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Protein

Treacle protein

Gene

Tcof1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in nucleolar-cytoplasmic transport. May play a fundamental role in early embryonic development, particularly in development of the craniofacial complex. May participate in certain stages of ribosome biogenesis.

GO - Molecular functioni

GO - Biological processi

  • transcription of nuclear large rRNA transcript from RNA polymerase I promoter Source: MGI
  • transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Treacle protein
Alternative name(s):
Treacher Collins syndrome protein homolog
Gene namesi
Name:Tcof1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:892003. Tcof1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000724601 – 1320Treacle proteinAdd BLAST1320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei83PhosphoserineBy similarity1
Modified residuei85PhosphoserineBy similarity1
Modified residuei87PhosphoserineBy similarity1
Modified residuei88PhosphoserineBy similarity1
Modified residuei104PhosphothreonineCombined sources1
Cross-linki126Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei132N6-acetyllysineCombined sources1
Modified residuei151PhosphoserineBy similarity1
Modified residuei153N6-acetyllysineBy similarity1
Modified residuei154PhosphoserineCombined sources1
Modified residuei169PhosphoserineCombined sources1
Modified residuei171PhosphothreonineCombined sources1
Modified residuei301N6-acetyllysineBy similarity1
Modified residuei318N6-acetyllysineCombined sources1
Modified residuei324PhosphothreonineCombined sources1
Modified residuei327N6-acetyllysineCombined sources1
Modified residuei386PhosphoserineBy similarity1
Modified residuei413PhosphoserineCombined sources1
Modified residuei414PhosphoserineCombined sources1
Modified residuei433PhosphoserineBy similarity1
Modified residuei443PhosphoserineBy similarity1
Modified residuei521N6-acetyllysine; alternateBy similarity1
Cross-linki521Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei590PhosphoserineCombined sources1
Modified residuei593PhosphoserineCombined sources1
Modified residuei626N6-acetyllysineCombined sources1
Modified residuei630N6-acetyllysineCombined sources1
Modified residuei655N6-acetyllysine; alternateBy similarity1
Cross-linki655Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki655Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei662PhosphoserineBy similarity1
Modified residuei664PhosphoserineBy similarity1
Modified residuei669PhosphoserineBy similarity1
Modified residuei671PhosphoserineBy similarity1
Modified residuei760PhosphoserineBy similarity1
Modified residuei761PhosphoserineBy similarity1
Modified residuei765PhosphoserineBy similarity1
Modified residuei767PhosphoserineBy similarity1
Modified residuei794PhosphoserineBy similarity1
Modified residuei853PhosphoserineBy similarity1
Modified residuei860PhosphothreonineCombined sources1
Modified residuei869PhosphothreonineBy similarity1
Modified residuei922N6-acetyllysineCombined sources1
Modified residuei976N6-acetyllysineCombined sources1
Modified residuei1034PhosphoserineBy similarity1
Modified residuei1066PhosphothreonineCombined sources1
Modified residuei1191PhosphoserineCombined sources1
Modified residuei1216PhosphoserineCombined sources1
Modified residuei1242PhosphoserineBy similarity1
Modified residuei1249N6-acetyllysine; alternateBy similarity1
Cross-linki1249Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei1301PhosphoserineCombined sources1
Modified residuei1303PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO08784.
MaxQBiO08784.
PaxDbiO08784.
PeptideAtlasiO08784.
PRIDEiO08784.

PTM databases

iPTMnetiO08784.
PhosphoSitePlusiO08784.

Miscellaneous databases

PMAP-CutDBO08784.

Expressioni

Tissue specificityi

Ubiquitous in adult and embryonic tissues.

Developmental stagei

Expression elevated at 11 dpc when the branchial arches and facial swellings are present.1 Publication

Gene expression databases

BgeeiENSMUSG00000024613.
ExpressionAtlasiO08784. baseline and differential.
GenevisibleiO08784. MM.

Interactioni

Subunit structurei

Part of a large pre-ribosomal ribonucleoprotein (RNP) complex, that consists of at least 62 ribosomal proteins, 45 nonribosomal proteins and both pre-rRNA and mature rRNA species. Within this complex directly interacts with NOP56 in an RNA-independent manner (By similarity).By similarity

Protein-protein interaction databases

BioGridi204043. 1 interactor.
IntActiO08784. 2 interactors.
MINTiMINT-4137233.
STRINGi10090.ENSMUSP00000135639.

