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Protein

STE20-like serine/threonine-protein kinase

Gene

Slk

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates apoptosis and actin stress fiber dissolution.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631ATPPROSITE-ProRule annotation
Active sitei155 – 1551Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 489ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cellular response to tumor necrosis factor Source: RGD
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of histone phosphorylation Source: RGD
  • protein autophosphorylation Source: RGD
  • protein phosphorylation Source: RGD
  • regulation of cell migration Source: UniProtKB
  • regulation of focal adhesion assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
STE20-like serine/threonine-protein kinase (EC:2.7.11.1)
Short name:
STE20-like kinase
Alternative name(s):
STE20-related serine/threonine-protein kinase
Short name:
STE20-related kinase
Gene namesi
Name:Slk
Synonyms:Sk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3780. Slk.

Subcellular locationi

GO - Cellular componenti

  • cell leading edge Source: UniProtKB
  • cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12061206STE20-like serine/threonine-protein kinasePRO_0000233241Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei183 – 1831PhosphothreonineBy similarity
Modified residuei189 – 1891PhosphoserineBy similarity
Modified residuei340 – 3401PhosphoserineCombined sources
Modified residuei344 – 3441PhosphoserineBy similarity
Modified residuei347 – 3471PhosphoserineCombined sources
Modified residuei348 – 3481PhosphoserineCombined sources
Modified residuei354 – 3541PhosphoserineBy similarity
Modified residuei372 – 3721PhosphoserineBy similarity
Modified residuei545 – 5451PhosphoserineBy similarity
Modified residuei563 – 5631PhosphoserineBy similarity
Modified residuei645 – 6451PhosphoserineCombined sources
Modified residuei649 – 6491PhosphoserineCombined sources
Modified residuei668 – 6681PhosphoserineBy similarity
Modified residuei776 – 7761PhosphoserineBy similarity
Modified residuei778 – 7781PhosphoserineCombined sources
Modified residuei813 – 8131PhosphothreonineBy similarity
Modified residuei817 – 8171PhosphoserineBy similarity
Modified residuei1065 – 10651PhosphothreonineBy similarity

Post-translational modificationi

Proteolytically cleaved by caspase-3.By similarity
Autophosphorylated.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei438 – 4392Cleavage; by caspase-3By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO08815.
PRIDEiO08815.

PTM databases

iPTMnetiO08815.
PhosphoSiteiO08815.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiO08815. 1 interaction.
STRINGi10116.ENSRNOP00000015496.

Structurei

3D structure databases

ProteinModelPortaliO08815.
SMRiO08815. Positions 21-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 292259Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini874 – 90936UVRPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili468 – 49225Sequence analysisAdd
BLAST
Coiled coili825 – 1037213Sequence analysisAdd
BLAST
Coiled coili1077 – 115175Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi16 – 194Poly-Lys
Compositional biasi322 – 3254Poly-Asp

