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O08810

- U5S1_MOUSE

UniProt

O08810 - U5S1_MOUSE

Protein

116 kDa U5 small nuclear ribonucleoprotein component

Gene

Eftud2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex required for pre-mRNA splicing. Binds GTP By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi135 – 1428GTPSequence Analysis
    Nucleotide bindingi203 – 2075GTPSequence Analysis
    Nucleotide bindingi257 – 2604GTPSequence Analysis

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. RNA splicing Source: UniProtKB-KW

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    116 kDa U5 small nuclear ribonucleoprotein component
    Alternative name(s):
    Elongation factor Tu GTP-binding domain-containing protein 2
    U5 snRNP-specific protein, 116 kDa
    Short name:
    U5-116 kDa
    Gene namesi
    Name:Eftud2
    Synonyms:Snrp116
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1336880. Eftud2.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: MGI
    2. spliceosomal complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 971971116 kDa U5 small nuclear ribonucleoprotein componentPRO_0000091564Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei19 – 191PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO08810.
    PaxDbiO08810.
    PRIDEiO08810.

    PTM databases

    PhosphoSiteiO08810.

    Expressioni

    Gene expression databases

    ArrayExpressiO08810.
    BgeeiO08810.
    GenevestigatoriO08810.

    Interactioni

    Subunit structurei

    Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39. Identified in the spliceosome C complex. Interacts with ERBB4 and PRPF8 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi203372. 1 interaction.
    IntActiO08810. 5 interactions.
    MINTiMINT-1868092.

    Structurei

    3D structure databases

    ProteinModelPortaliO08810.
    SMRiO08810. Positions 113-953.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini126 – 408283tr-type GPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation
    Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0480.
    HOVERGENiHBG001838.
    KOiK12852.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR027417. P-loop_NTPase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR005517. Transl_elong_EFG/EF2_IV.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00679. EFG_C. 1 hit.
    PF14492. EFG_II. 1 hit.
    PF03764. EFG_IV. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SMARTiSM00838. EFG_C. 1 hit.
    SM00889. EFG_IV. 1 hit.
    [Graphical view]
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54211. SSF54211. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O08810-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEDEDED EDDVGEHEDD    50
    HPGMEVVLHE DKKYYPTAEE VYGPEVETIV QEEDTQPLTE PIIKPVKTKK 100
    FTLMEQTLPV TVYEMDFLAD LMDNSELIRN VTLCGHLHHG KTCFVDCLIE 150
    QTHPEIRKRY DQDLCYTDIL FTEQERGVGI KSTPVTVVLP DTKGKSYLFN 200
    IMDTPGHVNF SDEVTAGLRI SDGVVLFIDA AEGVMLNTER LIKHAVQERL 250
    AVTVCINKID RLILELKLPP TDAYYKLRHI VDEVNGLISM YSTDENLILS 300
    PLLGNVCFSS SQYSICFTLG SFAKIYADTF GDINYQEFAK RLWGDIYFNP 350
    KTRKFTKKAP SSSSQRSFVE FILEPLYKIL AQVVGDVDTS LPRTLDELGI 400
    HLTKEELKLN IRPLLRLVCK KFFGEFTGFV DMCVQHIPSP KVGAKPKIEH 450
    TYTGGVDSDL GEAMSDCDPD GPLMCHTTKM YSTDDGVQFH AFGRVLSGTI 500
    HAGQPVKVLG ENYTLEDEED SQICTVGRLW ISVARYHIEV NRVPAGNWVL 550
    IEGVDQPIVK TATITEPRGN EEAQIFRPLK FNTTSVIKIA VEPVNPSELP 600
    KMLDGLRKVN KSYPSLTTKV EESGEHVILG TGELYLDCVM HDLRKMYSEI 650
    DIKVADPVVT FCETVVETSS LKCFAETPNK KNKITMIAEP LEKGLAEDIE 700
    NEVVQITWNR KKLGEFFQTK YDWDLLAARS IWAFGPDATG PNILVDDTLP 750
    SEVDKALLGS VKDSIVQGFQ WGTREGPLCD ELIRNVKFKI LDAVVAQEPL 800
    HRGGGQIIPT ARRVVYSAFL MATPRLMEPY YFVEVQAPAD CVSAVYTVLA 850
    RRRGHVTQDA PIPGSPLYTI KAFIPAIDSF GFETDLRTHT QGQAFSLSVF 900
    HHWQIVPGDP LDKSIVIRPL EPQPAPHLAR EFMIKTRRRK GLSEDVSISK 950
    FFDDPMLLEL AKQDVVLNYP M 971
    Length:971
    Mass (Da):109,361
    Last modified:July 1, 1997 - v1
    Checksum:i0ECF1661DEA3A7FC
    GO

    Sequence cautioni

    The sequence AAH12636.1 differs from that shown. Reason: Contaminating sequence. Vector contamination at the N-terminus.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U97079 mRNA. Translation: AAC53299.1.
    BC012636 mRNA. Translation: AAH12636.1. Different initiation.
    BC054778 mRNA. Translation: AAH54778.1.
    CCDSiCCDS48947.1.
    RefSeqiNP_001103465.1. NM_001109995.1.
    NP_035561.1. NM_011431.3.
    UniGeneiMm.873.

    Genome annotation databases

    EnsembliENSMUST00000107060; ENSMUSP00000102675; ENSMUSG00000020929.
    GeneIDi20624.
    KEGGimmu:20624.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U97079 mRNA. Translation: AAC53299.1 .
    BC012636 mRNA. Translation: AAH12636.1 . Different initiation.
    BC054778 mRNA. Translation: AAH54778.1 .
    CCDSi CCDS48947.1.
    RefSeqi NP_001103465.1. NM_001109995.1.
    NP_035561.1. NM_011431.3.
    UniGenei Mm.873.

    3D structure databases

    ProteinModelPortali O08810.
    SMRi O08810. Positions 113-953.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203372. 1 interaction.
    IntActi O08810. 5 interactions.
    MINTi MINT-1868092.

    PTM databases

    PhosphoSitei O08810.

    Proteomic databases

    MaxQBi O08810.
    PaxDbi O08810.
    PRIDEi O08810.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000107060 ; ENSMUSP00000102675 ; ENSMUSG00000020929 .
    GeneIDi 20624.
    KEGGi mmu:20624.

    Organism-specific databases

    CTDi 9343.
    MGIi MGI:1336880. Eftud2.

    Phylogenomic databases

    eggNOGi COG0480.
    HOVERGENi HBG001838.
    KOi K12852.

    Miscellaneous databases

    ChiTaRSi EFTUD2. mouse.
    NextBioi 299023.
    PROi O08810.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O08810.
    Bgeei O08810.
    Genevestigatori O08810.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR027417. P-loop_NTPase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR005517. Transl_elong_EFG/EF2_IV.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00679. EFG_C. 1 hit.
    PF14492. EFG_II. 1 hit.
    PF03764. EFG_IV. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SMARTi SM00838. EFG_C. 1 hit.
    SM00889. EFG_IV. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54211. SSF54211. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2."
      Fabrizio P., Laggerbauer B., Lauber J., Lane W.S., Luehrmann R.
      EMBO J. 16:4092-4106(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Mammary gland.

    Entry informationi

    Entry nameiU5S1_MOUSE
    AccessioniPrimary (citable) accession number: O08810
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3