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O08788 (DCTN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dynactin subunit 1
Alternative name(s):
150 kDa dynein-associated polypeptide
DAP-150
Short name=DP-150
p150-glued
Gene names
Name:Dctn1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1281 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.

Subunit structure

Large macromolecular complex of at least 10 components; p150(glued) binds directly to microtubules and to cytoplasmic dynein. Interacts with the C-terminus of MAPRE1, MAPRE2 and MAPRE3. Interacts with FBXL5. Interacts with ECM29. Interacts with CLIP1. Interacts with CLN3 and DYNAP. Interacts with MISP; this interaction regulates its distribution at the cell cortex. Interacts with CEP131 By similarity. Interacts with SNX6. Ref.4

Subcellular location

Cytoplasm. Cytoplasmcytoskeleton. Note: Colocalizes with microtubules By similarity.

Post-translational modification

Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome By similarity.

Sequence similarities

Belongs to the dynactin 150 kDa subunit family.

Contains 1 CAP-Gly domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Kif3aP287414EBI-776180,EBI-6169413
Kif5aP331752EBI-776180,EBI-349710

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O08788-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O08788-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1049-1086: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12811281Dynactin subunit 1
PRO_0000083519

Regions

Domain48 – 9043CAP-Gly
Coiled coil214 – 547334 Potential
Coiled coil943 – 1049107 Potential
Coiled coil1185 – 121430 Potential
Compositional bias157 – 18428Ser-rich

Amino acid modifications

Modified residue1081Phosphothreonine By similarity

Natural variations

Alternative sequence1049 – 108638Missing in isoform 2.
VSP_029584

Experimental info

Sequence conflict4061R → W in BAE34241. Ref.2
Sequence conflict721 – 7233TKA → NKG in AAB57773. Ref.1
Sequence conflict7321S → R in AAB57773. Ref.1
Sequence conflict12021I → V in BAE34241. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: A6CEDCAAA7022EB5

FASTA1,281141,676
        10         20         30         40         50         60 
MAQSRRHMSS RTPSGSRMST EASARPLRVG SRVEVIGKGH RGTVAYVGAT LFATGKWVGV 

        70         80         90        100        110        120 
ILDEAKGKND GTVQGRKYFT CDEGHGIFVR QSQIQVFEDG ADTTSPETPD SSASKVLKRE 

       130        140        150        160        170        180 
GADAAAKTSK LRGLKPKKAP TARKTTTRRP KPTRPASTGV AGPSSSLGPS GSASAGELSS 

       190        200        210        220        230        240 
SEPSTPAQTP LAAPIIPTPA LTSPGAAPPL PSPSKEEEGL RAQVRDLEEK LETLRLKRSE 

       250        260        270        280        290        300 
DKAKLKELEK HKIQLEQVQE WKSKMQEQQA DLQRRLKEAR KEAKEALEAK ERYMEEMADT 

       310        320        330        340        350        360 
ADAIEMATLD KEMAEERAES LQQEVEALKE RVDELTTDLE ILKAEIEEKG SDGAASSYQL 

       370        380        390        400        410        420 
KQLEEQNARL KDALVRMRDL SSSEKQEHVK LQKLMEKKNQ ELEVVRQQRE RLQEELSQAE 

       430        440        450        460        470        480 
STIDELKEQV DAALGAEEMV EMLTDRNLNL EEKVRELRET VGDLEAMNEM NDELQENARE 

       490        500        510        520        530        540 
TELELREQLD MAGARVREAQ KRVEAAQETV ADYQQTIKKY RQLTAHLQDV NRELTNQQEA 

       550        560        570        580        590        600 
SVERQQQPPP ETFDFKIKFA ETKAHAKAIE MELRQMEVAQ ANRHMSLLTA FMPDSFLRPG 

       610        620        630        640        650        660 
GDHDCVLVLL LMPRLICKAE LIRKQAQEKF DLSENCSERP GLRGAAGEQL SFAAGLVYSL 

       670        680        690        700        710        720 
SLLQATLHRY EHALSQCSVD VYKKVGSLYP EMSAHERSLD FLIELLHKDQ LDETVNVEPL 

       730        740        750        760        770        780 
TKAIKYYQHL YSIHLAEQPE DSTMQLADHI KFTQSALDCM GVEVGRLRAF LQGGQEATDI 

       790        800        810        820        830        840 
ALLLRDLETS CSDTRQFCKK IRRRMPGTDA PGIPAALAFG SQVSDTLLDC RKHLTWVVAV 