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 UVR domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0579. Eukaryota.
ENOG410XPQN. LUCA.
HOGENOMiHOG000236268.
HOVERGENiHBG052712.
InParanoidiO08815.
KOiK08836.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR022165. PKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR001943. UVR_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF12474. PKK. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08815-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFFNFRKIF KLGSEKKKKQ YEHVKRDLNP EEFWEIIGEL GDGAFGKVYK
60 70 80 90 100
AQNKETNVLA AAKVIDTKSE EELEDYMVEI DILASCDHPN IVKLLDAFYY
110 120 130 140 150
ENNLWILIEF CAGGAVDAVM LELERPLTES QIQVVCKQTL EALNYLHDNK
160 170 180 190 200
IIHRDLKAGN ILFTLDGDIK LADFGVSAKN TRTIQRRDSF IGTPYWMAPE
210 220 230 240 250
VVMCETSKDR PYDYKADVWS LGITLIEMAE IEPPHHELNP MRVLLKIAKS
260 270 280 290 300
EPPTLAQPSR WSSNFKDFLK KCLEKNVDAR WTTSQLLQHP FVTVDSNKPV
310 320 330 340 350
RELIAEAKAE VTEEVEDGKE EDDDDETESA LPIPANKRAS SDLSIASSEE
360 370 380 390 400
DKLSQNACIL ESVSERTEHN TSGDKFSNKV LSEKPTPEGP EKTVDVDGPA
410 420 430 440 450
NDVNLETVAE PNDQAVGFHE NGREKKRPQL ESQPDTEDQQ TVDVNLVGEG
460 470 480 490 500
NDSNIVILET NTDCLKPEED RNEENQEIIE NKLTQSEEIK DIHIQTMDLV
510 520 530 540 550
SQETGEKEAD FQAIDNEVGF TKEETQEKLG KDDKTHKVVI SDITSEVGTD
560 570 580 590 600
EPPGDTQKSA EQSQDAEGGA GEEAPEPAQT LTEKATEGPE AHGAEEEPRS
610 620 630 640 650
GERVEDKQLE QQSAVCEGEG QVTSTSESTR ATTEEPETDE VDQVSESNSI
660 670 680 690 700
EELERLGVTG AEEQALGSKG EAATELDLER EENAQELPVK AEPQAPAASQ
710 720 730 740 750
ASEPPPVLIP SINIHSENTE NKGEMGALPK PETILPPEPE NGKGNDTDSG
760 770 780 790 800
TGSTVENSSS DLNLSISSFL SKTKDSGSVS LQETRRQKKT LKKTRKFIVD
810 820 830 840 850
GVEVSVTTSK IVTDSDSKTE ELRFLRRQEL RELRLLQKEE QKAQQQLNGK
860 870 880 890 900
LQQQREQIFR RFEQEMLSKK RQYDQEIENL EKQQKQTIER LEQEHTNRLR
910 920 930 940 950
DEAKRIKGEQ EKELSKFQNM LRNRKKEEQE FVQKQQQELD GALKKIIQQQ
960 970 980 990 1000
KAELANIERE CLNNKQQLLR AREAAIWELE ERHLQEKHQL LKQQLKDQYF
1010 1020 1030 1040 1050
IQRHQLLKRH EKETEQMQRY NQRLIEELKN RQTQERARLP KIQRSEAKTR
1060 1070 1080 1090 1100
MAMFKKSLRI NSTATPDQDR EKIKQFAAQE EKRQKNERMA QHQKHESQMR
1110 1120 1130 1140 1150
DLQLQCEANV RELHQLQNEK CHLLVEHETQ KLKELDEEHS QELKEWREKL
1160 1170 1180 1190 1200
RPRKKTLEEE FARKLQEQEV FFKMTGESEC LNPSAQSRGC LQTSHPSSTR

APAWAG
Length:1,206
Mass (Da):137,888
Last modified:July 1, 1997 - v1
Checksum:i8F5C6A19A3501FB9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti165 – 1651L → I (PubMed:2062320).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003357 mRNA. Translation: BAA20077.1.
PIRiPT0204.
T34021.
RefSeqiNP_062222.2. NM_019349.2.
UniGeneiRn.228809.
Rn.33275.

Genome annotation databases

GeneIDi54308.
KEGGirno:54308.
UCSCiRGD:3780. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003357 mRNA. Translation: BAA20077.1.
PIRiPT0204.
T34021.
RefSeqiNP_062222.2. NM_019349.2.
UniGeneiRn.228809.
Rn.33275.

3D structure databases

ProteinModelPortaliO08815.
SMRiO08815. Positions 21-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO08815. 1 interaction.
STRINGi10116.ENSRNOP00000015496.

PTM databases

iPTMnetiO08815.
PhosphoSiteiO08815.

Proteomic databases

PaxDbiO08815.
PRIDEiO08815.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi54308.
KEGGirno:54308.
UCSCiRGD:3780. rat.

Organism-specific databases

CTDi9748.
RGDi3780. Slk.

Phylogenomic databases

eggNOGiKOG0579. Eukaryota.
ENOG410XPQN. LUCA.
HOGENOMiHOG000236268.
HOVERGENiHBG052712.
InParanoidiO08815.
KOiK08836.

Miscellaneous databases

PROiO08815.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR022165. PKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR001943. UVR_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF12474. PKK. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SK2, a putative rat homologue of yeast protein kinase NRK1."
    Fukami Y., Yamamoto H., Ichihara T., Mori K., Gomi T., Sato K.
    Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  2. "Novel putative protein kinase clones from a rat large granular lymphocyte tumor cell line."
    Yue C.C.
    Mol. Immunol. 28:399-408(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 156-214.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-340; SER-347; SER-348; SER-645; SER-649 AND SER-778, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSLK_RAT
AccessioniPrimary (citable) accession number: O08815
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: July 1, 1997
Last modified: June 8, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.