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O08838

- AMPH_RAT

UniProt

O08838 - AMPH_RAT

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Protein

Amphiphysin

Gene

Amph

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton (By similarity).By similarity

GO - Molecular functioni

  1. protein complex binding Source: RGD
  2. protein C-terminus binding Source: RGD
  3. protein heterodimerization activity Source: RGD

GO - Biological processi

  1. positive regulation of endocytosis Source: RGD
  2. positive regulation of GTPase activity Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Amphiphysin
Gene namesi
Name:Amph
Synonyms:Amph1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620274. Amph.

Subcellular locationi

GO - Cellular componenti

  1. axon terminus Source: RGD
  2. cell junction Source: UniProtKB-KW
  3. cytoplasmic vesicle Source: UniProtKB-KW
  4. cytoskeleton Source: UniProtKB-KW
  5. membrane Source: UniProtKB-KW
  6. synapse Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 683683AmphiphysinPRO_0000192949Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei496 – 4961PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO08838.
PRIDEiO08838.

PTM databases

PhosphoSiteiO08838.

Expressioni

Gene expression databases

GenevestigatoriO08838.

Interactioni

Subunit structurei

Heterodimer with BIN1. Binds SH3GLB1 (By similarity). Interacts with REPS1 and SGIP1; may be involved in clathrin-mediated endocytosis (By similarity). Binds AP2A2. Interacts with AP2B1.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Bin1O088392EBI-80080,EBI-80095
Dnm1P215753EBI-80080,EBI-80070
Dnm2P390522EBI-80080,EBI-349613
Necap1Q9CR957EBI-80080,EBI-7592476From a different organism.

Protein-protein interaction databases

BioGridi248889. 18 interactions.
DIPiDIP-30977N.
IntActiO08838. 7 interactions.
MINTiMINT-101171.
STRINGi10116.ENSRNOP00000017102.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VJ0X-ray1.60Q324-330[»]
ProteinModelPortaliO08838.
SMRiO08838. Positions 35-234, 604-683.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08838.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 240217BARPROSITE-ProRule annotationAdd
BLAST
Domaini610 – 68374SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili10 – 8374Sequence AnalysisAdd
BLAST
Coiled coili144 – 19148Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG264615.
HOGENOMiHOG000252987.
HOVERGENiHBG004224.
InParanoidiO08838.
KOiK12562.
PhylomeDBiO08838.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR003017. Amphiphysin_1.
IPR004148. BAR_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF03114. BAR. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR01251. AMPHIPHYSIN.
PR01252. AMPHIPHYSIN1.
PR00452. SH3DOMAIN.
SMARTiSM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08838-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADIKTGIFA KNVQKRLNRA QEKVLQKLGK ADETKDEQFE EYVQNFKRQE
60 70 80 90 100
AEGTRLQREL RGYLAAIKGM QEASMKLTES LHEVYEPDWY GREDVKMVGE
110 120 130 140 150
KCDVLWEDFH QKLVDGSLLT LDTYLGQFPD IKNRIAKRSR KLVDYDSARH
160 170 180 190 200
HLEALQSSKR KDESRISKAE EEFQKAQKVF EEFNVDLQEE LPSLWSRRVG
210 220 230 240 250
FYVNTFKNVS SLEAKFHKEI AVLCHKLYEV MTKLGDQHAD KAFSIQGAPS
260 270 280 290 300
DSGPLRIAKT PSPPEEASPL PSPTASPNHT LAPASPAPVR PRSPSQTRKG
310 320 330 340 350
PPVPPLPKVT PTKELQQENI INFFEDNFVP EINVTTPSQN EVLEVKKEET
360 370 380 390 400
LLDLDFDPFK PDVTPAGSAA ATHSPMSQTL PWDLWTTSTD LVQPASGGSF
410 420 430 440 450
NDFTQPQDTS LFTMQTDQNM AETEQALPTE PQAEEPPTTA AAPTAGLDLG
460 470 480 490 500
LEMEEPKEEA AIPPGTDAGE TVGTEGSTGE EAEAEKAALP AGEGESPEGA
510 520 530 540 550
KIDVESTELA SSESPQAAEL EAGAPQEKVI PSVVIEPASN HEGEEHQETT
560 570 580 590 600
TGTETREATE DVAPQGPAGE KQELATEPTP LDSQAATPAP AGAVDASLSA
610 620 630 640 650
GDAAQELPPG FLYKVETLHD FEAANSDELT LQRGDVVLVV PSDSEADQDA
660 670 680
GWLVGVKESD WLQYRDLATY KGLFPENFTR HLE
Length:683
Mass (Da):74,878
Last modified:July 1, 1997 - v1
Checksum:i7FEA4A9E5A1F6631
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13381 mRNA. Translation: CAA73808.1.
RefSeqiNP_071553.1. NM_022217.1.
UniGeneiRn.44463.

Genome annotation databases

GeneIDi60668.
KEGGirno:60668.
UCSCiRGD:620274. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13381 mRNA. Translation: CAA73808.1 .
RefSeqi NP_071553.1. NM_022217.1.
UniGenei Rn.44463.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2VJ0 X-ray 1.60 Q 324-330 [» ]
ProteinModelPortali O08838.
SMRi O08838. Positions 35-234, 604-683.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248889. 18 interactions.
DIPi DIP-30977N.
IntActi O08838. 7 interactions.
MINTi MINT-101171.
STRINGi 10116.ENSRNOP00000017102.

PTM databases

PhosphoSitei O08838.

Proteomic databases

PaxDbi O08838.
PRIDEi O08838.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 60668.
KEGGi rno:60668.
UCSCi RGD:620274. rat.

Organism-specific databases

CTDi 273.
RGDi 620274. Amph.

Phylogenomic databases

eggNOGi NOG264615.
HOGENOMi HOG000252987.
HOVERGENi HBG004224.
InParanoidi O08838.
KOi K12562.
PhylomeDBi O08838.

Miscellaneous databases

EvolutionaryTracei O08838.
NextBioi 612393.
PROi O08838.

Gene expression databases

Genevestigatori O08838.

Family and domain databases

Gene3Di 1.20.1270.60. 1 hit.
InterProi IPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR003017. Amphiphysin_1.
IPR004148. BAR_dom.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF03114. BAR. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view ]
PRINTSi PR01251. AMPHIPHYSIN.
PR01252. AMPHIPHYSIN1.
PR00452. SH3DOMAIN.
SMARTi SM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Amphiphysin heterodimers: potential role in clathrin-mediated endocytosis."
    Wigge P., Koehler K., Vallis Y., Doyle C., Owen D., Hunt S.P., McMahon H.T.
    Mol. Biol. Cell 8:2003-2015(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain cortex.
  2. Lubec G., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 198-207; 242-256 AND 615-633, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  3. "A structural explanation for the binding of multiple ligands by the alpha-adaptin appendage domain."
    Owen D.J., Vallis Y., Noble M.E.M., Hunter J.B., Dafforn T.R., Evans P.R., McMahon H.T.
    Cell 97:805-815(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AP2A2.
  4. "Crystal structure of the alpha appendage of AP-2 reveals a recruitment platform for clathrin-coat assembly."
    Traub L.M., Downs M.A., Westrich J.L., Fremont D.H.
    Proc. Natl. Acad. Sci. U.S.A. 96:8907-8912(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AP2A2.
  5. "Molecular switches involving the AP-2 beta2 appendage regulate endocytic cargo selection and clathrin coat assembly."
    Edeling M.A., Mishra S.K., Keyel P.A., Steinhauser A.L., Collins B.M., Roth R., Heuser J.E., Owen D.J., Traub L.M.
    Dev. Cell 10:329-342(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AP2B1.
  6. "Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly."
    Schmid E.M., Ford M.G.J., Burtey A., Praefcke G.J.K., Peak-Chew S.-Y., Mills I.G., Benmerah A., McMahon H.T.
    PLoS Biol. 4:E262-E262(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AP2B1.

Entry informationi

Entry nameiAMPH_RAT
AccessioniPrimary (citable) accession number: O08838
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: July 1, 1997
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3