SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O08961

- ZN423_RAT

UniProt

O08961 - ZN423_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Zinc finger protein 423

Gene
Znf423, Oaz, Zfp423
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri75 – 10127C2H2-type 1; degenerateAdd
BLAST
Zinc fingeri146 – 16823C2H2-type 2Add
BLAST
Zinc fingeri174 – 19623C2H2-type 3Add
BLAST
Zinc fingeri202 – 22423C2H2-type 4Add
BLAST
Zinc fingeri230 – 25223C2H2-type 5Add
BLAST
Zinc fingeri271 – 29424C2H2-type 6Add
BLAST
Zinc fingeri303 – 32624C2H2-type 7Add
BLAST
Zinc fingeri331 – 35323C2H2-type 8Add
BLAST
Zinc fingeri436 – 46025C2H2-type 9; degenerateAdd
BLAST
Zinc fingeri468 – 49124C2H2-type 10Add
BLAST
Zinc fingeri507 – 53024C2H2-type 11Add
BLAST
Zinc fingeri544 – 56724C2H2-type 12Add
BLAST
Zinc fingeri590 – 61526C2H2-type 13; atypicalAdd
BLAST
Zinc fingeri659 – 68123C2H2-type 14Add
BLAST
Zinc fingeri689 – 71123C2H2-type 15Add
BLAST
Zinc fingeri719 – 74224C2H2-type 16Add
BLAST
Zinc fingeri747 – 77024C2H2-type 17Add
BLAST
Zinc fingeri777 – 80024C2H2-type 18Add
BLAST
Zinc fingeri808 – 83023C2H2-type 19Add
BLAST
Zinc fingeri834 – 85724C2H2-type 20Add
BLAST
Zinc fingeri913 – 93523C2H2-type 21; degenerateAdd
BLAST
Zinc fingeri957 – 97923C2H2-type 22Add
BLAST
Zinc fingeri986 – 100823C2H2-type 23Add
BLAST
Zinc fingeri1047 – 106923C2H2-type 24Add
BLAST
Zinc fingeri1091 – 110919C2H2-type 25; degenerateAdd
BLAST
Zinc fingeri1147 – 117024C2H2-type 26Add
BLAST
Zinc fingeri1195 – 121723C2H2-type 27Add
BLAST
Zinc fingeri1225 – 124723C2H2-type 28Add
BLAST
Zinc fingeri1256 – 127924C2H2-type 29Add
BLAST
Zinc fingeri1286 – 130924C2H2-type 30Add
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein heterodimerization activity Source: RGD
  3. protein homodimerization activity Source: RGD
  4. sequence-specific DNA binding Source: RGD

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. negative regulation of transcription, DNA-templated Source: RGD
  3. nervous system development Source: UniProtKB-KW
  4. Notch signaling pathway Source: UniProtKB
  5. positive regulation of BMP signaling pathway Source: UniProtKB
  6. positive regulation of transcription, DNA-templated Source: UniProtKB
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 423
Alternative name(s):
Olf1/EBF-associated zinc finger protein
Short name:
rOAZ
Smad- and Olf-interacting zinc finger protein
Gene namesi
Name:Znf423
Synonyms:Oaz, Zfp423
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621664. Zfp423.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13111311Zinc finger protein 423PRO_0000308597Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551Phosphoserine By similarity
Modified residuei58 – 581Phosphoserine By similarity
Modified residuei631 – 6311Phosphoserine By similarity
Modified residuei1081 – 10811Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO08961.
PRIDEiO08961.

Expressioni

Tissue specificityi

Expressed in brain, eye, olfactory epithelium, spleen and heart. Expressed in the basal layer, consisting of neural precursor cells and immature sensory neurons of the olfactory epithelium, but not in the mature receptor cells.1 Publication

Gene expression databases

GenevestigatoriO08961.

Interactioni

Subunit structurei

Homodimer. Interacts with PARP1, SMAD1 and SMAD4 By similarity. Interacts with EBF1. Interacts with CEP290 By similarity.2 Publications

Protein-protein interaction databases

BioGridi250171. 1 interaction.
STRINGi10116.ENSRNOP00000020028.

Structurei

3D structure databases

ProteinModelPortaliO08961.

