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O08795

- GLU2B_MOUSE

UniProt

O08795 - GLU2B_MOUSE

Protein

Glucosidase 2 subunit beta

Gene

Prkcsh

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Regulatory subunit of glucosidase II.1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi222 – 233121PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. protein binding Source: MGI
    3. RNA binding Source: MGI

    GO - Biological processi

    1. in utero embryonic development Source: MGI
    2. liver development Source: MGI
    3. negative regulation of neuron projection development Source: MGI
    4. N-glycan processing Source: MGI
    5. nitrogen compound metabolic process Source: MGI
    6. protein heterooligomerization Source: Ensembl
    7. renal system development Source: MGI

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198616. Calnexin/calreticulin cycle.
    REACT_198618. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
    REACT_198647. Advanced glycosylation endproduct receptor signaling.
    UniPathwayiUPA00957.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucosidase 2 subunit beta
    Alternative name(s):
    80K-H protein
    Glucosidase II subunit beta
    Protein kinase C substrate 60.1 kDa protein heavy chain
    Short name:
    PKCSH
    Gene namesi
    Name:Prkcsh
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:107877. Prkcsh.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: MGI

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 14141 PublicationAdd
    BLAST
    Chaini15 – 521507Glucosidase 2 subunit betaPRO_0000004144Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi72 – 721N-linked (GlcNAc...)Sequence Analysis
    Modified residuei89 – 891Phosphoserine; by PKCSequence Analysis
    Modified residuei166 – 1661N6-succinyllysine1 Publication
    Modified residuei376 – 3761Phosphoserine; by PKCSequence Analysis
    Modified residuei383 – 3831Phosphoserine; by PKCSequence Analysis
    Modified residuei427 – 4271Phosphoserine; by PKCSequence Analysis
    Glycosylationi469 – 4691N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiO08795.
    PaxDbiO08795.
    PRIDEiO08795.

    PTM databases

    PhosphoSiteiO08795.

    Expressioni

    Gene expression databases

    ArrayExpressiO08795.
    BgeeiO08795.
    CleanExiMM_PRKCSH.
    GenevestigatoriO08795.

    Interactioni

    Subunit structurei

    Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC.

    Protein-protein interaction databases

    BioGridi202370. 5 interactions.
    IntActiO08795. 4 interactions.
    MINTiMINT-1863550.

    Structurei

    3D structure databases

    ProteinModelPortaliO08795.
    SMRiO08795. Positions 33-107, 408-509.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini209 – 24436EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini245 – 29046EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini406 – 46156PRKCSHAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi518 – 5214Prevents secretion from ERPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi285 – 390106Glu/Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 PRKCSH domain.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG289998.
    GeneTreeiENSGT00510000047770.
    HOGENOMiHOG000007805.
    HOVERGENiHBG051738.
    KOiK08288.
    OMAiGQHCWNG.
    OrthoDBiEOG7DZ8JX.
    PhylomeDBiO08795.
    TreeFamiTF329550.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.70.130.10. 2 hits.
    4.10.400.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR026874. Glucosidase_2_bsu.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR009011. Man6P_isomerase_rcpt-bd_dom.
    IPR012913. PRKCSH.
    IPR028146. PRKCSH_N.
    [Graphical view]
    PANTHERiPTHR12630:SF1. PTHR12630:SF1. 1 hit.
    PfamiPF13202. EF-hand_5. 2 hits.
    PF07915. PRKCSH. 1 hit.
    PF12999. PRKCSH-like. 1 hit.
    [Graphical view]
    SMARTiSM00192. LDLa. 1 hit.
    [Graphical view]
    SUPFAMiSSF50911. SSF50911. 1 hit.
    SSF57424. SSF57424. 1 hit.
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O08795-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLLLLLLLLP LCWAVEVKRP RGVSLSNHHF YEESKPFTCL DGTATIPFDQ    50
    VNDDYCDCKD GSDEPGTAAC PNGSFHCTNT GYKPLYILSS RVNDGVCDCC 100
    DGTDEYNSGT VCENTCREKG RKEKESLQQL AEVTREGFRL KKILIEEWKT 150
    AREEKQSKLL ELQAGKKSLE DQVETLRAAK EEAERPEKEA KDQHRKLWEE 200
    QQAAAKARRE QERAASAFQE LDDNMDGMVS LAELQTHPEL DTDGDGALSE 250
    EEAQALLSGD TQTDTTSFYD RVWAAIRDKY RSEVPPTDIP VPEETEPKEE 300
    KPPVLPPTEE EEEEEEEPEE EEEEEEEEEE APPPLQPPQP PSPTEDEKMP 350
    PYDEETQAII DAAQEARSKF EEVERSLKEM EESIRSLEQE ISFDFGPSGE 400
    FAYLYSQCYE LTTNEYVYRL CPFKLVSQKP KHGGSPTSLG TWGSWAGPDH 450
    DKFSAMKYEQ GTGCWQGPNR STTVRLLCGK ETVVTSTTEP SRCEYLMELM 500
    TPAACPEPPP EAPSDGDHDE L 521
    Length:521
    Mass (Da):58,793
    Last modified:July 1, 1997 - v1
    Checksum:iBD070319898B4A38
    GO
    Isoform 2 (identifier: O08795-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         329-329: E → EVQGEQPK

    Note: No experimental confirmation available.

    Show »
    Length:528
    Mass (Da):59,560
    Checksum:i253FD1A76A641713
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei329 – 3291E → EVQGEQPK in isoform 2. 1 PublicationVSP_010672

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U92794 mRNA. Translation: AAC53183.1.
    BC009816 mRNA. Translation: AAH09816.1.
    CCDSiCCDS22918.1. [O08795-1]
    RefSeqiNP_032951.1. NM_008925.2. [O08795-1]
    XP_006510158.1. XM_006510095.1. [O08795-2]
    XP_006510159.1. XM_006510096.1. [O08795-2]
    UniGeneiMm.214593.

    Genome annotation databases

    EnsembliENSMUST00000003493; ENSMUSP00000003493; ENSMUSG00000003402. [O08795-1]
    ENSMUST00000115331; ENSMUSP00000110987; ENSMUSG00000003402. [O08795-2]
    GeneIDi19089.
    KEGGimmu:19089.
    UCSCiuc009oni.1. mouse. [O08795-1]
    uc009onk.1. mouse. [O08795-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U92794 mRNA. Translation: AAC53183.1 .
    BC009816 mRNA. Translation: AAH09816.1 .
    CCDSi CCDS22918.1. [O08795-1 ]
    RefSeqi NP_032951.1. NM_008925.2. [O08795-1 ]
    XP_006510158.1. XM_006510095.1. [O08795-2 ]
    XP_006510159.1. XM_006510096.1. [O08795-2 ]
    UniGenei Mm.214593.

    3D structure databases

    ProteinModelPortali O08795.
    SMRi O08795. Positions 33-107, 408-509.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202370. 5 interactions.
    IntActi O08795. 4 interactions.
    MINTi MINT-1863550.

    PTM databases

    PhosphoSitei O08795.

    Proteomic databases

    MaxQBi O08795.
    PaxDbi O08795.
    PRIDEi O08795.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000003493 ; ENSMUSP00000003493 ; ENSMUSG00000003402 . [O08795-1 ]
    ENSMUST00000115331 ; ENSMUSP00000110987 ; ENSMUSG00000003402 . [O08795-2 ]
    GeneIDi 19089.
    KEGGi mmu:19089.
    UCSCi uc009oni.1. mouse. [O08795-1 ]
    uc009onk.1. mouse. [O08795-2 ]

    Organism-specific databases

    CTDi 5589.
    MGIi MGI:107877. Prkcsh.

    Phylogenomic databases

    eggNOGi NOG289998.
    GeneTreei ENSGT00510000047770.
    HOGENOMi HOG000007805.
    HOVERGENi HBG051738.
    KOi K08288.
    OMAi GQHCWNG.
    OrthoDBi EOG7DZ8JX.
    PhylomeDBi O08795.
    TreeFami TF329550.

    Enzyme and pathway databases

    UniPathwayi UPA00957 .
    Reactomei REACT_198616. Calnexin/calreticulin cycle.
    REACT_198618. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
    REACT_198647. Advanced glycosylation endproduct receptor signaling.

    Miscellaneous databases

    ChiTaRSi PRKCSH. mouse.
    NextBioi 295640.
    PROi O08795.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O08795.
    Bgeei O08795.
    CleanExi MM_PRKCSH.
    Genevestigatori O08795.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.70.130.10. 2 hits.
    4.10.400.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR026874. Glucosidase_2_bsu.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR009011. Man6P_isomerase_rcpt-bd_dom.
    IPR012913. PRKCSH.
    IPR028146. PRKCSH_N.
    [Graphical view ]
    PANTHERi PTHR12630:SF1. PTHR12630:SF1. 1 hit.
    Pfami PF13202. EF-hand_5. 2 hits.
    PF07915. PRKCSH. 1 hit.
    PF12999. PRKCSH-like. 1 hit.
    [Graphical view ]
    SMARTi SM00192. LDLa. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50911. SSF50911. 1 hit.
    SSF57424. SSF57424. 1 hit.
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of the CD45-associated 116-kDa and 80-kDa proteins as the alpha- and beta-subunits of alpha-glucosidase II."
      Arendt C.W., Ostergaard H.L.
      J. Biol. Chem. 272:13117-13125(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 15-49 AND 462-466, FUNCTION, INTERACTION WITH GANAB AND PTPRC.
      Tissue: T-cell lymphoma.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Mammary tumor.
    3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-166, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast and Liver.

    Entry informationi

    Entry nameiGLU2B_MOUSE
    AccessioniPrimary (citable) accession number: O08795
    Secondary accession number(s): Q921X2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2004
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3