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Protein

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3

Gene

Ndufaf3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Essential factor for the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).By similarity

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-6799198. Complex I biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3
Alternative name(s):
Nuclear protein E3-3
Gene namesi
Name:Ndufaf3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi708545. Ndufaf3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3PRO_0000281158Add
BLAST

Proteomic databases

PaxDbiO08776.
PRIDEiO08776.

Expressioni

Tissue specificityi

Expressed in testis.1 Publication

Gene expression databases

GenevisibleiO08776. RN.

Interactioni

Subunit structurei

Interacts with NDUFAF4, NDUFS2 and NDUFS3.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027262.

Structurei

3D structure databases

ProteinModelPortaliO08776.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiKOG3363. Eukaryota.
ENOG4111J7F. LUCA.
GeneTreeiENSGT00390000018312.
HOGENOMiHOG000261143.
InParanoidiO08776.
KOiK09008.
OMAiSKAYDHD.
OrthoDBiEOG7VHT09.
PhylomeDBiO08776.
TreeFamiTF321072.

Family and domain databases

Gene3Di3.40.1230.10. 1 hit.
InterProiIPR007523. NDUFAF3/AAMDC.
[Graphical view]
PANTHERiPTHR21192. PTHR21192. 1 hit.
PfamiPF04430. DUF498. 1 hit.
[Graphical view]
SUPFAMiSSF64076. SSF64076. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O08776-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATALGFRCL YRTRPATLGR YVDRLWRSPS RGHRLSPADD ELYQRTRISL
60 70 80 90 100
LQNEFPQAVY IDSYNSRGFT INGNRVFGPC ALLPQTVVQW NVGSHQDITE
110 120 130 140 150
ESFSIFWMLE PRIEIVVVGT GNKTERLHSQ VLQAMRQRGI AVEIQDTPNA
160 170 180
CATFNFLCHE GRVTGAALIP PPGETALASS GQTTE
Length:185
Mass (Da):20,696
Last modified:July 1, 1997 - v1
Checksum:iB47AFEF6D0D43C14
GO
Isoform 2 (identifier: O08776-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     93-100: GSHQDITE → SPPLHCNL
     101-185: Missing.

Show »
Length:100
Mass (Da):11,482
Checksum:iB2EE20F8C26406C3
GO
Isoform 3 (identifier: O08776-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     93-109: GSHQDITEESFSIFWML → SPPLHCNLYVVPKATPS
     110-185: Missing.

Show »
Length:109
Mass (Da):12,425
Checksum:iCB239BBB47104E17
GO
Isoform 4 (identifier: O08776-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     21-26: Missing.

Show »
Length:179
Mass (Da):19,863
Checksum:i6332EB42DC52C518
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei21 – 266Missing in isoform 4. 1 PublicationVSP_023992
Alternative sequencei93 – 10917GSHQD…IFWML → SPPLHCNLYVVPKATPS in isoform 3. 1 PublicationVSP_023994Add
BLAST
Alternative sequencei93 – 1008GSHQDITE → SPPLHCNL in isoform 2. 1 PublicationVSP_023993
Alternative sequencei101 – 18585Missing in isoform 2. 1 PublicationVSP_023996Add
BLAST
Alternative sequencei110 – 18576Missing in isoform 3. 1 PublicationVSP_023995Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95160 mRNA. Translation: AAB54063.1.
U95161 mRNA. Translation: AAB54064.1.
U95162 mRNA. Translation: AAB54065.1.
BC079306 mRNA. Translation: AAH79306.1.
RefSeqiNP_001029143.1. NM_001033971.2. [O08776-4]
NP_064465.1. NM_020080.3. [O08776-1]
XP_006243907.1. XM_006243845.2. [O08776-1]
UniGeneiRn.40118.

Genome annotation databases

EnsembliENSRNOT00000027262; ENSRNOP00000027262; ENSRNOG00000020068. [O08776-1]
GeneIDi56769.
KEGGirno:56769.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95160 mRNA. Translation: AAB54063.1.
U95161 mRNA. Translation: AAB54064.1.
U95162 mRNA. Translation: AAB54065.1.
BC079306 mRNA. Translation: AAH79306.1.
RefSeqiNP_001029143.1. NM_001033971.2. [O08776-4]
NP_064465.1. NM_020080.3. [O08776-1]
XP_006243907.1. XM_006243845.2. [O08776-1]
UniGeneiRn.40118.

3D structure databases

ProteinModelPortaliO08776.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027262.

Proteomic databases

PaxDbiO08776.
PRIDEiO08776.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027262; ENSRNOP00000027262; ENSRNOG00000020068. [O08776-1]
GeneIDi56769.
KEGGirno:56769.

Organism-specific databases

CTDi25915.
RGDi708545. Ndufaf3.

Phylogenomic databases

eggNOGiKOG3363. Eukaryota.
ENOG4111J7F. LUCA.
GeneTreeiENSGT00390000018312.
HOGENOMiHOG000261143.
InParanoidiO08776.
KOiK09008.
OMAiSKAYDHD.
OrthoDBiEOG7VHT09.
PhylomeDBiO08776.
TreeFamiTF321072.

Enzyme and pathway databases

ReactomeiR-RNO-6799198. Complex I biogenesis.

Miscellaneous databases

PROiO08776.

Gene expression databases

GenevisibleiO08776. RN.

Family and domain databases

Gene3Di3.40.1230.10. 1 hit.
InterProiIPR007523. NDUFAF3/AAMDC.
[Graphical view]
PANTHERiPTHR21192. PTHR21192. 1 hit.
PfamiPF04430. DUF498. 1 hit.
[Graphical view]
SUPFAMiSSF64076. SSF64076. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a novel alternatively spliced nuclear protein."
    Hartmann A.M., Stamm S.
    Biochim. Biophys. Acta 1353:224-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    Tissue: Testis.

Entry informationi

Entry nameiNDUF3_RAT
AccessioniPrimary (citable) accession number: O08776
Secondary accession number(s): O08777, Q6AXV1, Q78E24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 1, 1997
Last modified: June 8, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.