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Protein

Dynactin subunit 1

Gene

Dctn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule. Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon. Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitement to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole. Plays a role in primary cilia formation.By similarity

GO - Molecular functioni

  • microtubule binding Source: UniProtKB
  • motor activity Source: UniProtKB-KW
  • tubulin binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-MMU-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynactin subunit 1
Alternative name(s):
150 kDa dynein-associated polypeptide
DAP-150
Short name:
DP-150
p150-glued
Gene namesi
Name:Dctn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:107745. Dctn1.

Subcellular locationi

GO - Cellular componenti

  • cell leading edge Source: MGI
  • centriole Source: UniProtKB
  • centrosome Source: UniProtKB
  • cytosol Source: GOC
  • dynactin complex Source: InterPro
  • dynein complex Source: UniProtKB-KW
  • kinetochore Source: MGI
  • membrane Source: MGI
  • microtubule Source: MGI
  • microtubule plus-end Source: UniProtKB
  • nuclear envelope Source: UniProtKB
  • protein complex Source: MGI
  • retromer complex Source: Ensembl
  • spindle Source: UniProtKB
  • spindle pole Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176787.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000835191 – 1281Dynactin subunit 1Add BLAST1281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei108PhosphothreonineBy similarity1
Modified residuei145PhosphothreonineBy similarity1
Modified residuei146PhosphothreonineBy similarity1
Modified residuei147PhosphothreonineBy similarity1
Modified residuei179Phosphoserine; by PLK1By similarity1
Modified residuei212Phosphoserine; by CDK1By similarity1

Post-translational modificationi

Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome.By similarity
Phosphorylation by SLK at Thr-145, Thr-146 and Thr-147 targets DCTN1 to the centrosome. It is uncertain if SLK phosphorylates all three threonines or one or two of them. PLK1-mediated phosphorylation at Ser-179 is essential for its localization in the nuclear envelope and promotes its dissociation from microtubules during early mitosis and positively regulates nuclear envelope breakdown during prophase.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO08788.
PaxDbiO08788.
PeptideAtlasiO08788.
PRIDEiO08788.

PTM databases

iPTMnetiO08788.
PhosphoSitePlusiO08788.
SwissPalmiO08788.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031865.
CleanExiMM_DCTN1.
ExpressionAtlasiO08788. baseline and differential.
GenevisibleiO08788. MM.

Interactioni

Subunit structurei

Monomer and homodimer. Dynactin is a large macromolecular complex of at least 10 components; p150(glued) binds directly to microtubules and to cytoplasmic dynein. Interacts with the C-terminus of MAPRE1, MAPRE2 and MAPRE3. Interacts with FBXL5. Interacts with ECM29. Interacts with CLIP1. Interacts with CLN3 and DYNAP. Interacts with MISP; this interaction regulates its distribution at the cell cortex. Interacts with CEP131 (By similarity). Interacts with SNX6 (PubMed:19935774). Interacts with CEP126. Interacts with dynein intermediate chain and dynein heavy chain. Interacts with PLK1 (via POLO-box domain). Interacts with TBCB and PARD6A (By similarity). Binds preferentially to tyrosinated microtubules than to detyrosinated microtubules (PubMed:16954346). Interacts with KIF3A (PubMed:23386061). Interacts with HPS6 (PubMed:25189619).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cep290Q6A0782EBI-776180,EBI-1811999
Kif3aP287414EBI-776180,EBI-6169413
Kif5aP331752EBI-776180,EBI-349710

GO - Molecular functioni

  • microtubule binding Source: UniProtKB
  • tubulin binding Source: MGI

Protein-protein interaction databases

BioGridi199072. 28 interactors.
DIPiDIP-32057N.
IntActiO08788. 39 interactors.
MINTiMINT-1847327.
STRINGi10090.ENSMUSP00000109552.

Structurei

3D structure databases

ProteinModelPortaliO08788.
SMRiO08788.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 90CAP-GlyPROSITE-ProRule annotationAdd BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni911 – 1281Interaction with HPS61 PublicationAdd BLAST371

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili214 – 547Sequence analysisAdd BLAST334
Coiled coili943 – 1049Sequence analysisAdd BLAST107
Coiled coili1185 – 1214Sequence analysisAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi157 – 184Ser-richAdd BLAST28

Domaini

The CAP-Gly domain is essential for interactions with microtubules and its binding partners and for its motion along the microtubules. Essential for its preferential binding to tyrosinated microtubules and for promoting the sustained interaction of the dynein motor with microtubules.By similarity

Sequence similaritiesi

Belongs to the dynactin 150 kDa subunit family.Curated
Contains 1 CAP-Gly domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0971. Eukaryota.
COG5244. LUCA.
GeneTreeiENSGT00860000133787.
HOGENOMiHOG000015352.
HOVERGENiHBG004956.
InParanoidiO08788.
KOiK04648.
OMAiPEDSTMQ.
OrthoDBiEOG091G0WO0.
TreeFamiTF105246.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
InterProiIPR000938. CAP-Gly_domain.
IPR027663. DCTN1.
IPR022157. Dynactin.
[Graphical view]
PANTHERiPTHR18916:SF40. PTHR18916:SF40. 1 hit.
PfamiPF01302. CAP_GLY. 1 hit.
PF12455. Dynactin. 1 hit.
[Graphical view]
SMARTiSM01052. CAP_GLY. 1 hit.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 1 hit.
PROSITEiPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O08788-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQSRRHMSS RTPSGSRMST EASARPLRVG SRVEVIGKGH RGTVAYVGAT
60 70 80 90 100
LFATGKWVGV ILDEAKGKND GTVQGRKYFT CDEGHGIFVR QSQIQVFEDG
110 120 130 140 150
ADTTSPETPD SSASKVLKRE GADAAAKTSK LRGLKPKKAP TARKTTTRRP
160 170 180 190 200
KPTRPASTGV AGPSSSLGPS GSASAGELSS SEPSTPAQTP LAAPIIPTPA
210 220 230 240 250
LTSPGAAPPL PSPSKEEEGL RAQVRDLEEK LETLRLKRSE DKAKLKELEK
260 270 280 290 300
HKIQLEQVQE WKSKMQEQQA DLQRRLKEAR KEAKEALEAK ERYMEEMADT
310 320 330 340 350
ADAIEMATLD KEMAEERAES LQQEVEALKE RVDELTTDLE ILKAEIEEKG
360 370 380 390 400
SDGAASSYQL KQLEEQNARL KDALVRMRDL SSSEKQEHVK LQKLMEKKNQ
410 420 430 440 450
ELEVVRQQRE RLQEELSQAE STIDELKEQV DAALGAEEMV EMLTDRNLNL
460 470 480 490 500
EEKVRELRET VGDLEAMNEM NDELQENARE TELELREQLD MAGARVREAQ
510 520 530 540 550
KRVEAAQETV ADYQQTIKKY RQLTAHLQDV NRELTNQQEA SVERQQQPPP
560 570 580 590 600
ETFDFKIKFA ETKAHAKAIE MELRQMEVAQ ANRHMSLLTA FMPDSFLRPG
610 620 630 640 650
GDHDCVLVLL LMPRLICKAE LIRKQAQEKF DLSENCSERP GLRGAAGEQL
660 670 680 690 700
SFAAGLVYSL SLLQATLHRY EHALSQCSVD VYKKVGSLYP EMSAHERSLD
710 720 730 740 750
FLIELLHKDQ LDETVNVEPL TKAIKYYQHL YSIHLAEQPE DSTMQLADHI
760 770 780 790 800
KFTQSALDCM GVEVGRLRAF LQGGQEATDI ALLLRDLETS CSDTRQFCKK
810 820 830 840 850
IRRRMPGTDA PGIPAALAFG SQVSDTLLDC RKHLTWVVAV LQEVAAAAAQ
860 870 880 890 900
LIAPLAENEG LPVAALEELA FKASEQIYGS PSSSPYECLR QSCTILISTM
910 920 930 940 950
NKLATAMQEG EYDAERPPSK PPPVELRAAA LRAEITDAEG LGLKLEDRET
960 970 980 990 1000
VIKELKKSLK IKGEELSEAN VRLSLLEKKL DSAAKDADER IEKVQTRLDE
1010 1020 1030 1040 1050
TQTLLRKKEK DFEETMDALQ ADIDQLEAEK AELKQRLNSQ SKRTIEGLRG
1060 1070 1080 1090 1100
PPPSGIATLV SGIAGEEPQR GGAPGQAPGA LPGPGLVKDS PLLLQQISAM
1110 1120 1130 1140 1150
RLHISQLQHE NSILRGAQMK ASLAALPPLH VAKLSLPPHE GPGGNLVAGA
1160 1170 1180 1190 1200
LYRKTSQLLE KLNQLSTHTH VVDITRSSPA AKSPSAQLME QVAQLKSLSD
1210 1220 1230 1240 1250
TIEKLKDEVL KETVTQRPGA TVPTDFATFP SSAFLRAKEE QQDDTVYMGK
1260 1270 1280
VTFSCAAGLG QRHRLVLTQE QLHQLHSRLI S
Length:1,281
Mass (Da):141,676
Last modified:July 27, 2011 - v3
Checksum:iA6CEDCAAA7022EB5
GO
Isoform 2 (identifier: O08788-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1049-1086: Missing.

Note: No experimental confirmation available.
Show »
Length:1,243
Mass (Da):138,206
Checksum:i17BD5B9ED7CD2474
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti406R → W in BAE34241 (PubMed:16141072).Curated1
Sequence conflicti721 – 723TKA → NKG in AAB57773 (PubMed:9070275).Curated3
Sequence conflicti732S → R in AAB57773 (PubMed:9070275).Curated1
Sequence conflicti1202I → V in BAE34241 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0295841049 – 1086Missing in isoform 2. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60312 mRNA. Translation: AAB57773.1.
AK157867 mRNA. Translation: BAE34241.1.
AC160400 Genomic DNA. No translation available.
CCDSiCCDS39532.1. [O08788-1]
PIRiJC5368.
RefSeqiNP_031861.2. NM_007835.2. [O08788-1]
UniGeneiMm.6919.

Genome annotation databases

EnsembliENSMUST00000113918; ENSMUSP00000109551; ENSMUSG00000031865. [O08788-2]
ENSMUST00000113919; ENSMUSP00000109552; ENSMUSG00000031865. [O08788-1]
GeneIDi13191.
KEGGimmu:13191.
UCSCiuc009cmz.2. mouse. [O08788-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60312 mRNA. Translation: AAB57773.1.
AK157867 mRNA. Translation: BAE34241.1.
AC160400 Genomic DNA. No translation available.
CCDSiCCDS39532.1. [O08788-1]
PIRiJC5368.
RefSeqiNP_031861.2. NM_007835.2. [O08788-1]
UniGeneiMm.6919.

3D structure databases

ProteinModelPortaliO08788.
SMRiO08788.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199072. 28 interactors.
DIPiDIP-32057N.
IntActiO08788. 39 interactors.
MINTiMINT-1847327.
STRINGi10090.ENSMUSP00000109552.

Chemistry databases

ChEMBLiCHEMBL2176787.

PTM databases

iPTMnetiO08788.
PhosphoSitePlusiO08788.
SwissPalmiO08788.

Proteomic databases

MaxQBiO08788.
PaxDbiO08788.
PeptideAtlasiO08788.
PRIDEiO08788.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113918; ENSMUSP00000109551; ENSMUSG00000031865. [O08788-2]
ENSMUST00000113919; ENSMUSP00000109552; ENSMUSG00000031865. [O08788-1]
GeneIDi13191.
KEGGimmu:13191.
UCSCiuc009cmz.2. mouse. [O08788-1]

Organism-specific databases

CTDi1639.
MGIiMGI:107745. Dctn1.

Phylogenomic databases

eggNOGiKOG0971. Eukaryota.
COG5244. LUCA.
GeneTreeiENSGT00860000133787.
HOGENOMiHOG000015352.
HOVERGENiHBG004956.
InParanoidiO08788.
KOiK04648.
OMAiPEDSTMQ.
OrthoDBiEOG091G0WO0.
TreeFamiTF105246.

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-MMU-8854518. AURKA Activation by TPX2.

Miscellaneous databases

ChiTaRSiDctn1. mouse.
PROiO08788.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031865.
CleanExiMM_DCTN1.
ExpressionAtlasiO08788. baseline and differential.
GenevisibleiO08788. MM.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
InterProiIPR000938. CAP-Gly_domain.
IPR027663. DCTN1.
IPR022157. Dynactin.
[Graphical view]
PANTHERiPTHR18916:SF40. PTHR18916:SF40. 1 hit.
PfamiPF01302. CAP_GLY. 1 hit.
PF12455. Dynactin. 1 hit.
[Graphical view]
SMARTiSM01052. CAP_GLY. 1 hit.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 1 hit.
PROSITEiPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCTN1_MOUSE
AccessioniPrimary (citable) accession number: O08788
Secondary accession number(s): E9QLJ1, Q3TZG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.