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Protein

116 kDa U5 small nuclear ribonucleoprotein component

Gene

Eftud2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex required for pre-mRNA splicing. Binds GTP (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi135 – 1428GTPSequence Analysis
Nucleotide bindingi203 – 2075GTPSequence Analysis
Nucleotide bindingi257 – 2604GTPSequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_271571. mRNA Splicing - Minor Pathway.
REACT_307866. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
116 kDa U5 small nuclear ribonucleoprotein component
Alternative name(s):
Elongation factor Tu GTP-binding domain-containing protein 2
U5 snRNP-specific protein, 116 kDa
Short name:
U5-116 kDa
Gene namesi
Name:Eftud2
Synonyms:Snrp116
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1336880. Eftud2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 971971116 kDa U5 small nuclear ribonucleoprotein componentPRO_0000091564Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei19 – 191PhosphoserineBy similarity
Modified residuei85 – 851PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO08810.
PaxDbiO08810.
PRIDEiO08810.

PTM databases

PhosphoSiteiO08810.

Expressioni

Gene expression databases

BgeeiO08810.
ExpressionAtlasiO08810. baseline and differential.
GenevestigatoriO08810.

Interactioni

Subunit structurei

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39. Identified in the spliceosome C complex. Interacts with ERBB4 and PRPF8. Interacts with PIH1D1.By similarity

Protein-protein interaction databases

BioGridi203372. 1 interaction.
IntActiO08810. 5 interactions.
MINTiMINT-1868092.

Structurei

3D structure databases

ProteinModelPortaliO08810.
SMRiO08810. Positions 113-946.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini126 – 408283tr-type GPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0480.
HOVERGENiHBG001838.
InParanoidiO08810.
KOiK12852.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08810-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEDEDED EDDVGEHEDD
60 70 80 90 100
HPGMEVVLHE DKKYYPTAEE VYGPEVETIV QEEDTQPLTE PIIKPVKTKK
110 120 130 140 150
FTLMEQTLPV TVYEMDFLAD LMDNSELIRN VTLCGHLHHG KTCFVDCLIE
160 170 180 190 200
QTHPEIRKRY DQDLCYTDIL FTEQERGVGI KSTPVTVVLP DTKGKSYLFN
210 220 230 240 250
IMDTPGHVNF SDEVTAGLRI SDGVVLFIDA AEGVMLNTER LIKHAVQERL
260 270 280 290 300
AVTVCINKID RLILELKLPP TDAYYKLRHI VDEVNGLISM YSTDENLILS
310 320 330 340 350
PLLGNVCFSS SQYSICFTLG SFAKIYADTF GDINYQEFAK RLWGDIYFNP
360 370 380 390 400
KTRKFTKKAP SSSSQRSFVE FILEPLYKIL AQVVGDVDTS LPRTLDELGI
410 420 430 440 450
HLTKEELKLN IRPLLRLVCK KFFGEFTGFV DMCVQHIPSP KVGAKPKIEH
460 470 480 490 500
TYTGGVDSDL GEAMSDCDPD GPLMCHTTKM YSTDDGVQFH AFGRVLSGTI
510 520 530 540 550
HAGQPVKVLG ENYTLEDEED SQICTVGRLW ISVARYHIEV NRVPAGNWVL
560 570 580 590 600
IEGVDQPIVK TATITEPRGN EEAQIFRPLK FNTTSVIKIA VEPVNPSELP
610 620 630 640 650
KMLDGLRKVN KSYPSLTTKV EESGEHVILG TGELYLDCVM HDLRKMYSEI
660 670 680 690 700
DIKVADPVVT FCETVVETSS LKCFAETPNK KNKITMIAEP LEKGLAEDIE
710 720 730 740 750
NEVVQITWNR KKLGEFFQTK YDWDLLAARS IWAFGPDATG PNILVDDTLP
760 770 780 790 800
SEVDKALLGS VKDSIVQGFQ WGTREGPLCD ELIRNVKFKI LDAVVAQEPL
810 820 830 840 850
HRGGGQIIPT ARRVVYSAFL MATPRLMEPY YFVEVQAPAD CVSAVYTVLA
860 870 880 890 900
RRRGHVTQDA PIPGSPLYTI KAFIPAIDSF GFETDLRTHT QGQAFSLSVF
910 920 930 940 950
HHWQIVPGDP LDKSIVIRPL EPQPAPHLAR EFMIKTRRRK GLSEDVSISK
960 970
FFDDPMLLEL AKQDVVLNYP M
Length:971
Mass (Da):109,361
Last modified:July 1, 1997 - v1
Checksum:i0ECF1661DEA3A7FC
GO

Sequence cautioni

The sequence AAH12636.1 differs from that shown.Contaminating sequence. Vector contamination at the N-terminus.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97079 mRNA. Translation: AAC53299.1.
BC012636 mRNA. Translation: AAH12636.1. Different initiation.
BC054778 mRNA. Translation: AAH54778.1.
CCDSiCCDS48947.1.
RefSeqiNP_001103465.1. NM_001109995.1.
NP_035561.1. NM_011431.3.
UniGeneiMm.873.

Genome annotation databases

EnsembliENSMUST00000107060; ENSMUSP00000102675; ENSMUSG00000020929.
GeneIDi20624.
KEGGimmu:20624.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97079 mRNA. Translation: AAC53299.1.
BC012636 mRNA. Translation: AAH12636.1. Different initiation.
BC054778 mRNA. Translation: AAH54778.1.
CCDSiCCDS48947.1.
RefSeqiNP_001103465.1. NM_001109995.1.
NP_035561.1. NM_011431.3.
UniGeneiMm.873.

3D structure databases

ProteinModelPortaliO08810.
SMRiO08810. Positions 113-946.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203372. 1 interaction.
IntActiO08810. 5 interactions.
MINTiMINT-1868092.

PTM databases

PhosphoSiteiO08810.

Proteomic databases

MaxQBiO08810.
PaxDbiO08810.
PRIDEiO08810.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000107060; ENSMUSP00000102675; ENSMUSG00000020929.
GeneIDi20624.
KEGGimmu:20624.

Organism-specific databases

CTDi9343.
MGIiMGI:1336880. Eftud2.

Phylogenomic databases

eggNOGiCOG0480.
HOVERGENiHBG001838.
InParanoidiO08810.
KOiK12852.

Enzyme and pathway databases

ReactomeiREACT_271571. mRNA Splicing - Minor Pathway.
REACT_307866. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiEftud2. mouse.
NextBioi299023.
PROiO08810.
SOURCEiSearch...

Gene expression databases

BgeeiO08810.
ExpressionAtlasiO08810. baseline and differential.
GenevestigatoriO08810.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2."
    Fabrizio P., Laggerbauer B., Lauber J., Lane W.S., Luehrmann R.
    EMBO J. 16:4092-4106(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain and Mammary gland.

Entry informationi

Entry nameiU5S1_MOUSE
AccessioniPrimary (citable) accession number: O08810
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 1, 1997
Last modified: May 27, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.