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O08784

- TCOF_MOUSE

UniProt

O08784 - TCOF_MOUSE

Protein

Treacle protein

Gene

Tcof1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    May be involved in nucleolar-cytoplasmic transport. May play a fundamental role in early embryonic development, particularly in development of the craniofacial complex. May participate in certain stages of ribosome biogenesis.

    GO - Biological processi

    1. transcription of nuclear large rRNA transcript from RNA polymerase I promoter Source: MGI
    2. transport Source: UniProtKB-KW

    Keywords - Biological processi

    Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Treacle protein
    Alternative name(s):
    Treacher Collins syndrome protein homolog
    Gene namesi
    Name:Tcof1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:892003. Tcof1.

    Subcellular locationi

    Nucleusnucleolus By similarity

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13201320Treacle proteinPRO_0000072460Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei83 – 831PhosphoserineBy similarity
    Modified residuei85 – 851PhosphoserineBy similarity
    Modified residuei87 – 871PhosphoserineBy similarity
    Modified residuei88 – 881PhosphoserineBy similarity
    Modified residuei132 – 1321N6-acetyllysine1 Publication
    Modified residuei151 – 1511PhosphoserineBy similarity
    Modified residuei153 – 1531N6-acetyllysineBy similarity
    Modified residuei154 – 1541PhosphoserineBy similarity
    Modified residuei169 – 1691Phosphoserine2 Publications
    Modified residuei171 – 1711Phosphothreonine2 Publications
    Modified residuei301 – 3011N6-acetyllysineBy similarity
    Modified residuei318 – 3181N6-acetyllysine1 Publication
    Modified residuei327 – 3271N6-acetyllysine1 Publication
    Modified residuei386 – 3861PhosphoserineBy similarity
    Modified residuei433 – 4331PhosphoserineBy similarity
    Modified residuei443 – 4431PhosphoserineBy similarity
    Modified residuei521 – 5211N6-acetyllysineBy similarity
    Modified residuei593 – 5931Phosphoserine1 Publication
    Modified residuei626 – 6261N6-acetyllysine1 Publication
    Modified residuei630 – 6301N6-acetyllysine1 Publication
    Modified residuei655 – 6551N6-acetyllysineBy similarity
    Modified residuei662 – 6621PhosphoserineBy similarity
    Modified residuei664 – 6641PhosphoserineBy similarity
    Modified residuei669 – 6691PhosphoserineBy similarity
    Modified residuei671 – 6711PhosphoserineBy similarity
    Modified residuei760 – 7601PhosphoserineBy similarity
    Modified residuei761 – 7611PhosphoserineBy similarity
    Modified residuei765 – 7651PhosphoserineBy similarity
    Modified residuei767 – 7671PhosphoserineBy similarity
    Modified residuei794 – 7941PhosphoserineBy similarity
    Modified residuei853 – 8531PhosphoserineBy similarity
    Modified residuei869 – 8691PhosphothreonineBy similarity
    Modified residuei922 – 9221N6-acetyllysine1 Publication
    Modified residuei976 – 9761N6-acetyllysine1 Publication
    Modified residuei1034 – 10341PhosphoserineBy similarity
    Modified residuei1191 – 11911Phosphoserine1 Publication
    Modified residuei1216 – 12161Phosphoserine1 Publication
    Modified residuei1242 – 12421PhosphoserineBy similarity
    Modified residuei1249 – 12491N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO08784.
    PaxDbiO08784.
    PRIDEiO08784.

    PTM databases

    PhosphoSiteiO08784.

    Miscellaneous databases

    PMAP-CutDBO08784.

    Expressioni

    Tissue specificityi

    Ubiquitous in adult and embryonic tissues.

    Developmental stagei

    Expression elevated at 11 dpc when the branchial arches and facial swellings are present.1 Publication

    Gene expression databases

    ArrayExpressiO08784.
    BgeeiO08784.
    GenevestigatoriO08784.

    Interactioni

    Subunit structurei

    Part of a large pre-ribosomal ribonucleoprotein (RNP) complex, that consists of at least 62 ribosomal proteins, 45 nonribosomal proteins and both pre-rRNA and mature rRNA species. Within this complex directly interacts with NOP56 in an RNA-independent manner By similarity.By similarity

    Protein-protein interaction databases

    BioGridi204043. 1 interaction.
    IntActiO08784. 2 interactions.
    MINTiMINT-4137233.

