Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O08782

- ALD2_CRIGR

UniProt

O08782 - ALD2_CRIGR

Protein

Aldose reductase-related protein 2

Gene

AKR1B8

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Reductase with a preference for aliphatic substrates. Can also act on small aromatic aldehydes, steroid aldehydes and some ketone substrates.1 Publication

    Catalytic activityi

    Alditol + NAD(P)+ = aldose + NAD(P)H.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei49 – 491Proton donorBy similarity
    Sitei78 – 781Lowers pKa of active site TyrBy similarity
    Binding sitei111 – 1111SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi211 – 27363NADP1 PublicationAdd
    BLAST

    GO - Molecular functioni

    1. alditol:NADP+ 1-oxidoreductase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aldose reductase-related protein 2 (EC:1.1.1.21)
    Short name:
    AR
    Alternative name(s):
    Aldehyde reductase
    Aldo-keto reductase
    Gene namesi
    Name:AKR1B8
    OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
    Taxonomic identifieri10029 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 316316Aldose reductase-related protein 2PRO_0000124630Add
    BLAST

    Expressioni

    Tissue specificityi

    Detected at very low levels in urinary bladder, testis and jejunum.1 Publication

    Inductioni

    By FGF-1 By similarity. Up-regulated by calpain inhibitor I (N-acetyl-leucyl-leucyl-norleucinal/ALLN).By similarity1 Publication

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    Secondary structure

    1
    316
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 63
    Beta strandi12 – 165
    Helixi25 – 3814
    Beta strandi42 – 443
    Helixi47 – 493
    Helixi52 – 6413
    Helixi70 – 723
    Beta strandi74 – 796
    Helixi81 – 833
    Helixi86 – 10015
    Beta strandi105 – 1117
    Helixi138 – 15013
    Beta strandi153 – 1553
    Beta strandi157 – 1615
    Helixi164 – 1718
    Beta strandi182 – 1865
    Helixi194 – 2029
    Beta strandi206 – 2105
    Beta strandi218 – 2203
    Turni228 – 2303
    Helixi232 – 2409
    Helixi245 – 25410
    Turni255 – 2573
    Helixi267 – 2737
    Helixi283 – 2908
    Helixi302 – 3043
    Beta strandi305 – 3073

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1C9WX-ray2.40A2-316[»]
    ProteinModelPortaliO08782.
    SMRiO08782. Positions 2-315.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO08782.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldo/keto reductase family.Curated

    Phylogenomic databases

    HOVERGENiHBG000020.
    KOiK00011.

    Family and domain databases

    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O08782-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTFVELSTK AKMPIVGLGT WQSPPGQVKE AVKVAIDAGY RHIDCAYAYY    50
    NEHEVGEAIQ EKIKEKAVRR EDLFIVSKLW PTCFERKLLK EAFQKTLTDL 100
    KLDYLDLYLI HWPQGLQPGK ELFPKDDQGN VLTSKITFLD AWEVMEELVD 150
    EGLVKALGVS NFNHFQIERI LNKPGLKHKP VTNQVECHPY LTQEKLIEYC 200
    HSKGITVTAY SPLGSPNRPW AKPEDPSLLE DPKIKEIAAK HKKTSAQVLI 250
    RFHIQRNVVV IPKSVTPARI HENFQVFDFQ LSDQEMATIL GFNRNWRACL 300
    LPETVNMEEY PYDAEY 316
    Length:316
    Mass (Da):36,340
    Last modified:January 23, 2007 - v3
    Checksum:i80B4B8BF0F4FDDAE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U81045 mRNA. Translation: AAC53199.1.
    RefSeqiNP_001233680.1. NM_001246751.1.

    Genome annotation databases

    GeneIDi100689318.
    KEGGicge:100689318.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U81045 mRNA. Translation: AAC53199.1 .
    RefSeqi NP_001233680.1. NM_001246751.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1C9W X-ray 2.40 A 2-316 [» ]
    ProteinModelPortali O08782.
    SMRi O08782. Positions 2-315.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100689318.
    KEGGi cge:100689318.

    Organism-specific databases

    CTDi 14187.

    Phylogenomic databases

    HOVERGENi HBG000020.
    KOi K00011.

    Miscellaneous databases

    EvolutionaryTracei O08782.

    Family and domain databases

    Gene3Di 3.20.20.100. 1 hit.
    InterProi IPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view ]
    PANTHERi PTHR11732. PTHR11732. 1 hit.
    Pfami PF00248. Aldo_ket_red. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000097. AKR. 1 hit.
    PRINTSi PR00069. ALDKETRDTASE.
    SUPFAMi SSF51430. SSF51430. 1 hit.
    PROSITEi PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing, and enzymatic activity of an inducible aldo-keto reductase from Chinese hamster ovary cells."
      Hyndman D.J., Takenoshita R., Vera N.L., Pang S.C., Flynn T.G.
      J. Biol. Chem. 272:13286-13291(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY.
      Tissue: Ovary.
    2. "Crystal structure of CHO reductase, a member of the aldo-keto reductase superfamily."
      Ye Q., Hyndman D.J., Li X., Flynn T.G., Jia Z.
      Proteins 38:41-48(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH NADPH.

    Entry informationi

    Entry nameiALD2_CRIGR
    AccessioniPrimary (citable) accession number: O08782
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 72 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3