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O08816

- WASL_RAT

UniProt

O08816 - WASL_RAT

Protein

Neural Wiskott-Aldrich syndrome protein

Gene

Wasl

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression By similarity.By similarity

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. actin filament organization Source: InterPro
    2. membrane budding Source: BHF-UCL
    3. membrane invagination Source: BHF-UCL
    4. membrane tubulation Source: BHF-UCL
    5. mitotic nuclear division Source: UniProtKB-KW
    6. positive regulation of clathrin-mediated endocytosis Source: BHF-UCL
    7. positive regulation of filopodium assembly Source: BHF-UCL
    8. regulation of transcription, DNA-templated Source: UniProtKB-KW
    9. transcription, DNA-templated Source: UniProtKB-KW
    10. vesicle organization Source: BHF-UCL
    11. vesicle transport along actin filament Source: BHF-UCL

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

    Keywords - Ligandi

    Actin-binding

    Enzyme and pathway databases

    ReactomeiREACT_196755. Regulation of actin dynamics for phagocytic cup formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neural Wiskott-Aldrich syndrome protein
    Short name:
    N-WASP
    Gene namesi
    Name:Wasl
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi735144. Wasl.

    Subcellular locationi

    Cytoplasmcytoskeleton 1 Publication. Nucleus 1 Publication
    Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated.By similarity

    GO - Cellular componenti

    1. cytoplasmic membrane-bounded vesicle Source: BHF-UCL
    2. cytoskeleton Source: UniProtKB-SubCell
    3. cytosol Source: Reactome
    4. Golgi membrane Source: RGD
    5. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 501500Neural Wiskott-Aldrich syndrome proteinPRO_0000189002Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei239 – 2391Phosphoserine; by TNK2By similarity
    Modified residuei253 – 2531Phosphotyrosine; by FAK1 and TNK21 Publication
    Modified residuei480 – 4801PhosphoserineBy similarity
    Modified residuei481 – 4811PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    PTM databases

    PhosphoSiteiO08816.

    Expressioni

    Gene expression databases

    GenevestigatoriO08816.

    Interactioni

    Subunit structurei

    Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11. Interacts with NOSTRIN, SNX9 and SNX33. Binds to TNK2. Interacts with PACSIN1, PACSIN2 and PACSIN3 By similarity. Interacts with PTK2/FAK1.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-6142604,EBI-6142604
    CDC42P609532EBI-6142604,EBI-81752From a different organism.
    NOSTRINQ8IVI93EBI-6142604,EBI-1391643From a different organism.
    PFN1P077374EBI-6142604,EBI-713780From a different organism.
    PFN2P350803EBI-6142604,EBI-473138From a different organism.
    Wipf1Q6IN362EBI-6142604,EBI-6986245

    Protein-protein interaction databases

    DIPiDIP-17016N.
    IntActiO08816. 12 interactions.
    MINTiMINT-250197.
    STRINGi10116.ENSRNOP00000009261.

    Structurei

    Secondary structure

    1
    501
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi36 – 4914
    Turni50 – 523
    Beta strandi53 – 6715
    Turni68 – 714
    Beta strandi72 – 787
    Beta strandi80 – 823
    Beta strandi84 – 907
    Beta strandi100 – 1089
    Beta strandi110 – 12011
    Helixi121 – 13717

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MKENMR-A26-147[»]
    2IFSNMR-A26-147[»]
    ProteinModelPortaliO08816.
    SMRiO08816. Positions 26-147, 201-272.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO08816.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 138108WH1PROSITE-ProRule annotationAdd
    BLAST
    Domaini200 – 21314CRIBPROSITE-ProRule annotationAdd
    BLAST
    Domaini401 – 41818WH2 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini429 – 44618WH2 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi274 – 385112Pro-richAdd
    BLAST
    Compositional biasi482 – 50120Asp-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CRIB domain.PROSITE-ProRule annotation
    Contains 1 WH1 domain.PROSITE-ProRule annotation
    Contains 2 WH2 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG270974.
    HOGENOMiHOG000214052.
    HOVERGENiHBG000222.
    InParanoidiO08816.
    PhylomeDBiO08816.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    3.90.810.10. 1 hit.
    InterProiIPR000095. CRIB_dom.
    IPR011993. PH_like_dom.
    IPR011026. WASP_C.
    IPR000697. WH1/EVH1.
    IPR003124. WH2_dom.
    [Graphical view]
    PfamiPF00786. PBD. 1 hit.
    PF00568. WH1. 1 hit.
    PF02205. WH2. 2 hits.
    [Graphical view]
    SMARTiSM00285. PBD. 1 hit.
    SM00461. WH1. 1 hit.
    SM00246. WH2. 2 hits.
    [Graphical view]
    SUPFAMiSSF47912. SSF47912. 2 hits.
    PROSITEiPS50108. CRIB. 1 hit.
    PS50229. WH1. 1 hit.
    PS51082. WH2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O08816-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSGQQPPRR VTNVGSLLLT PQENESLFSF LGKKCVTMSS AVVQLYAADR    50
    NCMWSKKCSG VACLVKDNPQ RSYFLRIFDI KDGKLLWEQE LYNNFVYNSP 100
    RGYFHTFAGD TCQVALNFAN EEEAKKFRKA VTDLLGRRQR KSEKRRDAPN 150
    GPNLPMATVD IKNPEITTNR FYSSQVNNIS HTKEKKKGKA KKKRLTKADI 200
    GTPSNFQHIG HVGWDPNTGF DLNNLDPELK NLFDMCGISE AQLKDRETSK 250
    VIYDFIEKTG GVEAVKNELR RQAPPPPPPS RGGPPPPPPP PHSSGPPPPP 300
    ARGRGAPPPP PSRAPTAAPP PPPPSRPGVV VPPPPPNRMY PPPPPALPSS 350
    APSGPPPPPP LSMAGSTAPP PPPPPPPPPG PPPPPGLPSD GDHQVPASSG 400
    NKAALLDQIR EGAQLKKVEQ NSRPVSCSGR DALLDQIRQG IQLKSVSDGQ 450
    ESTPPTPAPT SGIVGALMEV MQKRSKAIHS SDEDEDDDDE EDFQDDDEWE 500
    D 501
    Length:501
    Mass (Da):54,325
    Last modified:January 1, 1998 - v2
    Checksum:i480E21F26F7FC77E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D88461 mRNA. Translation: BAA21534.1.
    UniGeneiRn.126640.

    Genome annotation databases

    UCSCiRGD:735144. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D88461 mRNA. Translation: BAA21534.1 .
    UniGenei Rn.126640.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MKE NMR - A 26-147 [» ]
    2IFS NMR - A 26-147 [» ]
    ProteinModelPortali O08816.
    SMRi O08816. Positions 26-147, 201-272.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-17016N.
    IntActi O08816. 12 interactions.
    MINTi MINT-250197.
    STRINGi 10116.ENSRNOP00000009261.

    PTM databases

    PhosphoSitei O08816.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:735144. rat.

    Organism-specific databases

    RGDi 735144. Wasl.

    Phylogenomic databases

    eggNOGi NOG270974.
    HOGENOMi HOG000214052.
    HOVERGENi HBG000222.
    InParanoidi O08816.
    PhylomeDBi O08816.

    Enzyme and pathway databases

    Reactomei REACT_196755. Regulation of actin dynamics for phagocytic cup formation.

    Miscellaneous databases

    EvolutionaryTracei O08816.

    Gene expression databases

    Genevestigatori O08816.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    3.90.810.10. 1 hit.
    InterProi IPR000095. CRIB_dom.
    IPR011993. PH_like_dom.
    IPR011026. WASP_C.
    IPR000697. WH1/EVH1.
    IPR003124. WH2_dom.
    [Graphical view ]
    Pfami PF00786. PBD. 1 hit.
    PF00568. WH1. 1 hit.
    PF02205. WH2. 2 hits.
    [Graphical view ]
    SMARTi SM00285. PBD. 1 hit.
    SM00461. WH1. 1 hit.
    SM00246. WH2. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47912. SSF47912. 2 hits.
    PROSITEi PS50108. CRIB. 1 hit.
    PS50229. WH1. 1 hit.
    PS51082. WH2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of N-WASP homologs in human and rat brain."
      Fukuoka M., Miki H., Takenawa T.
      Gene 196:43-48(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Focal adhesion kinase regulation of N-WASP subcellular localization and function."
      Wu X., Suetsugu S., Cooper L.A., Takenawa T., Guan J.L.
      J. Biol. Chem. 279:9565-9576(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PTK2/FAK1, SUBCELLULAR LOCATION, PHOSPHORYLATION AT TYR-253.
    3. "Structure of the N-WASP EVH1 domain-WIP complex: insight into the molecular basis of Wiskott-Aldrich Syndrome."
      Volkman B.F., Prehoda K.E., Scott J.A., Peterson F.C., Lim W.A.
      Cell 111:565-576(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 37-485.

    Entry informationi

    Entry nameiWASL_RAT
    AccessioniPrimary (citable) accession number: O08816
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3