Family & Domainsi

Domaini

Uses different DNA- and protein-binding zinc fingers to regulate the distinct BMP-Smad and Olf signaling pathways. C2H2-type zinc fingers 14-19 mediate the interaction with SMAD1 and SMAD4, while zinc fingers 28-30 mediate the interaction with EBF1. zinc fingers 2-8 bind the 5'-CCGCCC-3' DNA sequence in concert with EBF1, while zinc fingers 9-13 bind BMP target gene promoters in concert with SMADs By similarity.2 Publications

Sequence similaritiesi

Contains 30 C2H2-type zinc fingers.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri75 – 10127C2H2-type 1; degenerateAdd
BLAST
Zinc fingeri146 – 16823C2H2-type 2Add
BLAST
Zinc fingeri174 – 19623C2H2-type 3Add
BLAST
Zinc fingeri202 – 22423C2H2-type 4Add
BLAST
Zinc fingeri230 – 25223C2H2-type 5Add
BLAST
Zinc fingeri271 – 29424C2H2-type 6Add
BLAST
Zinc fingeri303 – 32624C2H2-type 7Add
BLAST
Zinc fingeri331 – 35323C2H2-type 8Add
BLAST
Zinc fingeri436 – 46025C2H2-type 9; degenerateAdd
BLAST
Zinc fingeri468 – 49124C2H2-type 10Add
BLAST
Zinc fingeri507 – 53024C2H2-type 11Add
BLAST
Zinc fingeri544 – 56724C2H2-type 12Add
BLAST
Zinc fingeri590 – 61526C2H2-type 13; atypicalAdd
BLAST
Zinc fingeri659 – 68123C2H2-type 14Add
BLAST
Zinc fingeri689 – 71123C2H2-type 15Add
BLAST
Zinc fingeri719 – 74224C2H2-type 16Add
BLAST
Zinc fingeri747 – 77024C2H2-type 17Add
BLAST
Zinc fingeri777 – 80024C2H2-type 18Add
BLAST
Zinc fingeri808 – 83023C2H2-type 19Add
BLAST
Zinc fingeri834 – 85724C2H2-type 20Add
BLAST
Zinc fingeri913 – 93523C2H2-type 21; degenerateAdd
BLAST
Zinc fingeri957 – 97923C2H2-type 22Add
BLAST
Zinc fingeri986 – 100823C2H2-type 23Add
BLAST
Zinc fingeri1047 – 106923C2H2-type 24Add
BLAST
Zinc fingeri1091 – 110919C2H2-type 25; degenerateAdd
BLAST
Zinc fingeri1147 – 117024C2H2-type 26Add
BLAST
Zinc fingeri1195 – 121723C2H2-type 27Add
BLAST
Zinc fingeri1225 – 124723C2H2-type 28Add
BLAST
Zinc fingeri1256 – 127924C2H2-type 29Add
BLAST
Zinc fingeri1286 – 130924C2H2-type 30Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
HOGENOMiHOG000155793.
HOVERGENiHBG052773.
InParanoidiO08961.
PhylomeDBiO08961.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 4 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 30 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 27 hits.
PS50157. ZINC_FINGER_C2H2_2. 23 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08961-1 [UniParc]FASTAAdd to Basket

