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Protein

Polypeptide N-acetylgalactosaminyltransferase 4

Gene

Galnt4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a highest activity toward EA2 peptide substrate and a much lower activity with EPO-T, Muc2, Muc1a, Muc1b.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei175SubstrateBy similarity1
Binding sitei204SubstrateBy similarity1
Metal bindingi227ManganeseBy similarity1
Metal bindingi229ManganeseBy similarity1
Binding sitei334SubstrateBy similarity1
Metal bindingi362ManganeseBy similarity1
Binding sitei370SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandLectin, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-913709 O-linked glycosylation of mucins
UniPathwayiUPA00378

Protein family/group databases

CAZyiCBM13 Carbohydrate-Binding Module Family 13
GT27 Glycosyltransferase Family 27

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 4 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 4
Short name:
GalNAc-T4
Short name:
pp-GaNTase 4
Protein-UDP acetylgalactosaminyltransferase 4
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4
Gene namesi
Name:Galnt4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:894692 Galnt4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini36 – 578LumenalSequence analysisAdd BLAST543

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591091 – 578Polypeptide N-acetylgalactosaminyltransferase 4Add BLAST578

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi124 ↔ 357PROSITE-ProRule annotation
Disulfide bondi348 ↔ 421PROSITE-ProRule annotation
Disulfide bondi457 ↔ 477PROSITE-ProRule annotation
Glycosylationi471N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi503 ↔ 518PROSITE-ProRule annotation
Disulfide bondi547 ↔ 565PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO08832
PaxDbiO08832
PeptideAtlasiO08832
PRIDEiO08832

PTM databases

iPTMnetiO08832
PhosphoSitePlusiO08832

Expressioni

Tissue specificityi

Highly expressed in sublingual gland, stomach, colon, small intestine and cervix. Expressed at intermediate levels in kidney, ovary, lung and uterus. Weakly expressed in spleen, liver, heart and brain. Not expressed in submandibular and parotid glands, skeletal muscle and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000090035
GenevisibleiO08832 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000125315

Structurei

3D structure databases

ProteinModelPortaliO08832
SMRiO08832
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini444 – 577Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 243Catalytic subdomain AAdd BLAST110
Regioni303 – 365Catalytic subdomain BAdd BLAST63

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates (By similarity).By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3736 Eukaryota
ENOG410XPMK LUCA
GeneTreeiENSGT00760000118828
HOGENOMiHOG000038227
HOVERGENiHBG051699
InParanoidiO08832
KOiK00710
OMAiISLHRHI
OrthoDBiEOG091G085O
PhylomeDBiO08832
TreeFamiTF352660

Family and domain databases

CDDicd00161 RICIN, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
IPR035992 Ricin_B-like_lectins
IPR000772 Ricin_B_lectin
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
PF00652 Ricin_B_lectin, 1 hit
SMARTiView protein in SMART
SM00458 RICIN, 1 hit
SUPFAMiSSF50370 SSF50370, 1 hit
SSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS50231 RICIN_B_LECTIN, 1 hit

Sequencei

Sequence statusi: Complete.

O08832-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVRWTWAGK SCLLLALLTL AYILVEFSVS TLYASPGAGG ARELGPRRLP
60 70 80 90 100
DLDTREEDLS QPLYIKPPAD SHALGEWGRA SKLQLNEGEL KQQEELIERY
110 120 130 140 150
AINIYLSDRI SLHRHIEDKR MYECKAKKFH YRSLPTTSVI IAFYNEAWST
160 170 180 190 200
LLRTIHSVLE TSPAVLLKEI ILVDDLSDRI YLKAQLETYI SNLERVRLIR
210 220 230 240 250
TNKREGLVRA RLIGATFATG DVLTFLDCHC ECNTGWLEPL LERISRDETA
260 270 280 290 300
IVCPVIDTID WNTFEFYMQT GEPMIGGFDW RLTFQWHSVP KHERDRRTSR
310 320 330 340 350
IDPIRSPTMA GGLFAVSKKY FQYLGTYDTG MEVWGGENLE LSFRVWQCGG
360 370 380 390 400
KLEIHPCSHV GHVFPKRAPY ARPNFLQNTA RAAEVWMDEY KEHFYNRNPP
410 420 430 440 450
ARKEAYGDLS ERKLLRERLK CKSFDWYLKN VFSNLHVPED RPGWHGAIRS
460 470 480 490 500
MGISSECLDY NAPDNNPTGA NLSLFGCHGQ GGNQFFEYTS NKEIRFNSVT
510 520 530 540 550
ELCAEVPQQK DYVGMQNCPK DGLPVPVNII WHFKEDGTIF HPHTRLCLSA
560 570
YRTAEGRPSV HMKTCDALDK NQLWRFEK
Length:578
Mass (Da):66,555
Last modified:July 1, 1997 - v1
Checksum:i10ADC0D8B8B30835
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73819 mRNA Translation: AAB58301.1
AK033494 mRNA Translation: BAC28317.1
AK148036 mRNA Translation: BAE28303.1
AK153253 mRNA Translation: BAE31844.1
BC057882 mRNA Translation: AAH57882.1
CCDSiCCDS48679.1
RefSeqiNP_056552.1, NM_015737.4
UniGeneiMm.314

Genome annotation databases

EnsembliENSMUST00000161240; ENSMUSP00000125315; ENSMUSG00000090035
GeneIDi14426
KEGGimmu:14426
UCSCiuc007gxj.2 mouse

Similar proteinsi

Entry informationi

Entry nameiGALT4_MOUSE
AccessioniPrimary (citable) accession number: O08832
Secondary accession number(s): Q3U681
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 1, 1997
Last modified: March 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health