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O08784 (TCOF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Treacle protein
Alternative name(s):
Treacher Collins syndrome protein homolog
Gene names
Name:Tcof1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1320 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in nucleolar-cytoplasmic transport. May play a fundamental role in early embryonic development, particularly in development of the craniofacial complex. May participate in certain stages of ribosome biogenesis.

Subunit structure

Part of a large pre-ribosomal ribonucleoprotein (RNP) complex, that consists of at least 62 ribosomal proteins, 45 nonribosomal proteins and both pre-rRNA and mature rRNA species. Within this complex directly interacts with NOP56 in an RNA-independent manner By similarity.

Subcellular location

Nucleusnucleolus By similarity.

Tissue specificity

Ubiquitous in adult and embryonic tissues.

Developmental stage

Expression elevated at 11 dpc when the branchial arches and facial swellings are present. Ref.2

Sequence similarities

Contains 1 LisH domain.

Ontologies

Keywords
   Biological processTransport
   Cellular componentNucleus
   DomainRepeat
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranscription of nuclear large rRNA transcript from RNA polymerase I promoter

Inferred from mutant phenotype PubMed 15249688. Source: MGI

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleolus

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13201320Treacle protein
PRO_0000072460

Regions

Domain6 – 3833LisH
Repeat212 – 295841
Repeat296 – 366712
Repeat367 – 436703
Repeat437 – 505694
Repeat506 – 550455
Repeat551 – 624746
Repeat625 – 680567
Repeat681 – 714348
Repeat715 – 774609
Repeat775 – 8396510
Region212 – 83962810 X approximate tandem repeats
Compositional bias195 – 1984Poly-Ser
Compositional bias216 – 27257Ala-rich
Compositional bias253 – 27220Poly-Ala
Compositional bias880 – 8856Poly-Ser
Compositional bias1179 – 1314136Lys-rich
Compositional bias1200 – 12056Poly-Lys
Compositional bias1270 – 12778Poly-Lys
Compositional bias1280 – 12889Poly-Lys
Compositional bias1307 – 13148Poly-Lys

Amino acid modifications

Modified residue831Phosphoserine By similarity
Modified residue851Phosphoserine By similarity
Modified residue871Phosphoserine By similarity
Modified residue881Phosphoserine By similarity
Modified residue1321N6-acetyllysine Ref.8
Modified residue1511Phosphoserine By similarity
Modified residue1531N6-acetyllysine By similarity
Modified residue1541Phosphoserine By similarity
Modified residue1691Phosphoserine Ref.5 Ref.7
Modified residue1711Phosphothreonine Ref.5 Ref.7
Modified residue3011N6-acetyllysine By similarity
Modified residue3181N6-acetyllysine Ref.8
Modified residue3271N6-acetyllysine Ref.8
Modified residue3861Phosphoserine By similarity
Modified residue4331Phosphoserine By similarity
Modified residue4431Phosphoserine By similarity
Modified residue5211N6-acetyllysine By similarity
Modified residue5931Phosphoserine Ref.7
Modified residue6261N6-acetyllysine Ref.8
Modified residue6301N6-acetyllysine Ref.8
Modified residue6551N6-acetyllysine By similarity
Modified residue6621Phosphoserine By similarity
Modified residue6641Phosphoserine By similarity
Modified residue6691Phosphoserine By similarity
Modified residue6711Phosphoserine By similarity
Modified residue7601Phosphoserine By similarity
Modified residue7611Phosphoserine By similarity
Modified residue7651Phosphoserine By similarity
Modified residue7671Phosphoserine By similarity
Modified residue7941Phosphoserine By similarity
Modified residue8531Phosphoserine By similarity
Modified residue8691Phosphothreonine By similarity
Modified residue9221N6-acetyllysine Ref.8
Modified residue9761N6-acetyllysine Ref.8
Modified residue10341Phosphoserine By similarity
Modified residue11911Phosphoserine Ref.6
Modified residue12161Phosphoserine Ref.7
Modified residue12421Phosphoserine By similarity
Modified residue12491N6-acetyllysine By similarity

Experimental info

Sequence conflict351G → A in AAB60933. Ref.2
Sequence conflict125 – 14218Missing in AAB60933. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O08784 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 34D87F5F5D300758

FASTA1,320135,001
        10         20         30         40         50         60 
MAEARKRREL LPLIYHHLLQ AGYVRAAREV KEQSGQKSFL TQPVTLLDIY THWQQTSELG 

        70         80         90        100        110        120 
QKQKAEDDET LQAKKSRVSD PVSSSESSDQ EKEEEAATER AKATPRPTPV NSATAALPSK 

       130        140        150        160        170        180 
VKEKGKTKTA NKTVNSVSHP GSGKTVVHLL SGKSPKKSAE PLANTVLASE TEEEGNAQAL 

       190        200        210        220        230        240 
GPTAKSGTVS AGQGSSSSED SSISSDETDV EVKSPAKPAQ AKASAAPAKD PPARTAPGPT 

       250        260        270        280        290        300 
KLGNVAPTPA KPARAAAAAA AAAVAAAAAA AAEESESSEE DSDSEDEAPA GLPSQVKASG 

       310        320        330        340        350        360 
KGPHVRADSV SAKGISGKGP ILATPGKTGP AATQAKAERP EKDSETSSED DSDSEDEMPV 

       370        380        390        400        410        420 
TVNTPQARTS GKSPRARGTS APAKESSQKG APAVTPGKAR PVAAQAGKPE AKSSEESESD 

