SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O08908

- P85B_MOUSE

UniProt

O08908 - P85B_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Phosphatidylinositol 3-kinase regulatory subunit beta

Gene
Pik3r2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.

GO - Molecular functioni

  1. phosphatidylinositol 3-kinase regulator activity Source: InterPro
  2. protein binding Source: UniProtKB

GO - Biological processi

  1. insulin receptor signaling pathway Source: UniProtKB
  2. phosphatidylinositol-3-phosphate biosynthetic process Source: GOC
  3. regulation of phosphorylation Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_188185. DAP12 signaling.
REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_198701. Interleukin-7 signaling.
REACT_198973. Synthesis of PIPs at the plasma membrane.
REACT_205300. PI3K/AKT activation.
REACT_210090. Rho GTPase cycle.
REACT_210240. Role of phospholipids in phagocytosis.
REACT_211860. Tie2 Signaling.
REACT_213364. Role of LAT2/NTAL/LAB on calcium mobilization.
REACT_225145. Downstream TCR signaling.
REACT_226341. PIP3 activates AKT signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase regulatory subunit beta
Short name:
PI3-kinase regulatory subunit beta
Short name:
PI3K regulatory subunit beta
Short name:
PtdIns-3-kinase regulatory subunit beta
Alternative name(s):
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
Short name:
PI3-kinase subunit p85-beta
Short name:
PtdIns-3-kinase regulatory subunit p85-beta
Gene namesi
Name:Pik3r2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1098772. Pik3r2.

Subcellular locationi

GO - Cellular componenti

  1. phosphatidylinositol 3-kinase complex Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 722722Phosphatidylinositol 3-kinase regulatory subunit betaPRO_0000080764Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei458 – 4581Phosphotyrosine3 Publications
Modified residuei599 – 5991Phosphotyrosine By similarity

Post-translational modificationi

Phosphorylated in response to signaling from activated receptor-type protein kinases. Dephosphorylated by PTPRJ By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO08908.
PRIDEiO08908.

PTM databases

PhosphoSiteiO08908.

Expressioni

Gene expression databases

ArrayExpressiO08908.
BgeeiO08908.
GenevestigatoriO08908.

Interactioni

Subunit structurei

Heterodimer of a regulatory subunit PIK3R2 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Interacts with FLT1 (tyrosine-phosphorylated) and FLT4 (tyrosine-phosphorylated) By similarity. Interacts with NYAP1, NYAP2 and MYO16.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Brd7O886654EBI-643570,EBI-643930
Pdk2Q9JK423EBI-643570,EBI-643530

Protein-protein interaction databases

BioGridi202163. 4 interactions.
IntActiO08908. 10 interactions.
MINTiMINT-1723872.

Structurei

Secondary structure

1
722
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi448 – 4514
Turni452 – 4543
Helixi455 – 49642
Turni508 – 5103
Helixi511 – 55747
Helixi559 – 56810
Helixi609 – 6113
Turni614 – 6163
Helixi623 – 6308
Beta strandi637 – 6404
Beta strandi649 – 6546
Beta strandi656 – 66712
Beta strandi670 – 6745
Helixi683 – 6886
Beta strandi691 – 6933
Turni696 – 6983

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y3AX-ray3.30B423-722[»]
ProteinModelPortaliO08908.
SMRiO08908. Positions 5-82, 111-288, 318-714.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 8077SH3Add
BLAST
Domaini112 – 293182Rho-GAPAdd
BLAST
Domaini324 – 41996SH2 1Add
BLAST
Domaini616 – 71095SH2 2Add
BLAST

Sequence similaritiesi

Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiNOG263689.
GeneTreeiENSGT00390000010431.
HOGENOMiHOG000008438.
HOVERGENiHBG082100.
InParanoidiQ5U3K7.
KOiK02649.
OMAiHCVIYKT.
OrthoDBiEOG7BP831.
TreeFamiTF102033.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
3.30.505.10. 2 hits.
InterProiIPR001720. PI3kinase_P85.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10155. PTHR10155. 1 hit.
PfamiPF00620. RhoGAP. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view]
PRINTSiPR00678. PI3KINASEP85.
PR00401. SH2DOMAIN.
SMARTiSM00324. RhoGAP. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 2 hits.
PROSITEiPS50238. RHOGAP. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08908-1 [UniParc]FASTAAdd to Basket

« Hide

MAGAEGFQYR AVYPFRRERP EDLELLPGDL LVVSRVALQA LGVADGGERC    50
PHNVGWMPGF NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP 100
LDGSSESGHI LPDLAEQFSP PDPAPPILVK LVEAIEQAEL DSECYSKPEL 150
PATRTDWSLS DLEQWDRTAL YDAVKGFLLA LPAAVVTPEA AAEAYRALRE 200
VAGPVGLVLE PPTLPLHQAL TLRFLLQHLG RVARRAPSPD TAVHALASAF 250
GPLLLRIPPS GGEGDGSEPV PDFPVLLLER LVQEHVEEQD AAPPALPPKP 300
SKAKPAPTAL ANGGSPPSLQ DAEWYWGDIS REEVNERLRD TPDGTFLVRD 350
ASSKIQGEYT LTLRKGGNNK LIKVFHRDGH YGFSEPLTFC SVVELISHYR 400
HESLAQYNAK LDTRLLYPVS KYQQDQVVKE DSIEAVGAQL KVYHQQYQDK 450
SREYDQLYEE YTRTSQELQM KRTAIEAFNE TIKIFEEQGQ TQEKCSKEYL 500
ERFRREGNEK EMQRILLNSE RLKSRIAEIH ESRTKLEQDL RAQASDNREI 550
DKRMNSLKPD LMQLRKIRDQ YLVWLTQKGA RQRKINEWLG IKNETEDQYS 600
LMEDEDALPH HEERTWYVGK INRTQAEEML SGKRDGTFLI RESSQRGCYA 650
CSVVVDGDTK HCVIYRTATG FGFAEPYNLY GSLKELVLHY QHASLVQHND 700
ALTVTLAHPV RAPGPGPPSA AR 722
Length:722
Mass (Da):81,266
Last modified:July 27, 2011 - v2
Checksum:iA999FAF9011455FE
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti433 – 4331I → V in CAA73903. 1 Publication
Sequence conflicti433 – 4331I → V in AAH06796. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13569 mRNA. Translation: CAA73903.1.
CH466569 Genomic DNA. Translation: EDL28873.1.
BC006796 mRNA. Translation: AAH06796.1.
BC085501 mRNA. Translation: AAH85501.1.
CCDSiCCDS22381.1.
RefSeqiNP_032867.2. NM_008841.2.
UniGeneiMm.12945.

Genome annotation databases

EnsembliENSMUST00000034296; ENSMUSP00000034296; ENSMUSG00000031834.
GeneIDi18709.
KEGGimmu:18709.
UCSCiuc009mbn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13569 mRNA. Translation: CAA73903.1 .
CH466569 Genomic DNA. Translation: EDL28873.1 .
BC006796 mRNA. Translation: AAH06796.1 .
BC085501 mRNA. Translation: AAH85501.1 .
CCDSi CCDS22381.1.
RefSeqi NP_032867.2. NM_008841.2.
UniGenei Mm.12945.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2Y3A X-ray 3.30 B 423-722 [» ]
ProteinModelPortali O08908.
SMRi O08908. Positions 5-82, 111-288, 318-714.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202163. 4 interactions.
IntActi O08908. 10 interactions.
MINTi MINT-1723872.

PTM databases

PhosphoSitei O08908.

Proteomic databases

PaxDbi O08908.
PRIDEi O08908.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034296 ; ENSMUSP00000034296 ; ENSMUSG00000031834 .
GeneIDi 18709.
KEGGi mmu:18709.
UCSCi uc009mbn.2. mouse.

Organism-specific databases

CTDi 5296.
MGIi MGI:1098772. Pik3r2.

Phylogenomic databases

eggNOGi NOG263689.
GeneTreei ENSGT00390000010431.
HOGENOMi HOG000008438.
HOVERGENi HBG082100.
InParanoidi Q5U3K7.
KOi K02649.
OMAi HCVIYKT.
OrthoDBi EOG7BP831.
TreeFami TF102033.

Enzyme and pathway databases

Reactomei REACT_188185. DAP12 signaling.
REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_198701. Interleukin-7 signaling.
REACT_198973. Synthesis of PIPs at the plasma membrane.
REACT_205300. PI3K/AKT activation.
REACT_210090. Rho GTPase cycle.
REACT_210240. Role of phospholipids in phagocytosis.
REACT_211860. Tie2 Signaling.
REACT_213364. Role of LAT2/NTAL/LAB on calcium mobilization.
REACT_225145. Downstream TCR signaling.
REACT_226341. PIP3 activates AKT signaling.

Miscellaneous databases

NextBioi 294785.
PROi O08908.
SOURCEi Search...

Gene expression databases

ArrayExpressi O08908.
Bgeei O08908.
Genevestigatori O08908.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
3.30.505.10. 2 hits.
InterProi IPR001720. PI3kinase_P85.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view ]
PANTHERi PTHR10155. PTHR10155. 1 hit.
Pfami PF00620. RhoGAP. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view ]
PRINTSi PR00678. PI3KINASEP85.
PR00401. SH2DOMAIN.
SMARTi SM00324. RhoGAP. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 2 hits.
PROSITEi PS50238. RHOGAP. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An oncogenic fusion product of the phosphatidylinositol 3-kinase p85beta subunit and HUMORF8, a putative deubiquitinating enzyme."
    Janssen J.W.G., Schleithhoff L., Bartram C.R., Schulz A.S.
    Oncogene 16:1767-1772(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NIH Swiss.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain and Mammary gland.
  4. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  6. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  8. "NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons."
    Yokoyama K., Tezuka T., Kotani M., Nakazawa T., Hoshina N., Shimoda Y., Kakuta S., Sudo K., Watanabe K., Iwakura Y., Yamamoto T.
    EMBO J. 30:4739-4754(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NYAP1; NYAP2 AND MYO16.

Entry informationi

Entry nameiP85B_MOUSE
AccessioniPrimary (citable) accession number: O08908
Secondary accession number(s): Q5U3K7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi