Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myc box-dependent-interacting protein 1

Gene

Bin1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.1 Publication

GO - Molecular functioni

  • GTPase binding Source: RGD
  • protein complex binding Source: RGD
  • protein heterodimerization activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Myc box-dependent-interacting protein 1
Alternative name(s):
Amphiphysin II
Amphiphysin-like protein
Bridging integrator 1
Gene namesi
Name:Bin1
Synonyms:Amph2, Amphl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621786. Bin1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: Alzheimers_University_of_Toronto
  • axon initial segment Source: Alzheimers_University_of_Toronto
  • axon terminus Source: RGD
  • cerebellar mossy fiber Source: RGD
  • I band Source: Alzheimers_University_of_Toronto
  • lipid tube Source: Alzheimers_University_of_Toronto
  • microtubule Source: Alzheimers_University_of_Toronto
  • node of Ranvier Source: Alzheimers_University_of_Toronto
  • nucleus Source: UniProtKB-SubCell
  • synaptic vesicle Source: RGD
  • T-tubule Source: Alzheimers_University_of_Toronto
  • varicosity Source: RGD
  • Z disc Source: Alzheimers_University_of_Toronto
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001929532 – 588Myc box-dependent-interacting protein 1Add BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei296PhosphoserineBy similarity1
Modified residuei298PhosphoserineCombined sources1
Modified residuei304PhosphoserineCombined sources1
Modified residuei308PhosphothreonineCombined sources1
Modified residuei324PhosphoserineCombined sources1
Modified residuei332PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by protein kinase C.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO08839.
PRIDEiO08839.

PTM databases

iPTMnetiO08839.
PhosphoSitePlusiO08839.

Expressioni

Tissue specificityi

Isoform AMPH2-1 is expressed in brain, concentrated at nerve terminals. Isoform AMPH2-2 is widely expressed.

Gene expression databases

BgeeiENSRNOG00000012852.

Interactioni

Subunit structurei

Heterodimer with AMPH. Binds SH3GLB1 (By similarity). Interacts (via SH3 domain) with SYNJ1. Interacts (via SH3 domain) with DNM1. Interacts with CLTC. Interacts with AP2A2. Interacts with AP2B1. Interacts with MYC (via N-terminal transactivation domain); the interaction requires the integrity of the conserved MYC box regions 1 and 2. Interacts with BIN2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
AmphO088382EBI-80095,EBI-80080
Dnm1P215752EBI-80095,EBI-80070
Necap1Q9CR958EBI-80095,EBI-7592476From a different organism.

GO - Molecular functioni

  • GTPase binding Source: RGD
  • protein complex binding Source: RGD
  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

BioGridi250629. 13 interactors.
DIPiDIP-30979N.
IntActiO08839. 8 interactors.
MINTiMINT-101225.
STRINGi10116.ENSRNOP00000017573.

Structurei

Secondary structure

1588
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi518 – 524Combined sources7
Beta strandi541 – 545Combined sources5
Helixi550 – 552Combined sources3
Beta strandi557 – 562Combined sources6
Helixi563 – 567Combined sources5
Helixi572 – 575Combined sources4
Beta strandi577 – 580Combined sources4
Helixi581 – 583Combined sources3
Beta strandi584 – 587Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BB9X-ray2.20A495-588[»]
ProteinModelPortaliO08839.
SMRiO08839.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08839.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 276BARPROSITE-ProRule annotationAdd BLAST248
Domaini515 – 588SH3PROSITE-ProRule annotationAdd BLAST74

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 122Interaction with BIN2By similarityAdd BLAST121
Regioni379 – 422Clathrin-bindingBy similarityAdd BLAST44

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili15 – 42Sequence analysisAdd BLAST28
Coiled coili193 – 274Sequence analysisAdd BLAST82

Sequence similaritiesi

Contains 1 BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiENOG410ITR4. Eukaryota.
ENOG410XQXT. LUCA.
HOGENOMiHOG000252987.
HOVERGENiHBG004224.
InParanoidiO08839.
KOiK12562.
PhylomeDBiO08839.

