O08788 (DCTN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dynactin subunit 1

Alternative name(s):
150 kDa dynein-associated polypeptide
DAP-150
Short name=DP-150
p150-glued

Gene names

Name:

Dctn1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1281 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.
Subunit structure	Large macromolecular complex of at least 10 components; p150(glued) binds directly to microtubules and to cytoplasmic dynein. Interacts with the C-terminus of MAPRE1, MAPRE2 and MAPRE3. Interacts with FBXL5 By similarity. Interacts with ECM29. Interacts with CLIP1 By similarity. Interacts with SNX6. Interacts with CLN3 and DYNAP By similarity. Ref.5
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton. Note: Colocalizes with microtubules By similarity.
Post-translational modification	Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome By similarity.
Sequence similarities	Belongs to the dynactin 150 kDa subunit family. Contains 1 CAP-Gly domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm Cytoskeleton Dynein Microtubule
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell leading edge Inferred from direct assay PubMed 18331715. Source: MGI centrosome Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell dynein complex Inferred from electronic annotation. Source: UniProtKB-KW kinetochore Inferred from electronic annotation. Source: Compara microtubule Inferred from electronic annotation. Source: UniProtKB-KW spindle pole Inferred from electronic annotation. Source: Compara
Molecular_function	motor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O08788-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O08788-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1049-1086: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1281	1281	Dynactin subunit 1		PRO_0000083519
Regions
	Domain	48 – 90	43	CAP-Gly
	Coiled coil	214 – 547	334	Potential
	Coiled coil	943 – 1049	107	Potential
	Coiled coil	1185 – 1214	30	Potential
	Compositional bias	157 – 184	28	Ser-rich
Amino acid modifications
	Modified residue	108	1	Phosphothreonine By similarity
	Modified residue	541	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	1049 – 1086	38	Missing in isoform 2.		VSP_029584
Experimental info
	Sequence conflict	406	1	R → W in BAE34241. Ref.2
	Sequence conflict	721 – 723	3	TKA → NKG in AAB57773. Ref.1
	Sequence conflict	732	1	S → R in AAB57773. Ref.1
	Sequence conflict	1202	1	I → V in BAE34241. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: A6CEDCAAA7022EB5	FASTA	1,281	141,676
	10 20 30 40 50 60 MAQSRRHMSS RTPSGSRMST EASARPLRVG SRVEVIGKGH RGTVAYVGAT LFATGKWVGV 70 80 90 100 110 120 ILDEAKGKND GTVQGRKYFT CDEGHGIFVR QSQIQVFEDG ADTTSPETPD SSASKVLKRE 130 140 150 160 170 180 GADAAAKTSK LRGLKPKKAP TARKTTTRRP KPTRPASTGV AGPSSSLGPS GSASAGELSS 190 200 210 220 230 240 SEPSTPAQTP LAAPIIPTPA LTSPGAAPPL PSPSKEEEGL RAQVRDLEEK LETLRLKRSE 250 260 270 280 290 300 DKAKLKELEK HKIQLEQVQE WKSKMQEQQA DLQRRLKEAR KEAKEALEAK ERYMEEMADT 310 320 330 340 350 360 ADAIEMATLD KEMAEERAES LQQEVEALKE RVDELTTDLE ILKAEIEEKG SDGAASSYQL 370 380 390 400 410 420 KQLEEQNARL KDALVRMRDL SSSEKQEHVK LQKLMEKKNQ ELEVVRQQRE RLQEELSQAE 430 440 450 460 470 480 STIDELKEQV DAALGAEEMV EMLTDRNLNL EEKVRELRET VGDLEAMNEM NDELQENARE 490 500 510 520 530 540 TELELREQLD MAGARVREAQ KRVEAAQETV ADYQQTIKKY RQLTAHLQDV NRELTNQQEA 550 560 570 580 590 600 SVERQQQPPP ETFDFKIKFA ETKAHAKAIE MELRQMEVAQ ANRHMSLLTA FMPDSFLRPG 610 620 630 640 650 660 GDHDCVLVLL LMPRLICKAE LIRKQAQEKF DLSENCSERP GLRGAAGEQL SFAAGLVYSL 670 680 690 700 710 720 SLLQATLHRY EHALSQCSVD VYKKVGSLYP EMSAHERSLD FLIELLHKDQ LDETVNVEPL 730 740 750 760 770 780 TKAIKYYQHL YSIHLAEQPE DSTMQLADHI KFTQSALDCM GVEVGRLRAF LQGGQEATDI 790 800 810 820 830 840 ALLLRDLETS CSDTRQFCKK IRRRMPGTDA PGIPAALAFG SQVSDTLLDC RKHLTWVVAV 850 860 870 880 890 900 LQEVAAAAAQ LIAPLAENEG LPVAALEELA FKASEQIYGS PSSSPYECLR QSCTILISTM 910 920 930 940 950 960 NKLATAMQEG EYDAERPPSK PPPVELRAAA LRAEITDAEG LGLKLEDRET VIKELKKSLK 970 980 990 1000 1010 1020 IKGEELSEAN VRLSLLEKKL DSAAKDADER IEKVQTRLDE TQTLLRKKEK DFEETMDALQ 1030 1040 1050 1060 1070 1080 ADIDQLEAEK AELKQRLNSQ SKRTIEGLRG PPPSGIATLV SGIAGEEPQR GGAPGQAPGA 1090 1100 1110 1120 1130 1140 LPGPGLVKDS PLLLQQISAM RLHISQLQHE NSILRGAQMK ASLAALPPLH VAKLSLPPHE 1150 1160 1170 1180 1190 1200 GPGGNLVAGA LYRKTSQLLE KLNQLSTHTH VVDITRSSPA AKSPSAQLME QVAQLKSLSD 1210 1220 1230 1240 1250 1260 TIEKLKDEVL KETVTQRPGA TVPTDFATFP SSAFLRAKEE QQDDTVYMGK VTFSCAAGLG 1270 1280 QRHRLVLTQE QLHQLHSRLI S
	Isoform 2 [UniParc]. Checksum: 17BD5B9ED7CD2474	FASTA	1,243	138,206
	10 20 30 40 50 60 MAQSRRHMSS RTPSGSRMST EASARPLRVG SRVEVIGKGH RGTVAYVGAT LFATGKWVGV 70 80 90 100 110 120 ILDEAKGKND GTVQGRKYFT CDEGHGIFVR QSQIQVFEDG ADTTSPETPD SSASKVLKRE 130 140 150 160 170 180 GADAAAKTSK LRGLKPKKAP TARKTTTRRP KPTRPASTGV AGPSSSLGPS GSASAGELSS 190 200 210 220 230 240 SEPSTPAQTP LAAPIIPTPA LTSPGAAPPL PSPSKEEEGL RAQVRDLEEK LETLRLKRSE 250 260 270 280 290 300 DKAKLKELEK HKIQLEQVQE WKSKMQEQQA DLQRRLKEAR KEAKEALEAK ERYMEEMADT 310 320 330 340 350 360 ADAIEMATLD KEMAEERAES LQQEVEALKE RVDELTTDLE ILKAEIEEKG SDGAASSYQL 370 380 390 400 410 420 KQLEEQNARL KDALVRMRDL SSSEKQEHVK LQKLMEKKNQ ELEVVRQQRE RLQEELSQAE 430 440 450 460 470 480 STIDELKEQV DAALGAEEMV EMLTDRNLNL EEKVRELRET VGDLEAMNEM NDELQENARE 490 500 510 520 530 540 TELELREQLD MAGARVREAQ KRVEAAQETV ADYQQTIKKY RQLTAHLQDV NRELTNQQEA 550 560 570 580 590 600 SVERQQQPPP ETFDFKIKFA ETKAHAKAIE MELRQMEVAQ ANRHMSLLTA FMPDSFLRPG 610 620 630 640 650 660 GDHDCVLVLL LMPRLICKAE LIRKQAQEKF DLSENCSERP GLRGAAGEQL SFAAGLVYSL 670 680 690 700 710 720 SLLQATLHRY EHALSQCSVD VYKKVGSLYP EMSAHERSLD FLIELLHKDQ LDETVNVEPL 730 740 750 760 770 780 TKAIKYYQHL YSIHLAEQPE DSTMQLADHI KFTQSALDCM GVEVGRLRAF LQGGQEATDI 790 800 810 820 830 840 ALLLRDLETS CSDTRQFCKK IRRRMPGTDA PGIPAALAFG SQVSDTLLDC RKHLTWVVAV 850 860 870 880 890 900 LQEVAAAAAQ LIAPLAENEG LPVAALEELA