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Protein

Eukaryotic elongation factor 2 kinase

Gene

Eef2k

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.1 Publication

Catalytic activityi

ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.1 Publication

Enzyme regulationi

Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi295 – 301ATPBy similarity7

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium ion binding Source: InterPro
  • calmodulin binding Source: UniProtKB
  • elongation factor-2 kinase activity Source: UniProtKB
  • translation factor activity, RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.20. 3474.
ReactomeiR-MMU-166208. mTORC1-mediated signalling.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic elongation factor 2 kinase (EC:2.7.11.20)
Short name:
eEF-2 kinase
Short name:
eEF-2K
Alternative name(s):
Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
Gene namesi
Name:Eef2k
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1195261. Eef2k.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000869372 – 724Eukaryotic elongation factor 2 kinaseAdd BLAST723

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei27PhosphoserineBy similarity1
Modified residuei61Phosphoserine; by autocatalysisBy similarity1
Modified residuei70PhosphoserineCombined sources1
Modified residuei73PhosphoserineBy similarity1
Modified residuei77PhosphoserineCombined sources1
Modified residuei242PhosphoserineBy similarity1
Modified residuei347Phosphothreonine; by autocatalysisBy similarity1
Modified residuei352Phosphothreonine; by autocatalysisBy similarity1
Modified residuei358Phosphoserine; by MAPK13 and CDK1By similarity1
Modified residuei365Phosphoserine; by autocatalysis, RPS6KA1 and RPS6KB1By similarity1
Modified residuei391PhosphoserineCombined sources1
Modified residuei397Phosphoserine; by AMPKBy similarity1
Modified residuei434PhosphoserineBy similarity1
Modified residuei444PhosphoserineCombined sources1
Modified residuei469PhosphoserineBy similarity1
Modified residuei473Phosphoserine; by autocatalysisBy similarity1
Modified residuei476PhosphoserineBy similarity1
Modified residuei499Phosphoserine; by PKABy similarity1

Post-translational modificationi

Autophosphorylated at multiple residues, Thr-347 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-397 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-77 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-358 or RPS6KA1 and RPS6KB1 at Ser-365 instead decrease EEF2K activity and promote protein synthesis (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO08796.
MaxQBiO08796.
PaxDbiO08796.
PeptideAtlasiO08796.
PRIDEiO08796.

PTM databases

iPTMnetiO08796.
PhosphoSitePlusiO08796.

Expressioni

Tissue specificityi

Ubiquitously expressed. Particularly abundant in skeletal muscle and heart.

Gene expression databases

BgeeiENSMUSG00000035064.
CleanExiMM_EEF2K.
ExpressionAtlasiO08796. baseline and differential.
GenevisibleiO08796. MM.

Interactioni

Subunit structurei

Monomer or homodimer.Curated

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000046595.

Structurei

3D structure databases

ProteinModelPortaliO08796.
SMRiO08796.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini115 – 325Alpha-type protein kinasePROSITE-ProRule annotationAdd BLAST211

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni593 – 609Calmodulin-bindingSequence analysisAdd BLAST17
Regioni609 – 626Pseudosubstrate/autoinhibitory domainSequence analysisAdd BLAST18

Domaini

The catalytic domain is located to N-terminal region. The neighbor region contains the calmodulin-binding domain (By similarity).By similarity

Sequence similaritiesi

Contains 1 alpha-type protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IFRY. Eukaryota.
ENOG410YE3S. LUCA.
GeneTreeiENSGT00800000124135.
HOGENOMiHOG000022023.
HOVERGENiHBG002318.
InParanoidiO08796.
KOiK08292.
OMAiHEAGRFC.
OrthoDBiEOG091G09CK.
PhylomeDBiO08796.
TreeFamiTF316085.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR017400. eEF-2K.
IPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
IPR006597. Sel1-like.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF02816. Alpha_kinase. 1 hit.
PF08238. Sel1. 4 hits.
[Graphical view]
PIRSFiPIRSF038139. Elongation_factor_2_kinase. 1 hit.
SMARTiSM00811. Alpha_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51158. ALPHA_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08796-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADEDLIFCL EGVDGGRCSR AGHNADSDTD SDDDEGYFIC PITDDHMSNQ
60 70 80 90 100
NVSSKVQSYY SNLTKTECGS TGSPASSFHF KEAWKHAIEK AKHMPDPWAE
110 120 130 140 150
FHLEDIATEH ATRHRYNAVT GEWLKDEVLI KMASQPFGRG AMRECFRTKK
160 170 180 190 200
LSNFLHAQQW KGASNYVAKR YIEPVDRSVY FEDVQLQMEA KLWGEDYNRH
210 220 230 240 250
KPPKQVDIMQ MCIIELKDRP GQPLFHLEHY IEGKYIKYNS NSGFVRDDNI
260 270 280 290 300
RLTPQAFSHF TFERSGHQLI VVDIQGVGDL YTDPQIHTEK GTDFGDGNLG
310 320 330 340 350
VRGMALFFYS HACNRICQSM GLTPFDLSPR EQDAVNQSTR LLQSAKTILR
360 370 380 390 400
GTEEKCGSPR IRTLSSSRPP LLLRLSENSG DENMSDVTFD SLPSSPSSAT
410 420 430 440 450
PHSQKLDHLH WPVFGDLDNM GPRDHDRMDN HRDSENSGDS GYPSEKRSDL
460 470 480 490 500
DDPEPREHGH SNGNRRHESD EDSLGSSGRV CVETWNLLNP SRLHLPRPSA
510 520 530 540 550
VALEVQRLNA LDLGRKIGKS VLGKVHLAMV RYHEGGRFCE KDEEWDRESA
560 570 580 590 600
IFHLEHAADL GELEAIVGLG LMYSQLPHHI LADVSLKETE ENKTKGFDYL
610 620 630 640 650
LKAAEAGDRH SMILVARAFD TGLNLSPDRC QDWSEALHWY NTALETTDCD
660 670 680 690 700
EGGEYDGIQD EPQYALLARE AEMLLTGGFG LDKNPQRSGD LYTQAAEAAM
710 720
EAMKGRLANQ YYEKAEEAWA QMEE
Length:724
Mass (Da):81,739
Last modified:July 1, 1997 - v1
Checksum:iB892D7D547C8E7AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93848 mRNA. Translation: AAB58271.1.
BC003433 mRNA. Translation: AAH03433.1.
CCDSiCCDS21796.1.
RefSeqiNP_001254639.1. NM_001267710.1.
NP_001254640.1. NM_001267711.1.
NP_031934.1. NM_007908.4.
XP_006507405.1. XM_006507342.3.
XP_006507406.1. XM_006507343.3.
UniGeneiMm.25997.
Mm.470116.

Genome annotation databases

EnsembliENSMUST00000047875; ENSMUSP00000046595; ENSMUSG00000035064.
ENSMUST00000106488; ENSMUSP00000102097; ENSMUSG00000035064.
ENSMUST00000106489; ENSMUSP00000102098; ENSMUSG00000035064.
GeneIDi13631.
KEGGimmu:13631.
UCSCiuc009jnb.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93848 mRNA. Translation: AAB58271.1.
BC003433 mRNA. Translation: AAH03433.1.
CCDSiCCDS21796.1.
RefSeqiNP_001254639.1. NM_001267710.1.
NP_001254640.1. NM_001267711.1.
NP_031934.1. NM_007908.4.
XP_006507405.1. XM_006507342.3.
XP_006507406.1. XM_006507343.3.
UniGeneiMm.25997.
Mm.470116.

3D structure databases

ProteinModelPortaliO08796.
SMRiO08796.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000046595.

PTM databases

iPTMnetiO08796.
PhosphoSitePlusiO08796.

Proteomic databases

EPDiO08796.
MaxQBiO08796.
PaxDbiO08796.
PeptideAtlasiO08796.
PRIDEiO08796.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047875; ENSMUSP00000046595; ENSMUSG00000035064.
ENSMUST00000106488; ENSMUSP00000102097; ENSMUSG00000035064.
ENSMUST00000106489; ENSMUSP00000102098; ENSMUSG00000035064.
GeneIDi13631.
KEGGimmu:13631.
UCSCiuc009jnb.3. mouse.

Organism-specific databases

CTDi29904.
MGIiMGI:1195261. Eef2k.

Phylogenomic databases

eggNOGiENOG410IFRY. Eukaryota.
ENOG410YE3S. LUCA.
GeneTreeiENSGT00800000124135.
HOGENOMiHOG000022023.
HOVERGENiHBG002318.
InParanoidiO08796.
KOiK08292.
OMAiHEAGRFC.
OrthoDBiEOG091G09CK.
PhylomeDBiO08796.
TreeFamiTF316085.

Enzyme and pathway databases

BRENDAi2.7.11.20. 3474.
ReactomeiR-MMU-166208. mTORC1-mediated signalling.

Miscellaneous databases

PROiO08796.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035064.
CleanExiMM_EEF2K.
ExpressionAtlasiO08796. baseline and differential.
GenevisibleiO08796. MM.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR017400. eEF-2K.
IPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
IPR006597. Sel1-like.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF02816. Alpha_kinase. 1 hit.
PF08238. Sel1. 4 hits.
[Graphical view]
PIRSFiPIRSF038139. Elongation_factor_2_kinase. 1 hit.
SMARTiSM00811. Alpha_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51158. ALPHA_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF2K_MOUSE
AccessioniPrimary (citable) accession number: O08796
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.