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Protein

Zinc finger protein SNAI2

Gene

Snai2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri128 – 15023C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri159 – 18123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri185 – 20723C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri213 – 23523C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri241 – 26424C2H2-type 5; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein SNAI2
Alternative name(s):
Neural crest transcription factor Slug
Protein snail homolog 2
Gene namesi
Name:Snai2
Synonyms:Slug, Slugh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi3722. Snai2.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Zinc finger protein SNAI2PRO_0000047034Add
BLAST

Post-translational modificationi

GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation.By similarity

Proteomic databases

PaxDbiO08954.

Expressioni

Gene expression databases

GenevisibleiO08954. RN.

Interactioni

Subunit structurei

Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains) (By similarity). Interacts (via zinc fingers) with KPNA2, KPNB1 and TNPO1. May interact (via zinc fingers) with IPO7 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064195.

Structurei

3D structure databases

ProteinModelPortaliO08954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2020SNAG domainBy similarityAdd
BLAST

Domaini

Repression activity depends on the C-terminal DNA-binding zinc fingers and on the N-terminal repression domain.By similarity

Sequence similaritiesi

Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri128 – 15023C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri159 – 18123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri185 – 20723C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri213 – 23523C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri241 – 26424C2H2-type 5; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2462. Eukaryota.
ENOG41106JS. LUCA.
GeneTreeiENSGT00390000011027.
HOVERGENiHBG007477.
InParanoidiO08954.
KOiK05706.
OMAiVIPQPEV.
OrthoDBiEOG7P2XSG.
PhylomeDBiO08954.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 2 hits.
PF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRSFLVKKH FNASKKPNYS ELDTHTVIIS PYLCESYPMP VIPKPEILTS
60 70 80 90 100
GAYSPITVWT SAVPFHSPLP SGLSPLTGYS SSLGRVSPLP SSDTSSKDHS
110 120 130 140 150
GSESPISDEE ERLQPKLSDP HAIEAEKFQC NLCNKTYSTF SGLAKHKQLH
160 170 180 190 200
CDAQARKSFS CKYCDKEYVS LGALKMHIRT HTLPCVCKIC GKAFSRPWLL
210 220 230 240 250
QGHIRTHTGE KPFSCPHCNR AFADRSNLRA HLQTHSDVKK YQCKNCSKTF
260
SRMSLLHKHE ESGCCVAH
Length:268
Mass (Da):29,936
Last modified:December 6, 2005 - v2
Checksum:i92B6325E54CEFD4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF497973 mRNA. Translation: AAM19227.1.
U97061 mRNA. Translation: AAB58706.1.
RefSeqiNP_037167.1. NM_013035.1.
UniGeneiRn.43117.

Genome annotation databases

EnsembliENSRNOT00000073049; ENSRNOP00000064195; ENSRNOG00000047699.
GeneIDi25554.
KEGGirno:25554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF497973 mRNA. Translation: AAM19227.1.
U97061 mRNA. Translation: AAB58706.1.
RefSeqiNP_037167.1. NM_013035.1.
UniGeneiRn.43117.

3D structure databases

ProteinModelPortaliO08954.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064195.

Proteomic databases

PaxDbiO08954.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000073049; ENSRNOP00000064195; ENSRNOG00000047699.
GeneIDi25554.
KEGGirno:25554.

Organism-specific databases

CTDi6591.
RGDi3722. Snai2.

Phylogenomic databases

eggNOGiKOG2462. Eukaryota.
ENOG41106JS. LUCA.
GeneTreeiENSGT00390000011027.
HOVERGENiHBG007477.
InParanoidiO08954.
KOiK05706.
OMAiVIPQPEV.
OrthoDBiEOG7P2XSG.
PhylomeDBiO08954.

Miscellaneous databases

PROiO08954.

Gene expression databases

GenevisibleiO08954. RN.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 2 hits.
PF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Regulation of insulin producing beta-cells survival by SLUG, a zinc-finger transcription factor, with homology to CES-1."
    Ubeda M., Habener J.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The zinc-finger protein Slug causes desmosome dissociation, an initial and necessary step for growth factor-induced epithelial-mesenchymal transition."
    Savagner P., Yamada K.M., Thiery J.P.
    J. Cell Biol. 137:1403-1419(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 23-172.
  3. "Raf 1 represses expression of the tight junction protein occludin via activation of the zinc-finger transcription factor slug."
    Wang Z., Wade P., Mandell K.J., Akyildiz A., Parkos C.A., Mrsny R.J., Nusrat A.
    Oncogene 26:1222-1230(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSNAI2_RAT
AccessioniPrimary (citable) accession number: O08954
Secondary accession number(s): Q8R482
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 6, 2005
Last modified: June 8, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.