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O08908

- P85B_MOUSE

UniProt

O08908 - P85B_MOUSE

Protein

Phosphatidylinositol 3-kinase regulatory subunit beta

Gene

Pik3r2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.

    GO - Molecular functioni

    1. phosphatidylinositol 3-kinase regulator activity Source: InterPro
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. insulin receptor signaling pathway Source: UniProtKB
    2. phosphatidylinositol-3-phosphate biosynthetic process Source: GOC
    3. regulation of phosphorylation Source: UniProtKB

    Enzyme and pathway databases

    ReactomeiREACT_188185. DAP12 signaling.
    REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
    REACT_198701. Interleukin-7 signaling.
    REACT_198973. Synthesis of PIPs at the plasma membrane.
    REACT_205300. PI3K/AKT activation.
    REACT_210090. Rho GTPase cycle.
    REACT_210240. Role of phospholipids in phagocytosis.
    REACT_211860. Tie2 Signaling.
    REACT_213364. Role of LAT2/NTAL/LAB on calcium mobilization.
    REACT_225145. Downstream TCR signaling.
    REACT_226341. PIP3 activates AKT signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 3-kinase regulatory subunit beta
    Short name:
    PI3-kinase regulatory subunit beta
    Short name:
    PI3K regulatory subunit beta
    Short name:
    PtdIns-3-kinase regulatory subunit beta
    Alternative name(s):
    Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
    Short name:
    PI3-kinase subunit p85-beta
    Short name:
    PtdIns-3-kinase regulatory subunit p85-beta
    Gene namesi
    Name:Pik3r2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1098772. Pik3r2.

    Subcellular locationi

    GO - Cellular componenti

    1. phosphatidylinositol 3-kinase complex Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 722722Phosphatidylinositol 3-kinase regulatory subunit betaPRO_0000080764Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei458 – 4581Phosphotyrosine3 Publications
    Modified residuei599 – 5991PhosphotyrosineBy similarity

    Post-translational modificationi

    Phosphorylated in response to signaling from activated receptor-type protein kinases. Dephosphorylated by PTPRJ By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO08908.
    PRIDEiO08908.

    PTM databases

    PhosphoSiteiO08908.

    Expressioni

    Gene expression databases

    ArrayExpressiO08908.
    BgeeiO08908.
    GenevestigatoriO08908.

    Interactioni

    Subunit structurei

    Heterodimer of a regulatory subunit PIK3R2 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Interacts with FLT1 (tyrosine-phosphorylated) and FLT4 (tyrosine-phosphorylated) By similarity. Interacts with NYAP1, NYAP2 and MYO16.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Brd7O886654EBI-643570,EBI-643930
    Pdk2Q9JK423EBI-643570,EBI-643530

    Protein-protein interaction databases

    BioGridi202163. 4 interactions.
    IntActiO08908. 10 interactions.
    MINTiMINT-1723872.

    Structurei

    Secondary structure

    1
    722
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi448 – 4514
    Turni452 – 4543
    Helixi455 – 49642
    Turni508 – 5103
    Helixi511 – 55747
    Helixi559 – 56810
    Helixi609 – 6113
    Turni614 – 6163
    Helixi623 – 6308
    Beta strandi637 – 6404
    Beta strandi649 – 6546
    Beta strandi656 – 66712
    Beta strandi670 – 6745
    Helixi683 – 6886
    Beta strandi691 – 6933
    Turni696 – 6983

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2Y3AX-ray3.30B423-722[»]
    ProteinModelPortaliO08908.
    SMRiO08908. Positions 5-82, 111-288, 318-714.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 8077SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini112 – 293182Rho-GAPPROSITE-ProRule annotationAdd
    BLAST
    Domaini324 – 41996SH2 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini616 – 71095SH2 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PI3K p85 subunit family.Curated
    Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
    Contains 2 SH2 domains.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiNOG263689.
    GeneTreeiENSGT00390000010431.
    HOGENOMiHOG000008438.
    HOVERGENiHBG082100.
    InParanoidiQ5U3K7.
    KOiK02649.
    OMAiHCVIYKT.
    OrthoDBiEOG7BP831.
    TreeFamiTF102033.

