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Protein

Cell division cycle 5-like protein

Gene

Cdc5l

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi31 – 5424H-T-H motifPROSITE-ProRule annotationAdd
BLAST
DNA bindingi82 – 10423H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • leucine zipper domain binding Source: RGD
  • poly(A) RNA binding Source: Ensembl
  • protein kinase binding Source: RGD
  • protein phosphatase 1 binding Source: RGD
  • transcription corepressor binding Source: RGD
  • WD40-repeat domain binding Source: UniProtKB

GO - Biological processi

  • cellular response to fibroblast growth factor stimulus Source: RGD
  • cellular response to interleukin-2 Source: RGD
  • cellular response to nerve growth factor stimulus Source: RGD
  • cellular response to prolactin Source: RGD
  • cellular response to wortmannin Source: RGD
  • DNA repair Source: UniProtKB-KW
  • G2/M transition of mitotic cell cycle Source: RGD
  • mitotic cell cycle Source: RGD
  • mRNA processing Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: RGD
  • RNA splicing Source: UniProtKB-KW
  • signal transduction involved in DNA damage checkpoint Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair, mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle 5-like protein
Alternative name(s):
Cdc5-like protein
Pombe Cdc5-related protein
Gene namesi
Name:Cdc5l
Synonyms:Pcdc5rp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi70892. Cdc5l.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation2 Publications
  • Nucleus speckle PROSITE-ProRule annotation
  • Cytoplasm 1 Publication

  • Note: May shuttle between cytoplasm and nucleus.1 Publication

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: Ensembl
  • DNA replication factor A complex Source: Ensembl
  • membrane Source: Ensembl
  • nuclear speck Source: RGD
  • nucleolus Source: Ensembl
  • nucleus Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • protein-DNA complex Source: RGD
  • Prp19 complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 802802Cell division cycle 5-like proteinPRO_0000197093Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki219 – 219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei227 – 2271PhosphothreonineBy similarity
Modified residuei303 – 3031PhosphoserineBy similarity
Modified residuei358 – 3581PhosphoserineBy similarity
Modified residuei377 – 3771PhosphothreonineCombined sources
Modified residuei385 – 3851PhosphothreonineCombined sources
Modified residuei396 – 3961PhosphothreonineCombined sources
Modified residuei404 – 4041PhosphothreonineBy similarity
Modified residuei411 – 4111PhosphothreonineBy similarity
Modified residuei415 – 4151PhosphothreonineBy similarity
Modified residuei417 – 4171PhosphoserineBy similarity
Modified residuei424 – 4241PhosphothreonineBy similarity
Modified residuei430 – 4301PhosphothreonineBy similarity
Modified residuei438 – 4381PhosphothreonineBy similarity
Modified residuei442 – 4421PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2 (By similarity). Phosphorylation enhances interaction with PPP1R8.By similarity1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO08837.
PRIDEiO08837.

PTM databases

iPTMnetiO08837.
PhosphoSiteiO08837.

Expressioni

Inductioni

By prolactin, IL-2 and FGF-2 in prolactin-dependent lymphoid cells (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000019975.
ExpressionAtlasiO08837. baseline and differential.
GenevisibleiO08837. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with DAPK3 (PubMed:11884640). Belongs to the spliceosome complex. Part of a spliceosomal 'core' complex consisting of CDC5L, PLRG1, SPF27, CCAP1, CCAP3 and CCAP6. Interacts with PLRG1, Lodestar/TTF2, and NIPP1/PPP1R8 (PubMed:10827081). Identified in the spliceosome C complex. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its C-terminus) directly in the complex with PRPF19 and BCAS2. Interacts (via its C-terminus) directly with PRGL1 (via its WD40 repeat domain); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts with CTNNBL1. Interacts with PRPF19 (via N-terminus) (PubMed:16352598).By similarity3 Publications

GO - Molecular functioni

  • leucine zipper domain binding Source: RGD
  • protein kinase binding Source: RGD
  • protein phosphatase 1 binding Source: RGD
  • transcription corepressor binding Source: RGD
  • WD40-repeat domain binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027264.

Structurei

3D structure databases

ProteinModelPortaliO08837.
SMRiO08837. Positions 10-112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5656HTH myb-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini57 – 10852HTH myb-type 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni200 – 2067Required for interaction with CTNNBL1By similarity
Regioni260 – 606347Interaction with PPP1R8By similarityAdd
BLAST
Regioni501 – 659159Interaction with DAPK3Add
BLAST
Regioni706 – 80095Interaction with PLRG1By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili146 – 19247Sequence analysisAdd
BLAST
Coiled coili676 – 70126Sequence analysisAdd
BLAST
Coiled coili761 – 80141Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi165 – 271107Nuclear localization signalSequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the CEF1 family.Curated
Contains 2 HTH myb-type DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0050. Eukaryota.
COG5147. LUCA.
GeneTreeiENSGT00550000074922.
HOGENOMiHOG000182446.
HOVERGENiHBG052766.
InParanoidiO08837.
KOiK12860.
OMAiKERMAHG.
OrthoDBiEOG091G0IYB.
PhylomeDBiO08837.
TreeFamiTF101061.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR021786. Cdc5p/Cef1.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
[Graphical view]
PfamiPF11831. Myb_Cef. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51294. HTH_MYB. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08837-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW
60 70 80 90 100
YEWLDPSIKK TEWSREEEEK LLHLAKLMPT QWRTIAPIIG RTAAQCLEHY
110 120 130 140 150
EFLLDKTAQR DNEEETTDDP RKLKPGEIDP NPETKPARPD PIDMDEDELE
160 170 180 190 200
MLSEARARLA NTQGKKAKRK AREKQLEEAR RLAALQKRRE LRAAGIEIQK
210 220 230 240 250
KRKKKRGVDY NAEIPFEKKP ALGFYDTSEE NYQALDADFR KLRQQDLDGE
260 270 280 290 300
LRSEKEGRDR KKDKQHLKRK KESDLPSAIL QTSGVSEFTK KRSKLVLPAP
310 320 330 340 350
QISDAELQEV VKVGQASEVA RQTAEESGIT NSASSTLLSE YNVTNNSIAL
360 370 380 390 400
RTPRTPASQD RILQEAQNLM ALTNVDTPLK GGLNTPLHES DFSGVTPQRQ
410 420 430 440 450
VVQTPNTVLS TPFRTPSNGA EGLTPRSGTT PKPVTNATPG RTPLRDKLNI
460 470 480 490 500
NPEDGMADYS DPSYVKQMER ESREHLRLGL LGLPAPKNDF EIVLPENAEK
510 520 530 540 550
ELEEREMDDT YIEDAADVDA RKQAIRDAER VKEMKRMHKA VQKDLPRPSE
560 570 580 590 600
VNETILRPLN VEPPLTDLQK SEELIKKEMI TMLHYDLLHH PYEPSGNKKG
610 620 630 640 650
KNVGFATNNS EHITYLEHSP YEKFSKEDLK KAQDVLVQEM EVVKQGMSHG
660 670 680 690 700
ELSSEAYNQV WEECYSQVLY LPAQSRYTRA NLASKKDRIE SLEKRLEINR
710 720 730 740 750
GHMTTEAKRA AKMEKKMKIL LGGYQSRAMG LLKQLNDLWD QIEQAHLELR
760 770 780 790 800
TFEELKKHED SAIPRRLECL KEDVQRQQER EKELQQRYAD LLMEKETLQA

KF
Length:802
Mass (Da):92,218
Last modified:May 1, 2000 - v2
Checksum:i3795F3D37E10FCC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000578 mRNA. Translation: AAD05365.2.
RefSeqiNP_445979.1. NM_053527.1.
UniGeneiRn.54977.

Genome annotation databases

EnsembliENSRNOT00000027264; ENSRNOP00000027264; ENSRNOG00000019975.
GeneIDi85434.
KEGGirno:85434.
UCSCiRGD:70892. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000578 mRNA. Translation: AAD05365.2.
RefSeqiNP_445979.1. NM_053527.1.
UniGeneiRn.54977.

3D structure databases

ProteinModelPortaliO08837.
SMRiO08837. Positions 10-112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027264.

PTM databases

iPTMnetiO08837.
PhosphoSiteiO08837.

Proteomic databases

PaxDbiO08837.
PRIDEiO08837.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027264; ENSRNOP00000027264; ENSRNOG00000019975.
GeneIDi85434.
KEGGirno:85434.
UCSCiRGD:70892. rat.

Organism-specific databases

CTDi988.
RGDi70892. Cdc5l.

Phylogenomic databases

eggNOGiKOG0050. Eukaryota.
COG5147. LUCA.
GeneTreeiENSGT00550000074922.
HOGENOMiHOG000182446.
HOVERGENiHBG052766.
InParanoidiO08837.
KOiK12860.
OMAiKERMAHG.
OrthoDBiEOG091G0IYB.
PhylomeDBiO08837.
TreeFamiTF101061.

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiO08837.

Gene expression databases

BgeeiENSRNOG00000019975.
ExpressionAtlasiO08837. baseline and differential.
GenevisibleiO08837. RN.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR021786. Cdc5p/Cef1.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
[Graphical view]
PfamiPF11831. Myb_Cef. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51294. HTH_MYB. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDC5L_RAT
AccessioniPrimary (citable) accession number: O08837
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.