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Protein

Fatty-acid amide hydrolase 1

Gene

Faah

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (By similarity).By similarity

Catalytic activityi

Anandamide + H2O = arachidonic acid + ethanolamine.
Oleamide + H2O = oleic acid + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei142Charge relay systemBy similarity1
Binding sitei191Substrate; via carbonyl oxygenBy similarity1
Active sitei217Charge relay systemBy similarity1
Binding sitei217SubstrateBy similarity1
Active sitei241Acyl-ester intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.5.1.4. 3474.
3.5.1.99. 3474.
ReactomeiR-MMU-2142753. Arachidonic acid metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty-acid amide hydrolase 1 (EC:3.5.1.99)
Alternative name(s):
Anandamide amidohydrolase 1
Oleamide hydrolase 1
Gene namesi
Name:Faah
Synonyms:Faah1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:109609. Faah.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 403CytoplasmicBy similarityAdd BLAST374
Intramembranei404 – 433By similarityAdd BLAST30
Topological domaini434 – 579CytoplasmicBy similarityAdd BLAST146

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3455.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001052651 – 579Fatty-acid amide hydrolase 1Add BLAST579

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei241PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO08914.
MaxQBiO08914.
PaxDbiO08914.
PeptideAtlasiO08914.
PRIDEiO08914.

PTM databases

iPTMnetiO08914.
PhosphoSitePlusiO08914.

Expressioni

Gene expression databases

BgeeiENSMUSG00000034171.
CleanExiMM_FAAH.
GenevisibleiO08914. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

MINTiMINT-1838003.
STRINGi10090.ENSMUSP00000041543.

Chemistry databases

BindingDBiO08914.

Structurei

3D structure databases

ProteinModelPortaliO08914.
SMRiO08914.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni238 – 241Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the amidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1212. Eukaryota.
COG0154. LUCA.
GeneTreeiENSGT00550000074673.
HOGENOMiHOG000016500.
HOVERGENiHBG005632.
InParanoidiO08914.
KOiK15528.
OMAiNKETNCV.
OrthoDBiEOG091G05JU.
PhylomeDBiO08914.
TreeFamiTF314455.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR030560. FAAH.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PTHR11895:SF91. PTHR11895:SF91. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08914-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLSEVWTAL SGLSGVCLAC SLLSAAVVLR WTRSQTARGA VTRARQKQRA
60 70 80 90 100
GLETMDKAVQ RFRLQNPDLD SEALLALPLL QLVQKLQSGE LSPEAVLFTY
110 120 130 140 150
LGKAWEVNKG TNCVTSYLTD CETQLSQAPR QGLLYGVPVS LKECFSYKGH
160 170 180 190 200
ASTLGLSLNE GVTSESDCVV VQVLKLQGAV PFVHTNVPQS MLSYDCSNPL
210 220 230 240 250
FGQTMNPWKP SKSPGGSSGG EGALIGSGGS PLGLGTDIGG SIRFPSAFCG
260 270 280 290 300
ICGLKPTGNR LSKSGLKSCV YGQTAVQLSV GPMARDVDSL ALCMKALLCE
310 320 330 340 350
DLFRLDSTIP PLPFREEIYR SSRPLRVGYY ETDNYTMPTP AMRRAVMETK
360 370 380 390 400
QSLEAAGHTL VPFLPNNIPY ALEVLSAGGL FSDGGCSFLQ NFKGDFVDPC
410 420 430 440 450
LGDLVLVLKL PRWFKKLLSF LLKPLFPRLA AFLNSMCPRS AEKLWELQHE
460 470 480 490 500
IEMYRQSVIA QWKAMNLDVV LTPMLGPALD LNTPGRATGA ISYTVLYNCL
510 520 530 540 550
DFPAGVVPVT TVTAEDDAQM EHYKGYFGDM WDNILKKGMK KGIGLPVAVQ
560 570
CVALPWQEEL CLRFMREVER LMTPEKRPS
Length:579
Mass (Da):63,221
Last modified:July 1, 1997 - v1
Checksum:i11B2DF6CBC110015
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72E → Q in AAH06863 (PubMed:15489334).Curated1
Sequence conflicti230S → P in AK004985 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82536 mRNA. Translation: AAB58506.1.
AF098009
, AF097997, AF097998, AF097999, AF098000, AF098001, AF098002, AF098003, AF098004, AF098005, AF098006, AF098007, AF098008 Genomic DNA. Translation: AAD11788.1.
AK004985 mRNA. No translation available.
BC006863 mRNA. Translation: AAH06863.1.
BC052321 mRNA. Translation: AAH52321.1.
CCDSiCCDS18501.1.
RefSeqiNP_034303.3. NM_010173.4.
UniGeneiMm.256025.

Genome annotation databases

EnsembliENSMUST00000049095; ENSMUSP00000041543; ENSMUSG00000034171.
GeneIDi14073.
KEGGimmu:14073.
UCSCiuc008ufs.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82536 mRNA. Translation: AAB58506.1.
AF098009
, AF097997, AF097998, AF097999, AF098000, AF098001, AF098002, AF098003, AF098004, AF098005, AF098006, AF098007, AF098008 Genomic DNA. Translation: AAD11788.1.
AK004985 mRNA. No translation available.
BC006863 mRNA. Translation: AAH06863.1.
BC052321 mRNA. Translation: AAH52321.1.
CCDSiCCDS18501.1.
RefSeqiNP_034303.3. NM_010173.4.
UniGeneiMm.256025.

3D structure databases

ProteinModelPortaliO08914.
SMRiO08914.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1838003.
STRINGi10090.ENSMUSP00000041543.

Chemistry databases

BindingDBiO08914.
ChEMBLiCHEMBL3455.

PTM databases

iPTMnetiO08914.
PhosphoSitePlusiO08914.

Proteomic databases

EPDiO08914.
MaxQBiO08914.
PaxDbiO08914.
PeptideAtlasiO08914.
PRIDEiO08914.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049095; ENSMUSP00000041543; ENSMUSG00000034171.
GeneIDi14073.
KEGGimmu:14073.
UCSCiuc008ufs.2. mouse.

Organism-specific databases

CTDi2166.
MGIiMGI:109609. Faah.

Phylogenomic databases

eggNOGiKOG1212. Eukaryota.
COG0154. LUCA.
GeneTreeiENSGT00550000074673.
HOGENOMiHOG000016500.
HOVERGENiHBG005632.
InParanoidiO08914.
KOiK15528.
OMAiNKETNCV.
OrthoDBiEOG091G05JU.
PhylomeDBiO08914.
TreeFamiTF314455.

Enzyme and pathway databases

BRENDAi3.5.1.4. 3474.
3.5.1.99. 3474.
ReactomeiR-MMU-2142753. Arachidonic acid metabolism.

Miscellaneous databases

PROiO08914.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034171.
CleanExiMM_FAAH.
GenevisibleiO08914. MM.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR030560. FAAH.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PTHR11895:SF91. PTHR11895:SF91. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFAAH1_MOUSE
AccessioniPrimary (citable) accession number: O08914
Secondary accession number(s): Q922S0, Q9DBF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.