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O08816 (WASL_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neural Wiskott-Aldrich syndrome protein

Short name=N-WASP
Gene names
Name:Wasl
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression By similarity.

Subunit structure

Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11. Interacts with NOSTRIN, SNX9 and SNX33. Binds to TNK2. Interacts with PACSIN1, PACSIN2 and PACSIN3 By similarity. Interacts with PTK2/FAK1. Ref.2

Subcellular location

Cytoplasmcytoskeleton. Nucleus. Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated By similarity. Ref.2

Sequence similarities

Contains 1 CRIB domain.

Contains 1 WH1 domain.

Contains 2 WH2 domains.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
Transcription
Transcription regulation
   Cellular componentCytoplasm
Cytoskeleton
Nucleus
   DomainRepeat
   LigandActin-binding
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin filament organization

Inferred from electronic annotation. Source: InterPro

membrane budding

Inferred from direct assay PubMed 20730103. Source: BHF-UCL

membrane invagination

Inferred from direct assay PubMed 20730103. Source: BHF-UCL

membrane tubulation

Inferred from direct assay PubMed 20730103. Source: BHF-UCL

mitosis

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of Arp2/3 complex-mediated actin nucleation

Inferred from electronic annotation. Source: InterPro

positive regulation of clathrin-mediated endocytosis

Inferred from direct assay PubMed 20730103. Source: BHF-UCL

positive regulation of filopodium assembly

Inferred from direct assay PubMed 20730103. Source: BHF-UCL

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

vesicle organization

Inferred from direct assay PubMed 20730103. Source: BHF-UCL

vesicle transport along actin filament

Inferred from direct assay PubMed 20730103. Source: BHF-UCL

   Cellular_componentGolgi membrane

Inferred from direct assay PubMed 15147909. Source: RGD

cytoplasmic membrane-bounded vesicle

Inferred from direct assay PubMed 20730103. Source: BHF-UCL

cytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytosol

Traceable author statement. Source: Reactome

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionidentical protein binding

Inferred from physical interaction PubMed 11052943PubMed 9422512. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-6142604,EBI-6142604
CDC42P609532EBI-6142604,EBI-81752From a different organism.
NOSTRINQ8IVI93EBI-6142604,EBI-1391643From a different organism.
PFN1P077374EBI-6142604,EBI-713780From a different organism.
PFN2P350803EBI-6142604,EBI-473138From a different organism.
Wipf1Q6IN362EBI-6142604,EBI-6986245

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 501500Neural Wiskott-Aldrich syndrome protein
PRO_0000189002

Regions

Domain31 – 138108WH1
Domain200 – 21314CRIB
Domain401 – 41818WH2 1
Domain429 – 44618WH2 2
Compositional bias274 – 385112Pro-rich
Compositional bias482 – 50120Asp-rich

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue2391Phosphoserine; by TNK2 By similarity
Modified residue2531Phosphotyrosine; by FAK1 and TNK2 Ref.2
Modified residue4801Phosphoserine By similarity
Modified residue4811Phosphoserine By similarity

Secondary structure

................ 501
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O08816 [UniParc].

Last modified January 1, 1998. Version 2.
Checksum: 480E21F26F7FC77E

FASTA50154,325
        10         20         30         40         50         60 
MSSGQQPPRR VTNVGSLLLT PQENESLFSF LGKKCVTMSS AVVQLYAADR NCMWSKKCSG 

        70         80         90        100        110        120 
VACLVKDNPQ RSYFLRIFDI KDGKLLWEQE LYNNFVYNSP RGYFHTFAGD TCQVALNFAN 

       130        140        150        160        170        180 
EEEAKKFRKA VTDLLGRRQR KSEKRRDAPN GPNLPMATVD IKNPEITTNR FYSSQVNNIS 

       190        200        210        220        230        240 
HTKEKKKGKA KKKRLTKADI GTPSNFQHIG HVGWDPNTGF DLNNLDPELK NLFDMCGISE 

       250        260        270        280        290        300 
AQLKDRETSK VIYDFIEKTG GVEAVKNELR RQAPPPPPPS RGGPPPPPPP PHSSGPPPPP 

       310        320        330        340        350        360 
ARGRGAPPPP PSRAPTAAPP PPPPSRPGVV VPPPPPNRMY PPPPPALPSS APSGPPPPPP 

       370        380        390        400        410        420 
LSMAGSTAPP PPPPPPPPPG PPPPPGLPSD GDHQVPASSG NKAALLDQIR EGAQLKKVEQ 

       430        440        450        460        470        480 
NSRPVSCSGR DALLDQIRQG IQLKSVSDGQ ESTPPTPAPT SGIVGALMEV MQKRSKAIHS 

       490        500 
SDEDEDDDDE EDFQDDDEWE D 

« Hide

References

[1]"Identification of N-WASP homologs in human and rat brain."
Fukuoka M., Miki H., Takenawa T.
Gene 196:43-48(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Focal adhesion kinase regulation of N-WASP subcellular localization and function."
Wu X., Suetsugu S., Cooper L.A., Takenawa T., Guan J.L.
J. Biol. Chem. 279:9565-9576(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PTK2/FAK1, SUBCELLULAR LOCATION, PHOSPHORYLATION AT TYR-253.
[3]"Structure of the N-WASP EVH1 domain-WIP complex: insight into the molecular basis of Wiskott-Aldrich Syndrome."
Volkman B.F., Prehoda K.E., Scott J.A., Peterson F.C., Lim W.A.
Cell 111:565-576(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 37-485.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D88461 mRNA. Translation: BAA21534.1.
UniGeneRn.126640.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1MKENMR-A26-147[»]
2IFSNMR-A26-147[»]
ProteinModelPortalO08816.
SMRO08816. Positions 26-147, 201-272.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-17016N.
IntActO08816. 12 interactions.
MINTMINT-250197.
STRING10116.ENSRNOP00000009261.

PTM databases

PhosphoSiteO08816.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:735144. rat.

Organism-specific databases

RGD735144. Wasl.

Phylogenomic databases

eggNOGNOG270974.
HOGENOMHOG000214052.
HOVERGENHBG000222.
InParanoidO08816.
PhylomeDBO08816.

Gene expression databases

GenevestigatorO08816.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProIPR000095. CRIB_dom.
IPR011993. PH_like_dom.
IPR027641. WASP.
IPR011026. WASP_C.
IPR000697. WH1/EVH1.
IPR003124. WH2_dom.
[Graphical view]
PANTHERPTHR12779. PTHR12779. 1 hit.
PfamPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMSSF47912. SSF47912. 2 hits.
PROSITEPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO08816.

Entry information

Entry nameWASL_RAT
AccessionPrimary (citable) accession number: O08816
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references