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O08810 (U5S1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
116 kDa U5 small nuclear ribonucleoprotein component
Alternative name(s):
Elongation factor Tu GTP-binding domain-containing protein 2
U5 snRNP-specific protein, 116 kDa
Short name=U5-116 kDa
Gene names
Name:Eftud2
Synonyms:Snrp116
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length971 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex required for pre-mRNA splicing. Binds GTP By similarity.

Subunit structure

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39. Identified in the spliceosome C complex. Interacts with ERBB4 and PRPF8 By similarity.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Sequence caution

The sequence AAH12636.1 differs from that shown. Reason: Contaminating sequence. Vector contamination at the N-terminus.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentNucleus
Spliceosome
   LigandGTP-binding
Nucleotide-binding
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA splicing

Inferred from electronic annotation. Source: UniProtKB-KW

mRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay PubMed 11739632. Source: MGI

spliceosomal complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 971971116 kDa U5 small nuclear ribonucleoprotein component
PRO_0000091564

Regions

Nucleotide binding135 – 1428GTP Potential
Nucleotide binding203 – 2075GTP Potential
Nucleotide binding257 – 2604GTP Potential

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue191Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
O08810 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 0ECF1661DEA3A7FC

FASTA971109,361
        10         20         30         40         50         60 
MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEDEDED EDDVGEHEDD HPGMEVVLHE 

        70         80         90        100        110        120 
DKKYYPTAEE VYGPEVETIV QEEDTQPLTE PIIKPVKTKK FTLMEQTLPV TVYEMDFLAD 

       130        140        150        160        170        180 
LMDNSELIRN VTLCGHLHHG KTCFVDCLIE QTHPEIRKRY DQDLCYTDIL FTEQERGVGI 

       190        200        210        220        230        240 
KSTPVTVVLP DTKGKSYLFN IMDTPGHVNF SDEVTAGLRI SDGVVLFIDA AEGVMLNTER 

       250        260        270        280        290        300 
LIKHAVQERL AVTVCINKID RLILELKLPP TDAYYKLRHI VDEVNGLISM YSTDENLILS 

       310        320        330        340        350        360 
PLLGNVCFSS SQYSICFTLG SFAKIYADTF GDINYQEFAK RLWGDIYFNP KTRKFTKKAP 

       370        380        390        400        410        420 
SSSSQRSFVE FILEPLYKIL AQVVGDVDTS LPRTLDELGI HLTKEELKLN IRPLLRLVCK 

       430        440        450        460        470        480 
KFFGEFTGFV DMCVQHIPSP KVGAKPKIEH TYTGGVDSDL GEAMSDCDPD GPLMCHTTKM 

       490        500        510        520        530        540 
YSTDDGVQFH AFGRVLSGTI HAGQPVKVLG ENYTLEDEED SQICTVGRLW ISVARYHIEV 

       550        560        570        580        590        600 
NRVPAGNWVL IEGVDQPIVK TATITEPRGN EEAQIFRPLK FNTTSVIKIA VEPVNPSELP 

       610        620        630        640        650        660 
KMLDGLRKVN KSYPSLTTKV EESGEHVILG TGELYLDCVM HDLRKMYSEI DIKVADPVVT 

       670        680        690        700        710        720 
FCETVVETSS LKCFAETPNK KNKITMIAEP LEKGLAEDIE NEVVQITWNR KKLGEFFQTK 

       730        740        750        760        770        780 
YDWDLLAARS IWAFGPDATG PNILVDDTLP SEVDKALLGS VKDSIVQGFQ WGTREGPLCD 

       790        800        810        820        830        840 
ELIRNVKFKI LDAVVAQEPL HRGGGQIIPT ARRVVYSAFL MATPRLMEPY YFVEVQAPAD 

       850        860        870        880        890        900 
CVSAVYTVLA RRRGHVTQDA PIPGSPLYTI KAFIPAIDSF GFETDLRTHT QGQAFSLSVF 

       910        920        930        940        950        960 
HHWQIVPGDP LDKSIVIRPL EPQPAPHLAR EFMIKTRRRK GLSEDVSISK FFDDPMLLEL 

       970 
AKQDVVLNYP M 

« Hide

References

« Hide 'large scale' references
[1]"An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2."
Fabrizio P., Laggerbauer B., Lauber J., Lane W.S., Luehrmann R.
EMBO J. 16:4092-4106(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U97079 mRNA. Translation: AAC53299.1.
BC012636 mRNA. Translation: AAH12636.1. Different initiation.
BC054778 mRNA. Translation: AAH54778.1.
CCDSCCDS48947.1.
RefSeqNP_001103465.1. NM_001109995.1.
NP_035561.1. NM_011431.3.
UniGeneMm.873.

3D structure databases

ProteinModelPortalO08810.
SMRO08810. Positions 113-953.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203372. 1 interaction.
IntActO08810. 5 interactions.
MINTMINT-1868092.

PTM databases

PhosphoSiteO08810.

Proteomic databases

MaxQBO08810.
PaxDbO08810.
PRIDEO08810.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000107060; ENSMUSP00000102675; ENSMUSG00000020929.
GeneID20624.
KEGGmmu:20624.

Organism-specific databases

CTD9343.
MGIMGI:1336880. Eftud2.

Phylogenomic databases

eggNOGCOG0480.
HOVERGENHBG001838.
KOK12852.

Gene expression databases

ArrayExpressO08810.
BgeeO08810.
GenevestigatorO08810.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SMARTSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Other

ChiTaRSEFTUD2. mouse.
NextBio299023.
PROO08810.
SOURCESearch...

Entry information

Entry nameU5S1_MOUSE
AccessionPrimary (citable) accession number: O08810
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot