Reviewed,
UniProtKB/Swiss-Prot O08807 (PRDX4_MOUSE)
Last modified
June 16, 2009.
Version 89.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Peroxiredoxin-4 EC=1.11.1.15 Alternative name(s): Prx-IV Thioredoxin peroxidase AO372 Thioredoxin-dependent peroxide reductase A0372 Antioxidant enzyme AOE372 | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 274 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. |
| Catalytic activity | 2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH. |
| Subunit structure | Homodimer or heterodimer with PRDX1; disulfide-linked, upon oxidation. |
| Subcellular location | Cytoplasm By similarity. |
| Miscellaneous | The active site is the redox-active Cys-127 oxidized to Cys-SOH. Cys-SOH rapidly reacts with Cys-248-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin By similarity. Irreversibly inactivated by overoxidation of Cys-127 (to Cys-SO3H) upon oxidative stress By similarity. |
| Sequence similarities | Belongs to the ahpC/TSA family. Contains 1 thioredoxin domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Domain | Redox-active center |
| Molecular function | Antioxidant Oxidoreductase Peroxidase |
| PTM | Disulfide bond |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | mitochondrion Inferred from direct assay. Source: MGI |
| Molecular function | peroxiredoxin activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 274 | 274 | Peroxiredoxin-4 | PRO_0000135099 | |||||
Regions | |||||||||
| Domain | 82 – 240 | 159 | Thioredoxin | ||||||
Sites | |||||||||
| Active site | 127 | 1 | Cysteine sulfenic acid (-SOH) intermediate By similarity | ||||||
Amino acid modifications | |||||||||
| Disulfide bond | 127 | Interchain (with C-248); in linked form By similarity | |||||||
| Disulfide bond | 248 | Interchain (with C-127); in linked form By similarity | |||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Characterization of human and mouse peroxiredoxin IV: evidence for inhibition by Prx-IV of epidermal growth factor- and p53-induced reactive oxygen species." Wong C.M., Chun A.C., Kok K.H., Zhou Y., Fung P.C., Kung H.F., Jeang K.-T., Jin D.-Y. Antioxid. Redox Signal. 2:507-518(2000) [PubMed: 11229364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. |
| [2] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and DBA/2. Tissue: Bone marrow and Liver. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NMRI. Tissue: Mammary gland and Mammary tumor. |
| [4] | Lubec G., Kang S.U., Yang J.W., Zigmond M. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 190-203 AND 216-226. Strain: C57BL/6. Tissue: Brain. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U96746 mRNA. Translation: AAB57846.1. AK005031 mRNA. Translation: BAB23758.1. AK146402 mRNA. Translation: BAE27143.1. AK152255 mRNA. Translation: BAE31074.1. BC003349 mRNA. Translation: AAH03349.1. BC019578 mRNA. Translation: AAH19578.1. | |
| IPI | IPI00116254. |
| RefSeq | NP_058044.1. |
| UniGene | Mm.247542 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1QQ2 based on UniProtKB Q63716. |
| ModBase | Search... |
Protein family/group databases | |
| PeroxiBase | 4532. Mm2CysPrx04. |
2-D gel databases | |
| PMMA-2DPAGE | O08807. |
| REPRODUCTION-2DPAGE | O08807. |
Proteomic databases | |
| PRIDE | O08807. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000025289. Mus musculus. [Contig view] |
| GeneID | 53381. |
| KEGG | mmu:53381. |
| NMPDR | fig|10090.3.peg.22366. |
Organism-specific databases | |
| MGI | MGI:1859815. Prdx4. |
Phylogenomic databases | |
| HOGENOM | O08807. |
| HOVERGEN | O08807. |
| OMA | O08807. YLEEAGH. |
Enzyme and pathway databases | |
| BRENDA | 1.11.1.15. 244. |
Gene expression databases | |
| ArrayExpress | O08807. |
| Bgee | O08807. |
| CleanEx | MM_PRDX4. |
| GermOnline | ENSMUSG00000025289. Mus musculus. |
Family and domain databases | |
| InterPro | IPR000866. Alkyl_hydroperoxide_Rdtase. IPR019479. Peroxiredoxin_C. IPR017936. Thioredoxin-like. IPR012335. Thioredoxin_fold. [Graphical view] |
| Gene3D | G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit. |
| Pfam | PF10417. 1-cysPrx_C. 1 hit. PF00578. AhpC-TSA. 1 hit. [Graphical view] |
| PROSITE | PS51352. THIOREDOXIN_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 310209. |
| SOURCE | Search... |
Entry information
| Entry name | PRDX4_MOUSE | ||||||||
| Accession | Primary (citable) accession number: O08807 Secondary accession number(s): Q3U8E4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


