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Protein

Transcription initiation factor IIA subunit 1

Gene

Gtf2a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-6807505. RNA polymerase II transcribes snRNA genes.
R-RNO-73776. RNA Polymerase II Promoter Escape.
R-RNO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-RNO-75953. RNA Polymerase II Transcription Initiation.
R-RNO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIA subunit 1
Alternative name(s):
General transcription factor IIA subunit 1
Cleaved into the following 2 chains:
Alternative name(s):
TFIIA p35 subunit
Alternative name(s):
TFIIA p19 subunit
Gene namesi
Name:Gtf2a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi69246. Gtf2a1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 377376Transcription initiation factor IIA subunit 1PRO_0000042601Add
BLAST
Chaini2 – 275274Transcription initiation factor IIA alpha chainPRO_0000042602Add
BLAST
Chaini276 – 377102Transcription initiation factor IIA beta chainPRO_0000042603Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei281 – 2811Phosphoserine; by TAF1By similarity
Modified residuei282 – 2821Phosphoserine; by TAF1By similarity
Modified residuei317 – 3171PhosphoserineCombined sources
Modified residuei322 – 3221PhosphoserineCombined sources

Post-translational modificationi

The alpha and beta subunits are postranslationally produced from the precursor formby TASP1. The cleavage promotes proteasomal degradation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei275 – 2762Cleavage; by TASP1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO08949.
PRIDEiO08949.

PTM databases

iPTMnetiO08949.
PhosphoSiteiO08949.

Expressioni

Gene expression databases

GenevisibleiO08949. RN.

Interactioni

Subunit structurei

TFIIA is a heterodimer of a unprocessed large subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha, a beta and a gamma subunit. TFIIA forms a complex with TBP (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005873.

Structurei

3D structure databases

ProteinModelPortaliO08949.
SMRiO08949. Positions 9-51, 331-377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi71 – 8010Poly-Gln
Compositional biasi81 – 877Poly-His

Sequence similaritiesi

Belongs to the TFIIA subunit 1 family.Curated

Phylogenomic databases

eggNOGiKOG2652. Eukaryota.
ENOG4111M8K. LUCA.
GeneTreeiENSGT00830000128302.
HOGENOMiHOG000015236.
HOVERGENiHBG052771.
InParanoidiO08949.
KOiK03122.
OMAiTHHWERF.
OrthoDBiEOG7KWSJM.
PhylomeDBiO08949.
TreeFamiTF350445.

Family and domain databases

Gene3Di1.10.287.100. 1 hit.
2.30.18.10. 1 hit.
InterProiIPR009083. TFIIA_a-hlx.
IPR004855. TFIIA_asu/bsu.
IPR013028. TFIIA_asu_N.
IPR009088. TFIIA_b-brl.
[Graphical view]
PANTHERiPTHR12694. PTHR12694. 1 hit.
PfamiPF03153. TFIIA. 2 hits.
[Graphical view]
SMARTiSM01371. TFIIA. 1 hit.
[Graphical view]
SUPFAMiSSF47396. SSF47396. 1 hit.
SSF50784. SSF50784. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08949-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANSANTNTV PKLYRSVIED VINDVRDIFL DDGVDEQVLM ELKTLWENKL
60 70 80 90 100
MQSRAVDGFH SEEQQLLLQV QQQHQPQQQQ HHHHHHHQQA QPQQTVPQQA
110 120 130 140 150
QTQQVLIPAS QQATAPQVIV PDSKLIQHMN ASSITSAAAT AATLALPAGV
160 170 180 190 200
TPVQQILTNS GQLLQVVRAA NGAQYIFQPQ QSVVLQQQVI PQMQPGGVQA
210 220 230 240 250
PVIQQVLAPL PGGISPQTGV IIQPQQILFT GNKTQVIPTT VAAPTPAQAQ
260 270 280 290 300
IPAAGQQQPQ AQPAQQQAPL VLQVDGTGDT SSEEDEDEEE DYDDDEEEDK
310 320 330 340 350
EKDGAEDGQV EEEPLNSEDD VSDEEGQELF DTENVVVCQY DKIHRSKNKW
360 370
KFHLKDGIMN LNGRDYIFSK AIGDAEW
Length:377
Mass (Da):41,563
Last modified:July 1, 1997 - v1
Checksum:i205FEEB2B24E8D71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000943 mRNA. Translation: AAB58716.1.
RefSeqiNP_071544.1. NM_022208.1.
UniGeneiRn.24468.
Rn.44436.

Genome annotation databases

EnsembliENSRNOT00000005873; ENSRNOP00000005873; ENSRNOG00000004300.
GeneIDi83830.
KEGGirno:83830.
UCSCiRGD:69246. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000943 mRNA. Translation: AAB58716.1.
RefSeqiNP_071544.1. NM_022208.1.
UniGeneiRn.24468.
Rn.44436.

3D structure databases

ProteinModelPortaliO08949.
SMRiO08949. Positions 9-51, 331-377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005873.

PTM databases

iPTMnetiO08949.
PhosphoSiteiO08949.

Proteomic databases

PaxDbiO08949.
PRIDEiO08949.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005873; ENSRNOP00000005873; ENSRNOG00000004300.
GeneIDi83830.
KEGGirno:83830.
UCSCiRGD:69246. rat.

Organism-specific databases

CTDi2957.
RGDi69246. Gtf2a1.

Phylogenomic databases

eggNOGiKOG2652. Eukaryota.
ENOG4111M8K. LUCA.
GeneTreeiENSGT00830000128302.
HOGENOMiHOG000015236.
HOVERGENiHBG052771.
InParanoidiO08949.
KOiK03122.
OMAiTHHWERF.
OrthoDBiEOG7KWSJM.
PhylomeDBiO08949.
TreeFamiTF350445.

Enzyme and pathway databases

ReactomeiR-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-6807505. RNA polymerase II transcribes snRNA genes.
R-RNO-73776. RNA Polymerase II Promoter Escape.
R-RNO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-RNO-75953. RNA Polymerase II Transcription Initiation.
R-RNO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

PROiO08949.

Gene expression databases

GenevisibleiO08949. RN.

Family and domain databases

Gene3Di1.10.287.100. 1 hit.
2.30.18.10. 1 hit.
InterProiIPR009083. TFIIA_a-hlx.
IPR004855. TFIIA_asu/bsu.
IPR013028. TFIIA_asu_N.
IPR009088. TFIIA_b-brl.
[Graphical view]
PANTHERiPTHR12694. PTHR12694. 1 hit.
PfamiPF03153. TFIIA. 2 hits.
[Graphical view]
SMARTiSM01371. TFIIA. 1 hit.
[Graphical view]
SUPFAMiSSF47396. SSF47396. 1 hit.
SSF50784. SSF50784. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "TFIIAalpha/beta-like factor is encoded by a germ cell-specific gene whose expression is up-regulated with other general transcription factors during spermatogenesis in the mouse."
    Han S., Zhou L., Upadhyaya A.B., Lee S.H., Parker K.L., DeJong J.
    Biol. Reprod. 64:507-517(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-317 AND SER-322, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTF2AA_RAT
AccessioniPrimary (citable) accession number: O08949
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 1, 1997
Last modified: June 8, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.