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Protein

Transcription factor COE2

Gene

Ebf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that, in osteoblasts, activates the decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast differentiation. Acts in synergy with the Wnt-responsive LEF1/CTNNB1 pathway. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri150 – 169C5-typeSequence analysisAdd BLAST20

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • protein dimerization activity Source: InterPro
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB

GO - Biological processi

  • adipose tissue development Source: MGI
  • brown fat cell differentiation Source: MGI
  • cell fate determination Source: MGI
  • positive regulation of chromatin binding Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: NTNU_SB

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor COE2
Alternative name(s):
Early B-cell factor 2
Short name:
EBF-2
Metencephalon-mesencephalon-olfactory transcription factor 1
Short name:
MET-mesencephalon-olfactory TF1
Short name:
MET-mesencephalon-olfactory transcription factor 1
Olf-1/EBF-like 3
Short name:
O/E-3
Short name:
OE-3
Gene namesi
Name:Ebf2
Synonyms:Coe2, Mmot1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:894332 Ebf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001078291 – 575Transcription factor COE2Add BLAST575

Proteomic databases

MaxQBiO08792
PaxDbiO08792
PeptideAtlasiO08792
PRIDEiO08792

PTM databases

iPTMnetiO08792
PhosphoSitePlusiO08792

Expressioni

Tissue specificityi

In adult expressed in olfactory epithelium and at a much lower level in Purkinje cells of the cerebellum. In embryo expressed in epithalamus, in cells near the ventricular zone of mesencephalon and on the ventral surface of rhombencephalon, in the developing vomeronasal organ, at a lower level in developing spinal cord. Not expressed in developing retina, inner ear, dorsal root ganglia, trigeminal ganglia and glossopharyngeal ganglia.

Developmental stagei

First detected at 9.0 dpc in the first and second archial arches. At 10.0 dpc and 10.5 dpc, expressed in somites, especially the forming sclerotomes. At 12.5 dpc, found in dorsal root ganglia. At 16.5 dpc, expressed in bone-forming areas and adipose tissues, as well as in specific neural tissues. In bone-forming areas, expressed along the mesenchymal condensation at 14.5 dpc, in the perichondrium and in cells invading the cartilagenous structures at 16.5 dpc. In 18.5 dpc tibias, scattered throughout the trabecular/cancellous bone area. In vitro, expression is induced during differentiation of immature osteoblasts, and then declines.1 Publication

Gene expression databases

BgeeiENSMUSG00000022053
CleanExiMM_EBF2
GenevisibleiO08792 MM

Interactioni

Subunit structurei

Forms either a homodimer or a heterodimer with a related family member.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei162Interaction with DNABy similarity1
Sitei171Interaction with DNABy similarity1

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022637

Structurei

3D structure databases

ProteinModelPortaliO08792
SMRiO08792
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini253 – 336IPT/TIGAdd BLAST84

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni62 – 65Interaction with DNABy similarity4
Regioni196 – 203Interaction with DNABy similarity8
Regioni235 – 238Interaction with DNABy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi453 – 534Pro/Ser/Thr-richAdd BLAST82

Sequence similaritiesi

Belongs to the COE family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri150 – 169C5-typeSequence analysisAdd BLAST20

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3836 Eukaryota
ENOG410XQ9Z LUCA
GeneTreeiENSGT00390000014051
HOGENOMiHOG000092311
HOVERGENiHBG005108
InParanoidiO08792
KOiK09103
OMAiEYATPCI
OrthoDBiEOG091G0A9D
PhylomeDBiO08792
TreeFamiTF313391

Family and domain databases

CDDicd01175 IPT_COE, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.3180, 1 hit
4.10.280.10, 2 hits
InterProiView protein in InterPro
IPR032200 COE_DBD
IPR038173 COE_DBD_sf
IPR032201 COE_HLH
IPR038006 COE_IPT
IPR036638 HLH_DNA-bd_sf
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
IPR003523 Transcription_factor_COE
IPR018350 Transcription_factor_COE_CS
PANTHERiPTHR10747 PTHR10747, 1 hit
PfamiView protein in Pfam
PF16422 COE1_DBD, 1 hit
PF16423 COE1_HLH, 1 hit
PF01833 TIG, 1 hit
SMARTiView protein in SMART
SM00429 IPT, 1 hit
SUPFAMiSSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS01345 COE, 1 hit

Sequencei

Sequence statusi: Complete.

O08792-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGIQDTLGR GPALKDKSLG AEMDSVRSWV RNVGVVDANV AAQSGVALSR
60 70 80 90 100
AHFEKQPPSN LRKSNFFHFV LALYDRQGQP VEIERTAFVD FVENDKEQGN
110 120 130 140 150
EKTNNGTHYK LQLLYSNGVR TEQDLYVRLI DSVTKQPIAY EGQNKNPEMC
160 170 180 190 200
RVLLTHEVMC SRCCEKKSCG NRNETPSDPV IIDRFFLKFF LKCNQNCLKT
210 220 230 240 250
AGNPRDMRRF QVVLSTTVNV DGHVLAVSDN MFVHNNSKHG RRARRLDPSE
260 270 280 290 300
ATPCIKAISP SEGWTTGGAM VIIIGDNFFD GLQVVFGTML VWSELITPHA
310 320 330 340 350
IRVQTPPRHI PGVVEVTLSY KSKQFCKGAP GRFIYTALNE PTIDYGFQRL
360 370 380 390 400
QKVIPRHPGD PERLAKEMLL KRAADLVEAL YGTPHNNQDI ILKRAADIAE
410 420 430 440 450
ALYSVPRNPS QIPALSSSPA HSGMMGINSY GSQLGVSISE STQGNNQGYI
460 470 480 490 500
RNTSSISPRG YSSSSTPQQS NYSTSSNSMN GYSNVPMANL GVPGSPGFLN
510 520 530 540 550
GSPTGSPYGI MSSSPTVGSS STSSILPFSS SVFPAVKQKS AFAPVIRPQG
560 570
SPSPACSSGN GNGFRAMTGL VVPPM
Length:575
Mass (Da):62,606
Last modified:May 30, 2000 - v4
Checksum:iE8CDD6DAC7637449
GO

Sequence cautioni

The sequence AAB58423 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37D → V in AAC64322 (PubMed:9389446).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71189 mRNA Translation: AAB58423.1 Different initiation.
U92703 mRNA Translation: AAB58323.1
U82441 mRNA Translation: AAC64322.1
AK053013 mRNA Translation: BAC35239.1
AK139262 mRNA Translation: BAE23936.1
BC049188 mRNA Translation: AAH49188.1
BC050922 mRNA Translation: AAH50922.1
CCDSiCCDS27228.1
RefSeqiNP_001263316.1, NM_001276387.1
NP_034225.1, NM_010095.6
XP_011243247.1, XM_011244945.2
UniGeneiMm.319947
Mm.470952

Genome annotation databases

EnsembliENSMUST00000022637; ENSMUSP00000022637; ENSMUSG00000022053
ENSMUST00000176029; ENSMUSP00000135782; ENSMUSG00000022053
ENSMUST00000176161; ENSMUSP00000135500; ENSMUSG00000022053
GeneIDi13592
KEGGimmu:13592
UCSCiuc007ukw.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCOE2_MOUSE
AccessioniPrimary (citable) accession number: O08792
Secondary accession number(s): Q543D5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: April 25, 2018
This is version 141 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health