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Protein

Homeobox protein unc-4 homolog

Gene

Uncx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor involved in somitogenesis and neurogenesis. Required for the maintenance and differentiation of particular elements of the axial skeleton. May act upstream of PAX9. Plays a role in controlling the development of connections of hypothalamic neurons to pituitary elements, allowing central neurons to reach the peripheral blood circulation and to deliver hormones for control of peripheral functions.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi109 – 16860HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cartilage condensation Source: MGI
  • common myeloid progenitor cell proliferation Source: MGI
  • dorsal spinal cord development Source: MGI
  • olfactory bulb interneuron differentiation Source: MGI
  • pattern specification process Source: MGI
  • regulation of cell differentiation Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein unc-4 homolog
Alternative name(s):
Homeobox protein Uncx4.1
Gene namesi
Name:Uncx
Synonyms:Uncx4.1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:108013. Uncx.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice die perinatally and exhibit severe malformations of the axial skeleton. Pedicles of the neural arches and proximal ribs are not formed. In addition, dorsal root ganglia are disorganized. In the hypothalamo-neurohypophysial system, neurons are viable and able to express neuropeptides; however, the connectivity of magnocellular neurons with posterior pituitary elements is compromised.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530Homeobox protein unc-4 homologPRO_0000334625Add
BLAST

Proteomic databases

MaxQBiO08934.
PaxDbiO08934.
PRIDEiO08934.

PTM databases

PhosphoSiteiO08934.

Expressioni

Tissue specificityi

Expressed in the paraxial mesoderm, in the developing kidney and central nervous system. In the somite, it is restricted to the caudal half of the newly formed somite and sclerotome. In the central nervous system, it is detected in the developing spinal cord, hindbrain, mesencephalon and telencephalon. Expressed in adult and embryonic magnocellular neurons of the hypothalamo-neurohypophysial system.9 Publications

Gene expression databases

BgeeiO08934.
ExpressionAtlasiO08934. baseline and differential.
GenevisibleiO08934. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031523.

Structurei

3D structure databases

ProteinModelPortaliO08934.
SMRiO08934. Positions 116-165.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi248 – 522275Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the paired homeobox family. Unc-4 subfamily.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00830000128306.
HOGENOMiHOG000154780.
HOVERGENiHBG096185.
InParanoidiO08934.
KOiK09328.
OMAiSCDQTPH.
PhylomeDBiO08934.
TreeFamiTF315554.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08934-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMDGRLLEHP HAQFGGSLGG VVGFPYPLGH HHVYELAGHQ LQSAAAAAAA
60 70 80 90 100
ASVPFSIDGL LSGSCAAAAA SVVNPTPLLP AACGVAGESQ PFKLADSGDP
110 120 130 140 150
DKESPGCKRR RTRTNFTGWQ LEELEKAFNE SHYPDVFMRE ALALRLDLVE
160 170 180 190 200
SRVQVWFQNR RAKWRKKENT KKGPGRPAHN SHPTTCSGEP MDPEEIARKE
210 220 230 240 250
LEKMEKKKRK HEKKLLKSQS RHLHSPGGLS LHSAPSSDSD SGGGGLSPEP
260 270 280 290 300
PEPPPPTAAA KGPGAHGSGI AGSAPVPPGE PPAPGTCDPA FYPSQRSGAG
310 320 330 340 350
SQPRLGRPAD KDTVPCGPGA AATAGLPKAS PFSVESLLSD SPPRRKATPA
360 370 380 390 400
NAAATAGLDF TPGLPCAPRT LIGKGHFLLY PITQPLGFLV PQAALKGGAG
410 420 430 440 450
PELVPKDAPP APPAPPAPPA QASFGTFPGP GGAADPAFAR RSPEVVASPG
460 470 480 490 500
PPAPASFRDL TAAAAESGAG DCADVGTVCP AASPPPPLET SPGPGPRAPS
510 520 530
PPGEPATCGA AEPGAATGPS PPEGEEVDMD
Length:530
Mass (Da):53,936
Last modified:November 1, 1998 - v2
Checksum:i2E3A4DE98428E381
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti260 – 2601A → D in AAC52830 (PubMed:8855241).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001116 mRNA. Translation: CAA04542.1.
AF247550 mRNA. Translation: AAF71322.1.
BC051973 mRNA. Translation: AAH51973.1.
Z96107 mRNA. Translation: CAB09537.1.
AH006754 Genomic DNA. Translation: AAC52830.1.
CCDSiCCDS19813.1.
RefSeqiNP_038730.1. NM_013702.3.
UniGeneiMm.42190.