Family and domain databases

Gene3Di1.20.1270.60. 2 hits.
InterProiIPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR003023. Amphiphysin_2.
IPR004148. BAR_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF03114. BAR. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR01251. AMPHIPHYSIN.
PR01253. AMPHIPHYSIN2.
PR00452. SH3DOMAIN.
SMARTiSM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform AMPH2-1 (identifier: O08839-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEMGSKGVT AGKIASNVQK KLTRAQEKVL QKLGKADETK DEQFEQCVQN
60 70 80 90 100
FNKQLTEGTR LQKDLRTYLA SVKAMHEASK KLSECLQEVY EPEWPGRDEA
110 120 130 140 150
NKIAENNDLL WMDYHQKLVD QALLTMDTYL GQFPDIKSRI AKRGRKLVDY
160 170 180 190 200
DSARHHYESL QTAKKKDEAK IAKPVSLLEK AAPQWCQGKL QAHLVAQTNL
210 220 230 240 250
LRNQAEEELI KAQKVFEEMN VDLQEELPSL WNSRVGFYVN TFQSIAGLEE
260 270 280 290 300
NFHKEMSKLN QNLNDVLVSL EKQHGSNTFT VKAQPSDSAP EKGNKSPSPP
310 320 330 340 350
PDGSPAATPE IRVNHEPEPA SGASPGATIP KSPSQLRKGP PVPPPPKHTP
360 370 380 390 400
SKEMKQEQIL SLFDDAFVPE ISVTTPSQFE APGPFSEQAS LLDLDFEPLP
410 420 430 440 450
PVASPVKAPT PSGQSIPWDL WEPTESQAGV LPSGEPSSAE GSFAVAWPSQ
460 470 480 490 500
TAEPGPAQPA EASEVVGGTQ EPGETAASEA TSSSLPAVVV ETFSATVNGA
510 520 530 540 550
VEGSTTTGRL DLPPGFMFKV QAQHDYTATD TDELQLKAGD VVLVIPFQNP
560 570 580
EEQDEGWLMG VKESDWNQHK ELEKCRGVFP ENFTERVQ
Length:588
Mass (Da):64,533
Last modified:July 1, 1997 - v1
Checksum:i164AC90E09547F1A
GO
Isoform AMPH2-2 (identifier: O08839-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     423-460: Missing.

Show »
Length:550
Mass (Da):60,857
Checksum:iEABCC9BFA01853A3
GO
Isoform AMPH2-3 (identifier: O08839-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     335-588: Missing.

Show »
Length:334
Mass (Da):37,377
Checksum:i7DFCA41A3235D22E
GO
Isoform AMPH2-4 (identifier: O08839-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     173-205: Missing.
     253-588: Missing.

Show »
Length:219
Mass (Da):25,238
Checksum:iA780685B678CD85B
GO
Isoform AMPH2-5 (identifier: O08839-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     335-482: Missing.

Show »
Length:440
Mass (Da):49,143
Checksum:i63E65E87050D4DF4
GO
Isoform AMPH2-6 (identifier: O08839-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     173-205: Missing.
     335-482: Missing.

Show »
Length:407
Mass (Da):45,506
Checksum:i046474D7A2E23EDB
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000256173 – 205Missing in isoform AMPH2-4 and isoform AMPH2-6. CuratedAdd BLAST33
Alternative sequenceiVSP_000257253 – 588Missing in isoform AMPH2-4. CuratedAdd BLAST336
Alternative sequenceiVSP_000258335 – 588Missing in isoform AMPH2-3. CuratedAdd BLAST254
Alternative sequenceiVSP_000259335 – 482Missing in isoform AMPH2-5 and isoform AMPH2-6. CuratedAdd BLAST148
Alternative sequenceiVSP_000260423 – 460Missing in isoform AMPH2-2. CuratedAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13380 mRNA. Translation: CAA73807.1.
RefSeqiNP_446411.1. NM_053959.1. [O08839-1]
UniGeneiRn.17098.

Genome annotation databases

GeneIDi117028.
KEGGirno:117028.
UCSCiRGD:621786. rat. [O08839-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13380 mRNA. Translation: CAA73807.1.
RefSeqiNP_446411.1. NM_053959.1. [O08839-1]
UniGeneiRn.17098.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BB9X-ray2.20A495-588[»]
ProteinModelPortaliO08839.
SMRiO08839.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250629. 13 interactors.
DIPiDIP-30979N.
IntActiO08839. 8 interactors.
MINTiMINT-101225.
STRINGi10116.ENSRNOP00000017573.

PTM databases

iPTMnetiO08839.
PhosphoSitePlusiO08839.

Proteomic databases

PaxDbiO08839.
PRIDEiO08839.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi117028.
KEGGirno:117028.
UCSCiRGD:621786. rat. [O08839-1]

Organism-specific databases

CTDi274.
RGDi621786. Bin1.

Phylogenomic databases

eggNOGiENOG410ITR4. Eukaryota.
ENOG410XQXT. LUCA.
HOGENOMiHOG000252987.
HOVERGENiHBG004224.
InParanoidiO08839.
KOiK12562.
PhylomeDBiO08839.

Miscellaneous databases

EvolutionaryTraceiO08839.
PROiO08839.

Gene expression databases

BgeeiENSRNOG00000012852.

Family and domain databases

Gene3Di1.20.1270.60. 2 hits.
InterProiIPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR003023. Amphiphysin_2.
IPR004148. BAR_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF03114. BAR. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR01251. AMPHIPHYSIN.
PR01253. AMPHIPHYSIN2.
PR00452. SH3DOMAIN.
SMARTiSM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIN1_RAT
AccessioniPrimary (citable) accession number: O08839
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.