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O08908 (P85B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol 3-kinase regulatory subunit beta

Short name=PI3-kinase regulatory subunit beta
Short name=PI3K regulatory subunit beta
Short name=PtdIns-3-kinase regulatory subunit beta
Alternative name(s):
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
Short name=PI3-kinase subunit p85-beta
Short name=PtdIns-3-kinase regulatory subunit p85-beta
Gene names
Name:Pik3r2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length722 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.

Subunit structure

Heterodimer of a regulatory subunit PIK3R2 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Interacts with FLT1 (tyrosine-phosphorylated) and FLT4 (tyrosine-phosphorylated) By similarity. Interacts with NYAP1, NYAP2 and MYO16. Ref.8

Post-translational modification

Phosphorylated in response to signaling from activated receptor-type protein kinases. Dephosphorylated by PTPRJ By similarity.

Sequence similarities

Belongs to the PI3K p85 subunit family.

Contains 1 Rho-GAP domain.

Contains 2 SH2 domains.

Contains 1 SH3 domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 722722Phosphatidylinositol 3-kinase regulatory subunit beta
PRO_0000080764

Regions

Domain4 – 8077SH3
Domain112 – 293182Rho-GAP
Domain324 – 41996SH2 1
Domain616 – 71095SH2 2

Amino acid modifications

Modified residue4581Phosphotyrosine Ref.4 Ref.6 Ref.7
Modified residue5991Phosphotyrosine By similarity

Experimental info

Sequence conflict4331I → V in CAA73903. Ref.1
Sequence conflict4331I → V in AAH06796. Ref.3

Secondary structure

.............................. 722
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O08908 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: A999FAF9011455FE

FASTA72281,266
        10         20         30         40         50         60 
MAGAEGFQYR AVYPFRRERP EDLELLPGDL LVVSRVALQA LGVADGGERC PHNVGWMPGF 

        70         80         90        100        110        120 
NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP LDGSSESGHI LPDLAEQFSP 

       130        140        150        160        170        180 
PDPAPPILVK LVEAIEQAEL DSECYSKPEL PATRTDWSLS DLEQWDRTAL YDAVKGFLLA 

       190        200        210        220        230        240 
LPAAVVTPEA AAEAYRALRE VAGPVGLVLE PPTLPLHQAL TLRFLLQHLG RVARRAPSPD 

       250        260        270        280        290        300 
TAVHALASAF GPLLLRIPPS GGEGDGSEPV PDFPVLLLER LVQEHVEEQD AAPPALPPKP 

       310        320        330        340        350        360 
SKAKPAPTAL ANGGSPPSLQ DAEWYWGDIS REEVNERLRD TPDGTFLVRD ASSKIQGEYT 

       370        380        390        400        410        420 
LTLRKGGNNK LIKVFHRDGH YGFSEPLTFC SVVELISHYR HESLAQYNAK LDTRLLYPVS 

       430        440        450        460        470        480 
KYQQDQVVKE DSIEAVGAQL KVYHQQYQDK SREYDQLYEE YTRTSQELQM KRTAIEAFNE 

       490        500        510        520        530        540 
TIKIFEEQGQ TQEKCSKEYL ERFRREGNEK EMQRILLNSE RLKSRIAEIH ESRTKLEQDL 

       550        560        570        580        590        600 
RAQASDNREI DKRMNSLKPD LMQLRKIRDQ YLVWLTQKGA RQRKINEWLG IKNETEDQYS 

       610        620        630        640        650        660 
LMEDEDALPH HEERTWYVGK INRTQAEEML SGKRDGTFLI RESSQRGCYA CSVVVDGDTK 

       670        680        690        700        710        720 
HCVIYRTATG FGFAEPYNLY GSLKELVLHY QHASLVQHND ALTVTLAHPV RAPGPGPPSA 


AR 

« Hide

References

« Hide 'large scale' references
[1]"An oncogenic fusion product of the phosphatidylinositol 3-kinase p85beta subunit and HUMORF8, a putative deubiquitinating enzyme."
Janssen J.W.G., Schleithhoff L., Bartram C.R., Schulz A.S.
Oncogene 16:1767-1772(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: NIH Swiss.
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Mammary gland.
[4]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[6]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Mast cell.
[7]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[8]"NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons."
Yokoyama K., Tezuka T., Kotani M., Nakazawa T., Hoshina N., Shimoda Y., Kakuta S., Sudo K., Watanabe K., Iwakura Y., Yamamoto T.
EMBO J. 30:4739-4754(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NYAP1; NYAP2 AND MYO16.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y13569 mRNA. Translation: CAA73903.1.
CH466569 Genomic DNA. Translation: EDL28873.1.
BC006796 mRNA. Translation: AAH06796.1.
BC085501 mRNA. Translation: AAH85501.1.
CCDSCCDS22381.1.
RefSeqNP_032867.2. NM_008841.2.
UniGeneMm.12945.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y3AX-ray3.30B423-722[»]
ProteinModelPortalO08908.
SMRO08908. Positions 5-82, 111-288, 318-714.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202163. 4 interactions.
IntActO08908. 10 interactions.
MINTMINT-1723872.

PTM databases

PhosphoSiteO08908.

Proteomic databases

PaxDbO08908.
PRIDEO08908.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034296; ENSMUSP00000034296; ENSMUSG00000031834.
GeneID18709.
KEGGmmu:18709.
UCSCuc009mbn.2. mouse.

Organism-specific databases

CTD5296.
MGIMGI:1098772. Pik3r2.

Phylogenomic databases

eggNOGNOG263689.
GeneTreeENSGT00390000010431.
HOGENOMHOG000008438.
HOVERGENHBG082100.
InParanoidQ5U3K7.
KOK02649.
OMAHCVIYKT.
OrthoDBEOG7BP831.
TreeFamTF102033.

Gene expression databases

ArrayExpressO08908.
BgeeO08908.
GenevestigatorO08908.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
3.30.505.10. 2 hits.
InterProIPR001720. PI3kinase_P85.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERPTHR10155. PTHR10155. 1 hit.
PfamPF00620. RhoGAP. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view]
PRINTSPR00678. PI3KINASEP85.
PR00401. SH2DOMAIN.
SMARTSM00324. RhoGAP. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 2 hits.
PROSITEPS50238. RHOGAP. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio294785.
PROO08908.
SOURCESearch...

Entry information

Entry nameP85B_MOUSE
AccessionPrimary (citable) accession number: O08908
Secondary accession number(s): Q5U3K7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot