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Protein

116 kDa U5 small nuclear ribonucleoprotein component

Gene

Eftud2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex required for pre-mRNA splicing. Binds GTP (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi135 – 142GTPSequence analysis8
Nucleotide bindingi203 – 207GTPSequence analysis5
Nucleotide bindingi257 – 260GTPSequence analysis4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
116 kDa U5 small nuclear ribonucleoprotein component
Alternative name(s):
Elongation factor Tu GTP-binding domain-containing protein 2
U5 snRNP-specific protein, 116 kDa
Short name:
U5-116 kDa
Gene namesi
Name:Eftud2
Synonyms:Snrp116
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1336880 Eftud2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000915641 – 971116 kDa U5 small nuclear ribonucleoprotein componentAdd BLAST971

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei19PhosphoserineBy similarity1
Cross-linki63Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki63Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei85PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO08810
PaxDbiO08810
PeptideAtlasiO08810
PRIDEiO08810

PTM databases

iPTMnetiO08810
PhosphoSitePlusiO08810
SwissPalmiO08810

Expressioni

Gene expression databases

BgeeiENSMUSG00000020929
ExpressionAtlasiO08810 baseline and differential
GenevisibleiO08810 MM

Interactioni

Subunit structurei

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Identified in the spliceosome C complex. Interacts with ERBB4 and PRPF8. Interacts with PIH1D1.By similarity

Protein-protein interaction databases

BioGridi203372, 5 interactors
IntActiO08810, 7 interactors
MINTiO08810
STRINGi10090.ENSMUSP00000021306

Structurei

3D structure databases

ProteinModelPortaliO08810
SMRiO08810
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini126 – 408tr-type GPROSITE-ProRule annotationAdd BLAST283

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0469 Eukaryota
COG0480 LUCA
GeneTreeiENSGT00870000136483
HOVERGENiHBG001838
InParanoidiO08810
KOiK12852

Family and domain databases

CDDicd04098 eEF2_C_snRNP, 1 hit
Gene3Di3.30.230.10, 2 hits
InterProiView protein in InterPro
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031950 EFTUD2_N
IPR027417 P-loop_NTPase
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR005517 Transl_elong_EFG/EF2_IV
IPR035655 U5-116kDa_C
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF03764 EFG_IV, 1 hit
PF16004 EFTUD2, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SM00889 EFG_IV, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54211 SSF54211, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

O08810-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEDEDED EDDVGEHEDD
60 70 80 90 100
HPGMEVVLHE DKKYYPTAEE VYGPEVETIV QEEDTQPLTE PIIKPVKTKK
110 120 130 140 150
FTLMEQTLPV TVYEMDFLAD LMDNSELIRN VTLCGHLHHG KTCFVDCLIE
160 170 180 190 200
QTHPEIRKRY DQDLCYTDIL FTEQERGVGI KSTPVTVVLP DTKGKSYLFN
210 220 230 240 250
IMDTPGHVNF SDEVTAGLRI SDGVVLFIDA AEGVMLNTER LIKHAVQERL
260 270 280 290 300
AVTVCINKID RLILELKLPP TDAYYKLRHI VDEVNGLISM YSTDENLILS
310 320 330 340 350
PLLGNVCFSS SQYSICFTLG SFAKIYADTF GDINYQEFAK RLWGDIYFNP
360 370 380 390 400
KTRKFTKKAP SSSSQRSFVE FILEPLYKIL AQVVGDVDTS LPRTLDELGI
410 420 430 440 450
HLTKEELKLN IRPLLRLVCK KFFGEFTGFV DMCVQHIPSP KVGAKPKIEH
460 470 480 490 500
TYTGGVDSDL GEAMSDCDPD GPLMCHTTKM YSTDDGVQFH AFGRVLSGTI
510 520 530 540 550
HAGQPVKVLG ENYTLEDEED SQICTVGRLW ISVARYHIEV NRVPAGNWVL
560 570 580 590 600
IEGVDQPIVK TATITEPRGN EEAQIFRPLK FNTTSVIKIA VEPVNPSELP
610 620 630 640 650
KMLDGLRKVN KSYPSLTTKV EESGEHVILG TGELYLDCVM HDLRKMYSEI
660 670 680 690 700
DIKVADPVVT FCETVVETSS LKCFAETPNK KNKITMIAEP LEKGLAEDIE
710 720 730 740 750
NEVVQITWNR KKLGEFFQTK YDWDLLAARS IWAFGPDATG PNILVDDTLP
760 770 780 790 800
SEVDKALLGS VKDSIVQGFQ WGTREGPLCD ELIRNVKFKI LDAVVAQEPL
810 820 830 840 850
HRGGGQIIPT ARRVVYSAFL MATPRLMEPY YFVEVQAPAD CVSAVYTVLA
860 870 880 890 900
RRRGHVTQDA PIPGSPLYTI KAFIPAIDSF GFETDLRTHT QGQAFSLSVF
910 920 930 940 950
HHWQIVPGDP LDKSIVIRPL EPQPAPHLAR EFMIKTRRRK GLSEDVSISK
960 970
FFDDPMLLEL AKQDVVLNYP M
Length:971
Mass (Da):109,361
Last modified:July 1, 1997 - v1
Checksum:i0ECF1661DEA3A7FC
GO

Sequence cautioni

The sequence AAH12636 differs from that shown. Contaminating sequence. Vector contamination at the N-terminus.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97079 mRNA Translation: AAC53299.1
BC012636 mRNA Translation: AAH12636.1 Different initiation.
BC054778 mRNA Translation: AAH54778.1
CCDSiCCDS48947.1
RefSeqiNP_001103465.1, NM_001109995.1
NP_035561.1, NM_011431.3
UniGeneiMm.873

Genome annotation databases

EnsembliENSMUST00000107060; ENSMUSP00000102675; ENSMUSG00000020929
GeneIDi20624
KEGGimmu:20624
UCSCiuc033gal.1 mouse

Similar proteinsi

Entry informationi

Entry nameiU5S1_MOUSE
AccessioniPrimary (citable) accession number: O08810
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 1, 1997
Last modified: May 23, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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