FKASEQIYGS PSSSPYECLR QSCTILISTM 910 920 930 940 950 960 NKLATAMQEG EYDAERPPSK PPPVELRAAA LRAEITDAEG LGLKLEDRET VIKELKKSLK 970 980 990 1000 1010 1020 IKGEELSEAN VRLSLLEKKL DSAAKDADER IEKVQTRLDE TQTLLRKKEK DFEETMDALQ 1030 1040 1050 1060 1070 1080 ADIDQLEAEK AELKQRLNSQ SKRTIEGLVK DSPLLLQQIS AMRLHISQLQ HENSILRGAQ 1090 1100 1110 1120 1130 1140 MKASLAALPP LHVAKLSLPP HEGPGGNLVA GALYRKTSQL LEKLNQLSTH THVVDITRSS 1150 1160 1170 1180 1190 1200 PAAKSPSAQL MEQVAQLKSL SDTIEKLKDE VLKETVTQRP GATVPTDFAT FPSSAFLRAK 1210 1220 1230 1240 EEQQDDTVYM GKVTFSCAAG LGQRHRLVLT QEQLHQLHSR LIS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse p150Glued (dynactin 1) cDNA sequence and evaluation as a candidate for the neuromuscular disease mutation mnd2." Jang W., Weber J.S., Tokito M.K., Holzbaur E.L., Meisler M.H. Biochem. Biophys. Res. Commun. 231:344-347(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Inner ear.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-541, MASS SPECTROMETRY. Tissue: Liver.
[5]	"The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport." Hong Z., Yang Y., Zhang C., Niu Y., Li K., Zhao X., Liu J.J. Cell Res. 19:1334-1349(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SNX6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U60312 mRNA. Translation: AAB57773.1. AK157867 mRNA. Translation: BAE34241.1. AC160400 Genomic DNA. No translation available.
IPI	IPI00115663. IPI00857569.
PIR	JC5368.
RefSeq	NP_031861.2. NM_007835.2.
UniGene	Mm.6919.
3D structure databases
ProteinModelPortal	O08788.
SMR	O08788. Positions 25-98.
ModBase	Search...
Protein-protein interaction databases
IntAct	O08788. 13 interactions.
MINT	MINT-1847327.
PTM databases
PhosphoSite	O08788.
Proteomic databases
PaxDb	O08788.
PRIDE	O08788.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000113918; ENSMUSP00000109551; ENSMUSG00000031865. ENSMUST00000113919; ENSMUSP00000109552; ENSMUSG00000031865.
GeneID	13191.
KEGG	mmu:13191.
Organism-specific databases
CTD	1639.
MGI	MGI:107745. Dctn1.
Phylogenomic databases
eggNOG	COG5244.
GeneTree	ENSGT00700000104055.
HOGENOM	HOG000015352.
HOVERGEN	HBG004956.
InParanoid	O08788.
KO	K04648.
OMA	HVYSRTP.
Gene expression databases
ArrayExpress	O08788.
Bgee	O08788.
CleanEx	MM_DCTN1.
Genevestigator	O08788.
GermOnline	ENSMUSG00000031865. Mus musculus.
Family and domain databases
Gene3D	2.30.30.190. 1 hit.
InterPro	IPR000938. CAP-Gly_domain. IPR022157. Dynactin. [Graphical view]
Pfam	PF01302. CAP_GLY. 1 hit. PF12455. Dynactin. 1 hit. [Graphical view]
SMART	SM01052. CAP_GLY. 1 hit. [Graphical view]
SUPFAM	SSF74924. CAP-Gly_domain. 1 hit.
PROSITE	PS00845. CAP_GLY_1. 1 hit. PS50245. CAP_GLY_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	DCTN1. mouse.
NextBio	283312.
SOURCE	Search...

Entry information

Entry name

DCTN1_MOUSE

Accession

Primary (citable) accession number: O08788
Secondary accession number(s): E9QLJ1, Q3TZG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program