Genome annotation databases

EnsembliENSMUST00000172997; ENSMUSP00000134067; ENSMUSG00000029546.
GeneIDi22255.
KEGGimmu:22255.
UCSCiuc009agv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001116 mRNA. Translation: CAA04542.1.
AF247550 mRNA. Translation: AAF71322.1.
BC051973 mRNA. Translation: AAH51973.1.
Z96107 mRNA. Translation: CAB09537.1.
AH006754 Genomic DNA. Translation: AAC52830.1.
CCDSiCCDS19813.1.
RefSeqiNP_038730.1. NM_013702.3.
UniGeneiMm.42190.

3D structure databases

ProteinModelPortaliO08934.
SMRiO08934. Positions 116-165.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031523.

PTM databases

PhosphoSiteiO08934.

Proteomic databases

MaxQBiO08934.
PaxDbiO08934.
PRIDEiO08934.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000172997; ENSMUSP00000134067; ENSMUSG00000029546.
GeneIDi22255.
KEGGimmu:22255.
UCSCiuc009agv.1. mouse.

Organism-specific databases

CTDi340260.
MGIiMGI:108013. Uncx.

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00830000128306.
HOGENOMiHOG000154780.
HOVERGENiHBG096185.
InParanoidiO08934.
KOiK09328.
OMAiSCDQTPH.
PhylomeDBiO08934.
TreeFamiTF315554.

Miscellaneous databases

NextBioi302335.
PROiO08934.
SOURCEiSearch...

Gene expression databases

BgeeiO08934.
ExpressionAtlasiO08934. baseline and differential.
GenevisibleiO08934. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The paired homeobox gene Uncx4.1 specifies pedicles, transverse processes and proximal ribs of the vertebral column."
    Leitges M., Neidhardt L., Haenig B., Herrmann B.G., Kispert A.
    Development 127:2259-2267(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE.
  2. "Cloning and expression of the mouse homolog of Caenorhabditis elegans unc4 gene."
    Nicholson L.F.B., Ma L., Goulding M.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spinal cord.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Paired-related murine homeobox gene expressed in the developing sclerotome, kidney, and nervous system."
    Mansouri A., Yokota Y., Wehr R., Copeland N.G., Jenkins N.A., Gruss P.
    Dev. Dyn. 210:53-65(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-387, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6J.
  5. "Cloning and characterization of four murine homeobox genes."
    Rovescalli A.C., Asoh S., Nirenberg M.W.
    Proc. Natl. Acad. Sci. U.S.A. 93:10691-10696(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 109-304.
  6. "Interaction between Notch signalling and Lunatic fringe during somite boundary formation in the mouse."
    del Barco Barrantes I., Elia A.J., Wuensch K., De Angelis M.H., Mak T.W., Rossant J., Conlon R.A., Gossler A., de la Pompa J.L.
    Curr. Biol. 9:470-480(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Adhesive subdivisions intrinsic to the epithelial somites."
    Horikawa K., Radice G., Takeichi M., Chisaka O.
    Dev. Biol. 215:182-189(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Uncx4.1 is required for the formation of the pedicles and proximal ribs and acts upstream of Pax9."
    Mansouri A., Voss A.K., Thomas T., Yokota Y., Gruss P.
    Development 127:2251-2258(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  9. "Early mesodermal phenotypes in splotch suggest a role for Pax3 in the formation of epithelial somites."
    Schubert F.R., Tremblay P., Mansouri A., Faisst A.M., Kammandel B., Lumsden A., Gruss P., Dietrich S.
    Dev. Dyn. 222:506-521(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  10. "Hypomorphic Mesp allele distinguishes establishment of rostrocaudal polarity and segment border formation in somitogenesis."
    Nomura-Kitabayashi A., Takahashi Y., Kitajima S., Inoue T., Takeda H., Saga Y.
    Development 129:2473-2481(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  11. "Identification of Epha4 enhancer required for segmental expression and the regulation by Mesp2."
    Nakajima Y., Morimoto M., Takahashi Y., Koseki H., Saga Y.
    Development 133:2517-2525(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  12. "Neurohypophysial dysmorphogenesis in mice lacking the homeobox gene Uncx4.1."
    Asbreuk C.H.J., van Doorninck J.H., Mansouri A., Smidt M.P., Burbach J.P.H.
    J. Mol. Endocrinol. 36:65-71(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  13. "Transcription factors Mesp2 and Paraxis have critical roles in axial musculoskeletal formation."
    Takahashi Y., Takagi A., Hiraoka S., Koseki H., Kanno J., Rawls A., Saga Y.
    Dev. Dyn. 236:1484-1494(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  14. "Ripply2 is essential for precise somite formation during mouse early development."
    Chan T., Kondow A., Hosoya A., Hitachi K., Yukita A., Okabayashi K., Nakamura H., Ozawa H., Kiyonari H., Michiue T., Ito Y., Asashima M.
    FEBS Lett. 581:2691-2696(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiUNC4_MOUSE
AccessioniPrimary (citable) accession number: O08934
Secondary accession number(s): P70457, Q78DU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 1, 1998
Last modified: February 17, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Marker of antero-posterior subdivisions of the somite.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.