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O08811

- ERCC2_MOUSE

UniProt

O08811 - ERCC2_MOUSE

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Protein

TFIIH basal transcription factor complex helicase XPD subunit

Gene
Ercc2, Xpd
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

ATP-dependent 5'-3' DNA helicase, component of the core-TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers By similarity.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Magnesium By similarity.
Binds 1 4Fe-4S cluster By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi116 – 1161Iron-sulfur (4Fe-4S) By similarity
Metal bindingi134 – 1341Iron-sulfur (4Fe-4S) By similarity
Metal bindingi155 – 1551Iron-sulfur (4Fe-4S) By similarity
Metal bindingi190 – 1901Iron-sulfur (4Fe-4S) By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 498ATP By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. 5'-3' DNA helicase activity Source: UniProtKB
  3. ATP binding Source: UniProtKB-KW
  4. ATP-dependent DNA helicase activity Source: InterPro
  5. DNA binding Source: UniProtKB-KW
  6. metal ion binding Source: UniProtKB-KW
  7. RNA polymerase II carboxy-terminal domain kinase activity Source: Ensembl

GO - Biological processi

  1. aging Source: MGI
  2. apoptotic process Source: UniProtKB
  3. ATP catabolic process Source: GOC
  4. bone mineralization Source: MGI
  5. cell proliferation Source: MGI
  6. central nervous system myelin formation Source: MGI
  7. chromosome segregation Source: UniProtKB
  8. DNA duplex unwinding Source: GOC
  9. DNA repair Source: MGI
  10. embryonic cleavage Source: MGI
  11. erythrocyte maturation Source: MGI
  12. extracellular matrix organization Source: MGI
  13. hair cell differentiation Source: MGI
  14. hair cycle process Source: MGI
  15. hair follicle maturation Source: MGI
  16. hematopoietic stem cell differentiation Source: MGI
  17. in utero embryonic development Source: MGI
  18. multicellular organism growth Source: MGI
  19. nucleotide-excision repair Source: MGI
  20. nucleotide-excision repair, DNA incision Source: Ensembl
  21. positive regulation of DNA binding Source: MGI
  22. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  23. post-embryonic development Source: MGI
  24. regulation of mitotic cell cycle phase transition Source: Ensembl
  25. response to hypoxia Source: Ensembl
  26. response to oxidative stress Source: MGI
  27. response to UV Source: MGI
  28. skin development Source: MGI
  29. spinal cord development Source: MGI
  30. transcription-coupled nucleotide-excision repair Source: UniProtKB
  31. transcription from RNA polymerase II promoter Source: UniProtKB
  32. UV protection Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Chromosome partition, DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

4Fe-4S, ATP-binding, DNA-binding, Iron, Iron-sulfur, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_196637. Cytosolic iron-sulfur cluster assembly.
REACT_203462. Formation of the Early Elongation Complex.
REACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_224562. Dual incision reaction in TC-NER.
REACT_226490. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
TFIIH basal transcription factor complex helicase XPD subunit (EC:3.6.4.12)
Alternative name(s):
CXPD
DNA excision repair protein ERCC-2
DNA repair protein complementing XP-D cells
Xeroderma pigmentosum group D-complementing protein
Gene namesi
Name:Ercc2
Synonyms:Xpd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:95413. Ercc2.

Subcellular locationi

Nucleus. Cytoplasmcytoskeletonspindle By similarity

GO - Cellular componenti

  1. cyclin-dependent protein kinase activating kinase holoenzyme complex Source: Ensembl
  2. cytoplasm Source: UniProtKB
  3. holo TFIIH complex Source: UniProtKB
  4. MMXD complex Source: UniProtKB
  5. nucleus Source: MGI
  6. spindle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 760760TFIIH basal transcription factor complex helicase XPD subunitPRO_0000101981Add
BLAST

Post-translational modificationi

ISGylated By similarity.

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiO08811.
PRIDEiO08811.

PTM databases

PhosphoSiteiO08811.

Expressioni

Gene expression databases

BgeeiO08811.
GenevestigatoriO08811.

Interactioni

Subunit structurei

One of the six subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. The interaction with GTF2H2 results in the stimulation of the 5'-->3' helicase activity. Component of the MMXD complex, which includes CIAO1, ERCC2, FAM96B, MMS19 and SLC25A5. Interacts with FAM196B; the interaction is direct By similarity. Interacts with ATF7IP By similarity.

Protein-protein interaction databases

IntActiO08811. 1 interaction.
MINTiMINT-4114299.
STRINGi10090.ENSMUSP00000054380.

Structurei

3D structure databases

ProteinModelPortaliO08811.
SMRiO08811. Positions 26-258.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 283277Helicase ATP-bindingAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni438 – 637200Mediates interaction with MMS19 By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi234 – 2374DEAH box

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1199.
GeneTreeiENSGT00550000075092.
HOGENOMiHOG000205390.
HOVERGENiHBG051498.
InParanoidiQ8C487.
KOiK10844.
OMAiDEVWKYK.
OrthoDBiEOG70W3CM.
TreeFamiTF101232.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR013020. DNA_helicase_DNA-repair_Rad3.
IPR010643. DUF1227.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR027417. P-loop_NTPase.
IPR001945. XPGD_DNA_repair.
[Graphical view]
PfamiPF06733. DEAD_2. 1 hit.
PF06777. DUF1227. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
PRINTSiPR00852. XRODRMPGMNTD.
SMARTiSM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR00604. rad3. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08811-1 [UniParc]FASTAAdd to Basket

