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O08816

- WASL_RAT

UniProt

O08816 - WASL_RAT

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Protein

Neural Wiskott-Aldrich syndrome protein

Gene

Wasl

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (By similarity).By similarity

GO - Molecular functioni

  1. identical protein binding Source: IntAct

GO - Biological processi

  1. actin filament organization Source: InterPro
  2. membrane budding Source: BHF-UCL
  3. membrane invagination Source: BHF-UCL
  4. membrane tubulation Source: BHF-UCL
  5. mitotic nuclear division Source: UniProtKB-KW
  6. positive regulation of clathrin-mediated endocytosis Source: BHF-UCL
  7. positive regulation of filopodium assembly Source: BHF-UCL
  8. regulation of transcription, DNA-templated Source: UniProtKB-KW
  9. transcription, DNA-templated Source: UniProtKB-KW
  10. vesicle organization Source: BHF-UCL
  11. vesicle transport along actin filament Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiREACT_196755. Regulation of actin dynamics for phagocytic cup formation.

Names & Taxonomyi

Protein namesi
Recommended name:
Neural Wiskott-Aldrich syndrome protein
Short name:
N-WASP
Gene namesi
Name:Wasl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi735144. Wasl.

Subcellular locationi

Cytoplasmcytoskeleton 1 Publication. Nucleus 1 Publication
Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated.By similarity

GO - Cellular componenti

  1. cytoplasmic membrane-bounded vesicle Source: BHF-UCL
  2. cytoskeleton Source: UniProtKB-KW
  3. cytosol Source: Reactome
  4. Golgi membrane Source: RGD
  5. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 501500Neural Wiskott-Aldrich syndrome proteinPRO_0000189002Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei239 – 2391Phosphoserine; by TNK2By similarity
Modified residuei253 – 2531Phosphotyrosine; by FAK1 and TNK21 Publication
Modified residuei480 – 4801PhosphoserineBy similarity
Modified residuei481 – 4811PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

PTM databases

PhosphoSiteiO08816.

Expressioni

Gene expression databases

GenevestigatoriO08816.

Interactioni

Subunit structurei

Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11. Interacts with NOSTRIN, SNX9 and SNX33. Binds to TNK2. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Interacts with PTK2/FAK1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-6142604,EBI-6142604
CDC42P609532EBI-6142604,EBI-81752From a different organism.
NOSTRINQ8IVI93EBI-6142604,EBI-1391643From a different organism.
PFN1P077374EBI-6142604,EBI-713780From a different organism.
PFN2P350803EBI-6142604,EBI-473138From a different organism.
Wipf1Q6IN362EBI-6142604,EBI-6986245

Protein-protein interaction databases

DIPiDIP-17016N.
IntActiO08816. 12 interactions.
MINTiMINT-250197.
STRINGi10116.ENSRNOP00000009261.

Structurei

Secondary structure

1
501
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi36 – 4914Combined sources
Turni50 – 523Combined sources
Beta strandi53 – 6715Combined sources
Turni68 – 714Combined sources
Beta strandi72 – 787Combined sources
Beta strandi80 – 823Combined sources
Beta strandi84 – 907Combined sources
Beta strandi100 – 1089Combined sources
Beta strandi110 – 12011Combined sources
Helixi121 – 13717Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MKENMR-A26-147[»]
2IFSNMR-A26-147[»]
ProteinModelPortaliO08816.
SMRiO08816. Positions 26-147, 201-272.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08816.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 138108WH1PROSITE-ProRule annotationAdd
BLAST
Domaini200 – 21314CRIBPROSITE-ProRule annotationAdd
BLAST
Domaini401 – 41818WH2 1PROSITE-ProRule annotationAdd
BLAST
Domaini429 – 44618WH2 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi274 – 385112Pro-richAdd
BLAST
Compositional biasi482 – 50120Asp-richAdd
BLAST

Sequence similaritiesi

Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 WH1 domain.PROSITE-ProRule annotation
Contains 2 WH2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG270974.
HOGENOMiHOG000214052.
HOVERGENiHBG000222.
PhylomeDBiO08816.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR011993. PH_like_dom.
IPR011026. WASP_C.
IPR000697. WH1/EVH1.
IPR003124. WH2_dom.
[Graphical view]
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08816-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSGQQPPRR VTNVGSLLLT PQENESLFSF LGKKCVTMSS AVVQLYAADR
60 70 80 90 100
NCMWSKKCSG VACLVKDNPQ RSYFLRIFDI KDGKLLWEQE LYNNFVYNSP
110 120 130 140 150
RGYFHTFAGD TCQVALNFAN EEEAKKFRKA VTDLLGRRQR KSEKRRDAPN
160 170 180 190 200
GPNLPMATVD IKNPEITTNR FYSSQVNNIS HTKEKKKGKA KKKRLTKADI
210 220 230 240 250
GTPSNFQHIG HVGWDPNTGF DLNNLDPELK NLFDMCGISE AQLKDRETSK
260 270 280 290 300
VIYDFIEKTG GVEAVKNELR RQAPPPPPPS RGGPPPPPPP PHSSGPPPPP
310 320 330 340 350
ARGRGAPPPP PSRAPTAAPP PPPPSRPGVV VPPPPPNRMY PPPPPALPSS
360 370 380 390 400
APSGPPPPPP LSMAGSTAPP PPPPPPPPPG PPPPPGLPSD GDHQVPASSG
410 420 430 440 450
NKAALLDQIR EGAQLKKVEQ NSRPVSCSGR DALLDQIRQG IQLKSVSDGQ
460 470 480 490 500
ESTPPTPAPT SGIVGALMEV MQKRSKAIHS SDEDEDDDDE EDFQDDDEWE

D
Length:501
Mass (Da):54,325
Last modified:January 1, 1998 - v2
Checksum:i480E21F26F7FC77E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D88461 mRNA. Translation: BAA21534.1.
UniGeneiRn.126640.

Genome annotation databases

UCSCiRGD:735144. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D88461 mRNA. Translation: BAA21534.1 .
UniGenei Rn.126640.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MKE NMR - A 26-147 [» ]
2IFS NMR - A 26-147 [» ]
ProteinModelPortali O08816.
SMRi O08816. Positions 26-147, 201-272.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-17016N.
IntActi O08816. 12 interactions.
MINTi MINT-250197.
STRINGi 10116.ENSRNOP00000009261.

PTM databases

PhosphoSitei O08816.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:735144. rat.

Organism-specific databases

RGDi 735144. Wasl.

Phylogenomic databases

eggNOGi NOG270974.
HOGENOMi HOG000214052.
HOVERGENi HBG000222.
PhylomeDBi O08816.

Enzyme and pathway databases

Reactomei REACT_196755. Regulation of actin dynamics for phagocytic cup formation.

Miscellaneous databases

EvolutionaryTracei O08816.

Gene expression databases

Genevestigatori O08816.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProi IPR000095. CRIB_dom.
IPR011993. PH_like_dom.
IPR011026. WASP_C.
IPR000697. WH1/EVH1.
IPR003124. WH2_dom.
[Graphical view ]
Pfami PF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view ]
SMARTi SM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view ]
SUPFAMi SSF47912. SSF47912. 2 hits.
PROSITEi PS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of N-WASP homologs in human and rat brain."
    Fukuoka M., Miki H., Takenawa T.
    Gene 196:43-48(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Focal adhesion kinase regulation of N-WASP subcellular localization and function."
    Wu X., Suetsugu S., Cooper L.A., Takenawa T., Guan J.L.
    J. Biol. Chem. 279:9565-9576(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTK2/FAK1, SUBCELLULAR LOCATION, PHOSPHORYLATION AT TYR-253.
  3. "Structure of the N-WASP EVH1 domain-WIP complex: insight into the molecular basis of Wiskott-Aldrich Syndrome."
    Volkman B.F., Prehoda K.E., Scott J.A., Peterson F.C., Lim W.A.
    Cell 111:565-576(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 37-485.

Entry informationi

Entry nameiWASL_RAT
AccessioniPrimary (citable) accession number: O08816
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3