Phosphatidylinositol 3-kinase regulatory subunit beta

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O08908 (P85B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 119. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 3-kinase regulatory subunit beta
Short name=PI3-kinase regulatory subunit beta
Short name=PI3K regulatory subunit beta
Short name=PtdIns-3-kinase regulatory subunit beta

Alternative name(s):
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
Short name=PI3-kinase subunit p85-beta
Short name=PtdIns-3-kinase regulatory subunit p85-beta

Gene names

Name:

Pik3r2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	722 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
Subunit structure	Heterodimer of a regulatory subunit PIK3R2 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Interacts with FLT1 (tyrosine-phosphorylated) and FLT4 (tyrosine-phosphorylated) By similarity. Interacts with NYAP1, NYAP2 and MYO16. Ref.6
Post-translational modification	Phosphorylated in response to signaling from activated receptor-type protein kinases. Dephosphorylated by PTPRJ By similarity.
Sequence similarities	Belongs to the PI3K p85 subunit family. Contains 1 Rho-GAP domain. Contains 2 SH2 domains. Contains 1 SH3 domain.

Ontologies

Keywords
Domain	Repeat SH2 domain SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	insulin receptor signaling pathway Non-traceable author statement PubMed 14504291. Source: UniProtKB phosphatidylinositol-3-phosphate biosynthetic process Inferred from mutant phenotype PubMed 14504291. Source: GOC regulation of phosphatidylinositol 3-kinase activity Inferred from electronic annotation. Source: GOC
Cellular_component	phosphatidylinositol 3-kinase complex Non-traceable author statement PubMed 14504291. Source: UniProtKB
Molecular_function	phosphatidylinositol 3-kinase regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Brd7	O88665	4	EBI-643570,EBI-643930
Pdk2	Q9JK42	3	EBI-643570,EBI-643530

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 722

722

Phosphatidylinositol 3-kinase regulatory subunit beta

PRO_0000080764

Regions

Domain

4 – 80

SH3

Domain

112 – 293

182

Rho-GAP

Domain

324 – 419

SH2 1

Domain

616 – 710

SH2 2

Amino acid modifications

Modified residue

458

Phosphotyrosine Ref.4 Ref.5

Modified residue

599

Phosphotyrosine By similarity

Experimental info

Sequence conflict

433

I → V in CAA73903. Ref.1

Sequence conflict

433

I → V in AAH06796. Ref.3

Secondary structure

722

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

O08908 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: A999FAF9011455FE
Show »

FASTA

722

81,266

        10         20         30         40         50         60 
MAGAEGFQYR AVYPFRRERP EDLELLPGDL LVVSRVALQA LGVADGGERC PHNVGWMPGF 

        70         80         90        100        110        120 
NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP LDGSSESGHI LPDLAEQFSP 

       130        140        150        160        170        180 
PDPAPPILVK LVEAIEQAEL DSECYSKPEL PATRTDWSLS DLEQWDRTAL YDAVKGFLLA 

       190        200        210        220        230        240 
LPAAVVTPEA AAEAYRALRE VAGPVGLVLE PPTLPLHQAL TLRFLLQHLG RVARRAPSPD 

       250        260        270        280        290        300 
TAVHALASAF GPLLLRIPPS GGEGDGSEPV PDFPVLLLER LVQEHVEEQD AAPPALPPKP 

       310        320        330        340        350        360 
SKAKPAPTAL ANGGSPPSLQ DAEWYWGDIS REEVNERLRD TPDGTFLVRD ASSKIQGEYT 

       370        380        390        400        410        420 
LTLRKGGNNK LIKVFHRDGH YGFSEPLTFC SVVELISHYR HESLAQYNAK LDTRLLYPVS 

       430        440        450        460        470        480 
KYQQDQVVKE DSIEAVGAQL KVYHQQYQDK SREYDQLYEE YTRTSQELQM KRTAIEAFNE 

       490        500        510        520        530        540 
TIKIFEEQGQ TQEKCSKEYL ERFRREGNEK EMQRILLNSE RLKSRIAEIH ESRTKLEQDL 

       550        560        570        580        590        600 
RAQASDNREI DKRMNSLKPD LMQLRKIRDQ YLVWLTQKGA RQRKINEWLG IKNETEDQYS 

       610        620        630        640        650        660 
LMEDEDALPH HEERTWYVGK INRTQAEEML SGKRDGTFLI RESSQRGCYA CSVVVDGDTK 

       670        680        690        700        710        720 
HCVIYRTATG FGFAEPYNLY GSLKELVLHY QHASLVQHND ALTVTLAHPV RAPGPGPPSA 


AR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An oncogenic fusion product of the phosphatidylinositol 3-kinase p85beta subunit and HUMORF8, a putative deubiquitinating enzyme." Janssen J.W.G., Schleithhoff L., Bartram C.R., Schulz A.S. Oncogene 16:1767-1772(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: NIH Swiss.
[2]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6 and FVB/N. Tissue: Brain and Mammary gland.
[4]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-458, MASS SPECTROMETRY. Tissue: Mast cell.
[5]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-458, MASS SPECTROMETRY. Tissue: Brain.
[6]	"NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons." Yokoyama K., Tezuka T., Kotani M., Nakazawa T., Hoshina N., Shimoda Y., Kakuta S., Sudo K., Watanabe K., Iwakura Y., Yamamoto T. EMBO J. 30:4739-4754(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NYAP1; NYAP2 AND MYO16.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y13569 mRNA. Translation: CAA73903.1.
CH466569 Genomic DNA. Translation: EDL28873.1.
BC006796 mRNA. Translation: AAH06796.1.
BC085501 mRNA. Translation: AAH85501.1.

IPI

IPI00117159.

RefSeq

NP_032867.2. NM_008841.2.

UniGene

Mm.12945.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2Y3A	X-ray	3.30	B	423-722	[»]

ProteinModelPortal

O08908.

SMR

O08908. Positions 5-82, 111-288, 318-714.

ModBase

Search...

Protein-protein interaction databases

IntAct

O08908. 10 interactions.

PTM databases

PhosphoSite

O08908.

Proteomic databases

PaxDb

O08908.

PRIDE

O08908.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000034296; ENSMUSP00000034296; ENSMUSG00000031834.

GeneID

18709.

KEGG

mmu:18709.

Organism-specific databases

CTD

5296.

MGI

MGI:1098772. Pik3r2.

Phylogenomic databases

eggNOG

NOG263689.

GeneTree

ENSGT00390000010431.

HOGENOM

HOG000008438.

HOVERGEN

HBG082100.

InParanoid

Q5U3K7.

K02649.

OMA

SDVDQWD.

OrthoDB

EOG49GKG4.

Enzyme and pathway databases

Reactome

REACT_107772. Immune System.

Gene expression databases

ArrayExpress

O08908.

Bgee

O08908.

Genevestigator

O08908.

GermOnline

ENSMUSG00000031834. Mus musculus.

Family and domain databases

Gene3D

1.10.555.10. 1 hit.
3.30.505.10. 2 hits.

InterPro

IPR001720. PI3kinase_P85.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]

PANTHER

PTHR10155. PTHR10155. 1 hit.

Pfam

PF00620. RhoGAP. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view]

PRINTS

PR00678. PI3KINASEP85.
PR00401. SH2DOMAIN.

SMART

SM00324. RhoGAP. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF48350. Rho_GAP. 1 hit.
SSF50044. SH3. 1 hit.

PROSITE

PS50238. RHOGAP. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

294785.

SOURCE

Search...

Entry information

Entry name

P85B_MOUSE

Accession

Primary (citable) accession number: O08908
Secondary accession number(s): Q5U3K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents