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O08900 (IKZF3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein Aiolos
Alternative name(s):
Ikaros family zinc finger protein 3
Gene names
Name:Ikzf3
Synonyms:Zfpn1a3, Znfn1a3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length507 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Binds to GGGAA. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner. Ref.1 Ref.4

Subunit structure

Homodimer, and heterodimer with other IKAROS family members. Interacts with IKZF4 AND IKZF5. Interacts with HRAS. Interacts with FOXP3; this interaction may be required for silencing target genes and regulating the suppressive activity of FOXP3-positive regulatory T-cells (Treg). Interacts with BCL21L isoform Bcl-X(L);this interaction blocks the anti-apoptotic role of BCL21L. Associates with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A By similarity. Interacts with IKZF1. Ref.1

Subcellular location

Nucleus. Cytoplasm Ref.1.

Tissue specificity

Expression is restricted to lymphoid tissues. Expressed at highest levels in spleen and at lower levels in the thymus and bone marrow. First detected in more committed lymphoid progenitors and strongly up-regulated as these differentiate into pre-T and pre-B cell precursors. Ref.1

Sequence similarities

Belongs to the Ikaros C2H2-type zinc-finger protein family.

Contains 6 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processB-cell activation
Transcription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processB cell activation

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of B cell differentiation

Inferred from mutant phenotype Ref.4. Source: UniProtKB

regulation of B cell proliferation

Inferred from mutant phenotype Ref.4. Source: UniProtKB

regulation of apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of lymphocyte differentiation

Inferred from mutant phenotype Ref.1. Source: UniProtKB

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from direct assay Ref.1. Source: UniProtKB

nucleus

Inferred from direct assay Ref.1. Source: UniProtKB

plasma membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction Ref.1. Source: UniProtKB

protein heterodimerization activity

Inferred from physical interaction PubMed 9560339. Source: MGI

protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 507507Zinc finger protein Aiolos
PRO_0000047091

Regions

Zinc finger117 – 13923C2H2-type 1
Zinc finger145 – 16723C2H2-type 2
Zinc finger173 – 19523C2H2-type 3
Zinc finger201 – 22323C2H2-type 4; atypical
Zinc finger450 – 47223C2H2-type 5
Zinc finger478 – 50225C2H2-type 6; atypical

Experimental info

Sequence conflict1141G → S in AAB58795. Ref.1
Sequence conflict3191L → F in AAB58795. Ref.1
Sequence conflict3521N → M in AAB58795. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O08900 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 103D46D0C6E236F7

FASTA50757,967
        10         20         30         40         50         60 
MEDIQPTVEL KSTEEQPLPT ESPDALNDYS LPKPHEIENV DSREAPANED EDAGEDSMKV 

        70         80         90        100        110        120 
KDEYSDRDEN IMKPEPMGDA EESEMPYSYA REYSDYESIK LERHVPYDNS RPTGGKMNCD 

       130        140        150        160        170        180 
VCGLSCISFN VLMVHKRSHT GERPFQCNQC GASFTQKGNL LRHIKLHTGE KPFKCHLCNY 

       190        200        210        220        230        240 
ACQRRDALTG HLRTHSVEKP YKCEFCGRSY KQRSSLEEHK ERCRAFLQNP DLGDAASVEA 

       250        260        270        280        290        300 
RHIKAEMGSE RALVLDRLAS NVAKRKSSMP QKFIGEKRHC FDANYNPGYM YEKENEMMQT 

       310        320        330        340        350        360 
RMMDQAINNA ISYLGAEALR PLVQTPPAPT SEMVPVISSV YPIALTRADM PNGAPQEMEK 

       370        380        390        400        410        420 
KRILLPEKIL PSERGLSPNN SAQDSTDTDS NHEDRQHLYQ QSHVVLPQAR NGMPLLKEVP 

       430        440        450        460        470        480 
RSFELLKPPP ICLRDSIKVI NKEGEVMDVF RCDHCHVLFL DYVMFTIHMG CHGFRDPFEC 

       490        500 
NMCGYRSHDR YEFSSHIARG EHRAMLK 

« Hide

References

« Hide 'large scale' references
[1]"Aiolos, a lymphoid restricted transcription factor that interacts with Ikaros to regulate lymphocyte differentiation."
Morgan B., Sun L., Avitahl N., Andrikopoulos K., Ikeda T., Gonzales E., Wu P., Neben S., Georgopoulos K.
EMBO J. 16:2004-2013(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH IKZF1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Aiolos regulates B cell activation and maturation to effector state."
Wang J.H., Avitahl N., Cariappa A., Friedrich C., Ikeda T., Renold A., Andrikopoulos K., Liang L., Pillai S., Morgan B.A., Georgopoulos K.
Immunity 9:543-553(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF001293 mRNA. Translation: AAB58795.1.
AL591390, AL591125 Genomic DNA. Translation: CAM22013.1.
AL591125, AL591390 Genomic DNA. Translation: CAM24385.1.
CH466556 Genomic DNA. Translation: EDL16146.1.
CCDSCCDS25352.1.
RefSeqNP_035901.1. NM_011771.1.
UniGeneMm.133367.
Mm.459157.

3D structure databases

ProteinModelPortalO08900.
SMRO08900. Positions 85-220, 447-503.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204702. 4 interactions.
IntActO08900. 3 interactions.

PTM databases

PhosphoSiteO08900.

Proteomic databases

PaxDbO08900.
PRIDEO08900.

Protocols and materials databases

DNASU22780.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000103141; ENSMUSP00000099430; ENSMUSG00000018168.
GeneID22780.
KEGGmmu:22780.
UCSCuc007lgl.1. mouse.

Organism-specific databases

CTD22806.
MGIMGI:1342542. Ikzf3.

Phylogenomic databases

eggNOGNOG274203.
GeneTreeENSGT00550000074392.
HOGENOMHOG000049114.
HOVERGENHBG004752.
InParanoidB1AQE6.
KOK09220.
OMAYPIALTR.
OrthoDBEOG79GT61.
TreeFamTF331189.

Gene expression databases

ArrayExpressO08900.
BgeeO08900.
CleanExMM_IKZF3.
GenevestigatorO08900.

Family and domain databases

Gene3D3.30.160.60. 4 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIKZF3. mouse.
NextBio303343.
PROO08900.
SOURCESearch...

Entry information

Entry nameIKZF3_MOUSE
AccessionPrimary (citable) accession number: O08900
Secondary accession number(s): B1AQE6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot