O08786 (CCKAR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cholecystokinin receptor type A
Short name=CCK-A receptor
Short name=CCK-AR

Alternative name(s):
Cholecystokinin-1 receptor
Short name=CCK1-R

Gene names

Name:

Cckar

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	axonogenesis Inferred from mutant phenotype. Source: MGI forebrain development Inferred from mutant phenotype. Source: MGI neuron migration Inferred from mutant phenotype. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholecystokinin receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Cholecystokinin receptor type A		PRO_0000069224
Regions
	Topological domain	1 – 41	41	Extracellular Potential
	Transmembrane	42 – 67	26	Helical; Name=1; Potential
	Topological domain	68 – 77	10	Cytoplasmic Potential
	Transmembrane	78 – 104	27	Helical; Name=2; Potential
	Topological domain	105 – 115	11	Extracellular Potential
	Transmembrane	116 – 137	22	Helical; Name=3; Potential
	Topological domain	138 – 157	20	Cytoplasmic Potential
	Transmembrane	158 – 178	21	Helical; Name=4; Potential
	Topological domain	179 – 210	32	Extracellular Potential
	Transmembrane	211 – 234	24	Helical; Name=5; Potential
	Topological domain	235 – 321	87	Cytoplasmic Potential
	Transmembrane	322 – 342	21	Helical; Name=6; Potential
	Topological domain	343 – 357	15	Extracellular Potential
	Transmembrane	358 – 381	24	Helical; Name=7; Potential
	Topological domain	382 – 436	55	Cytoplasmic Potential
	Compositional bias	258 – 264	7	Poly-Gly
	Compositional bias	265 – 268	4	Poly-Ser
Amino acid modifications
	Lipidation	395	1	S-palmitoyl cysteine By similarity
	Glycosylation	10	1	N-linked (GlcNAc...) Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	190	1	N-linked (GlcNAc...) Potential
	Disulfide bond	18 ↔ 29		By similarity
	Disulfide bond	114 ↔ 196		By similarity
Experimental info
	Sequence conflict	34	1	Q → H in BAB23512. Ref.3
	Sequence conflict	59	1	N → D in BAB23512. Ref.3
	Sequence conflict	436	1	H → P in AAH20534. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O08786 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: EBB5D8453B792F79	FASTA	436	48,437
	10 20 30 40 50 60 MDVVDSLLMN GSNITPPCEL GLENETLFCL DQPQPSKEWQ SAVQILLYSF IFLLSVLGNT 70 80 90 100 110 120 LVITVLIRNK RMRTVTNIFL LSLAVSDLML CLFCMPFNLI PNLLKDFIFG SAVCKTTTYF 130 140 150 160 170 180 MGTSVSVSTF NLVAISLERY GAICRPLQSR VWQTKSHALK VIAATWCLSF TIMTPYPIYS 190 200 210 220 230 240 NLVPFTKNNN QTANMCRFLL PSDAMQQSWQ TFLLLILFLI PGVVMVVAYG LISLELYQGI 250 260 270 280 290 300 KFDASQKKSA KEKRLSSGGG GGGGSSSSRY EDSDGCYLQK SRPPRKLELQ QLSTSSSGGR 310 320 330 340 350 360 INRIRSSGSA ANLIAKKRVI RMLIVIVVLF FLCWMPIFSA NAWRAYDTVS AEKHLSGTPI 370 380 390 400 410 420 SFILLLSYTS SCVNPIIYCF MNKRFRLGFM ATFPCCPNPG PTGVRGEVGE EEDGRTIRAS 430 LSRYSYSHMS TSAPPH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse cholecystokinin type-A receptor gene and its structural analysis." Takata Y., Takiguchi S., Takaoka K., Funakoshi A., Miyasaka K., Kono A. Gene 187:267-271(1997) [PubMed: 9099891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ. Tissue: Liver.
[2]	"Molecular structure of the mouse CCK-A receptor gene." Lacourse K.A., Lay J.M., Swanberg L.J., Jenkins C., Samuelson L.C. Biochem. Biophys. Res. Commun. 236:630-635(1997) [PubMed: 9245702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 129/SvJ.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF015963 , AF015959, AF015960, AF015961, AF015962 Genomic DNA. Translation: AAC07949.1. D85605 Genomic DNA. Translation: BAA20068.1. AK004730 mRNA. Translation: BAB23512.1. BC020534 mRNA. Translation: AAH20534.1.
IPI	IPI00115662.
PIR	JC5599.
RefSeq	NP_033957.1. NM_009827.2.
UniGene	Mm.3521.
3D structure databases
ProteinModelPortal	O08786.
SMR	O08786. Positions 1-410.
ModBase	Search...
Protein-protein interaction databases
STRING	O08786.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	O08786.
Proteomic databases
PRIDE	O08786.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000031093; ENSMUSP00000031093; ENSMUSG00000029193.
GeneID	12425.
KEGG	mmu:12425.
UCSC	uc008xlm.2. mouse.
Organism-specific databases
CTD	886.
MGI	MGI:99478. Cckar.
Phylogenomic databases
eggNOG	roNOG15027.
HOGENOM	HBG445005.
HOVERGEN	HBG036927.
InParanoid	O08786.
OMA	TTTYFMG.
OrthoDB	EOG43N7D1.
PhylomeDB	O08786.
Gene expression databases
ArrayExpress	O08786.
Bgee	O08786.
CleanEx	MM_CCKAR.
Genevestigator	O08786.
GermOnline	ENSMUSG00000029193. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR009126. Cholcskin_rcpt. IPR000596. Cholcy_rcpt_A. IPR015276. CholecystokininA_recpt_N. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
Gene3D	G3DSA:4.10.670.10. CholecystokininA_recpt_N. 1 hit.
KO	K04194.
Pfam	PF00001. 7tm_1. 1 hit. PF09193. CholecysA-Rec_N. 1 hit. [Graphical view]
PRINTS	PR01822. CCYSTOKININR. PR00524. CCYSTOKNINAR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281232.
SOURCE	Search...

Entry information

Entry name

CCKAR_MOUSE

Accession

Primary (citable) accession number: O08786
Secondary accession number(s): Q8VCC7, Q9DBV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 1, 1997
Last modified:	November 16, 2011
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program