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Protein

Neural Wiskott-Aldrich syndrome protein

Gene

Wasl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (By similarity).By similarity

GO - Molecular functioni

  • cytoskeletal protein binding Source: RGD

GO - Biological processi

  • actin filament organization Source: InterPro
  • cell division Source: UniProtKB-KW
  • membrane budding Source: BHF-UCL
  • membrane invagination Source: BHF-UCL
  • membrane tubulation Source: BHF-UCL
  • mitotic nuclear division Source: UniProtKB-KW
  • positive regulation of Arp2/3 complex-mediated actin nucleation Source: InterPro
  • positive regulation of clathrin-dependent endocytosis Source: BHF-UCL
  • positive regulation of filopodium assembly Source: BHF-UCL
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
  • vesicle organization Source: BHF-UCL
  • vesicle transport along actin filament Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neural Wiskott-Aldrich syndrome protein
Short name:
N-WASP
Gene namesi
Name:Wasl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi735144. Wasl.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm By similarity
  • Cytoplasmcytoskeleton 1 Publication

  • Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated. Exported from the nucleus by an nuclear export signal (NES)-dependent mechanism to the cytoplasm.By similarity

GO - Cellular componenti

  • cell leading edge Source: RGD
  • cytoplasmic, membrane-bounded vesicle Source: BHF-UCL
  • cytoskeleton Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • Golgi membrane Source: RGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001890022 – 501Neural Wiskott-Aldrich syndrome proteinAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei239Phosphoserine; by TNK2By similarity1
Modified residuei253Phosphotyrosine; by FAK1 and TNK2Combined sources1 Publication1
Modified residuei304Omega-N-methylarginineBy similarity1
Modified residuei480PhosphoserineBy similarity1
Modified residuei481PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiO08816.
PRIDEiO08816.

PTM databases

iPTMnetiO08816.

Interactioni

Subunit structurei

Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11. Interacts with NOSTRIN, SNX9 and SNX33. Binds to TNK2. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Interacts with PTK2/FAK1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-6142604,EBI-6142604
CDC42P609532EBI-6142604,EBI-81752From a different organism.
NOSTRINQ8IVI93EBI-6142604,EBI-1391643From a different organism.
PFN1P077374EBI-6142604,EBI-713780From a different organism.
PFN2P350803EBI-6142604,EBI-473138From a different organism.
Wipf1Q6IN362EBI-6142604,EBI-6986245

GO - Molecular functioni

  • cytoskeletal protein binding Source: RGD

Protein-protein interaction databases

DIPiDIP-17016N.
IntActiO08816. 12 interactors.
MINTiMINT-250197.
STRINGi10116.ENSRNOP00000009261.

Structurei

Secondary structure

1501
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi36 – 49Combined sources14
Turni50 – 52Combined sources3
Beta strandi53 – 67Combined sources15
Turni68 – 71Combined sources4
Beta strandi72 – 78Combined sources7
Beta strandi80 – 82Combined sources3
Beta strandi84 – 90Combined sources7
Beta strandi100 – 108Combined sources9
Beta strandi110 – 120Combined sources11
Helixi121 – 137Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MKENMR-A26-147[»]
2IFSNMR-A26-147[»]
ProteinModelPortaliO08816.
SMRiO08816.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08816.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 138WH1PROSITE-ProRule annotationAdd BLAST108
Domaini200 – 213CRIBPROSITE-ProRule annotationAdd BLAST14
Domaini401 – 418WH2 1PROSITE-ProRule annotationAdd BLAST18
Domaini429 – 446WH2 2PROSITE-ProRule annotationAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi274 – 385Pro-richAdd BLAST112
Compositional biasi482 – 501Asp-richAdd BLAST20

Sequence similaritiesi

Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 WH1 domain.PROSITE-ProRule annotation
Contains 2 WH2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3671. Eukaryota.
ENOG4111H4S. LUCA.
HOGENOMiHOG000214052.
HOVERGENiHBG000222.
PhylomeDBiO08816.

Family and domain databases

CDDicd01205. EVH1_WASP-like. 1 hit.
Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR030214. N-WASP.
IPR011993. PH_dom-like.
IPR011026. WASP_C.
IPR033927. WASPfam_EVH1.
IPR000697. WH1/EVH1_dom.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23202:SF33. PTHR23202:SF33. 1 hit.
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGQQPPRR VTNVGSLLLT PQENESLFSF LGKKCVTMSS AVVQLYAADR
60 70 80 90 100
NCMWSKKCSG VACLVKDNPQ RSYFLRIFDI KDGKLLWEQE LYNNFVYNSP
110 120 130 140 150
RGYFHTFAGD TCQVALNFAN EEEAKKFRKA VTDLLGRRQR KSEKRRDAPN
160 170 180 190 200
GPNLPMATVD IKNPEITTNR FYSSQVNNIS HTKEKKKGKA KKKRLTKADI
210 220 230 240 250
GTPSNFQHIG HVGWDPNTGF DLNNLDPELK NLFDMCGISE AQLKDRETSK
260 270 280 290 300
VIYDFIEKTG GVEAVKNELR RQAPPPPPPS RGGPPPPPPP PHSSGPPPPP
310 320 330 340 350
ARGRGAPPPP PSRAPTAAPP PPPPSRPGVV VPPPPPNRMY PPPPPALPSS
360 370 380 390 400
APSGPPPPPP LSMAGSTAPP PPPPPPPPPG PPPPPGLPSD GDHQVPASSG
410 420 430 440 450
NKAALLDQIR EGAQLKKVEQ NSRPVSCSGR DALLDQIRQG IQLKSVSDGQ
460 470 480 490 500
ESTPPTPAPT SGIVGALMEV MQKRSKAIHS SDEDEDDDDE EDFQDDDEWE

D
Length:501
Mass (Da):54,325
Last modified:January 1, 1998 - v2
Checksum:i480E21F26F7FC77E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88461 mRNA. Translation: BAA21534.1.
UniGeneiRn.126640.

Genome annotation databases

UCSCiRGD:735144. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88461 mRNA. Translation: BAA21534.1.
UniGeneiRn.126640.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MKENMR-A26-147[»]
2IFSNMR-A26-147[»]
ProteinModelPortaliO08816.
SMRiO08816.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-17016N.
IntActiO08816. 12 interactors.
MINTiMINT-250197.
STRINGi10116.ENSRNOP00000009261.

PTM databases

iPTMnetiO08816.

Proteomic databases

PaxDbiO08816.
PRIDEiO08816.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:735144. rat.

Organism-specific databases

RGDi735144. Wasl.

Phylogenomic databases

eggNOGiKOG3671. Eukaryota.
ENOG4111H4S. LUCA.
HOGENOMiHOG000214052.
HOVERGENiHBG000222.
PhylomeDBiO08816.

Miscellaneous databases

EvolutionaryTraceiO08816.
PROiO08816.

Family and domain databases

CDDicd01205. EVH1_WASP-like. 1 hit.
Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR030214. N-WASP.
IPR011993. PH_dom-like.
IPR011026. WASP_C.
IPR033927. WASPfam_EVH1.
IPR000697. WH1/EVH1_dom.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23202:SF33. PTHR23202:SF33. 1 hit.
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWASL_RAT
AccessioniPrimary (citable) accession number: O08816
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.