    Family and domain databases

    Gene3Di1.10.555.10. 1 hit.
    3.30.505.10. 2 hits.
    InterProiIPR001720. PI3kinase_P85.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    [Graphical view]
    PANTHERiPTHR10155. PTHR10155. 1 hit.
    PfamiPF00620. RhoGAP. 1 hit.
    PF00017. SH2. 2 hits.
    [Graphical view]
    PRINTSiPR00678. PI3KINASEP85.
    PR00401. SH2DOMAIN.
    SMARTiSM00324. RhoGAP. 1 hit.
    SM00252. SH2. 2 hits.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    SSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 2 hits.
    PROSITEiPS50238. RHOGAP. 1 hit.
    PS50001. SH2. 2 hits.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O08908-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGAEGFQYR AVYPFRRERP EDLELLPGDL LVVSRVALQA LGVADGGERC    50
    PHNVGWMPGF NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP 100
    LDGSSESGHI LPDLAEQFSP PDPAPPILVK LVEAIEQAEL DSECYSKPEL 150
    PATRTDWSLS DLEQWDRTAL YDAVKGFLLA LPAAVVTPEA AAEAYRALRE 200
    VAGPVGLVLE PPTLPLHQAL TLRFLLQHLG RVARRAPSPD TAVHALASAF 250
    GPLLLRIPPS GGEGDGSEPV PDFPVLLLER LVQEHVEEQD AAPPALPPKP 300
    SKAKPAPTAL ANGGSPPSLQ DAEWYWGDIS REEVNERLRD TPDGTFLVRD 350
    ASSKIQGEYT LTLRKGGNNK LIKVFHRDGH YGFSEPLTFC SVVELISHYR 400
    HESLAQYNAK LDTRLLYPVS KYQQDQVVKE DSIEAVGAQL KVYHQQYQDK 450
    SREYDQLYEE YTRTSQELQM KRTAIEAFNE TIKIFEEQGQ TQEKCSKEYL 500
    ERFRREGNEK EMQRILLNSE RLKSRIAEIH ESRTKLEQDL RAQASDNREI 550
    DKRMNSLKPD LMQLRKIRDQ YLVWLTQKGA RQRKINEWLG IKNETEDQYS 600
    LMEDEDALPH HEERTWYVGK INRTQAEEML SGKRDGTFLI RESSQRGCYA 650
    CSVVVDGDTK HCVIYRTATG FGFAEPYNLY GSLKELVLHY QHASLVQHND 700
    ALTVTLAHPV RAPGPGPPSA AR 722
    Length:722
    Mass (Da):81,266
    Last modified:July 27, 2011 - v2
    Checksum:iA999FAF9011455FE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti433 – 4331I → V in CAA73903. (PubMed:9582025)Curated
    Sequence conflicti433 – 4331I → V in AAH06796. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13569 mRNA. Translation: CAA73903.1.
    CH466569 Genomic DNA. Translation: EDL28873.1.
    BC006796 mRNA. Translation: AAH06796.1.
    BC085501 mRNA. Translation: AAH85501.1.
    CCDSiCCDS22381.1.
    RefSeqiNP_032867.2. NM_008841.2.
    UniGeneiMm.12945.

    Genome annotation databases

    EnsembliENSMUST00000034296; ENSMUSP00000034296; ENSMUSG00000031834.
    GeneIDi18709.
    KEGGimmu:18709.
    UCSCiuc009mbn.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13569 mRNA. Translation: CAA73903.1 .
    CH466569 Genomic DNA. Translation: EDL28873.1 .
    BC006796 mRNA. Translation: AAH06796.1 .
    BC085501 mRNA. Translation: AAH85501.1 .
    CCDSi CCDS22381.1.
    RefSeqi NP_032867.2. NM_008841.2.
    UniGenei Mm.12945.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2Y3A X-ray 3.30 B 423-722 [» ]
    ProteinModelPortali O08908.
    SMRi O08908. Positions 5-82, 111-288, 318-714.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202163. 4 interactions.
    IntActi O08908. 10 interactions.
    MINTi MINT-1723872.

    PTM databases

    PhosphoSitei O08908.

    Proteomic databases

    PaxDbi O08908.
    PRIDEi O08908.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034296 ; ENSMUSP00000034296 ; ENSMUSG00000031834 .
    GeneIDi 18709.
    KEGGi mmu:18709.
    UCSCi uc009mbn.2. mouse.

    Organism-specific databases

    CTDi 5296.
    MGIi MGI:1098772. Pik3r2.

    Phylogenomic databases

    eggNOGi NOG263689.
    GeneTreei ENSGT00390000010431.
    HOGENOMi HOG000008438.
    HOVERGENi HBG082100.
    InParanoidi Q5U3K7.
    KOi K02649.
    OMAi HCVIYKT.
    OrthoDBi EOG7BP831.
    TreeFami TF102033.

    Enzyme and pathway databases

    Reactomei REACT_188185. DAP12 signaling.
    REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
    REACT_198701. Interleukin-7 signaling.
    REACT_198973. Synthesis of PIPs at the plasma membrane.
    REACT_205300. PI3K/AKT activation.
    REACT_210090. Rho GTPase cycle.
    REACT_210240. Role of phospholipids in phagocytosis.
    REACT_211860. Tie2 Signaling.
    REACT_213364. Role of LAT2/NTAL/LAB on calcium mobilization.
    REACT_225145. Downstream TCR signaling.
    REACT_226341. PIP3 activates AKT signaling.

    Miscellaneous databases

    NextBioi 294785.
    PROi O08908.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O08908.
    Bgeei O08908.
    Genevestigatori O08908.

    Family and domain databases

    Gene3Di 1.10.555.10. 1 hit.
    3.30.505.10. 2 hits.
    InterProi IPR001720. PI3kinase_P85.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    [Graphical view ]
    PANTHERi PTHR10155. PTHR10155. 1 hit.
    Pfami PF00620. RhoGAP. 1 hit.
    PF00017. SH2. 2 hits.
    [Graphical view ]
    PRINTSi PR00678. PI3KINASEP85.
    PR00401. SH2DOMAIN.
    SMARTi SM00324. RhoGAP. 1 hit.
    SM00252. SH2. 2 hits.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    SSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 2 hits.
    PROSITEi PS50238. RHOGAP. 1 hit.
    PS50001. SH2. 2 hits.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An oncogenic fusion product of the phosphatidylinositol 3-kinase p85beta subunit and HUMORF8, a putative deubiquitinating enzyme."
      Janssen J.W.G., Schleithhoff L., Bartram C.R., Schulz A.S.
      Oncogene 16:1767-1772(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: NIH Swiss.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Mammary gland.
    4. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    6. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
      Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
      J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Mast cell.
    7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    8. "NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons."
      Yokoyama K., Tezuka T., Kotani M., Nakazawa T., Hoshina N., Shimoda Y., Kakuta S., Sudo K., Watanabe K., Iwakura Y., Yamamoto T.
      EMBO J. 30:4739-4754(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NYAP1; NYAP2 AND MYO16.

    Entry informationi

    Entry nameiP85B_MOUSE
    AccessioniPrimary (citable) accession number: O08908
    Secondary accession number(s): Q5U3K7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3