470        480 
QKKMRDDGQS WSKVVYSCPY CSKRDFTSLA VLEIHLKTIH ADKPQQSHTC QICLDSMPTL 

       490        500        510        520        530        540 
YNLNEHVRKL HKSHAYPVMQ FGNISAFHCN YCPEMFADIN SLQEHIRVSH CGPNANPPDG 

       550        560        570        580        590        600 
NNAFFCNQCS MGFLTESSLT EHIQQAHCSV GSTKLESPVI QPTQSFMEVY SCPYCTNSPI 

       610        620        630        640        650        660 
FGSILKLTKH IKENHKNIPL AHSKKSKAEQ SPVSSDVEVS SPKRQRLSGS ANSISNGEYP 

       670        680        690        700        710        720 
CNQCDLKFSN FESFQTHLKL HLELLLRKQA CPQCKEDFDS QESLLQHLTV HYMTTSTHYV 

       730        740        750        760        770        780 
CESCDKQFSS VDDLQKHLLD MHTFVLYHCT LCQEVFDSKV SIQVHLAVKH SNEKKMYRCT 

       790        800        810        820        830        840 
ACNWDFRKEA DLQVHVKHSH LGNPAKAHKC IFCGETFSTE VELQCHITTH SKKYNCRFCS 

       850        860        870        880        890        900 
KAFHAVLLLE KHLREKHCVF DPAAENGTAN GVPPTSTKKA EPADLQGMLL KNPEAPNSHE 

       910        920        930        940        950        960 
ASEDDVDASE PMYGCDICGA AYTMEVLLQN HRLRDHNIRP GEDDGSRKKA EFIKGSHKCN 

       970        980        990       1000       1010       1020 
VCSRTFFSEN GLREHLQTHR GPAKHYMCPI CGERFPSLLT LTEHKVTHSK SLDTGTCRIC 

      1030       1040       1050       1060       1070       1080 
KMPLQSEEEF IEHCQMHPDL RNSLTGFRCV VCMQTVTSTL ELKIHGTFHM QKLAGSSAAS 

      1090       1100       1110       1120       1130       1140 
SPNGQGLQKL YKCALCLKEF RSKQDLVRLD VNGLPYGLCA GCMARSANGQ VGGLAPPEPA 

      1150       1160       1170       1180       1190       1200 
DRPCAGLRCP ECNVKFESAE DLESHMQVDH RDLTPETSGP RKGAQTSPVP RKKTYQCIKC 

      1210       1220       1230       1240       1250       1260 
QMTFENEREI QIHVANHMIE EGINHECKLC NQMFDSPAKL LCHLIEHSFE GMGGTFKCPV 

      1270       1280       1290       1300       1310 
CFTVFVQANK LQQHIFAVHG QEDKIYDCSQ CPQKFFFQTE LQNHTMSQHA Q 

« Hide

References

[1]"Zinc finger protein coding cDNA from adult rat aorta."
Patel C.V., Gorski D.H., Walsh K.
Submitted (OCT-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Aorta.
[2]"Cloning and functional characterization of Roaz, a zinc finger protein that interacts with O/E-1 to regulate gene expression: implications for olfactory neuronal development."
Tsai R.Y.L., Reed R.R.
J. Neurosci. 17:4159-4169(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-1311, FUNCTION, DNA-BINDING, TISSUE SPECIFICITY, DOMAIN, INTERACTION WITH EBF1.
Strain: Sprague-Dawley.
[3]"Identification of DNA recognition sequences and protein interaction domains of the multiple-Zn-finger protein Roaz."
Tsai R.Y.L., Reed R.R.
Mol. Cell. Biol. 18:6447-6456(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN, DNA-BINDING, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L03386 mRNA. Translation: AAA42353.1. Frameshift.
U92564 mRNA. Translation: AAB58646.1. Different initiation.
PIRT33754.
T34020.
RefSeqNP_446035.2. NM_053583.2.
UniGeneRn.9981.

3D structure databases

ProteinModelPortalO08961.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid250171. 1 interaction.
STRING10116.ENSRNOP00000020028.

Proteomic databases

PaxDbO08961.
PRIDEO08961.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID94188.
KEGGrno:94188.

Organism-specific databases

CTD94187.
RGD621664. Zfp423.

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000155793.
HOVERGENHBG052773.
InParanoidO08961.
PhylomeDBO08961.

Gene expression databases

GenevestigatorO08961.

Family and domain databases

Gene3D3.30.160.60. 4 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00096. zf-C2H2. 4 hits.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 30 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 27 hits.
PS50157. ZINC_FINGER_C2H2_2. 23 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio617834.
PROO08961.

Entry information

Entry nameZN423_RAT
AccessionPrimary (citable) accession number: O08961
Secondary accession number(s): Q63681
Entry history
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: April 16, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families