O08899 (RGS4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulator of G-protein signaling 4
Short name=RGS4

Gene names

Name:

Rgs4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein By similarity.
Tissue specificity	Expressed at high levels in brain, moderately low levels in heart, and very low levels in lung, liver, and skeletal muscle.
Post-translational modification	Either Cys-2 or Cys-12 or both are palmitoylated By similarity. Phosphorylated by cyclic GMP-dependent protein kinase By similarity.
Sequence similarities	Contains 1 RGS domain.

Ontologies

Keywords
Molecular function	Signal transduction inhibitor
PTM	Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Traceable author statement PubMed 11342430. Source: MGI termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	GTPase activator activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	Regulator of G-protein signaling 4		PRO_0000204186
Regions
	Domain	62 – 178	117	RGS
Amino acid modifications
	Lipidation	2	1	S-palmitoyl cysteine By similarity
	Lipidation	12	1	S-palmitoyl cysteine By similarity
	Lipidation	95	1	S-palmitoyl cysteine By similarity
Experimental info
	Sequence conflict	162	1	R → K in AAH03882. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O08899 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 5D79581711A1F67C	FASTA	205	23,289
	10 20 30 40 50 60 MCKGLAGLPA SCLRSAKDMK HRLGFLLQKS DSCEHSSSHS KKDKVVTCQR VSQEEVKKWA 70 80 90 100 110 120 ESLENLIHHE CGLAAFKAFL KSEYSEENID FWISCEEYKK IKSPSKLSPK AKKIYNEFIS 130 140 150 160 170 180 VQATKEVNLD SCTREETSRN MLQPTITCFD EAQKKIFNLM ERDSYRRFLK SRFYLDLTNP 190 200 SSCGAEKQKG AKSSADCTSL VSQCA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Distribution of RGS4 mRNA in mouse brain shown by in situ hybridization." Nomoto S., Adachi K., Yang L.X., Hirata Y., Muraguchi S., Kiuchi K. Biochem. Biophys. Res. Commun. 241:281-287(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Substantia nigra.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB004315 mRNA. Translation: BAA20400.1. BC003882 mRNA. Translation: AAH03882.1.
IPI	IPI00116495.
RefSeq	NP_033088.2. NM_009062.3.
UniGene	Mm.41642.
3D structure databases
ProteinModelPortal	O08899.
SMR	O08899. Positions 51-178.
ModBase	Search...
PTM databases
PhosphoSite	O08899.
Proteomic databases
PaxDb	O08899.
PRIDE	O08899.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	19736.
KEGG	mmu:19736.
Organism-specific databases
CTD	5999.
MGI	MGI:108409. Rgs4.
Phylogenomic databases
eggNOG	NOG318241.
HOGENOM	HOG000233512.
HOVERGEN	HBG013233.
InParanoid	O08899.
KO	K16449.
OrthoDB	EOG4PK297.
Gene expression databases
CleanEx	MM_RGS4.
Genevestigator	O08899.
GermOnline	ENSMUSG00000038530. Mus musculus.
Family and domain databases
Gene3D	1.10.196.10. 2 hits.
InterPro	IPR000342. Regulat_G_prot_signal. IPR024066. Regulat_G_prot_signal_dom1. IPR016137. Regulat_G_prot_signal_superfam. [Graphical view]
Pfam	PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR01301. RGSPROTEIN.
SMART	SM00315. RGS. 1 hit. [Graphical view]
SUPFAM	SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RGS4. mouse.
NextBio	297176.
SOURCE	Search...

Entry information

Entry name

RGS4_MOUSE

Accession

Primary (citable) accession number: O08899
Secondary accession number(s): Q99L30

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program