       430        440        450        460        470        480 
SGETPAAATL TTSPAKVKPL GKSSQVRPVS TVTPGSSGKG ANLPCPGKVG SAALRVQMVK 

       490        500        510        520        530        540 
KEDVSESSSA ELDSDGPGSP AKAKASLALP QKVRPVATQV KTDRGKGHSG SSEESSDSEE 

       550        560        570        580        590        600 
EAAPAASAAQ AKPALEKQMK ASSRKGTPAS ATGASTSSHC KAGAVTSSAS LSSPALAKGT 

       610        620        630        640        650        660 
QRSDVDSSSE SESEGAAPST PRVQGKSGGK GLQGKAALGQ GVAPVHTQKT GPSVKAMAQE 

       670        680        690        700        710        720 
DSESLEEDSS SEEEDETPAQ ATPLGRLPQA KANPPPTKTP PASASGKAVA APTKGKPPVP 

       730        740        750        760        770        780 
NSTVSARGQR SVPAAGKAGA PATQAQKGPV AGTGEDSESS SKEESDSEEE TPAQIKPVGK 

       790        800        810        820        830        840 
TSQVRAASAP AKESPKKGAH PGTPGKTGSS ATQAQPGKTE DSDSSSEESD SDTEMPSAQA 

       850        860        870        880        890        900 
IKSPPVSVNR NSSPAVPAPT PEGVQAVNTT KKASGTTAQS SSSESEDGDE DLIPATQPST 

       910        920        930        940        950        960 
YALRTSVTTP AALSRAASQP SKSEQSSRMP KGKKAKAAAS AQTSSAVETL PMMPPQSAPI 

       970        980        990       1000       1010       1020 
QPKATNKLGK SKLPEKQQLA PGYPKAPRSS EDSSDTSSED EEDAKRPQMP KSAHRLDPDP 

      1030       1040       1050       1060       1070       1080 
SQKETVVEET PTESSEDEMV APSQSLLSGY MTPGLTVANS QASKATPRPD SNSLASSAPA 

      1090       1100       1110       1120       1130       1140 
TKDNPDGKQK SKSQHAADTA LPKTGRKEAS SGSTPQKPKK LKKSTSSSPA PTQTLPNSIT 

      1150       1160       1170       1180       1190       1200 
QRLLEQAWPL SEAQVQASVV KVLTELLEQE RLKATEAIKE SGKKSQKRKL SGDLEAGAPK 

      1210       1220       1230       1240       1250       1260 
NKKKKEQPVP RASAVSPEKA PMTSKAKSKL DKGSAGGKGK GSPGPQGAKE KPDGELLGIK 

      1270       1280       1290       1300       1310       1320 
LESGEQSDPK SKSKKKKSLK KKKDKEKKEK KKGKKSLAKD SASPIQKKKK KKKKSAEPAV 

« Hide

References

« Hide 'large scale' references
[1]"Mouse TCOF1 is expressed widely, has motifs conserved in nucleolar phosphoproteins, and maps to chromosome 18."
Paznekas W.A., Zhang N., Gridley T., Jabs E.W.
Biochem. Biophys. Res. Commun. 238:1-6(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Sequence analysis, identification of evolutionary conserved motifs and expression analysis of murine tcof1 provide further evidence for a potential function for the gene and its human homologue, TCOF1."
Dixon J., Hovanes K., Shiang R., Dixon M.J.
Hum. Mol. Genet. 6:727-737(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1314.
Strain: C57BL/6.
Tissue: Brain.
[4]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169 AND THR-171, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169; THR-171; SER-593 AND SER-1216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[8]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-132; LYS-318; LYS-327; LYS-626; LYS-630; LYS-922 AND LYS-976, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF001794 mRNA. Translation: AAB71347.1.
U81030 mRNA. Translation: AAB60933.1.
BC060105 mRNA. Translation: AAH60105.1.
CCDSCCDS37836.1.
PIRJC5630.
RefSeqNP_035682.1. NM_011552.3.
UniGeneMm.486251.

3D structure databases

ProteinModelPortalO08784.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204043. 1 interaction.
IntActO08784. 2 interactions.
MINTMINT-4137233.

PTM databases

PhosphoSiteO08784.

Proteomic databases

MaxQBO08784.
PaxDbO08784.
PRIDEO08784.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000176630; ENSMUSP00000135476; ENSMUSG00000024613.
GeneID21453.
KEGGmmu:21453.
UCSCuc008fbb.2. mouse.

Organism-specific databases

CTD6949.
MGIMGI:892003. Tcof1.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00730000111382.
HOGENOMHOG000154524.
HOVERGENHBG006664.
InParanoidO08784.
KOK14562.
PhylomeDBO08784.
TreeFamTF341730.

Gene expression databases

ArrayExpressO08784.
BgeeO08784.
GenevestigatorO08784.

Family and domain databases

InterProIPR006594. LisH_dimerisation.
IPR003993. TCS_treacle.
IPR017859. Treacle-like_TCS.
[Graphical view]
PfamPF03546. Treacle. 2 hits.
[Graphical view]
PRINTSPR01503. TREACLE.
SMARTSM00667. LisH. 1 hit.
[Graphical view]
PROSITEPS50896. LISH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio300816.
PMAP-CutDBO08784.
PROO08784.
SOURCESearch...

Entry information

Entry nameTCOF_MOUSE
AccessionPrimary (citable) accession number: O08784
Secondary accession number(s): O08857
Entry history
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot