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O08788

- DCTN1_MOUSE

UniProt

O08788 - DCTN1_MOUSE

Protein

Dynactin subunit 1

Gene

Dctn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.

    GO - Molecular functioni

    1. motor activity Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cytoplasmic microtubule organization Source: UniProtKB
    2. melanosome transport Source: MGI
    3. microtubule-based transport Source: InterPro
    4. positive regulation of intracellular protein transport Source: UniProtKB

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_106572. XBP1(S) activates chaperone genes.
    REACT_196550. MHC class II antigen presentation.
    REACT_196635. Regulation of PLK1 Activity at G2/M Transition.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dynactin subunit 1
    Alternative name(s):
    150 kDa dynein-associated polypeptide
    DAP-150
    Short name:
    DP-150
    p150-glued
    Gene namesi
    Name:Dctn1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:107745. Dctn1.

    Subcellular locationi

    Cytoplasm. Cytoplasmcytoskeleton
    Note: Colocalizes with microtubules.By similarity

    GO - Cellular componenti

    1. cell leading edge Source: MGI
    2. centrosome Source: UniProtKB
    3. cytoplasm Source: UniProtKB-SubCell
    4. dynactin complex Source: InterPro
    5. dynein complex Source: UniProtKB-KW
    6. kinetochore Source: Ensembl
    7. microtubule Source: UniProtKB-KW
    8. protein complex Source: MGI
    9. spindle pole Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Dynein, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12811281Dynactin subunit 1PRO_0000083519Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei108 – 1081PhosphothreonineBy similarity

    Post-translational modificationi

    Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiO08788.
    PaxDbiO08788.
    PRIDEiO08788.

    PTM databases

    PhosphoSiteiO08788.

    Expressioni

    Gene expression databases

    ArrayExpressiO08788.
    BgeeiO08788.
    CleanExiMM_DCTN1.
    GenevestigatoriO08788.

    Interactioni

    Subunit structurei

    Large macromolecular complex of at least 10 components; p150(glued) binds directly to microtubules and to cytoplasmic dynein. Interacts with the C-terminus of MAPRE1, MAPRE2 and MAPRE3. Interacts with FBXL5. Interacts with ECM29. Interacts with CLIP1. Interacts with CLN3 and DYNAP. Interacts with MISP; this interaction regulates its distribution at the cell cortex. Interacts with CEP131 By similarity. Interacts with SNX6.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Kif3aP287414EBI-776180,EBI-6169413
    Kif5aP331752EBI-776180,EBI-349710

    Protein-protein interaction databases

    BioGridi199072. 14 interactions.
    DIPiDIP-32057N.
    IntActiO08788. 24 interactions.
    MINTiMINT-1847327.

    Structurei

    3D structure databases

    ProteinModelPortaliO08788.
    SMRiO08788. Positions 25-98.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini48 – 9043CAP-GlyPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili214 – 547334Sequence AnalysisAdd
    BLAST
    Coiled coili943 – 1049107Sequence AnalysisAdd
    BLAST
    Coiled coili1185 – 121430Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi157 – 18428Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the dynactin 150 kDa subunit family.Curated
    Contains 1 CAP-Gly domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5244.
    GeneTreeiENSGT00740000115080.
    HOGENOMiHOG000015352.
    HOVERGENiHBG004956.
    InParanoidiO08788.
    KOiK04648.
    OMAiPEDSTMQ.
    TreeFamiTF105246.

    Family and domain databases

    Gene3Di2.30.30.190. 1 hit.
    InterProiIPR000938. CAP-Gly_domain.
    IPR027663. DCTN1.
    IPR022157. Dynactin.
    [Graphical view]
    PANTHERiPTHR18916:SF29. PTHR18916:SF29. 1 hit.
    PfamiPF01302. CAP_GLY. 1 hit.
    PF12455. Dynactin. 1 hit.
    [Graphical view]
    SMARTiSM01052. CAP_GLY. 1 hit.
    [Graphical view]
    SUPFAMiSSF74924. SSF74924. 1 hit.
    PROSITEiPS00845. CAP_GLY_1. 1 hit.
    PS50245. CAP_GLY_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O08788-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAQSRRHMSS RTPSGSRMST EASARPLRVG SRVEVIGKGH RGTVAYVGAT     50
    LFATGKWVGV ILDEAKGKND GTVQGRKYFT CDEGHGIFVR QSQIQVFEDG 100
    ADTTSPETPD SSASKVLKRE GADAAAKTSK LRGLKPKKAP TARKTTTRRP 150
    KPTRPASTGV AGPSSSLGPS GSASAGELSS SEPSTPAQTP LAAPIIPTPA 200
    LTSPGAAPPL PSPSKEEEGL RAQVRDLEEK LETLRLKRSE DKAKLKELEK 250
    HKIQLEQVQE WKSKMQEQQA DLQRRLKEAR KEAKEALEAK ERYMEEMADT 300
    ADAIEMATLD KEMAEERAES LQQEVEALKE RVDELTTDLE ILKAEIEEKG 350
    SDGAASSYQL KQLEEQNARL KDALVRMRDL SSSEKQEHVK LQKLMEKKNQ 400
    ELEVVRQQRE RLQEELSQAE STIDELKEQV DAALGAEEMV EMLTDRNLNL 450
    EEKVRELRET VGDLEAMNEM NDELQENARE TELELREQLD MAGARVREAQ 500
    KRVEAAQETV ADYQQTIKKY RQLTAHLQDV NRELTNQQEA SVERQQQPPP 550
    ETFDFKIKFA ETKAHAKAIE MELRQMEVAQ ANRHMSLLTA FMPDSFLRPG 600
    GDHDCVLVLL LMPRLICKAE LIRKQAQEKF DLSENCSERP GLRGAAGEQL 650
    SFAAGLVYSL SLLQATLHRY EHALSQCSVD VYKKVGSLYP EMSAHERSLD 700
    FLIELLHKDQ LDETVNVEPL TKAIKYYQHL YSIHLAEQPE DSTMQLADHI 750
    KFTQSALDCM GVEVGRLRAF LQGGQEATDI ALLLRDLETS CSDTRQFCKK 800
    IRRRMPGTDA PGIPAALAFG SQVSDTLLDC RKHLTWVVAV LQEVAAAAAQ 850
    LIAPLAENEG LPVAALEELA FKASEQIYGS PSSSPYECLR QSCTILISTM 900
    NKLATAMQEG EYDAERPPSK PPPVELRAAA LRAEITDAEG LGLKLEDRET 950
    VIKELKKSLK IKGEELSEAN VRLSLLEKKL DSAAKDADER IEKVQTRLDE 1000
    TQTLLRKKEK DFEETMDALQ ADIDQLEAEK AELKQRLNSQ SKRTIEGLRG 1050
    PPPSGIATLV SGIAGEEPQR GGAPGQAPGA LPGPGLVKDS PLLLQQISAM 1100
    RLHISQLQHE NSILRGAQMK ASLAALPPLH VAKLSLPPHE GPGGNLVAGA 1150
    LYRKTSQLLE KLNQLSTHTH VVDITRSSPA AKSPSAQLME QVAQLKSLSD 1200
    TIEKLKDEVL KETVTQRPGA TVPTDFATFP SSAFLRAKEE QQDDTVYMGK 1250
    VTFSCAAGLG QRHRLVLTQE QLHQLHSRLI S 1281
    Length:1,281
    Mass (Da):141,676
    Last modified:July 27, 2011 - v3
    Checksum:iA6CEDCAAA7022EB5
    GO
    Isoform 2 (identifier: O08788-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1049-1086: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,243
    Mass (Da):138,206
    Checksum:i17BD5B9ED7CD2474
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti406 – 4061R → W in BAE34241. (PubMed:16141072)Curated
    Sequence conflicti721 – 7233TKA → NKG in AAB57773. (PubMed:9070275)Curated
    Sequence conflicti732 – 7321S → R in AAB57773. (PubMed:9070275)Curated
    Sequence conflicti1202 – 12021I → V in BAE34241. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1049 – 108638Missing in isoform 2. 1 PublicationVSP_029584Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60312 mRNA. Translation: AAB57773.1.
    AK157867 mRNA. Translation: BAE34241.1.
    AC160400 Genomic DNA. No translation available.
    CCDSiCCDS39532.1. [O08788-1]
    PIRiJC5368.
    RefSeqiNP_031861.2. NM_007835.2. [O08788-1]
    UniGeneiMm.6919.

