O08849 (RGS2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulator of G-protein signaling 2
Short name=RGS2

Gene names

Name:

Rgs2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. May play a role in leukemogenesis. Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein.
Subunit structure	Interacts with EIF2B5 By similarity. Interacts with PRKG1 (isoform alpha) By similarity.
Subcellular location	Cell membrane By similarity. Cytoplasm By similarity. Nucleus › nucleolus By similarity.
Tissue specificity	Expressed in a wide variety of tissues.
Post-translational modification	Phosphorylated by protein kinase C By similarity. Phosphorylation by PRKG1 leads to activation of RGS2 activity By similarity.
Sequence similarities	Contains 1 RGS domain.

Ontologies

Keywords
Biological process	Cell cycle Translation regulation
Cellular component	Cell membrane Cytoplasm Membrane Nucleus
Molecular function	Signal transduction inhibitor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Traceable author statement PubMed 11342430. Source: MGI brown fat cell differentiation Inferred from direct assay PubMed 18492766. Source: MGI cell cycle Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of G-protein coupled receptor protein signaling pathway Inferred from mutant phenotype PubMed 19127022. Source: BHF-UCL negative regulation of MAP kinase activity Inferred from mutant phenotype PubMed 19127022. Source: BHF-UCL negative regulation of cardiac muscle hypertrophy Inferred from mutant phenotype PubMed 19127022. Source: BHF-UCL negative regulation of phospholipase activity Inferred from mutant phenotype PubMed 19127022. Source: BHF-UCL positive regulation of cardiac muscle contraction Inferred from mutant phenotype PubMed 19127022PubMed 21291891. Source: BHF-UCL regulation of adrenergic receptor signaling pathway Inferred from mutant phenotype PubMed 21291891. Source: BHF-UCL regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW relaxation of cardiac muscle Inferred from mutant phenotype PubMed 19127022. Source: BHF-UCL relaxation of vascular smooth muscle Inferred from mutant phenotype PubMed 14608379. Source: UniProtKB spermatogenesis Inferred from expression pattern PubMed 14581517. Source: BHF-UCL termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from mutant phenotype PubMed 19127022. Source: BHF-UCL internal side of plasma membrane Inferred from direct assay PubMed 21291891. Source: BHF-UCL nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Traceable author statement PubMed 11342430. Source: MGI
Molecular_function	GTPase activator activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	Regulator of G-protein signaling 2		PRO_0000204179
Regions
	Domain	83 – 199	117	RGS
	Region	32 – 66	35	Necessary for membrane association By similarity
	Region	79 – 116	38	Necessary to inhibit protein synthesis By similarity
Experimental info
	Sequence conflict	39 – 40	2	KD → NH in AAB50617. Ref.1
	Sequence conflict	78 – 79	2	QL → DV in AAL28114. Ref.3
	Sequence conflict	78 – 79	2	QL → HV in AAB50617. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O08849 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: 5D6E255C2BC7E7FA	FASTA	211	24,294
	10 20 30 40 50 60 MQSAMFLAVQ HDCVPMDKSA GNGPKVEEKR EKMKRTLLKD WKTRLSYFLQ NSSAPGKPKT 70 80 90 100 110 120 GKKSKQQTFI KPSPEEAQLW AEAFDELLAS KYGLAAFRAF LKSEFCEENI EFWLACEDFK 130 140 150 160 170 180 KTKSPQKLSS KARKIYTDFI EKEAPKEINI DFQTKSLIAQ NIQEATSGCF TTAQKRVYSL 190 200 210 MENNSYPRFL ESEFYQDLCK KPQITTEPHA T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a novel mammalian RGS protein that binds to Galpha proteins and inhibits pheromone signaling in yeast." Chen C., Zheng B., Han J., Lin S.-C. J. Biol. Chem. 272:8679-8685(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"RGS molecule expression in murine B lymphocytes and ability to down-regulate chemotaxis to lymphoid chemokines." Reif K., Cyster J.G. J. Immunol. 164:4720-4729(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[3]	"Identification of novel endometrial and embryonic factors involved in mouse embryo implantation." Shen Q.-X., Wang J., Huang Z.-P. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Testis.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U67187 mRNA. Translation: AAB50617.1. AF215668 mRNA. Translation: AAF34625.1. AF432916 mRNA. Translation: AAL28114.1. AK031603 mRNA. Translation: BAC27471.1. AK077922 mRNA. Translation: BAC37065.1. AK162276 mRNA. Translation: BAE36830.1. BC023001 mRNA. Translation: AAH23001.1.
IPI	IPI00309304.
RefSeq	NP_033087.2. NM_009061.4.
UniGene	Mm.28262.
3D structure databases
ProteinModelPortal	O08849.
SMR	O08849. Positions 70-203.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4953705.
STRING	10090.ENSMUSP00000115558.
PTM databases
PhosphoSite	O08849.
Proteomic databases
PRIDE	O08849.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000127206; ENSMUSP00000115558; ENSMUSG00000026360.
GeneID	19735.
KEGG	mmu:19735.
Organism-specific databases
CTD	5997.
MGI	MGI:1098271. Rgs2.
Phylogenomic databases
eggNOG	NOG252352.
GeneTree	ENSGT00700000104212.
HOGENOM	HOG000233512.
HOVERGEN	HBG013233.
InParanoid	O08849.
KO	K16449.
OMA	KSKQQAF.
OrthoDB	EOG4ZGPD8.
Gene expression databases
ArrayExpress	O08849.
Bgee	O08849.
CleanEx	MM_RGS2.
Genevestigator	O08849.
GermOnline	ENSMUSG00000026360. Mus musculus.
Family and domain databases
Gene3D	1.10.196.10. 2 hits.
InterPro	IPR000342. Regulat_G_prot_signal. IPR024066. Regulat_G_prot_signal_dom1. IPR016137. Regulat_G_prot_signal_superfam. [Graphical view]
Pfam	PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR01301. RGSPROTEIN.
SMART	SM00315. RGS. 1 hit. [Graphical view]
SUPFAM	SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	297172.
SOURCE	Search...

Entry information

Entry name

RGS2_MOUSE

Accession

Primary (citable) accession number: O08849
Secondary accession number(s): Q544S7, Q91WX1, Q9JL24

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 14, 2001
Last modified:	May 1, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program