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O08796

- EF2K_MOUSE

UniProt

O08796 - EF2K_MOUSE

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Protein

Eukaryotic elongation factor 2 kinase

Gene
Eef2k
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.1 Publication

Catalytic activityi

ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.1 Publication

Enzyme regulationi

Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi295 – 3017ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium ion binding Source: InterPro
  3. calmodulin binding Source: UniProtKB
  4. elongation factor-2 kinase activity Source: UniProtKB
  5. translation factor activity, nucleic acid binding Source: UniProtKB

GO - Biological processi

  1. protein autophosphorylation Source: Ensembl
  2. regulation of protein autophosphorylation Source: UniProtKB
  3. translational elongation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.20. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic elongation factor 2 kinase (EC:2.7.11.20)
Short name:
eEF-2 kinase
Short name:
eEF-2K
Alternative name(s):
Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
Gene namesi
Name:Eef2k
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1195261. Eef2k.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 724723Eukaryotic elongation factor 2 kinasePRO_0000086937Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei61 – 611Phosphoserine; by autocatalysis
Modified residuei70 – 701Phosphoserine1 Publication
Modified residuei77 – 771Phosphoserine; by autocatalysis and TRPM7 By similarity
Modified residuei347 – 3471Phosphothreonine; by autocatalysis By similarity
Modified residuei352 – 3521Phosphothreonine; by autocatalysis By similarity
Modified residuei358 – 3581Phosphoserine; by MAPK13 and CDK1 By similarity
Modified residuei365 – 3651Phosphoserine; by autocatalysis, RPS6KA1 and RPS6KB1 By similarity
Modified residuei397 – 3971Phosphoserine; by AMPK By similarity
Modified residuei444 – 4441Phosphoserine; by autocatalysis By similarity
Modified residuei469 – 4691Phosphoserine By similarity
Modified residuei473 – 4731Phosphoserine; by autocatalysis
Modified residuei476 – 4761Phosphoserine By similarity
Modified residuei499 – 4991Phosphoserine; by PKA By similarity

Post-translational modificationi

Autophosphorylated at multiple residues, Thr-347 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-397 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-77 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-358 or RPS6KA1 and RPS6KB1 at Ser-365 instead decrease EEF2K activity and promote protein synthesis By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO08796.
PaxDbiO08796.
PRIDEiO08796.

PTM databases

PhosphoSiteiO08796.

Expressioni

Tissue specificityi

Ubiquitously expressed. Particularly abundant in skeletal muscle and heart.

Gene expression databases

ArrayExpressiO08796.
BgeeiO08796.
CleanExiMM_EEF2K.
GenevestigatoriO08796.

Interactioni

Subunit structurei

Monomer or homodimer Reviewed prediction.

Structurei

3D structure databases

ProteinModelPortaliO08796.
SMRiO08796. Positions 116-322, 526-646.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini115 – 325211Alpha-type protein kinaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni593 – 60917Calmodulin-binding Reviewed predictionAdd
BLAST
Regioni609 – 62618Pseudosubstrate/autoinhibitory domain Reviewed predictionAdd
BLAST

Domaini

The catalytic domain is located to N-terminal region. The neighbor region contains the calmodulin-binding domain By similarity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG145133.
GeneTreeiENSGT00740000115539.
HOGENOMiHOG000022023.
HOVERGENiHBG002318.
InParanoidiO08796.
KOiK08292.
OMAiQIHTETG.
OrthoDBiEOG7673B0.
PhylomeDBiO08796.
TreeFamiTF316085.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR017400. Elongation_factor_2_kinase.
IPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
IPR006597. Sel1-like.
IPR011990. TPR-like_helical.
[Graphical view]
PfamiPF02816. Alpha_kinase. 1 hit.
PF08238. Sel1. 4 hits.
[Graphical view]
PIRSFiPIRSF038139. Elongation_factor_2_kinase. 1 hit.
SMARTiSM00811. Alpha_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51158. ALPHA_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08796-1 [UniParc]FASTAAdd to Basket

« Hide

MADEDLIFCL EGVDGGRCSR AGHNADSDTD SDDDEGYFIC PITDDHMSNQ    50
NVSSKVQSYY SNLTKTECGS TGSPASSFHF KEAWKHAIEK AKHMPDPWAE 100
FHLEDIATEH ATRHRYNAVT GEWLKDEVLI KMASQPFGRG AMRECFRTKK 150
LSNFLHAQQW KGASNYVAKR YIEPVDRSVY FEDVQLQMEA KLWGEDYNRH 200
KPPKQVDIMQ MCIIELKDRP GQPLFHLEHY IEGKYIKYNS NSGFVRDDNI 250
RLTPQAFSHF TFERSGHQLI VVDIQGVGDL YTDPQIHTEK GTDFGDGNLG 300
VRGMALFFYS HACNRICQSM GLTPFDLSPR EQDAVNQSTR LLQSAKTILR 350
GTEEKCGSPR IRTLSSSRPP LLLRLSENSG DENMSDVTFD SLPSSPSSAT 400
PHSQKLDHLH WPVFGDLDNM GPRDHDRMDN HRDSENSGDS GYPSEKRSDL 450
DDPEPREHGH SNGNRRHESD EDSLGSSGRV CVETWNLLNP SRLHLPRPSA 500
VALEVQRLNA LDLGRKIGKS VLGKVHLAMV RYHEGGRFCE KDEEWDRESA 550
IFHLEHAADL GELEAIVGLG LMYSQLPHHI LADVSLKETE ENKTKGFDYL 600
LKAAEAGDRH SMILVARAFD TGLNLSPDRC QDWSEALHWY NTALETTDCD 650
EGGEYDGIQD EPQYALLARE AEMLLTGGFG LDKNPQRSGD LYTQAAEAAM 700
EAMKGRLANQ YYEKAEEAWA QMEE 724
Length:724
Mass (Da):81,739
Last modified:July 1, 1997 - v1
Checksum:iB892D7D547C8E7AE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U93848 mRNA. Translation: AAB58271.1.
BC003433 mRNA. Translation: AAH03433.1.
CCDSiCCDS21796.1.
RefSeqiNP_001254639.1. NM_001267710.1.
NP_001254640.1. NM_001267711.1.
NP_031934.1. NM_007908.4.
XP_006507405.1. XM_006507342.1.
XP_006507406.1. XM_006507343.1.
UniGeneiMm.25997.
Mm.470116.

Genome annotation databases

EnsembliENSMUST00000047875; ENSMUSP00000046595; ENSMUSG00000035064.
ENSMUST00000106488; ENSMUSP00000102097; ENSMUSG00000035064.
ENSMUST00000106489; ENSMUSP00000102098; ENSMUSG00000035064.
GeneIDi13631.
KEGGimmu:13631.
UCSCiuc009jnb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U93848 mRNA. Translation: AAB58271.1 .
BC003433 mRNA. Translation: AAH03433.1 .
CCDSi CCDS21796.1.
RefSeqi NP_001254639.1. NM_001267710.1.
NP_001254640.1. NM_001267711.1.
NP_031934.1. NM_007908.4.
XP_006507405.1. XM_006507342.1.
XP_006507406.1. XM_006507343.1.
UniGenei Mm.25997.
Mm.470116.

3D structure databases

ProteinModelPortali O08796.
SMRi O08796. Positions 116-322, 526-646.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei O08796.

Proteomic databases

MaxQBi O08796.
PaxDbi O08796.
PRIDEi O08796.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047875 ; ENSMUSP00000046595 ; ENSMUSG00000035064 .
ENSMUST00000106488 ; ENSMUSP00000102097 ; ENSMUSG00000035064 .
ENSMUST00000106489 ; ENSMUSP00000102098 ; ENSMUSG00000035064 .
GeneIDi 13631.
KEGGi mmu:13631.
UCSCi uc009jnb.2. mouse.

Organism-specific databases

CTDi 29904.
MGIi MGI:1195261. Eef2k.

Phylogenomic databases

eggNOGi NOG145133.
GeneTreei ENSGT00740000115539.
HOGENOMi HOG000022023.
HOVERGENi HBG002318.
InParanoidi O08796.
KOi K08292.
OMAi QIHTETG.
OrthoDBi EOG7673B0.
PhylomeDBi O08796.
TreeFami TF316085.

Enzyme and pathway databases

BRENDAi 2.7.11.20. 3474.

Miscellaneous databases

NextBioi 284316.
PROi O08796.
SOURCEi Search...

Gene expression databases

ArrayExpressi O08796.
Bgeei O08796.
CleanExi MM_EEF2K.
Genevestigatori O08796.

Family and domain databases

Gene3Di 1.25.40.10. 1 hit.
InterProi IPR017400. Elongation_factor_2_kinase.
IPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
IPR006597. Sel1-like.
IPR011990. TPR-like_helical.
[Graphical view ]
Pfami PF02816. Alpha_kinase. 1 hit.
PF08238. Sel1. 4 hits.
[Graphical view ]
PIRSFi PIRSF038139. Elongation_factor_2_kinase. 1 hit.
SMARTi SM00811. Alpha_kinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS51158. ALPHA_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION.
    Strain: BALB/c.
    Tissue: Spleen.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiEF2K_MOUSE
AccessioniPrimary (citable) accession number: O08796
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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