Structurei

3D structure databases

ProteinModelPortaliO08784.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 38LisHPROSITE-ProRule annotationAdd BLAST33
Repeati212 – 2951Add BLAST84
Repeati296 – 3662Add BLAST71
Repeati367 – 4363Add BLAST70
Repeati437 – 5054Add BLAST69
Repeati506 – 5505Add BLAST45
Repeati551 – 6246Add BLAST74
Repeati625 – 6807Add BLAST56
Repeati681 – 7148Add BLAST34
Repeati715 – 7749Add BLAST60
Repeati775 – 83910Add BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 83910 X approximate tandem repeatsAdd BLAST628

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi195 – 198Poly-Ser4
Compositional biasi216 – 272Ala-richAdd BLAST57
Compositional biasi253 – 272Poly-AlaAdd BLAST20
Compositional biasi880 – 885Poly-Ser6
Compositional biasi1179 – 1314Lys-richAdd BLAST136
Compositional biasi1200 – 1205Poly-Lys6
Compositional biasi1270 – 1277Poly-Lys8
Compositional biasi1280 – 1288Poly-Lys9
Compositional biasi1307 – 1314Poly-Lys8

Sequence similaritiesi

Contains 1 LisH domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IF55. Eukaryota.
ENOG411183W. LUCA.
GeneTreeiENSGT00730000111382.
HOGENOMiHOG000154524.
HOVERGENiHBG006664.
InParanoidiO08784.
KOiK14562.
PhylomeDBiO08784.
TreeFamiTF341730.

Family and domain databases

InterProiIPR006594. LisH.
IPR003993. TCS_treacle.
IPR017859. Treacle-like_TCS.
[Graphical view]
PfamiPF03546. Treacle. 2 hits.
[Graphical view]
PRINTSiPR01503. TREACLE.
SMARTiSM00667. LisH. 1 hit.
[Graphical view]
PROSITEiPS50896. LISH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08784-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEARKRREL LPLIYHHLLQ AGYVRAAREV KEQSGQKSFL TQPVTLLDIY
60 70 80 90 100
THWQQTSELG QKQKAEDDET LQAKKSRVSD PVSSSESSDQ EKEEEAATER
110 120 130 140 150
AKATPRPTPV NSATAALPSK VKEKGKTKTA NKTVNSVSHP GSGKTVVHLL
160 170 180 190 200
SGKSPKKSAE PLANTVLASE TEEEGNAQAL GPTAKSGTVS AGQGSSSSED
210 220 230 240 250
SSISSDETDV EVKSPAKPAQ AKASAAPAKD PPARTAPGPT KLGNVAPTPA
260 270 280 290 300
KPARAAAAAA AAAVAAAAAA AAEESESSEE DSDSEDEAPA GLPSQVKASG
310 320 330 340 350
KGPHVRADSV SAKGISGKGP ILATPGKTGP AATQAKAERP EKDSETSSED
360 370 380 390 400
DSDSEDEMPV TVNTPQARTS GKSPRARGTS APAKESSQKG APAVTPGKAR
410 420 430 440 450
PVAAQAGKPE AKSSEESESD SGETPAAATL TTSPAKVKPL GKSSQVRPVS
460 470 480 490 500
TVTPGSSGKG ANLPCPGKVG SAALRVQMVK KEDVSESSSA ELDSDGPGSP
510 520 530 540 550
AKAKASLALP QKVRPVATQV KTDRGKGHSG SSEESSDSEE EAAPAASAAQ
560 570 580 590 600
AKPALEKQMK ASSRKGTPAS ATGASTSSHC KAGAVTSSAS LSSPALAKGT
610 620 630 640 650
QRSDVDSSSE SESEGAAPST PRVQGKSGGK GLQGKAALGQ GVAPVHTQKT
660 670 680 690 700
GPSVKAMAQE DSESLEEDSS SEEEDETPAQ ATPLGRLPQA KANPPPTKTP
710 720 730 740 750
PASASGKAVA APTKGKPPVP NSTVSARGQR SVPAAGKAGA PATQAQKGPV
760 770 780 790 800
AGTGEDSESS SKEESDSEEE TPAQIKPVGK TSQVRAASAP AKESPKKGAH
810 820 830 840 850
PGTPGKTGSS ATQAQPGKTE DSDSSSEESD SDTEMPSAQA IKSPPVSVNR
860 870 880 890 900
NSSPAVPAPT PEGVQAVNTT KKASGTTAQS SSSESEDGDE DLIPATQPST
910 920 930 940 950
YALRTSVTTP AALSRAASQP SKSEQSSRMP KGKKAKAAAS AQTSSAVETL
960 970 980 990 1000
PMMPPQSAPI QPKATNKLGK SKLPEKQQLA PGYPKAPRSS EDSSDTSSED
1010 1020 1030 1040 1050
EEDAKRPQMP KSAHRLDPDP SQKETVVEET PTESSEDEMV APSQSLLSGY
1060 1070 1080 1090 1100
MTPGLTVANS QASKATPRPD SNSLASSAPA TKDNPDGKQK SKSQHAADTA
1110 1120 1130 1140 1150
LPKTGRKEAS SGSTPQKPKK LKKSTSSSPA PTQTLPNSIT QRLLEQAWPL
1160 1170 1180 1190 1200
SEAQVQASVV KVLTELLEQE RLKATEAIKE SGKKSQKRKL SGDLEAGAPK
1210 1220 1230 1240 1250
NKKKKEQPVP RASAVSPEKA PMTSKAKSKL DKGSAGGKGK GSPGPQGAKE
1260 1270 1280 1290 1300
KPDGELLGIK LESGEQSDPK SKSKKKKSLK KKKDKEKKEK KKGKKSLAKD
1310 1320
SASPIQKKKK KKKKSAEPAV
Length:1,320
Mass (Da):135,001
Last modified:July 1, 1997 - v1
Checksum:i34D87F5F5D300758
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35G → A in AAB60933 (PubMed:9158147).Curated1
Sequence conflicti125 – 142Missing in AAB60933 (PubMed:9158147).CuratedAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001794 mRNA. Translation: AAB71347.1.
U81030 mRNA. Translation: AAB60933.1.
BC060105 mRNA. Translation: AAH60105.1.
CCDSiCCDS37836.1.
PIRiJC5630.
RefSeqiNP_035682.1. NM_011552.3.
UniGeneiMm.486251.

Genome annotation databases

EnsembliENSMUST00000176630; ENSMUSP00000135476; ENSMUSG00000024613.
GeneIDi21453.
KEGGimmu:21453.
UCSCiuc008fbb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001794 mRNA. Translation: AAB71347.1.
U81030 mRNA. Translation: AAB60933.1.
BC060105 mRNA. Translation: AAH60105.1.
CCDSiCCDS37836.1.
PIRiJC5630.
RefSeqiNP_035682.1. NM_011552.3.
UniGeneiMm.486251.

3D structure databases

ProteinModelPortaliO08784.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204043. 1 interactor.
IntActiO08784. 2 interactors.
MINTiMINT-4137233.
STRINGi10090.ENSMUSP00000135639.

PTM databases

iPTMnetiO08784.
PhosphoSitePlusiO08784.

Proteomic databases

EPDiO08784.
MaxQBiO08784.
PaxDbiO08784.
PeptideAtlasiO08784.
PRIDEiO08784.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000176630; ENSMUSP00000135476; ENSMUSG00000024613.
GeneIDi21453.
KEGGimmu:21453.
UCSCiuc008fbb.2. mouse.

Organism-specific databases

CTDi6949.
MGIiMGI:892003. Tcof1.

Phylogenomic databases

eggNOGiENOG410IF55. Eukaryota.
ENOG411183W. LUCA.
GeneTreeiENSGT00730000111382.
HOGENOMiHOG000154524.
HOVERGENiHBG006664.
InParanoidiO08784.
KOiK14562.
PhylomeDBiO08784.
TreeFamiTF341730.

Miscellaneous databases

PMAP-CutDBO08784.
PROiO08784.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024613.
ExpressionAtlasiO08784. baseline and differential.
GenevisibleiO08784. MM.

Family and domain databases

InterProiIPR006594. LisH.
IPR003993. TCS_treacle.
IPR017859. Treacle-like_TCS.
[Graphical view]
PfamiPF03546. Treacle. 2 hits.
[Graphical view]
PRINTSiPR01503. TREACLE.
SMARTiSM00667. LisH. 1 hit.
[Graphical view]
PROSITEiPS50896. LISH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTCOF_MOUSE
AccessioniPrimary (citable) accession number: O08784
Secondary accession number(s): O08857
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.