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O08795 (GLU2B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucosidase 2 subunit beta
Alternative name(s):
80K-H protein
Glucosidase II subunit beta
Protein kinase C substrate 60.1 kDa protein heavy chain
Short name=PKCSH
Gene names
Name:Prkcsh
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length521 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulatory subunit of glucosidase II. Ref.1

Pathway

Glycan metabolism; N-glycan metabolism.

Subunit structure

Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC.

Subcellular location

Endoplasmic reticulum.

Sequence similarities

Contains 2 EF-hand domains.

Contains 1 PRKCSH domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O08795-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O08795-2)

The sequence of this isoform differs from the canonical sequence as follows:
     329-329: E → EVQGEQPK
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1414 Ref.1
Chain15 – 521507Glucosidase 2 subunit beta
PRO_0000004144

Regions

Domain209 – 24436EF-hand 1
Domain245 – 29046EF-hand 2
Domain406 – 46156PRKCSH
Calcium binding222 – 233121 Potential
Motif518 – 5214Prevents secretion from ER Potential
Compositional bias285 – 390106Glu/Pro-rich

Amino acid modifications

Modified residue891Phosphoserine; by PKC Potential
Modified residue1661N6-succinyllysine Ref.3
Modified residue3761Phosphoserine; by PKC Potential
Modified residue3831Phosphoserine; by PKC Potential
Modified residue4271Phosphoserine; by PKC Potential
Glycosylation721N-linked (GlcNAc...) Potential
Glycosylation4691N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence3291E → EVQGEQPK in isoform 2.
VSP_010672

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: BD070319898B4A38

FASTA52158,793
        10         20         30         40         50         60 
MLLLLLLLLP LCWAVEVKRP RGVSLSNHHF YEESKPFTCL DGTATIPFDQ VNDDYCDCKD 

        70         80         90        100        110        120 
GSDEPGTAAC PNGSFHCTNT GYKPLYILSS RVNDGVCDCC DGTDEYNSGT VCENTCREKG 

       130        140        150        160        170        180 
RKEKESLQQL AEVTREGFRL KKILIEEWKT AREEKQSKLL ELQAGKKSLE DQVETLRAAK 

       190        200        210        220        230        240 
EEAERPEKEA KDQHRKLWEE QQAAAKARRE QERAASAFQE LDDNMDGMVS LAELQTHPEL 

       250        260        270        280        290        300 
DTDGDGALSE EEAQALLSGD TQTDTTSFYD RVWAAIRDKY RSEVPPTDIP VPEETEPKEE 

       310        320        330        340        350        360 
KPPVLPPTEE EEEEEEEPEE EEEEEEEEEE APPPLQPPQP PSPTEDEKMP PYDEETQAII 

       370        380        390        400        410        420 
DAAQEARSKF EEVERSLKEM EESIRSLEQE ISFDFGPSGE FAYLYSQCYE LTTNEYVYRL 

       430        440        450        460        470        480 
CPFKLVSQKP KHGGSPTSLG TWGSWAGPDH DKFSAMKYEQ GTGCWQGPNR STTVRLLCGK 

       490        500        510        520 
ETVVTSTTEP SRCEYLMELM TPAACPEPPP EAPSDGDHDE L 

« Hide

Isoform 2 [UniParc].

Checksum: 253FD1A76A641713
Show »

FASTA52859,560

References

« Hide 'large scale' references
[1]"Identification of the CD45-associated 116-kDa and 80-kDa proteins as the alpha- and beta-subunits of alpha-glucosidase II."
Arendt C.W., Ostergaard H.L.
J. Biol. Chem. 272:13117-13125(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 15-49 AND 462-466, FUNCTION, INTERACTION WITH GANAB AND PTPRC.
Tissue: T-cell lymphoma.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Mammary tumor.
[3]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-166, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast and Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U92794 mRNA. Translation: AAC53183.1.
BC009816 mRNA. Translation: AAH09816.1.
RefSeqNP_032951.1. NM_008925.1.
XP_006510157.1. XM_006510094.1.
XP_006510158.1. XM_006510095.1.
XP_006510159.1. XM_006510096.1.
XP_006510160.1. XM_006510097.1.
UniGeneMm.214593.

3D structure databases

ProteinModelPortalO08795.
SMRO08795. Positions 33-107, 408-509.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202370. 5 interactions.
IntActO08795. 4 interactions.
MINTMINT-1863550.

PTM databases

PhosphoSiteO08795.

Proteomic databases

PaxDbO08795.
PRIDEO08795.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000003493; ENSMUSP00000003493; ENSMUSG00000003402. [O08795-1]
ENSMUST00000115331; ENSMUSP00000110987; ENSMUSG00000003402. [O08795-2]
GeneID19089.
KEGGmmu:19089.
UCSCuc009oni.1. mouse. [O08795-1]
uc009onk.1. mouse. [O08795-2]

Organism-specific databases

CTD5589.
MGIMGI:107877. Prkcsh.

Phylogenomic databases

eggNOGNOG289998.
GeneTreeENSGT00510000047770.
HOGENOMHOG000007805.
HOVERGENHBG051738.
KOK08288.
OMAGQHCWNG.
OrthoDBEOG7DZ8JX.
PhylomeDBO08795.
TreeFamTF329550.

Enzyme and pathway databases

UniPathwayUPA00957.

Gene expression databases

ArrayExpressO08795.
BgeeO08795.
CleanExMM_PRKCSH.
GenevestigatorO08795.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
2.70.130.10. 2 hits.
4.10.400.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR026874. Glucosidase_2_bsu.
IPR002172. LDrepeatLR_classA_rpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR012913. PRKCSH.
IPR028146. PRKCSH_N.
[Graphical view]
PANTHERPTHR12630:SF1. PTHR12630:SF1. 1 hit.
PfamPF13202. EF-hand_5. 2 hits.
PF07915. PRKCSH. 1 hit.
PF12999. PRKCSH-like. 1 hit.
[Graphical view]
SMARTSM00192. LDLa. 1 hit.
[Graphical view]
SUPFAMSSF50911. SSF50911. 1 hit.
SSF57424. SSF57424. 1 hit.
PROSITEPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPRKCSH. mouse.
NextBio295640.
PROO08795.
SOURCESearch...

Entry information

Entry nameGLU2B_MOUSE
AccessionPrimary (citable) accession number: O08795
Secondary accession number(s): Q921X2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot