Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O08782

- ALD2_CRIGR

UniProt

O08782 - ALD2_CRIGR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Aldose reductase-related protein 2

Gene

AKR1B8

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Reductase with a preference for aliphatic substrates. Can also act on small aromatic aldehydes, steroid aldehydes and some ketone substrates.1 Publication

Catalytic activityi

Alditol + NAD(P)+ = aldose + NAD(P)H.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei49 – 491Proton donorBy similarity
Sitei78 – 781Lowers pKa of active site TyrBy similarity
Binding sitei111 – 1111SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi211 – 27363NADP1 PublicationAdd
BLAST

GO - Molecular functioni

  1. alditol:NADP+ 1-oxidoreductase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Aldose reductase-related protein 2 (EC:1.1.1.21)
Short name:
AR
Alternative name(s):
Aldehyde reductase
Aldo-keto reductase
Gene namesi
Name:AKR1B8
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Aldose reductase-related protein 2PRO_0000124630Add
BLAST

Expressioni

Tissue specificityi

Detected at very low levels in urinary bladder, testis and jejunum.1 Publication

Inductioni

By FGF-1 (By similarity). Up-regulated by calpain inhibitor I (N-acetyl-leucyl-leucyl-norleucinal/ALLN).By similarity1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

Secondary structure

1
316
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 63Combined sources
Beta strandi12 – 165Combined sources
Helixi25 – 3814Combined sources
Beta strandi42 – 443Combined sources
Helixi47 – 493Combined sources
Helixi52 – 6413Combined sources
Helixi70 – 723Combined sources
Beta strandi74 – 796Combined sources
Helixi81 – 833Combined sources
Helixi86 – 10015Combined sources
Beta strandi105 – 1117Combined sources
Helixi138 – 15013Combined sources
Beta strandi153 – 1553Combined sources
Beta strandi157 – 1615Combined sources
Helixi164 – 1718Combined sources
Beta strandi182 – 1865Combined sources
Helixi194 – 2029Combined sources
Beta strandi206 – 2105Combined sources
Beta strandi218 – 2203Combined sources
Turni228 – 2303Combined sources
Helixi232 – 2409Combined sources
Helixi245 – 25410Combined sources
Turni255 – 2573Combined sources
Helixi267 – 2737Combined sources
Helixi283 – 2908Combined sources
Helixi302 – 3043Combined sources
Beta strandi305 – 3073Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C9WX-ray2.40A2-316[»]
ProteinModelPortaliO08782.
SMRiO08782. Positions 2-315.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08782.

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

HOVERGENiHBG000020.
KOiK00011.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08782-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTFVELSTK AKMPIVGLGT WQSPPGQVKE AVKVAIDAGY RHIDCAYAYY
60 70 80 90 100
NEHEVGEAIQ EKIKEKAVRR EDLFIVSKLW PTCFERKLLK EAFQKTLTDL
110 120 130 140 150
KLDYLDLYLI HWPQGLQPGK ELFPKDDQGN VLTSKITFLD AWEVMEELVD
160 170 180 190 200
EGLVKALGVS NFNHFQIERI LNKPGLKHKP VTNQVECHPY LTQEKLIEYC
210 220 230 240 250
HSKGITVTAY SPLGSPNRPW AKPEDPSLLE DPKIKEIAAK HKKTSAQVLI
260 270 280 290 300
RFHIQRNVVV IPKSVTPARI HENFQVFDFQ LSDQEMATIL GFNRNWRACL
310
LPETVNMEEY PYDAEY
Length:316
Mass (Da):36,340
Last modified:January 23, 2007 - v3
Checksum:i80B4B8BF0F4FDDAE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U81045 mRNA. Translation: AAC53199.1.
RefSeqiNP_001233680.1. NM_001246751.1.

Genome annotation databases

GeneIDi100689318.
KEGGicge:100689318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U81045 mRNA. Translation: AAC53199.1 .
RefSeqi NP_001233680.1. NM_001246751.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1C9W X-ray 2.40 A 2-316 [» ]
ProteinModelPortali O08782.
SMRi O08782. Positions 2-315.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100689318.
KEGGi cge:100689318.

Organism-specific databases

CTDi 14187.

Phylogenomic databases

HOVERGENi HBG000020.
KOi K00011.

Miscellaneous databases

EvolutionaryTracei O08782.

Family and domain databases

Gene3Di 3.20.20.100. 1 hit.
InterProi IPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view ]
PANTHERi PTHR11732. PTHR11732. 1 hit.
Pfami PF00248. Aldo_ket_red. 1 hit.
[Graphical view ]
PIRSFi PIRSF000097. AKR. 1 hit.
PRINTSi PR00069. ALDKETRDTASE.
SUPFAMi SSF51430. SSF51430. 1 hit.
PROSITEi PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, sequencing, and enzymatic activity of an inducible aldo-keto reductase from Chinese hamster ovary cells."
    Hyndman D.J., Takenoshita R., Vera N.L., Pang S.C., Flynn T.G.
    J. Biol. Chem. 272:13286-13291(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY.
    Tissue: Ovary.
  2. "Crystal structure of CHO reductase, a member of the aldo-keto reductase superfamily."
    Ye Q., Hyndman D.J., Li X., Flynn T.G., Jia Z.
    Proteins 38:41-48(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH NADPH.

Entry informationi

Entry nameiALD2_CRIGR
AccessioniPrimary (citable) accession number: O08782
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3