« Hide

MSRRKQAKPR SVKVEEGEAS DFSLAWDSSV AAAGGLEGES ECDRKSSRAL     50
EDRNSVTSQE ERNEDDEDVE DESIYTCDHC QQDFESLADL TDHRAHRCPG 100
DGDDDPQLSW VASSPSSKDV ASPTQMIGDG CDLGLGEEEG GTGLPYPCQF 150
CDKSFIRLSY LKRHEQIHSD KLPFKCTFCS RLFKHKRSRD RHIKLHTGDK 200
KYHCHECEAA FSRRDHLKIH LKTHSSSKPF KCSVCKRGFS STSSLQSHMQ 250
AHKKNKEHLA KSEKEAKKDD FMCDYCEDTF SQTEELEKHV LTLHPQLSEK 300
ADLQCIHCPE VFVDESTLLA HIHQAHANQK HKCPMCPEQF SSVEGVYCHL 350
DSHRQPDSSN HSVSPDPVLG SVASMSSATP DSTPDPVLGS VASMSSATPD 400
SSASVERGST PDSTLKPLRG QKKMRDDGQS WSKVVYSCPY CSKRDFTSLA 450
VLEIHLKTIH ADKPQQSHTC QICLDSMPTL YNLNEHVRKL HKSHAYPVMQ 500
FGNISAFHCN YCPEMFADIN SLQEHIRVSH CGPNANPPDG NNAFFCNQCS 550
MGFLTESSLT EHIQQAHCSV GSTKLESPVI QPTQSFMEVY SCPYCTNSPI 600
FGSILKLTKH IKENHKNIPL AHSKKSKAEQ SPVSSDVEVS SPKRQRLSGS 650
ANSISNGEYP CNQCDLKFSN FESFQTHLKL HLELLLRKQA CPQCKEDFDS 700
QESLLQHLTV HYMTTSTHYV CESCDKQFSS VDDLQKHLLD MHTFVLYHCT 750
LCQEVFDSKV SIQVHLAVKH SNEKKMYRCT ACNWDFRKEA DLQVHVKHSH 800
LGNPAKAHKC IFCGETFSTE VELQCHITTH SKKYNCRFCS KAFHAVLLLE 850
KHLREKHCVF DPAAENGTAN GVPPTSTKKA EPADLQGMLL KNPEAPNSHE 900
ASEDDVDASE PMYGCDICGA AYTMEVLLQN HRLRDHNIRP GEDDGSRKKA 950
EFIKGSHKCN VCSRTFFSEN GLREHLQTHR GPAKHYMCPI CGERFPSLLT 1000
LTEHKVTHSK SLDTGTCRIC KMPLQSEEEF IEHCQMHPDL RNSLTGFRCV 1050
VCMQTVTSTL ELKIHGTFHM QKLAGSSAAS SPNGQGLQKL YKCALCLKEF 1100
RSKQDLVRLD VNGLPYGLCA GCMARSANGQ VGGLAPPEPA DRPCAGLRCP 1150
ECNVKFESAE DLESHMQVDH RDLTPETSGP RKGAQTSPVP RKKTYQCIKC 1200
QMTFENEREI QIHVANHMIE EGINHECKLC NQMFDSPAKL LCHLIEHSFE 1250
GMGGTFKCPV CFTVFVQANK LQQHIFAVHG QEDKIYDCSQ CPQKFFFQTE 1300
LQNHTMSQHA Q 1311
Length:1,311
Mass (Da):147,227
Last modified:October 23, 2007 - v2
Checksum:i6A5680DA502B7E36
GO

Sequence cautioni

The sequence AAA42353.1 differs from that shown. Reason: Frameshift at several positions.
The sequence AAB58646.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L03386 mRNA. Translation: AAA42353.1. Frameshift.
U92564 mRNA. Translation: AAB58646.1. Different initiation.
PIRiT33754.
T34020.
RefSeqiNP_446035.2. NM_053583.2.
UniGeneiRn.9981.

Genome annotation databases

GeneIDi94188.
KEGGirno:94188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L03386 mRNA. Translation: AAA42353.1 . Frameshift.
U92564 mRNA. Translation: AAB58646.1 . Different initiation.
PIRi T33754.
T34020.
RefSeqi NP_446035.2. NM_053583.2.
UniGenei Rn.9981.

3D structure databases

ProteinModelPortali O08961.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250171. 1 interaction.
STRINGi 10116.ENSRNOP00000020028.

Proteomic databases

PaxDbi O08961.
PRIDEi O08961.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 94188.
KEGGi rno:94188.

Organism-specific databases

CTDi 94187.
RGDi 621664. Zfp423.

Phylogenomic databases

eggNOGi COG5048.
HOGENOMi HOG000155793.
HOVERGENi HBG052773.
InParanoidi O08961.
PhylomeDBi O08961.

Miscellaneous databases

NextBioi 617834.
PROi O08961.

Gene expression databases

Genevestigatori O08961.

Family and domain databases

Gene3Di 3.30.160.60. 4 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF00096. zf-C2H2. 4 hits.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 30 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 27 hits.
PS50157. ZINC_FINGER_C2H2_2. 23 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Zinc finger protein coding cDNA from adult rat aorta."
    Patel C.V., Gorski D.H., Walsh K.
    Submitted (OCT-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Aorta.
  2. "Cloning and functional characterization of Roaz, a zinc finger protein that interacts with O/E-1 to regulate gene expression: implications for olfactory neuronal development."
    Tsai R.Y.L., Reed R.R.
    J. Neurosci. 17:4159-4169(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-1311, FUNCTION, DNA-BINDING, TISSUE SPECIFICITY, DOMAIN, INTERACTION WITH EBF1.
    Strain: Sprague-Dawley.
  3. "Identification of DNA recognition sequences and protein interaction domains of the multiple-Zn-finger protein Roaz."
    Tsai R.Y.L., Reed R.R.
    Mol. Cell. Biol. 18:6447-6456(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN, DNA-BINDING, SUBUNIT.

Entry informationi

Entry nameiZN423_RAT
AccessioniPrimary (citable) accession number: O08961
Secondary accession number(s): Q63681
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: April 16, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi