Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O08961

- ZN423_RAT

UniProt

O08961 - ZN423_RAT

Protein

Zinc finger protein 423

Gene

Znf423

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (23 Oct 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri75 – 10127C2H2-type 1; degeneratePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri146 – 16823C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri174 – 19623C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri202 – 22423C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri230 – 25223C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri271 – 29424C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri303 – 32624C2H2-type 7PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri331 – 35323C2H2-type 8PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri436 – 46025C2H2-type 9; degeneratePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri468 – 49124C2H2-type 10PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri507 – 53024C2H2-type 11PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri544 – 56724C2H2-type 12PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri590 – 61526C2H2-type 13; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri659 – 68123C2H2-type 14PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri689 – 71123C2H2-type 15PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri719 – 74224C2H2-type 16PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri747 – 77024C2H2-type 17PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri777 – 80024C2H2-type 18PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri808 – 83023C2H2-type 19PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri834 – 85724C2H2-type 20PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri913 – 93523C2H2-type 21; degeneratePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri957 – 97923C2H2-type 22PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri986 – 100823C2H2-type 23PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1047 – 106923C2H2-type 24PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1091 – 110919C2H2-type 25; degeneratePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1147 – 117024C2H2-type 26PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1195 – 121723C2H2-type 27PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1225 – 124723C2H2-type 28PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1256 – 127924C2H2-type 29PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1286 – 130924C2H2-type 30PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein heterodimerization activity Source: RGD
    3. protein homodimerization activity Source: RGD
    4. sequence-specific DNA binding Source: RGD

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. negative regulation of transcription, DNA-templated Source: RGD
    3. nervous system development Source: UniProtKB-KW
    4. Notch signaling pathway Source: UniProtKB
    5. positive regulation of BMP signaling pathway Source: UniProtKB
    6. positive regulation of transcription, DNA-templated Source: UniProtKB
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Developmental protein, Repressor

    Keywords - Biological processi

    Differentiation, Neurogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger protein 423
    Alternative name(s):
    Olf1/EBF-associated zinc finger protein
    Short name:
    rOAZ
    Smad- and Olf-interacting zinc finger protein
    Gene namesi
    Name:Znf423
    Synonyms:Oaz, Zfp423
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621664. Zfp423.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13111311Zinc finger protein 423PRO_0000308597Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei55 – 551PhosphoserineBy similarity
    Modified residuei58 – 581PhosphoserineBy similarity
    Modified residuei631 – 6311PhosphoserineBy similarity
    Modified residuei1081 – 10811PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO08961.
    PRIDEiO08961.

    Expressioni

    Tissue specificityi

    Expressed in brain, eye, olfactory epithelium, spleen and heart. Expressed in the basal layer, consisting of neural precursor cells and immature sensory neurons of the olfactory epithelium, but not in the mature receptor cells.1 Publication

    Gene expression databases

    GenevestigatoriO08961.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with PARP1, SMAD1 and SMAD4 By similarity. Interacts with EBF1. Interacts with CEP290 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi250171. 1 interaction.
    STRINGi10116.ENSRNOP00000020028.

    Structurei

    3D structure databases

    ProteinModelPortaliO08961.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Uses different DNA- and protein-binding zinc fingers to regulate the distinct BMP-Smad and Olf signaling pathways. C2H2-type zinc fingers 14-19 mediate the interaction with SMAD1 and SMAD4, while zinc fingers 28-30 mediate the interaction with EBF1. zinc fingers 2-8 bind the 5'-CCGCCC-3' DNA sequence in concert with EBF1, while zinc fingers 9-13 bind BMP target gene promoters in concert with SMADs By similarity.By similarity

    Sequence similaritiesi

    Contains 30 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri75 – 10127C2H2-type 1; degeneratePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri146 – 16823C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri174 – 19623C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri202 – 22423C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri230 – 25223C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri271 – 29424C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri303 – 32624C2H2-type 7PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri331 – 35323C2H2-type 8PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri436 – 46025C2H2-type 9; degeneratePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri468 – 49124C2H2-type 10PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri507 – 53024C2H2-type 11PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri544 – 56724C2H2-type 12PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri590 – 61526C2H2-type 13; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri659 – 68123C2H2-type 14PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri689 – 71123C2H2-type 15PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri719 – 74224C2H2-type 16PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri747 – 77024C2H2-type 17PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri777 – 80024C2H2-type 18PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri808 – 83023C2H2-type 19PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri834 – 85724C2H2-type 20PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri913 – 93523C2H2-type 21; degeneratePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri957 – 97923C2H2-type 22PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri986 – 100823C2H2-type 23PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1047 – 106923C2H2-type 24PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1091 – 110919C2H2-type 25; degeneratePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1147 – 117024C2H2-type 26PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1195 – 121723C2H2-type 27PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1225 – 124723C2H2-type 28PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1256 – 127924C2H2-type 29PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1286 – 130924C2H2-type 30PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    HOGENOMiHOG000155793.
    HOVERGENiHBG052773.
    InParanoidiO08961.
    PhylomeDBiO08961.

    Family and domain databases

    Gene3Di3.30.160.60. 4 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00096. zf-C2H2. 4 hits.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 30 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 27 hits.
    PS50157. ZINC_FINGER_C2H2_2. 23 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O08961-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRRKQAKPR SVKVEEGEAS DFSLAWDSSV AAAGGLEGES ECDRKSSRAL     50
    EDRNSVTSQE ERNEDDEDVE DESIYTCDHC QQDFESLADL TDHRAHRCPG 100
    DGDDDPQLSW VASSPSSKDV ASPTQMIGDG CDLGLGEEEG GTGLPYPCQF 150
    CDKSFIRLSY LKRHEQIHSD KLPFKCTFCS RLFKHKRSRD RHIKLHTGDK 200
    KYHCHECEAA FSRRDHLKIH LKTHSSSKPF KCSVCKRGFS STSSLQSHMQ 250
    AHKKNKEHLA KSEKEAKKDD FMCDYCEDTF SQTEELEKHV LTLHPQLSEK 300
    ADLQCIHCPE VFVDESTLLA HIHQAHANQK HKCPMCPEQF SSVEGVYCHL 350
    DSHRQPDSSN HSVSPDPVLG SVASMSSATP DSTPDPVLGS VASMSSATPD 400
    SSASVERGST PDSTLKPLRG QKKMRDDGQS WSKVVYSCPY CSKRDFTSLA 450
    VLEIHLKTIH ADKPQQSHTC QICLDSMPTL YNLNEHVRKL HKSHAYPVMQ 500
    FGNISAFHCN YCPEMFADIN SLQEHIRVSH CGPNANPPDG NNAFFCNQCS 550
    MGFLTESSLT EHIQQAHCSV GSTKLESPVI QPTQSFMEVY SCPYCTNSPI 600
    FGSILKLTKH IKENHKNIPL AHSKKSKAEQ SPVSSDVEVS SPKRQRLSGS 650
    ANSISNGEYP CNQCDLKFSN FESFQTHLKL HLELLLRKQA CPQCKEDFDS 700
    QESLLQHLTV HYMTTSTHYV CESCDKQFSS VDDLQKHLLD MHTFVLYHCT 750
    LCQEVFDSKV SIQVHLAVKH SNEKKMYRCT ACNWDFRKEA DLQVHVKHSH 800
    LGNPAKAHKC IFCGETFSTE VELQCHITTH SKKYNCRFCS KAFHAVLLLE 850
    KHLREKHCVF DPAAENGTAN GVPPTSTKKA EPADLQGMLL KNPEAPNSHE 900
    ASEDDVDASE PMYGCDICGA AYTMEVLLQN HRLRDHNIRP GEDDGSRKKA 950
    EFIKGSHKCN VCSRTFFSEN GLREHLQTHR GPAKHYMCPI CGERFPSLLT 1000
    LTEHKVTHSK SLDTGTCRIC KMPLQSEEEF IEHCQMHPDL RNSLTGFRCV 1050
    VCMQTVTSTL ELKIHGTFHM QKLAGSSAAS SPNGQGLQKL YKCALCLKEF 1100
    RSKQDLVRLD VNGLPYGLCA GCMARSANGQ VGGLAPPEPA DRPCAGLRCP 1150
    ECNVKFESAE DLESHMQVDH RDLTPETSGP RKGAQTSPVP RKKTYQCIKC 1200
    QMTFENEREI QIHVANHMIE EGINHECKLC NQMFDSPAKL LCHLIEHSFE 1250
    GMGGTFKCPV CFTVFVQANK LQQHIFAVHG QEDKIYDCSQ CPQKFFFQTE 1300
    LQNHTMSQHA Q 1311
    Length:1,311
    Mass (Da):147,227
    Last modified:October 23, 2007 - v2
    Checksum:i6A5680DA502B7E36
    GO

    Sequence cautioni

    The sequence AAA42353.1 differs from that shown. Reason: Frameshift at several positions.
    The sequence AAB58646.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L03386 mRNA. Translation: AAA42353.1. Frameshift.
    U92564 mRNA. Translation: AAB58646.1. Different initiation.
    PIRiT33754.
    T34020.
    RefSeqiNP_446035.2. NM_053583.2.
    UniGeneiRn.9981.

    Genome annotation databases

    GeneIDi94188.
    KEGGirno:94188.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L03386 mRNA. Translation: AAA42353.1 . Frameshift.
    U92564 mRNA. Translation: AAB58646.1 . Different initiation.
    PIRi T33754.
    T34020.
    RefSeqi NP_446035.2. NM_053583.2.
    UniGenei Rn.9981.

    3D structure databases

    ProteinModelPortali O08961.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250171. 1 interaction.
    STRINGi 10116.ENSRNOP00000020028.

    Proteomic databases

    PaxDbi O08961.
    PRIDEi O08961.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 94188.
    KEGGi rno:94188.

    Organism-specific databases

    CTDi 94187.
    RGDi 621664. Zfp423.

    Phylogenomic databases

    eggNOGi COG5048.
    HOGENOMi HOG000155793.
    HOVERGENi HBG052773.
    InParanoidi O08961.
    PhylomeDBi O08961.

    Miscellaneous databases

    NextBioi 617834.
    PROi O08961.

    Gene expression databases

    Genevestigatori O08961.

    Family and domain databases

    Gene3Di 3.30.160.60. 4 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00096. zf-C2H2. 4 hits.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 30 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 27 hits.
    PS50157. ZINC_FINGER_C2H2_2. 23 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Zinc finger protein coding cDNA from adult rat aorta."
      Patel C.V., Gorski D.H., Walsh K.
      Submitted (OCT-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Aorta.
    2. "Cloning and functional characterization of Roaz, a zinc finger protein that interacts with O/E-1 to regulate gene expression: implications for olfactory neuronal development."
      Tsai R.Y.L., Reed R.R.
      J. Neurosci. 17:4159-4169(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-1311, FUNCTION, DNA-BINDING, TISSUE SPECIFICITY, DOMAIN, INTERACTION WITH EBF1.
      Strain: Sprague-Dawley.
    3. "Identification of DNA recognition sequences and protein interaction domains of the multiple-Zn-finger protein Roaz."
      Tsai R.Y.L., Reed R.R.
      Mol. Cell. Biol. 18:6447-6456(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN, DNA-BINDING, SUBUNIT.

    Entry informationi

    Entry nameiZN423_RAT
    AccessioniPrimary (citable) accession number: O08961
    Secondary accession number(s): Q63681
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 2007
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3