    Genome annotation databases

    EnsembliENSMUST00000113918; ENSMUSP00000109551; ENSMUSG00000031865. [O08788-2]
    ENSMUST00000113919; ENSMUSP00000109552; ENSMUSG00000031865. [O08788-1]
    GeneIDi13191.
    KEGGimmu:13191.
    UCSCiuc009cmz.2. mouse. [O08788-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60312 mRNA. Translation: AAB57773.1 .
    AK157867 mRNA. Translation: BAE34241.1 .
    AC160400 Genomic DNA. No translation available.
    CCDSi CCDS39532.1. [O08788-1 ]
    PIRi JC5368.
    RefSeqi NP_031861.2. NM_007835.2. [O08788-1 ]
    UniGenei Mm.6919.

    3D structure databases

    ProteinModelPortali O08788.
    SMRi O08788. Positions 25-98.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199072. 14 interactions.
    DIPi DIP-32057N.
    IntActi O08788. 24 interactions.
    MINTi MINT-1847327.

    Chemistry

    ChEMBLi CHEMBL2176787.

    PTM databases

    PhosphoSitei O08788.

    Proteomic databases

    MaxQBi O08788.
    PaxDbi O08788.
    PRIDEi O08788.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000113918 ; ENSMUSP00000109551 ; ENSMUSG00000031865 . [O08788-2 ]
    ENSMUST00000113919 ; ENSMUSP00000109552 ; ENSMUSG00000031865 . [O08788-1 ]
    GeneIDi 13191.
    KEGGi mmu:13191.
    UCSCi uc009cmz.2. mouse. [O08788-1 ]

    Organism-specific databases

    CTDi 1639.
    MGIi MGI:107745. Dctn1.

    Phylogenomic databases

    eggNOGi COG5244.
    GeneTreei ENSGT00740000115080.
    HOGENOMi HOG000015352.
    HOVERGENi HBG004956.
    InParanoidi O08788.
    KOi K04648.
    OMAi PEDSTMQ.
    TreeFami TF105246.

    Enzyme and pathway databases

    Reactomei REACT_106572. XBP1(S) activates chaperone genes.
    REACT_196550. MHC class II antigen presentation.
    REACT_196635. Regulation of PLK1 Activity at G2/M Transition.

    Miscellaneous databases

    ChiTaRSi DCTN1. mouse.
    NextBioi 283312.
    PROi O08788.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O08788.
    Bgeei O08788.
    CleanExi MM_DCTN1.
    Genevestigatori O08788.

    Family and domain databases

    Gene3Di 2.30.30.190. 1 hit.
    InterProi IPR000938. CAP-Gly_domain.
    IPR027663. DCTN1.
    IPR022157. Dynactin.
    [Graphical view ]
    PANTHERi PTHR18916:SF29. PTHR18916:SF29. 1 hit.
    Pfami PF01302. CAP_GLY. 1 hit.
    PF12455. Dynactin. 1 hit.
    [Graphical view ]
    SMARTi SM01052. CAP_GLY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74924. SSF74924. 1 hit.
    PROSITEi PS00845. CAP_GLY_1. 1 hit.
    PS50245. CAP_GLY_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse p150Glued (dynactin 1) cDNA sequence and evaluation as a candidate for the neuromuscular disease mutation mnd2."
      Jang W., Weber J.S., Tokito M.K., Holzbaur E.L., Meisler M.H.
      Biochem. Biophys. Res. Commun. 231:344-347(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Inner ear.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport."
      Hong Z., Yang Y., Zhang C., Niu Y., Li K., Zhao X., Liu J.J.
      Cell Res. 19:1334-1349(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNX6.

    Entry informationi

    Entry nameiDCTN1_MOUSE
    AccessioniPrimary (citable) accession number: O08788
    Secondary accession number(s): E9QLJ1, Q3TZG7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 110 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3