Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Max-binding protein MNT

Gene

Mnt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: MGI
  3. RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
  4. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: MGI
  5. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. cell aging Source: MGI
  2. negative regulation of apoptotic signaling pathway Source: MGI
  3. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  4. regulation of cell cycle Source: MGI
  5. regulation of transcription, DNA-templated Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Max-binding protein MNT
Alternative name(s):
Myc antagonist MNT
Protein ROX
Gene namesi
Name:Mnt
Synonyms:Rox
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:109150. Mnt.

Subcellular locationi

GO - Cellular componenti

  1. nucleoplasm Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 591590Max-binding protein MNTPRO_0000127282Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO08789.
PRIDEiO08789.

PTM databases

PhosphoSiteiO08789.

Expressioni

Gene expression databases

BgeeiO08789.
CleanExiMM_MNT.
ExpressionAtlasiO08789. baseline and differential.
GenevestigatoriO08789.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer with MAX.

Protein-protein interaction databases

BioGridi201461. 3 interactions.
IntActiO08789. 1 interaction.
STRINGi10090.ENSMUSP00000000291.

Structurei

3D structure databases

ProteinModelPortaliO08789.
SMRiO08789. Positions 223-298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini222 – 27352bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni273 – 30129Leucine-zipperAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG314808.
GeneTreeiENSGT00510000048287.
HOGENOMiHOG000013115.
HOVERGENiHBG006379.
InParanoidiO08789.
KOiK09115.
OMAiTPPISHI.
OrthoDBiEOG74R1QW.
TreeFamiTF315654.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIETLLEAA RFLEWQAQQQ QRAREEQERL RLEREREREQ EQKRASNLAR
60 70 80 90 100
LAHALPVEEP RIEAPPLPLS PPAPPPAPPP PLATPAPLTV IPIPVVTNSP
110 120 130 140 150
QSLPPPPPLP PAAQPLPLAP RQPALVSTPG LSIKEPVTLP TRPQVPTPAP
160 170 180 190 200
LLPDAKTTVA PTGSPKPLQP LPAPILTIAP HPGVQPQLAP QQPPPPTLGT
210 220 230 240 250
LKLAPAEEAK SSEQKKRPGG IGTREVHNKL EKNRRAHLKE CFETLKRNIP
260 270 280 290 300
NVDDKKTSNL SVLRTALRYI QSLKRKEKEY EHEMERLARE KIATQQRLAE
310 320 330 340 350
LKHELSQWMD VLEIDRVLRQ TGQPEDDQAS TSTASEGEDN VDEEMEGDRA
360 370 380 390 400
GLGPPKLNHR PQPELLKSAL PTPSTAPAPL PTHPHPHPHP VALSPAHLPV
410 420 430 440 450
QQQQPPQQKT PLPAPPPPPA TPTQTLVPAP AHLVATAGGG STVIAHTATT
460 470 480 490 500
HASVIQTVNH VLQGPGGKHI AHIAPSAPSP AVQLAPATPP IGHITVHPAT
510 520 530 540 550
LNHVAHLGSQ LPLYPQPVAV SQPVAVSHIA HTLSHQQVNG TAGLGPPATV
560 570 580 590
MAKPAVGAQV VHHPQLVGQT VLNPVTMVTM PSFPVSTLKL A
Length:591
Mass (Da):63,274
Last modified:July 27, 2011 - v2
Checksum:i17C2B830381D2190
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti379 – 3791P → T in AAB38687 (PubMed:9000049).Curated
Sequence conflicti392 – 3921A → V in AAB38687 (PubMed:9000049).Curated
Sequence conflicti402 – 4032QQ → EE in AAB38687 (PubMed:9000049).Curated
Sequence conflicti414 – 4141A → G in AAB38687 (PubMed:9000049).Curated
Sequence conflicti431 – 4311A → V in AAB38687 (PubMed:9000049).Curated
Sequence conflicti465 – 4651P → A in AAB38687 (PubMed:9000049).Curated
Sequence conflicti525 – 5251A → T in AAB38687 (PubMed:9000049).Curated
Sequence conflicti558 – 5581A → G in AAB38687 (PubMed:9000049).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77356 mRNA. Translation: AAB38687.1.
Y07609 mRNA. Translation: CAA68878.1.
AL604066 Genomic DNA. Translation: CAI24245.1.
CH466596 Genomic DNA. Translation: EDL12780.1.
BC054534 mRNA. Translation: AAH54534.1.
CCDSiCCDS25037.1.
RefSeqiNP_034943.3. NM_010813.3.
UniGeneiMm.3759.

Genome annotation databases

EnsembliENSMUST00000000291; ENSMUSP00000000291; ENSMUSG00000000282.
GeneIDi17428.
KEGGimmu:17428.
UCSCiuc011xzb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77356 mRNA. Translation: AAB38687.1.
Y07609 mRNA. Translation: CAA68878.1.
AL604066 Genomic DNA. Translation: CAI24245.1.
CH466596 Genomic DNA. Translation: EDL12780.1.
BC054534 mRNA. Translation: AAH54534.1.
CCDSiCCDS25037.1.
RefSeqiNP_034943.3. NM_010813.3.
UniGeneiMm.3759.

3D structure databases

ProteinModelPortaliO08789.
SMRiO08789. Positions 223-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201461. 3 interactions.
IntActiO08789. 1 interaction.
STRINGi10090.ENSMUSP00000000291.

PTM databases

PhosphoSiteiO08789.

Proteomic databases

MaxQBiO08789.
PRIDEiO08789.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000291; ENSMUSP00000000291; ENSMUSG00000000282.
GeneIDi17428.
KEGGimmu:17428.
UCSCiuc011xzb.1. mouse.

Organism-specific databases

CTDi4335.
MGIiMGI:109150. Mnt.

Phylogenomic databases

eggNOGiNOG314808.
GeneTreeiENSGT00510000048287.
HOGENOMiHOG000013115.
HOVERGENiHBG006379.
InParanoidiO08789.
KOiK09115.
OMAiTPPISHI.
OrthoDBiEOG74R1QW.
TreeFamiTF315654.

Miscellaneous databases

NextBioi292056.
PROiO08789.
SOURCEiSearch...

Gene expression databases

BgeeiO08789.
CleanExiMM_MNT.
ExpressionAtlasiO08789. baseline and differential.
GenevestigatoriO08789.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mnt, a novel Max-interacting protein is coexpressed with Myc in proliferating cells and mediates repression at Myc binding sites."
    Hurlin P.J., Queva C., Eisenman R.N.
    Genes Dev. 11:44-58(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. "Rox, a novel bHLHZip protein expressed in quiescent cells that heterodimerizes with Max, binds a non-canonical E box and acts as a transcriptional repressor."
    Meroni G., Reymond A., Alcalay M., Borsani G., Tanigami A., Tonlorenzi R., Lo Nigro C., Messali S., Zollo M., Ledbetter D.H., Brent R., Ballabio A., Carrozzo R.
    EMBO J. 16:2892-2906(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiMNT_MOUSE
AccessioniPrimary (citable) accession number: O08789
Secondary accession number(s): P97349, Q6GTJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 27, 2011
Last modified: March 4, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.