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Protein

Zinc finger protein Aiolos

Gene

Ikzf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Binds to GGGAA. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri117 – 13923C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri145 – 16723C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri173 – 19523C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri201 – 22323C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri450 – 47223C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri478 – 50225C2H2-type 6; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein heterodimerization activity Source: MGI
  3. protein homodimerization activity Source: UniProtKB
  4. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  5. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
  6. sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  1. B cell activation Source: UniProtKB-KW
  2. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  3. regulation of apoptotic process Source: UniProtKB
  4. regulation of B cell differentiation Source: UniProtKB
  5. regulation of B cell proliferation Source: UniProtKB
  6. regulation of lymphocyte differentiation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

B-cell activation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein Aiolos
Alternative name(s):
Ikaros family zinc finger protein 3
Gene namesi
Name:Ikzf3
Synonyms:Zfpn1a3, Znfn1a3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1342542. Ikzf3.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
  3. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 507507Zinc finger protein AiolosPRO_0000047091Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei325 – 3251PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO08900.
PRIDEiO08900.

PTM databases

PhosphoSiteiO08900.

Expressioni

Tissue specificityi

Expression is restricted to lymphoid tissues. Expressed at highest levels in spleen and at lower levels in the thymus and bone marrow. First detected in more committed lymphoid progenitors and strongly up-regulated as these differentiate into pre-T and pre-B cell precursors.1 Publication

Gene expression databases

BgeeiO08900.
CleanExiMM_IKZF3.
ExpressionAtlasiO08900. baseline and differential.
GenevestigatoriO08900.

Interactioni

Subunit structurei

Homodimer, and heterodimer with other IKAROS family members. Interacts with IKZF4 AND IKZF5. Interacts with HRAS. Interacts with FOXP3; this interaction may be required for silencing target genes and regulating the suppressive activity of FOXP3-positive regulatory T-cells (Treg). Interacts with BCL21L isoform Bcl-X(L); this interaction blocks the anti-apoptotic role of BCL21L. Associates with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A (By similarity). Interacts with IKZF1.By similarity1 Publication

Protein-protein interaction databases

BioGridi204702. 4 interactions.
IntActiO08900. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliO08900.
SMRiO08900. Positions 115-220, 441-503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 6 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri117 – 13923C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri145 – 16723C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri173 – 19523C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri201 – 22323C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri450 – 47223C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri478 – 50225C2H2-type 6; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG274203.
GeneTreeiENSGT00550000074392.
HOGENOMiHOG000049114.
HOVERGENiHBG004752.
InParanoidiO08900.
KOiK09220.
OMAiYPIALTR.
OrthoDBiEOG79GT61.
TreeFamiTF331189.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08900-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDIQPTVEL KSTEEQPLPT ESPDALNDYS LPKPHEIENV DSREAPANED
60 70 80 90 100
EDAGEDSMKV KDEYSDRDEN IMKPEPMGDA EESEMPYSYA REYSDYESIK
110 120 130 140 150
LERHVPYDNS RPTGGKMNCD VCGLSCISFN VLMVHKRSHT GERPFQCNQC
160 170 180 190 200
GASFTQKGNL LRHIKLHTGE KPFKCHLCNY ACQRRDALTG HLRTHSVEKP
210 220 230 240 250
YKCEFCGRSY KQRSSLEEHK ERCRAFLQNP DLGDAASVEA RHIKAEMGSE
260 270 280 290 300
RALVLDRLAS NVAKRKSSMP QKFIGEKRHC FDANYNPGYM YEKENEMMQT
310 320 330 340 350
RMMDQAINNA ISYLGAEALR PLVQTPPAPT SEMVPVISSV YPIALTRADM
360 370 380 390 400
PNGAPQEMEK KRILLPEKIL PSERGLSPNN SAQDSTDTDS NHEDRQHLYQ
410 420 430 440 450
QSHVVLPQAR NGMPLLKEVP RSFELLKPPP ICLRDSIKVI NKEGEVMDVF
460 470 480 490 500
RCDHCHVLFL DYVMFTIHMG CHGFRDPFEC NMCGYRSHDR YEFSSHIARG

EHRAMLK
Length:507
Mass (Da):57,967
Last modified:July 27, 2011 - v2
Checksum:i103D46D0C6E236F7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141G → S in AAB58795 (PubMed:9155026).Curated
Sequence conflicti319 – 3191L → F in AAB58795 (PubMed:9155026).Curated
Sequence conflicti352 – 3521N → M in AAB58795 (PubMed:9155026).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001293 mRNA. Translation: AAB58795.1.
AL591390, AL591125 Genomic DNA. Translation: CAM22013.1.
AL591125, AL591390 Genomic DNA. Translation: CAM24385.1.
CH466556 Genomic DNA. Translation: EDL16146.1.
CCDSiCCDS25352.1.
RefSeqiNP_035901.1. NM_011771.1.
UniGeneiMm.133367.
Mm.459157.

Genome annotation databases

EnsembliENSMUST00000103141; ENSMUSP00000099430; ENSMUSG00000018168.
GeneIDi22780.
KEGGimmu:22780.
UCSCiuc007lgl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001293 mRNA. Translation: AAB58795.1.
AL591390, AL591125 Genomic DNA. Translation: CAM22013.1.
AL591125, AL591390 Genomic DNA. Translation: CAM24385.1.
CH466556 Genomic DNA. Translation: EDL16146.1.
CCDSiCCDS25352.1.
RefSeqiNP_035901.1. NM_011771.1.
UniGeneiMm.133367.
Mm.459157.

3D structure databases

ProteinModelPortaliO08900.
SMRiO08900. Positions 115-220, 441-503.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204702. 4 interactions.
IntActiO08900. 3 interactions.

PTM databases

PhosphoSiteiO08900.

Proteomic databases

PaxDbiO08900.
PRIDEiO08900.

Protocols and materials databases

DNASUi22780.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103141; ENSMUSP00000099430; ENSMUSG00000018168.
GeneIDi22780.
KEGGimmu:22780.
UCSCiuc007lgl.1. mouse.

Organism-specific databases

CTDi22806.
MGIiMGI:1342542. Ikzf3.

Phylogenomic databases

eggNOGiNOG274203.
GeneTreeiENSGT00550000074392.
HOGENOMiHOG000049114.
HOVERGENiHBG004752.
InParanoidiO08900.
KOiK09220.
OMAiYPIALTR.
OrthoDBiEOG79GT61.
TreeFamiTF331189.

Miscellaneous databases

ChiTaRSiIkzf3. mouse.
NextBioi303343.
PROiO08900.
SOURCEiSearch...

Gene expression databases

BgeeiO08900.
CleanExiMM_IKZF3.
ExpressionAtlasiO08900. baseline and differential.
GenevestigatoriO08900.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Aiolos, a lymphoid restricted transcription factor that interacts with Ikaros to regulate lymphocyte differentiation."
    Morgan B., Sun L., Avitahl N., Andrikopoulos K., Ikeda T., Gonzales E., Wu P., Neben S., Georgopoulos K.
    EMBO J. 16:2004-2013(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH IKZF1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: FUNCTION.

Entry informationi

Entry nameiIKZF3_MOUSE
AccessioniPrimary (citable) accession number: O08900
Secondary accession number(s): B1AQE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: July 27, 2011
Last modified: March 4, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.