       850        860        870        880        890        900 
LQEVAAAAAQ LIAPLAENEG LPVAALEELA FKASEQIYGS PSSSPYECLR QSCTILISTM 

       910        920        930        940        950        960 
NKLATAMQEG EYDAERPPSK PPPVELRAAA LRAEITDAEG LGLKLEDRET VIKELKKSLK 

       970        980        990       1000       1010       1020 
IKGEELSEAN VRLSLLEKKL DSAAKDADER IEKVQTRLDE TQTLLRKKEK DFEETMDALQ 

      1030       1040       1050       1060       1070       1080 
ADIDQLEAEK AELKQRLNSQ SKRTIEGLRG PPPSGIATLV SGIAGEEPQR GGAPGQAPGA 

      1090       1100       1110       1120       1130       1140 
LPGPGLVKDS PLLLQQISAM RLHISQLQHE NSILRGAQMK ASLAALPPLH VAKLSLPPHE 

      1150       1160       1170       1180       1190       1200 
GPGGNLVAGA LYRKTSQLLE KLNQLSTHTH VVDITRSSPA AKSPSAQLME QVAQLKSLSD 

      1210       1220       1230       1240       1250       1260 
TIEKLKDEVL KETVTQRPGA TVPTDFATFP SSAFLRAKEE QQDDTVYMGK VTFSCAAGLG 

      1270       1280 
QRHRLVLTQE QLHQLHSRLI S 

« Hide

Isoform 2 [UniParc].

Checksum: 17BD5B9ED7CD2474
Show »

FASTA1,243138,206

References

« Hide 'large scale' references
[1]"Mouse p150Glued (dynactin 1) cDNA sequence and evaluation as a candidate for the neuromuscular disease mutation mnd2."
Jang W., Weber J.S., Tokito M.K., Holzbaur E.L., Meisler M.H.
Biochem. Biophys. Res. Commun. 231:344-347(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: C57BL/6J.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Inner ear.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport."
Hong Z., Yang Y., Zhang C., Niu Y., Li K., Zhao X., Liu J.J.
Cell Res. 19:1334-1349(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SNX6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U60312 mRNA. Translation: AAB57773.1.
AK157867 mRNA. Translation: BAE34241.1.
AC160400 Genomic DNA. No translation available.
CCDSCCDS39532.1. [O08788-1]
PIRJC5368.
RefSeqNP_031861.2. NM_007835.2. [O08788-1]
UniGeneMm.6919.

3D structure databases

ProteinModelPortalO08788.
SMRO08788. Positions 25-98.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199072. 14 interactions.
IntActO08788. 23 interactions.
MINTMINT-1847327.

Chemistry

ChEMBLCHEMBL2176787.

PTM databases

PhosphoSiteO08788.

Proteomic databases

MaxQBO08788.
PaxDbO08788.
PRIDEO08788.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000113918; ENSMUSP00000109551; ENSMUSG00000031865. [O08788-2]
ENSMUST00000113919; ENSMUSP00000109552; ENSMUSG00000031865. [O08788-1]
GeneID13191.
KEGGmmu:13191.
UCSCuc009cmz.2. mouse. [O08788-1]

Organism-specific databases

CTD1639.
MGIMGI:107745. Dctn1.

Phylogenomic databases

eggNOGCOG5244.
GeneTreeENSGT00740000115080.
HOGENOMHOG000015352.
HOVERGENHBG004956.
InParanoidO08788.
KOK04648.
OMAPEDSTMQ.
TreeFamTF105246.

Gene expression databases

ArrayExpressO08788.
BgeeO08788.
CleanExMM_DCTN1.
GenevestigatorO08788.

Family and domain databases

Gene3D2.30.30.190. 1 hit.
InterProIPR000938. CAP-Gly_domain.
IPR027663. DCTN1.
IPR022157. Dynactin.
[Graphical view]
PANTHERPTHR18916:SF29. PTHR18916:SF29. 1 hit.
PfamPF01302. CAP_GLY. 1 hit.
PF12455. Dynactin. 1 hit.
[Graphical view]
SMARTSM01052. CAP_GLY. 1 hit.
[Graphical view]
SUPFAMSSF74924. SSF74924. 1 hit.
PROSITEPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDCTN1. mouse.
NextBio283312.
PROO08788.
SOURCESearch...

Entry information

Entry nameDCTN1_MOUSE
AccessionPrimary (citable) accession number: O08788
Secondary accession number(s): E9QLJ1, Q3TZG7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot