Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glucosidase 2 subunit beta

Gene

Prkcsh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of glucosidase II.1 Publication

Pathwayi: N-glycan metabolism

This protein is involved in the pathway N-glycan metabolism, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway N-glycan metabolism and in Glycan metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi222 – 2331PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • in utero embryonic development Source: MGI
  • liver development Source: MGI
  • negative regulation of neuron projection development Source: MGI
  • N-glycan processing Source: MGI
  • nitrogen compound metabolic process Source: MGI
  • protein heterooligomerization Source: Ensembl
  • renal system development Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
R-MMU-901042. Calnexin/calreticulin cycle.
UniPathwayiUPA00957.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosidase 2 subunit beta
Alternative name(s):
80K-H protein
Glucosidase II subunit beta
Protein kinase C substrate 60.1 kDa protein heavy chain
Short name:
PKCSH
Gene namesi
Name:Prkcsh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:107877. Prkcsh.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 141 PublicationAdd BLAST14
ChainiPRO_000000414415 – 521Glucosidase 2 subunit betaAdd BLAST507

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineBy similarity1
Glycosylationi72N-linked (GlcNAc...)Sequence analysis1
Modified residuei89Phosphoserine; by PKCSequence analysis1
Modified residuei166N6-succinyllysineCombined sources1
Modified residuei168PhosphoserineBy similarity1
Modified residuei376Phosphoserine; by PKCSequence analysis1
Modified residuei383Phosphoserine; by PKCSequence analysis1
Modified residuei427Phosphoserine; by PKCSequence analysis1
Glycosylationi469N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO08795.
PeptideAtlasiO08795.
PRIDEiO08795.

PTM databases

iPTMnetiO08795.
PhosphoSitePlusiO08795.
SwissPalmiO08795.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003402.
CleanExiMM_PRKCSH.
ExpressionAtlasiO08795. baseline and differential.
GenevisibleiO08795. MM.

Interactioni

Subunit structurei

Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202370. 5 interactors.
IntActiO08795. 4 interactors.
MINTiMINT-1863550.
STRINGi10090.ENSMUSP00000003493.

Structurei

Secondary structure

1521
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi48 – 50Combined sources3
Beta strandi53 – 55Combined sources3
Beta strandi74 – 77Combined sources4
Beta strandi85 – 88Combined sources4
Helixi89 – 91Combined sources3
Beta strandi94 – 96Combined sources3
Beta strandi106 – 109Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5F0EX-ray1.74B30-117[»]
5H9OX-ray2.37B/D30-117[»]
5HJOX-ray2.29B/D35-117[»]
5HJRX-ray2.40B/D35-117[»]
5IEDX-ray1.81B30-117[»]
5IEEX-ray1.92B30-117[»]
5IEFX-ray2.38B30-117[»]
5IEGX-ray1.82B30-117[»]
ProteinModelPortaliO08795.
SMRiO08795.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini209 – 244EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini245 – 290EF-hand 2PROSITE-ProRule annotationAdd BLAST46
Domaini406 – 461PRKCSHAdd BLAST56

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi518 – 521Prevents secretion from ERPROSITE-ProRule annotation4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi285 – 390Glu/Pro-richAdd BLAST106

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 PRKCSH domain.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG2397. Eukaryota.
ENOG410XPE3. LUCA.
GeneTreeiENSGT00510000047770.
HOGENOMiHOG000007805.
HOVERGENiHBG051738.
InParanoidiO08795.
KOiK08288.
OMAiHEAEHDM.
OrthoDBiEOG091G06YU.
PhylomeDBiO08795.
TreeFamiTF329550.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.70.130.10. 1 hit.
4.10.400.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR026874. Glucosidase_2_bsu.
IPR002172. LDrepeatLR_classA_rpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR028146. PRKCSH_N.
[Graphical view]
PANTHERiPTHR12630:SF1. PTHR12630:SF1. 1 hit.
PfamiPF13202. EF-hand_5. 2 hits.
PF12999. PRKCSH-like. 1 hit.
PF13015. PRKCSH_1. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF50911. SSF50911. 1 hit.
SSF57424. SSF57424. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O08795-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLLLLLLLP LCWAVEVKRP RGVSLSNHHF YEESKPFTCL DGTATIPFDQ
60 70 80 90 100
VNDDYCDCKD GSDEPGTAAC PNGSFHCTNT GYKPLYILSS RVNDGVCDCC
110 120 130 140 150
DGTDEYNSGT VCENTCREKG RKEKESLQQL AEVTREGFRL KKILIEEWKT
160 170 180 190 200
AREEKQSKLL ELQAGKKSLE DQVETLRAAK EEAERPEKEA KDQHRKLWEE
210 220 230 240 250
QQAAAKARRE QERAASAFQE LDDNMDGMVS LAELQTHPEL DTDGDGALSE
260 270 280 290 300
EEAQALLSGD TQTDTTSFYD RVWAAIRDKY RSEVPPTDIP VPEETEPKEE
310 320 330 340 350
KPPVLPPTEE EEEEEEEPEE EEEEEEEEEE APPPLQPPQP PSPTEDEKMP
360 370 380 390 400
PYDEETQAII DAAQEARSKF EEVERSLKEM EESIRSLEQE ISFDFGPSGE
410 420 430 440 450
FAYLYSQCYE LTTNEYVYRL CPFKLVSQKP KHGGSPTSLG TWGSWAGPDH
460 470 480 490 500
DKFSAMKYEQ GTGCWQGPNR STTVRLLCGK ETVVTSTTEP SRCEYLMELM
510 520
TPAACPEPPP EAPSDGDHDE L
Length:521
Mass (Da):58,793
Last modified:July 1, 1997 - v1
Checksum:iBD070319898B4A38
GO
Isoform 2 (identifier: O08795-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     329-329: E → EVQGEQPK

Note: No experimental confirmation available.
Show »
Length:528
Mass (Da):59,560
Checksum:i253FD1A76A641713
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010672329E → EVQGEQPK in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92794 mRNA. Translation: AAC53183.1.
BC009816 mRNA. Translation: AAH09816.1.
CCDSiCCDS22918.1. [O08795-1]
CCDS80967.1. [O08795-2]
RefSeqiNP_001280579.1. NM_001293650.1. [O08795-2]
NP_001280580.1. NM_001293651.1. [O08795-1]
NP_032951.1. NM_008925.2. [O08795-1]
XP_006510158.1. XM_006510095.3. [O08795-2]
XP_006510159.1. XM_006510096.2. [O08795-2]
UniGeneiMm.214593.

Genome annotation databases

EnsembliENSMUST00000003493; ENSMUSP00000003493; ENSMUSG00000003402. [O08795-1]
ENSMUST00000115331; ENSMUSP00000110987; ENSMUSG00000003402. [O08795-2]
GeneIDi19089.
KEGGimmu:19089.
UCSCiuc009oni.2. mouse. [O08795-1]
uc009onk.2. mouse. [O08795-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92794 mRNA. Translation: AAC53183.1.
BC009816 mRNA. Translation: AAH09816.1.
CCDSiCCDS22918.1. [O08795-1]
CCDS80967.1. [O08795-2]
RefSeqiNP_001280579.1. NM_001293650.1. [O08795-2]
NP_001280580.1. NM_001293651.1. [O08795-1]
NP_032951.1. NM_008925.2. [O08795-1]
XP_006510158.1. XM_006510095.3. [O08795-2]
XP_006510159.1. XM_006510096.2. [O08795-2]
UniGeneiMm.214593.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5F0EX-ray1.74B30-117[»]
5H9OX-ray2.37B/D30-117[»]
5HJOX-ray2.29B/D35-117[»]
5HJRX-ray2.40B/D35-117[»]
5IEDX-ray1.81B30-117[»]
5IEEX-ray1.92B30-117[»]
5IEFX-ray2.38B30-117[»]
5IEGX-ray1.82B30-117[»]
ProteinModelPortaliO08795.
SMRiO08795.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202370. 5 interactors.
IntActiO08795. 4 interactors.
MINTiMINT-1863550.
STRINGi10090.ENSMUSP00000003493.

PTM databases

iPTMnetiO08795.
PhosphoSitePlusiO08795.
SwissPalmiO08795.

Proteomic databases

PaxDbiO08795.
PeptideAtlasiO08795.
PRIDEiO08795.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003493; ENSMUSP00000003493; ENSMUSG00000003402. [O08795-1]
ENSMUST00000115331; ENSMUSP00000110987; ENSMUSG00000003402. [O08795-2]
GeneIDi19089.
KEGGimmu:19089.
UCSCiuc009oni.2. mouse. [O08795-1]
uc009onk.2. mouse. [O08795-2]

Organism-specific databases

CTDi5589.
MGIiMGI:107877. Prkcsh.

Phylogenomic databases

eggNOGiKOG2397. Eukaryota.
ENOG410XPE3. LUCA.
GeneTreeiENSGT00510000047770.
HOGENOMiHOG000007805.
HOVERGENiHBG051738.
InParanoidiO08795.
KOiK08288.
OMAiHEAEHDM.
OrthoDBiEOG091G06YU.
PhylomeDBiO08795.
TreeFamiTF329550.

Enzyme and pathway databases

UniPathwayiUPA00957.
ReactomeiR-MMU-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
R-MMU-901042. Calnexin/calreticulin cycle.

Miscellaneous databases

ChiTaRSiPrkcsh. mouse.
PROiO08795.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003402.
CleanExiMM_PRKCSH.
ExpressionAtlasiO08795. baseline and differential.
GenevisibleiO08795. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.70.130.10. 1 hit.
4.10.400.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR026874. Glucosidase_2_bsu.
IPR002172. LDrepeatLR_classA_rpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR028146. PRKCSH_N.
[Graphical view]
PANTHERiPTHR12630:SF1. PTHR12630:SF1. 1 hit.
PfamiPF13202. EF-hand_5. 2 hits.
PF12999. PRKCSH-like. 1 hit.
PF13015. PRKCSH_1. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF50911. SSF50911. 1 hit.
SSF57424. SSF57424. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLU2B_MOUSE
AccessioniPrimary (citable) accession number: O08795
Secondary accession number(s): Q921X2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.