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Protein

Regulator of G-protein signaling 2

Gene

Rgs2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. May play a role in leukemogenesis. Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein.

GO - Molecular functioni

  1. GTPase activator activity Source: GO_Central

GO - Biological processi

  1. brown fat cell differentiation Source: MGI
  2. cell cycle Source: UniProtKB-KW
  3. G-protein coupled receptor signaling pathway Source: MGI
  4. negative regulation of cAMP-mediated signaling Source: MGI
  5. negative regulation of cardiac muscle hypertrophy Source: BHF-UCL
  6. negative regulation of G-protein coupled receptor protein signaling pathway Source: BHF-UCL
  7. negative regulation of MAP kinase activity Source: BHF-UCL
  8. negative regulation of phospholipase activity Source: BHF-UCL
  9. positive regulation of cardiac muscle contraction Source: BHF-UCL
  10. positive regulation of microtubule polymerization Source: Ensembl
  11. regulation of adrenergic receptor signaling pathway Source: BHF-UCL
  12. regulation of translation Source: UniProtKB-KW
  13. relaxation of cardiac muscle Source: BHF-UCL
  14. relaxation of vascular smooth muscle Source: UniProtKB
  15. spermatogenesis Source: BHF-UCL
  16. termination of G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Signal transduction inhibitor

Keywords - Biological processi

Cell cycle, Translation regulation

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 2
Short name:
RGS2
Gene namesi
Name:Rgs2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1098271. Rgs2.

Subcellular locationi

Cell membrane By similarity. Cytoplasm By similarity. Nucleusnucleolus By similarity

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. cytoplasmic side of plasma membrane Source: BHF-UCL
  3. cytosol Source: BHF-UCL
  4. neuron projection Source: Ensembl
  5. nucleolus Source: UniProtKB-SubCell
  6. nucleus Source: MGI
  7. plasma membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Regulator of G-protein signaling 2PRO_0000204179Add
BLAST

Post-translational modificationi

Phosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO08849.

PTM databases

PhosphoSiteiO08849.

Expressioni

Tissue specificityi

Expressed in a wide variety of tissues.

Gene expression databases

BgeeiO08849.
CleanExiMM_RGS2.
GenevestigatoriO08849.

Interactioni

Subunit structurei

Interacts with EIF2B5. Interacts with PRKG1 (isoform alpha).By similarity

Protein-protein interaction databases

IntActiO08849. 2 interactions.
MINTiMINT-4953705.
STRINGi10090.ENSMUSP00000115558.

Structurei

3D structure databases

ProteinModelPortaliO08849.
SMRiO08849. Positions 73-200.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini83 – 199117RGSPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni32 – 6635Necessary for membrane associationBy similarityAdd
BLAST
Regioni79 – 11638Necessary to inhibit protein synthesisBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG252352.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiO08849.
KOiK18154.
OMAiQQAFIKP.
OrthoDBiEOG7VHSZ5.
PhylomeDBiO08849.
TreeFamiTF315837.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08849-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSAMFLAVQ HDCVPMDKSA GNGPKVEEKR EKMKRTLLKD WKTRLSYFLQ
60 70 80 90 100
NSSAPGKPKT GKKSKQQTFI KPSPEEAQLW AEAFDELLAS KYGLAAFRAF
110 120 130 140 150
LKSEFCEENI EFWLACEDFK KTKSPQKLSS KARKIYTDFI EKEAPKEINI
160 170 180 190 200
DFQTKSLIAQ NIQEATSGCF TTAQKRVYSL MENNSYPRFL ESEFYQDLCK
210
KPQITTEPHA T
Length:211
Mass (Da):24,294
Last modified:August 14, 2001 - v2
Checksum:i5D6E255C2BC7E7FA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 402KD → NH in AAB50617 (PubMed:9079700).Curated
Sequence conflicti78 – 792QL → DV in AAL28114 (Ref. 3) Curated
Sequence conflicti78 – 792QL → HV in AAB50617 (PubMed:9079700).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67187 mRNA. Translation: AAB50617.1.
AF215668 mRNA. Translation: AAF34625.1.
AF432916 mRNA. Translation: AAL28114.1.
AK031603 mRNA. Translation: BAC27471.1.
AK077922 mRNA. Translation: BAC37065.1.
AK162276 mRNA. Translation: BAE36830.1.
BC023001 mRNA. Translation: AAH23001.1.
CCDSiCCDS15347.1.
RefSeqiNP_033087.2. NM_009061.4.
UniGeneiMm.28262.

Genome annotation databases

EnsembliENSMUST00000127206; ENSMUSP00000115558; ENSMUSG00000026360.
GeneIDi19735.
KEGGimmu:19735.
UCSCiuc007cxg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67187 mRNA. Translation: AAB50617.1.
AF215668 mRNA. Translation: AAF34625.1.
AF432916 mRNA. Translation: AAL28114.1.
AK031603 mRNA. Translation: BAC27471.1.
AK077922 mRNA. Translation: BAC37065.1.
AK162276 mRNA. Translation: BAE36830.1.
BC023001 mRNA. Translation: AAH23001.1.
CCDSiCCDS15347.1.
RefSeqiNP_033087.2. NM_009061.4.
UniGeneiMm.28262.

3D structure databases

ProteinModelPortaliO08849.
SMRiO08849. Positions 73-200.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO08849. 2 interactions.
MINTiMINT-4953705.
STRINGi10090.ENSMUSP00000115558.

PTM databases

PhosphoSiteiO08849.

Proteomic databases

PRIDEiO08849.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000127206; ENSMUSP00000115558; ENSMUSG00000026360.
GeneIDi19735.
KEGGimmu:19735.
UCSCiuc007cxg.2. mouse.

Organism-specific databases

CTDi5997.
MGIiMGI:1098271. Rgs2.

Phylogenomic databases

eggNOGiNOG252352.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiO08849.
KOiK18154.
OMAiQQAFIKP.
OrthoDBiEOG7VHSZ5.
PhylomeDBiO08849.
TreeFamiTF315837.

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.

Miscellaneous databases

NextBioi297172.
PROiO08849.
SOURCEiSearch...

Gene expression databases

BgeeiO08849.
CleanExiMM_RGS2.
GenevestigatoriO08849.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a novel mammalian RGS protein that binds to Galpha proteins and inhibits pheromone signaling in yeast."
    Chen C., Zheng B., Han J., Lin S.-C.
    J. Biol. Chem. 272:8679-8685(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "RGS molecule expression in murine B lymphocytes and ability to down-regulate chemotaxis to lymphoid chemokines."
    Reif K., Cyster J.G.
    J. Immunol. 164:4720-4729(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. "Identification of novel endometrial and embryonic factors involved in mouse embryo implantation."
    Shen Q.-X., Wang J., Huang Z.-P.
    Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Retina.

Entry informationi

Entry nameiRGS2_MOUSE
AccessioniPrimary (citable) accession number: O08849
Secondary accession number(s): Q544S7, Q91WX1, Q9JL24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 14, 2001
Last modified: February 4, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.