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O08796

- EF2K_MOUSE

UniProt

O08796 - EF2K_MOUSE

Protein

Eukaryotic elongation factor 2 kinase

Gene

Eef2k

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.1 Publication

    Catalytic activityi

    ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.1 Publication

    Enzyme regulationi

    Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi295 – 3017ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. calmodulin binding Source: UniProtKB
    4. elongation factor-2 kinase activity Source: UniProtKB
    5. translation factor activity, nucleic acid binding Source: UniProtKB

    GO - Biological processi

    1. protein autophosphorylation Source: Ensembl
    2. regulation of protein autophosphorylation Source: UniProtKB
    3. translational elongation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calcium, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.20. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic elongation factor 2 kinase (EC:2.7.11.20)
    Short name:
    eEF-2 kinase
    Short name:
    eEF-2K
    Alternative name(s):
    Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
    Gene namesi
    Name:Eef2k
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1195261. Eef2k.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 724723Eukaryotic elongation factor 2 kinasePRO_0000086937Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei61 – 611Phosphoserine; by autocatalysis
    Modified residuei70 – 701Phosphoserine1 Publication
    Modified residuei77 – 771Phosphoserine; by autocatalysis and TRPM7By similarity
    Modified residuei347 – 3471Phosphothreonine; by autocatalysisBy similarity
    Modified residuei352 – 3521Phosphothreonine; by autocatalysisBy similarity
    Modified residuei358 – 3581Phosphoserine; by MAPK13 and CDK1By similarity
    Modified residuei365 – 3651Phosphoserine; by autocatalysis, RPS6KA1 and RPS6KB1By similarity
    Modified residuei397 – 3971Phosphoserine; by AMPKBy similarity
    Modified residuei444 – 4441Phosphoserine; by autocatalysisBy similarity
    Modified residuei469 – 4691PhosphoserineBy similarity
    Modified residuei473 – 4731Phosphoserine; by autocatalysis
    Modified residuei476 – 4761PhosphoserineBy similarity
    Modified residuei499 – 4991Phosphoserine; by PKABy similarity

    Post-translational modificationi

    Autophosphorylated at multiple residues, Thr-347 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-397 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-77 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-358 or RPS6KA1 and RPS6KB1 at Ser-365 instead decrease EEF2K activity and promote protein synthesis By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO08796.
    PaxDbiO08796.
    PRIDEiO08796.

    PTM databases

    PhosphoSiteiO08796.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed. Particularly abundant in skeletal muscle and heart.

    Gene expression databases

    ArrayExpressiO08796.
    BgeeiO08796.
    CleanExiMM_EEF2K.
    GenevestigatoriO08796.

    Interactioni

    Subunit structurei

    Monomer or homodimer.Curated

    Structurei

    3D structure databases

    ProteinModelPortaliO08796.
    SMRiO08796. Positions 116-322, 526-646.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini115 – 325211Alpha-type protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni593 – 60917Calmodulin-bindingSequence AnalysisAdd
    BLAST
    Regioni609 – 62618Pseudosubstrate/autoinhibitory domainSequence AnalysisAdd
    BLAST

    Domaini

    The catalytic domain is located to N-terminal region. The neighbor region contains the calmodulin-binding domain By similarity.By similarity

    Sequence similaritiesi

    Contains 1 alpha-type protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG145133.
    GeneTreeiENSGT00740000115539.
    HOGENOMiHOG000022023.
    HOVERGENiHBG002318.
    InParanoidiO08796.
    KOiK08292.
    OMAiQIHTETG.
    OrthoDBiEOG7673B0.
    PhylomeDBiO08796.
    TreeFamiTF316085.

    Family and domain databases

    Gene3Di1.25.40.10. 1 hit.
    InterProiIPR017400. Elongation_factor_2_kinase.
    IPR011009. Kinase-like_dom.
    IPR004166. MHCK_EF2_kinase.
    IPR006597. Sel1-like.
    IPR011990. TPR-like_helical.
    [Graphical view]
    PfamiPF02816. Alpha_kinase. 1 hit.
    PF08238. Sel1. 4 hits.
    [Graphical view]
    PIRSFiPIRSF038139. Elongation_factor_2_kinase. 1 hit.
    SMARTiSM00811. Alpha_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS51158. ALPHA_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O08796-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADEDLIFCL EGVDGGRCSR AGHNADSDTD SDDDEGYFIC PITDDHMSNQ    50
    NVSSKVQSYY SNLTKTECGS TGSPASSFHF KEAWKHAIEK AKHMPDPWAE 100
    FHLEDIATEH ATRHRYNAVT GEWLKDEVLI KMASQPFGRG AMRECFRTKK 150
    LSNFLHAQQW KGASNYVAKR YIEPVDRSVY FEDVQLQMEA KLWGEDYNRH 200
    KPPKQVDIMQ MCIIELKDRP GQPLFHLEHY IEGKYIKYNS NSGFVRDDNI 250
    RLTPQAFSHF TFERSGHQLI VVDIQGVGDL YTDPQIHTEK GTDFGDGNLG 300
    VRGMALFFYS HACNRICQSM GLTPFDLSPR EQDAVNQSTR LLQSAKTILR 350
    GTEEKCGSPR IRTLSSSRPP LLLRLSENSG DENMSDVTFD SLPSSPSSAT 400
    PHSQKLDHLH WPVFGDLDNM GPRDHDRMDN HRDSENSGDS GYPSEKRSDL 450
    DDPEPREHGH SNGNRRHESD EDSLGSSGRV CVETWNLLNP SRLHLPRPSA 500
    VALEVQRLNA LDLGRKIGKS VLGKVHLAMV RYHEGGRFCE KDEEWDRESA 550
    IFHLEHAADL GELEAIVGLG LMYSQLPHHI LADVSLKETE ENKTKGFDYL 600
    LKAAEAGDRH SMILVARAFD TGLNLSPDRC QDWSEALHWY NTALETTDCD 650
    EGGEYDGIQD EPQYALLARE AEMLLTGGFG LDKNPQRSGD LYTQAAEAAM 700
    EAMKGRLANQ YYEKAEEAWA QMEE 724
    Length:724
    Mass (Da):81,739
    Last modified:July 1, 1997 - v1
    Checksum:iB892D7D547C8E7AE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U93848 mRNA. Translation: AAB58271.1.
    BC003433 mRNA. Translation: AAH03433.1.
    CCDSiCCDS21796.1.
    RefSeqiNP_001254639.1. NM_001267710.1.
    NP_001254640.1. NM_001267711.1.
    NP_031934.1. NM_007908.4.
    XP_006507405.1. XM_006507342.1.
    XP_006507406.1. XM_006507343.1.
    UniGeneiMm.25997.
    Mm.470116.

    Genome annotation databases

    EnsembliENSMUST00000047875; ENSMUSP00000046595; ENSMUSG00000035064.
    ENSMUST00000106488; ENSMUSP00000102097; ENSMUSG00000035064.
    ENSMUST00000106489; ENSMUSP00000102098; ENSMUSG00000035064.
    GeneIDi13631.
    KEGGimmu:13631.
    UCSCiuc009jnb.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U93848 mRNA. Translation: AAB58271.1 .
    BC003433 mRNA. Translation: AAH03433.1 .
    CCDSi CCDS21796.1.
    RefSeqi NP_001254639.1. NM_001267710.1.
    NP_001254640.1. NM_001267711.1.
    NP_031934.1. NM_007908.4.
    XP_006507405.1. XM_006507342.1.
    XP_006507406.1. XM_006507343.1.
    UniGenei Mm.25997.
    Mm.470116.

    3D structure databases

    ProteinModelPortali O08796.
    SMRi O08796. Positions 116-322, 526-646.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O08796.

    Proteomic databases

    MaxQBi O08796.
    PaxDbi O08796.
    PRIDEi O08796.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047875 ; ENSMUSP00000046595 ; ENSMUSG00000035064 .
    ENSMUST00000106488 ; ENSMUSP00000102097 ; ENSMUSG00000035064 .
    ENSMUST00000106489 ; ENSMUSP00000102098 ; ENSMUSG00000035064 .
    GeneIDi 13631.
    KEGGi mmu:13631.
    UCSCi uc009jnb.2. mouse.

    Organism-specific databases

    CTDi 29904.
    MGIi MGI:1195261. Eef2k.

    Phylogenomic databases

    eggNOGi NOG145133.
    GeneTreei ENSGT00740000115539.
    HOGENOMi HOG000022023.
    HOVERGENi HBG002318.
    InParanoidi O08796.
    KOi K08292.
    OMAi QIHTETG.
    OrthoDBi EOG7673B0.
    PhylomeDBi O08796.
    TreeFami TF316085.

    Enzyme and pathway databases

    BRENDAi 2.7.11.20. 3474.

    Miscellaneous databases

    NextBioi 284316.
    PROi O08796.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O08796.
    Bgeei O08796.
    CleanExi MM_EEF2K.
    Genevestigatori O08796.

    Family and domain databases

    Gene3Di 1.25.40.10. 1 hit.
    InterProi IPR017400. Elongation_factor_2_kinase.
    IPR011009. Kinase-like_dom.
    IPR004166. MHCK_EF2_kinase.
    IPR006597. Sel1-like.
    IPR011990. TPR-like_helical.
    [Graphical view ]
    Pfami PF02816. Alpha_kinase. 1 hit.
    PF08238. Sel1. 4 hits.
    [Graphical view ]
    PIRSFi PIRSF038139. Elongation_factor_2_kinase. 1 hit.
    SMARTi SM00811. Alpha_kinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS51158. ALPHA_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION.
      Strain: BALB/c.
      Tissue: Spleen.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary gland.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiEF2K_MOUSE
    AccessioniPrimary (citable) accession number: O08796
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3