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Protein

Amphiphysin

Gene

Amph

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton (By similarity).By similarity

GO - Molecular functioni

  • phospholipid binding Source: RGD
  • protein C-terminus binding Source: RGD
  • protein heterodimerization activity Source: RGD

GO - Biological processi

  • learning Source: RGD
  • positive regulation of endocytosis Source: RGD
  • positive regulation of GTPase activity Source: RGD
  • synaptic vesicle endocytosis Source: RGD

Names & Taxonomyi

Protein namesi
Recommended name:
Amphiphysin
Gene namesi
Name:Amph
Synonyms:Amph1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620274 Amph

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001929491 – 683AmphiphysinAdd BLAST683

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei252PhosphoserineBy similarity1
Modified residuei260PhosphothreonineBy similarity1
Modified residuei262PhosphoserineBy similarity1
Modified residuei268PhosphoserineBy similarity1
Modified residuei272PhosphoserineBy similarity1
Modified residuei276PhosphoserineBy similarity1
Modified residuei280PhosphothreonineBy similarity1
Modified residuei496PhosphoserineBy similarity1
Modified residuei626PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO08838
PRIDEiO08838

PTM databases

iPTMnetiO08838
PhosphoSitePlusiO08838

Interactioni

Subunit structurei

Heterodimer with BIN1. Binds SH3GLB1 (By similarity). Interacts with REPS1 and SGIP1; may be involved in clathrin-mediated endocytosis (By similarity). Binds AP2A2. Interacts with AP2B1.By similarity4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein C-terminus binding Source: RGD
  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

BioGridi248889, 20 interactors
CORUMiO08838
DIPiDIP-30977N
ELMiO08838
IntActiO08838, 10 interactors
MINTiO08838
STRINGi10116.ENSRNOP00000017102

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VJ0X-ray1.60Q324-330[»]
5M5TX-ray1.70E/F/G/H/I/J349-356[»]
ProteinModelPortaliO08838
SMRiO08838
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08838

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 240BARPROSITE-ProRule annotationAdd BLAST217
Domaini610 – 683SH3PROSITE-ProRule annotationAdd BLAST74

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili10 – 83Sequence analysisAdd BLAST74
Coiled coili144 – 191Sequence analysisAdd BLAST48

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG3771 Eukaryota
ENOG410ZBW1 LUCA
HOGENOMiHOG000252987
HOVERGENiHBG004224
InParanoidiO08838
KOiK12562
PhylomeDBiO08838

Family and domain databases

CDDicd12140 SH3_Amphiphysin_I, 1 hit
Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR003005 Amphiphysin
IPR003017 Amphiphysin_1
IPR035470 Amphiphysin_I_SH3
IPR004148 BAR_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR44669 PTHR44669, 1 hit
PfamiView protein in Pfam
PF03114 BAR, 1 hit
PRINTSiPR01251 AMPHIPHYSIN
PR01252 AMPHIPHYSIN1
PR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00721 BAR, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51021 BAR, 1 hit
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

O08838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADIKTGIFA KNVQKRLNRA QEKVLQKLGK ADETKDEQFE EYVQNFKRQE
60 70 80 90 100
AEGTRLQREL RGYLAAIKGM QEASMKLTES LHEVYEPDWY GREDVKMVGE
110 120 130 140 150
KCDVLWEDFH QKLVDGSLLT LDTYLGQFPD IKNRIAKRSR KLVDYDSARH
160 170 180 190 200
HLEALQSSKR KDESRISKAE EEFQKAQKVF EEFNVDLQEE LPSLWSRRVG
210 220 230 240 250
FYVNTFKNVS SLEAKFHKEI AVLCHKLYEV MTKLGDQHAD KAFSIQGAPS
260 270 280 290 300
DSGPLRIAKT PSPPEEASPL PSPTASPNHT LAPASPAPVR PRSPSQTRKG
310 320 330 340 350
PPVPPLPKVT PTKELQQENI INFFEDNFVP EINVTTPSQN EVLEVKKEET
360 370 380 390 400
LLDLDFDPFK PDVTPAGSAA ATHSPMSQTL PWDLWTTSTD LVQPASGGSF
410 420 430 440 450
NDFTQPQDTS LFTMQTDQNM AETEQALPTE PQAEEPPTTA AAPTAGLDLG
460 470 480 490 500
LEMEEPKEEA AIPPGTDAGE TVGTEGSTGE EAEAEKAALP AGEGESPEGA
510 520 530 540 550
KIDVESTELA SSESPQAAEL EAGAPQEKVI PSVVIEPASN HEGEEHQETT
560 570 580 590 600
TGTETREATE DVAPQGPAGE KQELATEPTP LDSQAATPAP AGAVDASLSA
610 620 630 640 650
GDAAQELPPG FLYKVETLHD FEAANSDELT LQRGDVVLVV PSDSEADQDA
660 670 680
GWLVGVKESD WLQYRDLATY KGLFPENFTR HLE
Length:683
Mass (Da):74,878
Last modified:July 1, 1997 - v1
Checksum:i7FEA4A9E5A1F6631
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13381 mRNA Translation: CAA73808.1
RefSeqiNP_071553.1, NM_022217.1
UniGeneiRn.44463

Genome annotation databases

GeneIDi60668
KEGGirno:60668
UCSCiRGD:620274 rat

Similar proteinsi

Entry informationi

Entry nameiAMPH_RAT
AccessioniPrimary (citable) accession number: O08838
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: July 1, 1997
Last modified: April 25, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health