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O08795

- GLU2B_MOUSE

UniProt

O08795 - GLU2B_MOUSE

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Protein

Glucosidase 2 subunit beta

Gene

Prkcsh

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulatory subunit of glucosidase II.1 Publication

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi222 – 233121PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. RNA binding Source: MGI

GO - Biological processi

  1. in utero embryonic development Source: MGI
  2. liver development Source: MGI
  3. negative regulation of neuron projection development Source: MGI
  4. N-glycan processing Source: MGI
  5. nitrogen compound metabolic process Source: MGI
  6. protein heterooligomerization Source: Ensembl
  7. renal system development Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198616. Calnexin/calreticulin cycle.
REACT_198618. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
REACT_198647. Advanced glycosylation endproduct receptor signaling.
UniPathwayiUPA00957.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosidase 2 subunit beta
Alternative name(s):
80K-H protein
Glucosidase II subunit beta
Protein kinase C substrate 60.1 kDa protein heavy chain
Short name:
PKCSH
Gene namesi
Name:Prkcsh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:107877. Prkcsh.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 14141 PublicationAdd
BLAST
Chaini15 – 521507Glucosidase 2 subunit betaPRO_0000004144Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence Analysis
Modified residuei89 – 891Phosphoserine; by PKCSequence Analysis
Modified residuei166 – 1661N6-succinyllysine1 Publication
Modified residuei376 – 3761Phosphoserine; by PKCSequence Analysis
Modified residuei383 – 3831Phosphoserine; by PKCSequence Analysis
Modified residuei427 – 4271Phosphoserine; by PKCSequence Analysis
Glycosylationi469 – 4691N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO08795.
PaxDbiO08795.
PRIDEiO08795.

PTM databases

PhosphoSiteiO08795.

Expressioni

Gene expression databases

BgeeiO08795.
CleanExiMM_PRKCSH.
ExpressionAtlasiO08795. baseline.
GenevestigatoriO08795.

Interactioni

Subunit structurei

Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC.

Protein-protein interaction databases

BioGridi202370. 5 interactions.
IntActiO08795. 4 interactions.
MINTiMINT-1863550.

Structurei

3D structure databases

ProteinModelPortaliO08795.
SMRiO08795. Positions 33-107, 408-509.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini209 – 24436EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini245 – 29046EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini406 – 46156PRKCSHAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi518 – 5214Prevents secretion from ERPROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi285 – 390106Glu/Pro-richAdd
BLAST

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 PRKCSH domain.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG289998.
GeneTreeiENSGT00510000047770.
HOGENOMiHOG000007805.
HOVERGENiHBG051738.
InParanoidiO08795.
KOiK08288.
OMAiGQHCWNG.
OrthoDBiEOG7DZ8JX.
PhylomeDBiO08795.
TreeFamiTF329550.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.70.130.10. 2 hits.
4.10.400.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR026874. Glucosidase_2_bsu.
IPR002172. LDrepeatLR_classA_rpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR012913. PRKCSH.
IPR028146. PRKCSH_N.
[Graphical view]
PANTHERiPTHR12630:SF1. PTHR12630:SF1. 1 hit.
PfamiPF13202. EF-hand_5. 2 hits.
PF07915. PRKCSH. 1 hit.
PF12999. PRKCSH-like. 1 hit.
[Graphical view]
SMARTiSM00192. LDLa. 1 hit.
[Graphical view]
SUPFAMiSSF50911. SSF50911. 1 hit.
SSF57424. SSF57424. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O08795-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLLLLLLLP LCWAVEVKRP RGVSLSNHHF YEESKPFTCL DGTATIPFDQ
60 70 80 90 100
VNDDYCDCKD GSDEPGTAAC PNGSFHCTNT GYKPLYILSS RVNDGVCDCC
110 120 130 140 150
DGTDEYNSGT VCENTCREKG RKEKESLQQL AEVTREGFRL KKILIEEWKT
160 170 180 190 200
AREEKQSKLL ELQAGKKSLE DQVETLRAAK EEAERPEKEA KDQHRKLWEE
210 220 230 240 250
QQAAAKARRE QERAASAFQE LDDNMDGMVS LAELQTHPEL DTDGDGALSE
260 270 280 290 300
EEAQALLSGD TQTDTTSFYD RVWAAIRDKY RSEVPPTDIP VPEETEPKEE
310 320 330 340 350
KPPVLPPTEE EEEEEEEPEE EEEEEEEEEE APPPLQPPQP PSPTEDEKMP
360 370 380 390 400
PYDEETQAII DAAQEARSKF EEVERSLKEM EESIRSLEQE ISFDFGPSGE
410 420 430 440 450
FAYLYSQCYE LTTNEYVYRL CPFKLVSQKP KHGGSPTSLG TWGSWAGPDH
460 470 480 490 500
DKFSAMKYEQ GTGCWQGPNR STTVRLLCGK ETVVTSTTEP SRCEYLMELM
510 520
TPAACPEPPP EAPSDGDHDE L
Length:521
Mass (Da):58,793
Last modified:July 1, 1997 - v1
Checksum:iBD070319898B4A38
GO
Isoform 2 (identifier: O08795-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     329-329: E → EVQGEQPK

Note: No experimental confirmation available.

Show »
Length:528
Mass (Da):59,560
Checksum:i253FD1A76A641713
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei329 – 3291E → EVQGEQPK in isoform 2. 1 PublicationVSP_010672

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92794 mRNA. Translation: AAC53183.1.
BC009816 mRNA. Translation: AAH09816.1.
CCDSiCCDS22918.1. [O08795-1]
RefSeqiNP_001280579.1. NM_001293650.1. [O08795-2]
NP_001280580.1. NM_001293651.1. [O08795-1]
NP_032951.1. NM_008925.2. [O08795-1]
XP_006510158.1. XM_006510095.1. [O08795-2]
XP_006510159.1. XM_006510096.1. [O08795-2]
UniGeneiMm.214593.

Genome annotation databases

EnsembliENSMUST00000003493; ENSMUSP00000003493; ENSMUSG00000003402. [O08795-1]
ENSMUST00000115331; ENSMUSP00000110987; ENSMUSG00000003402. [O08795-2]
GeneIDi19089.
KEGGimmu:19089.
UCSCiuc009oni.1. mouse. [O08795-1]
uc009onk.1. mouse. [O08795-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92794 mRNA. Translation: AAC53183.1 .
BC009816 mRNA. Translation: AAH09816.1 .
CCDSi CCDS22918.1. [O08795-1 ]
RefSeqi NP_001280579.1. NM_001293650.1. [O08795-2 ]
NP_001280580.1. NM_001293651.1. [O08795-1 ]
NP_032951.1. NM_008925.2. [O08795-1 ]
XP_006510158.1. XM_006510095.1. [O08795-2 ]
XP_006510159.1. XM_006510096.1. [O08795-2 ]
UniGenei Mm.214593.

3D structure databases

ProteinModelPortali O08795.
SMRi O08795. Positions 33-107, 408-509.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202370. 5 interactions.
IntActi O08795. 4 interactions.
MINTi MINT-1863550.

PTM databases

PhosphoSitei O08795.

Proteomic databases

MaxQBi O08795.
PaxDbi O08795.
PRIDEi O08795.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000003493 ; ENSMUSP00000003493 ; ENSMUSG00000003402 . [O08795-1 ]
ENSMUST00000115331 ; ENSMUSP00000110987 ; ENSMUSG00000003402 . [O08795-2 ]
GeneIDi 19089.
KEGGi mmu:19089.
UCSCi uc009oni.1. mouse. [O08795-1 ]
uc009onk.1. mouse. [O08795-2 ]

Organism-specific databases

CTDi 5589.
MGIi MGI:107877. Prkcsh.

Phylogenomic databases

eggNOGi NOG289998.
GeneTreei ENSGT00510000047770.
HOGENOMi HOG000007805.
HOVERGENi HBG051738.
InParanoidi O08795.
KOi K08288.
OMAi GQHCWNG.
OrthoDBi EOG7DZ8JX.
PhylomeDBi O08795.
TreeFami TF329550.

Enzyme and pathway databases

UniPathwayi UPA00957 .
Reactomei REACT_198616. Calnexin/calreticulin cycle.
REACT_198618. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
REACT_198647. Advanced glycosylation endproduct receptor signaling.

Miscellaneous databases

ChiTaRSi Prkcsh. mouse.
NextBioi 295640.
PROi O08795.
SOURCEi Search...

Gene expression databases

Bgeei O08795.
CleanExi MM_PRKCSH.
ExpressionAtlasi O08795. baseline.
Genevestigatori O08795.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
2.70.130.10. 2 hits.
4.10.400.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR026874. Glucosidase_2_bsu.
IPR002172. LDrepeatLR_classA_rpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR012913. PRKCSH.
IPR028146. PRKCSH_N.
[Graphical view ]
PANTHERi PTHR12630:SF1. PTHR12630:SF1. 1 hit.
Pfami PF13202. EF-hand_5. 2 hits.
PF07915. PRKCSH. 1 hit.
PF12999. PRKCSH-like. 1 hit.
[Graphical view ]
SMARTi SM00192. LDLa. 1 hit.
[Graphical view ]
SUPFAMi SSF50911. SSF50911. 1 hit.
SSF57424. SSF57424. 1 hit.
PROSITEi PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the CD45-associated 116-kDa and 80-kDa proteins as the alpha- and beta-subunits of alpha-glucosidase II."
    Arendt C.W., Ostergaard H.L.
    J. Biol. Chem. 272:13117-13125(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 15-49 AND 462-466, FUNCTION, INTERACTION WITH GANAB AND PTPRC.
    Tissue: T-cell lymphoma.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Mammary tumor.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-166, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast and Liver.

Entry informationi

Entry nameiGLU2B_MOUSE
AccessioniPrimary (citable) accession number: O08795
Secondary accession number(s): Q921X2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 1, 1997
Last modified: November 26, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3