« Hide

MKLNVDGLLV YFPYDYIYPE QFSYMLELKR TLDAKGHGVL EMPSGTGKTV    50
SLLALIVAYQ RAYPLEVTKL IYCSRTVPEI EKVIEELRKL LSFYEQQEGE 100
KLPFLGLALS SRKNLCIHPE VTPLRFGKDV DGKCHSLTAS YVRAQYQQDA 150
SLPHCRFYEE FDIHGRQMPL PAGIYNLDDL KALGQRQGWC PYFLARYSIL 200
HANVVVYSYH YLLDPKIADL VSKELARKAV VVFDEAHNID NVCIDSMSVN 250
LTRRTLDRCQ SNLDTLQKTV LRIKETDEQR LRDEYRRLVE GLREASVARE 300
TDAHLANPVL PDEVLQEAVP GSIRTAEHFL GFLRRLLEYV KWRLRVQHVV 350
QESPPAFLSG LAQRVCIQRK PLRFCAERLR SLLHTLEIAD LADFSPLTLL 400
ANFATLVSTY AKGFTIIIEP FDDRTPTIAN PVLHFSCMDA SLAIKPVFER 450
FQSVIITSGT LSPLDIYPKI LDFHPVTMAT FTMTLARVCL CPMIIGRGND 500
QVAISSKFET REDIAVIRNY GNLLLEMSAV VPDGIVAFFT SYQYMESTVA 550
SWYEQGILEN IQRNKLLFIE TQDGAETSVA LEKYQEACEN GRGAILLSVA 600
RGKVSEGIDF VHHYGRAVIM FGVPYVYTQS RILKARLEYL RDQFQIREND 650
FLTFDAMRHA AQCVGRAIRG KTDYGLMVFA DKRFARADKR GKLPRWIQEH 700
LTDSNLNLTV DEGVQVAKYF LRQMAQPFHR EDQLGLSLLS LEQLQSEETL 750
QRIEQIAQQL 760
Length:760
Mass (Da):86,842
Last modified:July 27, 2011 - v2
Checksum:i02B2E116792D4E44
GO

Sequence cautioni

The sequence BAB23443.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti285 – 2851Y → C in BAB23443. 1 Publication
Sequence conflicti595 – 5951I → V in AAB58296. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U97572 mRNA. Translation: AAB58296.1.
AK004652 mRNA. Translation: BAB23443.1. Different initiation.
AK082761 mRNA. Translation: BAC38607.1.
CH466639 Genomic DNA. Translation: EDL23140.1.
CCDSiCCDS20900.1.
RefSeqiNP_031975.2. NM_007949.4.
UniGeneiMm.36524.

Genome annotation databases

EnsembliENSMUST00000062831; ENSMUSP00000054380; ENSMUSG00000030400.
GeneIDi13871.
KEGGimmu:13871.
UCSCiuc009flq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U97572 mRNA. Translation: AAB58296.1 .
AK004652 mRNA. Translation: BAB23443.1 . Different initiation.
AK082761 mRNA. Translation: BAC38607.1 .
CH466639 Genomic DNA. Translation: EDL23140.1 .
CCDSi CCDS20900.1.
RefSeqi NP_031975.2. NM_007949.4.
UniGenei Mm.36524.

3D structure databases

ProteinModelPortali O08811.
SMRi O08811. Positions 26-258.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O08811. 1 interaction.
MINTi MINT-4114299.
STRINGi 10090.ENSMUSP00000054380.

PTM databases

PhosphoSitei O08811.

Proteomic databases

PaxDbi O08811.
PRIDEi O08811.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000062831 ; ENSMUSP00000054380 ; ENSMUSG00000030400 .
GeneIDi 13871.
KEGGi mmu:13871.
UCSCi uc009flq.1. mouse.

Organism-specific databases

CTDi 2068.
MGIi MGI:95413. Ercc2.

Phylogenomic databases

eggNOGi COG1199.
GeneTreei ENSGT00550000075092.
HOGENOMi HOG000205390.
HOVERGENi HBG051498.
InParanoidi Q8C487.
KOi K10844.
OMAi DEVWKYK.
OrthoDBi EOG70W3CM.
TreeFami TF101232.

Enzyme and pathway databases

Reactomei REACT_196637. Cytosolic iron-sulfur cluster assembly.
REACT_203462. Formation of the Early Elongation Complex.
REACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_224562. Dual incision reaction in TC-NER.
REACT_226490. RNA Polymerase II Transcription Elongation.

Miscellaneous databases

NextBioi 284788.
PROi O08811.
SOURCEi Search...

Gene expression databases

Bgeei O08811.
Genevestigatori O08811.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR013020. DNA_helicase_DNA-repair_Rad3.
IPR010643. DUF1227.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR027417. P-loop_NTPase.
IPR001945. XPGD_DNA_repair.
[Graphical view ]
Pfami PF06733. DEAD_2. 1 hit.
PF06777. DUF1227. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view ]
PRINTSi PR00852. XRODRMPGMNTD.
SMARTi SM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 4 hits.
TIGRFAMsi TIGR00604. rad3. 1 hit.
PROSITEi PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Disruption of the mouse xeroderma pigmentosum group D DNA repair/basal transcription gene results in preimplantation lethality."
    de Boer J., Donker I., de Wit J., Hoeijmakers J.H.J., Weeda G.
    Cancer Res. 58:89-94(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Lung.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiERCC2_MOUSE
AccessioniPrimary (citable) accession number: O08811
Secondary accession number(s): Q8C487, Q9DC01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi