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Protein

Amphiphysin

Gene

Amph

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton (By similarity).By similarity

GO - Molecular functioni

  • ARF guanyl-nucleotide exchange factor activity Source: GO_Central
  • protein complex binding Source: RGD
  • protein C-terminus binding Source: RGD
  • protein heterodimerization activity Source: RGD

GO - Biological processi

  • positive regulation of endocytosis Source: RGD
  • positive regulation of GTPase activity Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Amphiphysin
Gene namesi
Name:Amph
Synonyms:Amph1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620274. Amph.

Subcellular locationi

GO - Cellular componenti

  • axon terminus Source: RGD
  • cell junction Source: UniProtKB-KW
  • cytoskeleton Source: UniProtKB-SubCell
  • synaptic vesicle membrane Source: UniProtKB-SubCell
  • terminal bouton Source: ParkinsonsUK-UCL
  • trans-Golgi network Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001929491 – 683AmphiphysinAdd BLAST683

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei252PhosphoserineCombined sources1
Modified residuei260PhosphothreonineBy similarity1
Modified residuei262PhosphoserineBy similarity1
Modified residuei268PhosphoserineBy similarity1
Modified residuei272PhosphoserineBy similarity1
Modified residuei276PhosphoserineBy similarity1
Modified residuei280PhosphothreonineBy similarity1
Modified residuei496PhosphoserineCombined sources1
Modified residuei511PhosphoserineCombined sources1
Modified residuei512PhosphoserineCombined sources1
Modified residuei514PhosphoserineCombined sources1
Modified residuei626PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO08838.
PRIDEiO08838.

PTM databases

iPTMnetiO08838.
PhosphoSitePlusiO08838.

Interactioni

Subunit structurei

Heterodimer with BIN1. Binds SH3GLB1 (By similarity). Interacts with REPS1 and SGIP1; may be involved in clathrin-mediated endocytosis (By similarity). Binds AP2A2. Interacts with AP2B1.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Bin1O088392EBI-80080,EBI-80095
Dnm1P215753EBI-80080,EBI-80070
Dnm2P390522EBI-80080,EBI-349613
Necap1Q9CR957EBI-80080,EBI-7592476From a different organism.
SRGAP3O432953EBI-80080,EBI-368166From a different organism.

GO - Molecular functioni

  • protein complex binding Source: RGD
  • protein C-terminus binding Source: RGD
  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

BioGridi248889. 19 interactors.
DIPiDIP-30977N.
IntActiO08838. 8 interactors.
MINTiMINT-101171.
STRINGi10116.ENSRNOP00000017102.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VJ0X-ray1.60Q324-330[»]
ProteinModelPortaliO08838.
SMRiO08838.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08838.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 240BARPROSITE-ProRule annotationAdd BLAST217
Domaini610 – 683SH3PROSITE-ProRule annotationAdd BLAST74

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili10 – 83Sequence analysisAdd BLAST74
Coiled coili144 – 191Sequence analysisAdd BLAST48

Sequence similaritiesi

Contains 1 BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG3771. Eukaryota.
ENOG410ZBW1. LUCA.
HOGENOMiHOG000252987.
HOVERGENiHBG004224.
InParanoidiO08838.
KOiK12562.
PhylomeDBiO08838.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR003017. Amphiphysin_1.
IPR004148. BAR_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF03114. BAR. 1 hit.
[Graphical view]
PRINTSiPR01251. AMPHIPHYSIN.
PR01252. AMPHIPHYSIN1.
PR00452. SH3DOMAIN.
SMARTiSM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADIKTGIFA KNVQKRLNRA QEKVLQKLGK ADETKDEQFE EYVQNFKRQE
60 70 80 90 100
AEGTRLQREL RGYLAAIKGM QEASMKLTES LHEVYEPDWY GREDVKMVGE
110 120 130 140 150
KCDVLWEDFH QKLVDGSLLT LDTYLGQFPD IKNRIAKRSR KLVDYDSARH
160 170 180 190 200
HLEALQSSKR KDESRISKAE EEFQKAQKVF EEFNVDLQEE LPSLWSRRVG
210 220 230 240 250
FYVNTFKNVS SLEAKFHKEI AVLCHKLYEV MTKLGDQHAD KAFSIQGAPS
260 270 280 290 300
DSGPLRIAKT PSPPEEASPL PSPTASPNHT LAPASPAPVR PRSPSQTRKG
310 320 330 340 350
PPVPPLPKVT PTKELQQENI INFFEDNFVP EINVTTPSQN EVLEVKKEET
360 370 380 390 400
LLDLDFDPFK PDVTPAGSAA ATHSPMSQTL PWDLWTTSTD LVQPASGGSF
410 420 430 440 450
NDFTQPQDTS LFTMQTDQNM AETEQALPTE PQAEEPPTTA AAPTAGLDLG
460 470 480 490 500
LEMEEPKEEA AIPPGTDAGE TVGTEGSTGE EAEAEKAALP AGEGESPEGA
510 520 530 540 550
KIDVESTELA SSESPQAAEL EAGAPQEKVI PSVVIEPASN HEGEEHQETT
560 570 580 590 600
TGTETREATE DVAPQGPAGE KQELATEPTP LDSQAATPAP AGAVDASLSA
610 620 630 640 650
GDAAQELPPG FLYKVETLHD FEAANSDELT LQRGDVVLVV PSDSEADQDA
660 670 680
GWLVGVKESD WLQYRDLATY KGLFPENFTR HLE
Length:683
Mass (Da):74,878
Last modified:July 1, 1997 - v1
Checksum:i7FEA4A9E5A1F6631
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13381 mRNA. Translation: CAA73808.1.
RefSeqiNP_071553.1. NM_022217.1.
UniGeneiRn.44463.

Genome annotation databases

GeneIDi60668.
KEGGirno:60668.
UCSCiRGD:620274. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13381 mRNA. Translation: CAA73808.1.
RefSeqiNP_071553.1. NM_022217.1.
UniGeneiRn.44463.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VJ0X-ray1.60Q324-330[»]
ProteinModelPortaliO08838.
SMRiO08838.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248889. 19 interactors.
DIPiDIP-30977N.
IntActiO08838. 8 interactors.
MINTiMINT-101171.
STRINGi10116.ENSRNOP00000017102.

PTM databases

iPTMnetiO08838.
PhosphoSitePlusiO08838.

Proteomic databases

PaxDbiO08838.
PRIDEiO08838.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi60668.
KEGGirno:60668.
UCSCiRGD:620274. rat.

Organism-specific databases

CTDi273.
RGDi620274. Amph.

Phylogenomic databases

eggNOGiKOG3771. Eukaryota.
ENOG410ZBW1. LUCA.
HOGENOMiHOG000252987.
HOVERGENiHBG004224.
InParanoidiO08838.
KOiK12562.
PhylomeDBiO08838.

Miscellaneous databases

EvolutionaryTraceiO08838.
PROiO08838.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR003017. Amphiphysin_1.
IPR004148. BAR_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF03114. BAR. 1 hit.
[Graphical view]
PRINTSiPR01251. AMPHIPHYSIN.
PR01252. AMPHIPHYSIN1.
PR00452. SH3DOMAIN.
SMARTiSM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPH_RAT
AccessioniPrimary (citable) accession number: O08838
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.