    Structurei

    3D structure databases

    ProteinModelPortaliO08784.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini6 – 3833LisHPROSITE-ProRule annotationAdd
    BLAST
    Repeati212 – 295841Add
    BLAST
    Repeati296 – 366712Add
    BLAST
    Repeati367 – 436703Add
    BLAST
    Repeati437 – 505694Add
    BLAST
    Repeati506 – 550455Add
    BLAST
    Repeati551 – 624746Add
    BLAST
    Repeati625 – 680567Add
    BLAST
    Repeati681 – 714348Add
    BLAST
    Repeati715 – 774609Add
    BLAST
    Repeati775 – 8396510Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni212 – 83962810 X approximate tandem repeatsAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi195 – 1984Poly-Ser
    Compositional biasi216 – 27257Ala-richAdd
    BLAST
    Compositional biasi253 – 27220Poly-AlaAdd
    BLAST
    Compositional biasi880 – 8856Poly-Ser
    Compositional biasi1179 – 1314136Lys-richAdd
    BLAST
    Compositional biasi1200 – 12056Poly-Lys
    Compositional biasi1270 – 12778Poly-Lys
    Compositional biasi1280 – 12889Poly-Lys
    Compositional biasi1307 – 13148Poly-Lys

    Sequence similaritiesi

    Contains 1 LisH domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00730000111382.
    HOGENOMiHOG000154524.
    HOVERGENiHBG006664.
    InParanoidiO08784.
    KOiK14562.
    PhylomeDBiO08784.
    TreeFamiTF341730.

    Family and domain databases

    InterProiIPR006594. LisH_dimerisation.
    IPR003993. TCS_treacle.
    IPR017859. Treacle-like_TCS.
    [Graphical view]
    PfamiPF03546. Treacle. 2 hits.
    [Graphical view]
    PRINTSiPR01503. TREACLE.
    SMARTiSM00667. LisH. 1 hit.
    [Graphical view]
    PROSITEiPS50896. LISH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O08784-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEARKRREL LPLIYHHLLQ AGYVRAAREV KEQSGQKSFL TQPVTLLDIY     50
    THWQQTSELG QKQKAEDDET LQAKKSRVSD PVSSSESSDQ EKEEEAATER 100
    AKATPRPTPV NSATAALPSK VKEKGKTKTA NKTVNSVSHP GSGKTVVHLL 150
    SGKSPKKSAE PLANTVLASE TEEEGNAQAL GPTAKSGTVS AGQGSSSSED 200
    SSISSDETDV EVKSPAKPAQ AKASAAPAKD PPARTAPGPT KLGNVAPTPA 250
    KPARAAAAAA AAAVAAAAAA AAEESESSEE DSDSEDEAPA GLPSQVKASG 300
    KGPHVRADSV SAKGISGKGP ILATPGKTGP AATQAKAERP EKDSETSSED 350
    DSDSEDEMPV TVNTPQARTS GKSPRARGTS APAKESSQKG APAVTPGKAR 400
    PVAAQAGKPE AKSSEESESD SGETPAAATL TTSPAKVKPL GKSSQVRPVS 450
    TVTPGSSGKG ANLPCPGKVG SAALRVQMVK KEDVSESSSA ELDSDGPGSP 500
    AKAKASLALP QKVRPVATQV KTDRGKGHSG SSEESSDSEE EAAPAASAAQ 550
    AKPALEKQMK ASSRKGTPAS ATGASTSSHC KAGAVTSSAS LSSPALAKGT 600
    QRSDVDSSSE SESEGAAPST PRVQGKSGGK GLQGKAALGQ GVAPVHTQKT 650
    GPSVKAMAQE DSESLEEDSS SEEEDETPAQ ATPLGRLPQA KANPPPTKTP 700
    PASASGKAVA APTKGKPPVP NSTVSARGQR SVPAAGKAGA PATQAQKGPV 750
    AGTGEDSESS SKEESDSEEE TPAQIKPVGK TSQVRAASAP AKESPKKGAH 800
    PGTPGKTGSS ATQAQPGKTE DSDSSSEESD SDTEMPSAQA IKSPPVSVNR 850
    NSSPAVPAPT PEGVQAVNTT KKASGTTAQS SSSESEDGDE DLIPATQPST 900
    YALRTSVTTP AALSRAASQP SKSEQSSRMP KGKKAKAAAS AQTSSAVETL 950
    PMMPPQSAPI QPKATNKLGK SKLPEKQQLA PGYPKAPRSS EDSSDTSSED 1000
    EEDAKRPQMP KSAHRLDPDP SQKETVVEET PTESSEDEMV APSQSLLSGY 1050
    MTPGLTVANS QASKATPRPD SNSLASSAPA TKDNPDGKQK SKSQHAADTA 1100
    LPKTGRKEAS SGSTPQKPKK LKKSTSSSPA PTQTLPNSIT QRLLEQAWPL 1150
    SEAQVQASVV KVLTELLEQE RLKATEAIKE SGKKSQKRKL SGDLEAGAPK 1200
    NKKKKEQPVP RASAVSPEKA PMTSKAKSKL DKGSAGGKGK GSPGPQGAKE 1250
    KPDGELLGIK LESGEQSDPK SKSKKKKSLK KKKDKEKKEK KKGKKSLAKD 1300
    SASPIQKKKK KKKKSAEPAV 1320
    Length:1,320
    Mass (Da):135,001
    Last modified:July 1, 1997 - v1
    Checksum:i34D87F5F5D300758
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti35 – 351G → A in AAB60933. (PubMed:9158147)Curated
    Sequence conflicti125 – 14218Missing in AAB60933. (PubMed:9158147)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF001794 mRNA. Translation: AAB71347.1.
    U81030 mRNA. Translation: AAB60933.1.
    BC060105 mRNA. Translation: AAH60105.1.
    CCDSiCCDS37836.1.
    PIRiJC5630.
    RefSeqiNP_035682.1. NM_011552.3.
    UniGeneiMm.486251.

    Genome annotation databases

    EnsembliENSMUST00000176630; ENSMUSP00000135476; ENSMUSG00000024613.
    GeneIDi21453.
    KEGGimmu:21453.
    UCSCiuc008fbb.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF001794 mRNA. Translation: AAB71347.1 .
    U81030 mRNA. Translation: AAB60933.1 .
    BC060105 mRNA. Translation: AAH60105.1 .
    CCDSi CCDS37836.1.
    PIRi JC5630.
    RefSeqi NP_035682.1. NM_011552.3.
    UniGenei Mm.486251.

    3D structure databases

    ProteinModelPortali O08784.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204043. 1 interaction.
    IntActi O08784. 2 interactions.
    MINTi MINT-4137233.

    PTM databases

    PhosphoSitei O08784.

    Proteomic databases

    MaxQBi O08784.
    PaxDbi O08784.
    PRIDEi O08784.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000176630 ; ENSMUSP00000135476 ; ENSMUSG00000024613 .
    GeneIDi 21453.
    KEGGi mmu:21453.
    UCSCi uc008fbb.2. mouse.

    Organism-specific databases

    CTDi 6949.
    MGIi MGI:892003. Tcof1.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00730000111382.
    HOGENOMi HOG000154524.
    HOVERGENi HBG006664.
    InParanoidi O08784.
    KOi K14562.
    PhylomeDBi O08784.
    TreeFami TF341730.

    Miscellaneous databases

    NextBioi 300816.
    PMAP-CutDB O08784.
    PROi O08784.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O08784.
    Bgeei O08784.
    Genevestigatori O08784.

    Family and domain databases

    InterProi IPR006594. LisH_dimerisation.
    IPR003993. TCS_treacle.
    IPR017859. Treacle-like_TCS.
    [Graphical view ]
    Pfami PF03546. Treacle. 2 hits.
    [Graphical view ]
    PRINTSi PR01503. TREACLE.
    SMARTi SM00667. LisH. 1 hit.
    [Graphical view ]
    PROSITEi PS50896. LISH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse TCOF1 is expressed widely, has motifs conserved in nucleolar phosphoproteins, and maps to chromosome 18."
      Paznekas W.A., Zhang N., Gridley T., Jabs E.W.
      Biochem. Biophys. Res. Commun. 238:1-6(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Sequence analysis, identification of evolutionary conserved motifs and expression analysis of murine tcof1 provide further evidence for a potential function for the gene and its human homologue, TCOF1."
      Dixon J., Hovanes K., Shiang R., Dixon M.J.
      Hum. Mol. Genet. 6:727-737(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1314.
      Strain: C57BL/6.
      Tissue: Brain.
    4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169 AND THR-171, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169; THR-171; SER-593 AND SER-1216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-132; LYS-318; LYS-327; LYS-626; LYS-630; LYS-922 AND LYS-976, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiTCOF_MOUSE
    AccessioniPrimary (citable) accession number: O08784
    Secondary accession number(s): O08857
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 2, 2004
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3