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O08789

- MNT_MOUSE

UniProt

O08789 - MNT_MOUSE

Protein

Max-binding protein MNT

Gene

Mnt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: MGI
    3. protein binding Source: MGI
    4. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. cell aging Source: MGI
    2. negative regulation of apoptotic signaling pathway Source: MGI
    3. regulation of cell cycle Source: MGI
    4. regulation of transcription, DNA-templated Source: MGI
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Max-binding protein MNT
    Alternative name(s):
    Myc antagonist MNT
    Protein ROX
    Gene namesi
    Name:Mnt
    Synonyms:Rox
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:109150. Mnt.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 591590Max-binding protein MNTPRO_0000127282Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiO08789.

    PTM databases

    PhosphoSiteiO08789.

    Expressioni

    Gene expression databases

    ArrayExpressiO08789.
    BgeeiO08789.
    CleanExiMM_MNT.
    GenevestigatoriO08789.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer with MAX.

    Protein-protein interaction databases

    BioGridi201461. 3 interactions.
    IntActiO08789. 1 interaction.
    STRINGi10090.ENSMUSP00000000291.

    Structurei

    3D structure databases

    ProteinModelPortaliO08789.
    SMRiO08789. Positions 223-298.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini222 – 27352bHLHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni273 – 30129Leucine-zipperAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG314808.
    GeneTreeiENSGT00510000048287.
    HOGENOMiHOG000013115.
    HOVERGENiHBG006379.
    InParanoidiQ6GTJ3.
    KOiK09115.
    OMAiGPPKLNH.
    OrthoDBiEOG74R1QW.
    TreeFamiTF315654.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O08789-1 [UniParc]FASTAAdd to Basket

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    MSIETLLEAA RFLEWQAQQQ QRAREEQERL RLEREREREQ EQKRASNLAR    50
    LAHALPVEEP RIEAPPLPLS PPAPPPAPPP PLATPAPLTV IPIPVVTNSP 100
    QSLPPPPPLP PAAQPLPLAP RQPALVSTPG LSIKEPVTLP TRPQVPTPAP 150
    LLPDAKTTVA PTGSPKPLQP LPAPILTIAP HPGVQPQLAP QQPPPPTLGT 200
    LKLAPAEEAK SSEQKKRPGG IGTREVHNKL EKNRRAHLKE CFETLKRNIP 250
    NVDDKKTSNL SVLRTALRYI QSLKRKEKEY EHEMERLARE KIATQQRLAE 300
    LKHELSQWMD VLEIDRVLRQ TGQPEDDQAS TSTASEGEDN VDEEMEGDRA 350
    GLGPPKLNHR PQPELLKSAL PTPSTAPAPL PTHPHPHPHP VALSPAHLPV 400
    QQQQPPQQKT PLPAPPPPPA TPTQTLVPAP AHLVATAGGG STVIAHTATT 450
    HASVIQTVNH VLQGPGGKHI AHIAPSAPSP AVQLAPATPP IGHITVHPAT 500
    LNHVAHLGSQ LPLYPQPVAV SQPVAVSHIA HTLSHQQVNG TAGLGPPATV 550
    MAKPAVGAQV VHHPQLVGQT VLNPVTMVTM PSFPVSTLKL A 591
    Length:591
    Mass (Da):63,274
    Last modified:July 27, 2011 - v2
    Checksum:i17C2B830381D2190
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti379 – 3791P → T in AAB38687. (PubMed:9000049)Curated
    Sequence conflicti392 – 3921A → V in AAB38687. (PubMed:9000049)Curated
    Sequence conflicti402 – 4032QQ → EE in AAB38687. (PubMed:9000049)Curated
    Sequence conflicti414 – 4141A → G in AAB38687. (PubMed:9000049)Curated
    Sequence conflicti431 – 4311A → V in AAB38687. (PubMed:9000049)Curated
    Sequence conflicti465 – 4651P → A in AAB38687. (PubMed:9000049)Curated
    Sequence conflicti525 – 5251A → T in AAB38687. (PubMed:9000049)Curated
    Sequence conflicti558 – 5581A → G in AAB38687. (PubMed:9000049)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77356 mRNA. Translation: AAB38687.1.
    Y07609 mRNA. Translation: CAA68878.1.
    AL604066 Genomic DNA. Translation: CAI24245.1.
    CH466596 Genomic DNA. Translation: EDL12780.1.
    BC054534 mRNA. Translation: AAH54534.1.
    CCDSiCCDS25037.1.
    RefSeqiNP_034943.3. NM_010813.3.
    UniGeneiMm.3759.

    Genome annotation databases

    EnsembliENSMUST00000000291; ENSMUSP00000000291; ENSMUSG00000000282.
    GeneIDi17428.
    KEGGimmu:17428.
    UCSCiuc011xzb.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77356 mRNA. Translation: AAB38687.1 .
    Y07609 mRNA. Translation: CAA68878.1 .
    AL604066 Genomic DNA. Translation: CAI24245.1 .
    CH466596 Genomic DNA. Translation: EDL12780.1 .
    BC054534 mRNA. Translation: AAH54534.1 .
    CCDSi CCDS25037.1.
    RefSeqi NP_034943.3. NM_010813.3.
    UniGenei Mm.3759.

    3D structure databases

    ProteinModelPortali O08789.
    SMRi O08789. Positions 223-298.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201461. 3 interactions.
    IntActi O08789. 1 interaction.
    STRINGi 10090.ENSMUSP00000000291.

    PTM databases

    PhosphoSitei O08789.

    Proteomic databases

    PRIDEi O08789.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000000291 ; ENSMUSP00000000291 ; ENSMUSG00000000282 .
    GeneIDi 17428.
    KEGGi mmu:17428.
    UCSCi uc011xzb.1. mouse.

    Organism-specific databases

    CTDi 4335.
    MGIi MGI:109150. Mnt.

    Phylogenomic databases

    eggNOGi NOG314808.
    GeneTreei ENSGT00510000048287.
    HOGENOMi HOG000013115.
    HOVERGENi HBG006379.
    InParanoidi Q6GTJ3.
    KOi K09115.
    OMAi GPPKLNH.
    OrthoDBi EOG74R1QW.
    TreeFami TF315654.

    Miscellaneous databases

    NextBioi 292056.
    PROi O08789.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O08789.
    Bgeei O08789.
    CleanExi MM_MNT.
    Genevestigatori O08789.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mnt, a novel Max-interacting protein is coexpressed with Myc in proliferating cells and mediates repression at Myc binding sites."
      Hurlin P.J., Queva C., Eisenman R.N.
      Genes Dev. 11:44-58(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Embryo.
    2. "Rox, a novel bHLHZip protein expressed in quiescent cells that heterodimerizes with Max, binds a non-canonical E box and acts as a transcriptional repressor."
      Meroni G., Reymond A., Alcalay M., Borsani G., Tanigami A., Tonlorenzi R., Lo Nigro C., Messali S., Zollo M., Ledbetter D.H., Brent R., Ballabio A., Carrozzo R.
      EMBO J. 16:2892-2906(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Embryo.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiMNT_MOUSE
    AccessioniPrimary (citable) accession number: O08789
    Secondary accession number(s): P97349, Q6GTJ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3