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Reviewed, UniProtKB/Swiss-Prot O08775 (VGFR2_RAT)

Last modified June 16, 2009. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Vascular endothelial growth factor receptor 2
      Short name=VEGFR-2
    EC=2.7.10.1
Alternative name(s):
    Protein-tyrosine kinase receptor flk-1
    Fetal liver kinase 1
    CD_antigen=CD309
Gene names
Name: Kdr
Synonyms: Flk1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length1343 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability By similarity.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Interacts with SHB; upon VEGF activation. Interacts with MYOF By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed in the post-pubertal mammary glands. Ref.2

Developmental stage

Increases during pregnancy (1.6-fold at 4 days) and lactation (3.8-fold at 7 days). Decreases in the early phases of involution (45%, 50% and 34% on days 1, 2, and 3 respectively). Ref.2

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 protein kinase domain.

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Ontologies

Keywords
   Biological processAngiogenesis
Differentiation
   Cellular componentMembrane
   DomainImmunoglobulin domain
Repeat
Signal
Transmembrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Kinase
Receptor
Transferase
Tyrosine-protein kinase
   PTMGlycoprotein
Phosphoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processangiogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

elevation of cytosolic calcium ion concentration

Inferred from mutant phenotype. Source: RGD

male gonad development

Inferred from direct assay. Source: RGD

neuron projection morphogenesis

Inferred from mutant phenotype. Source: RGD

neuroprotection

Inferred from mutant phenotype. Source: RGD

positive regulation of TOR signaling pathway

Inferred from mutant phenotype. Source: RGD

positive regulation of angiogenesis

Inferred from direct assay. Source: RGD

positive regulation of calcium-mediated signaling

Inferred from mutant phenotype. Source: RGD

positive regulation of cell migration

Inferred from mutant phenotype. Source: RGD

positive regulation of cell proliferation

Inferred from mutant phenotype. Source: RGD

positive regulation of long-term neuronal synaptic plasticity

Inferred from mutant phenotype. Source: RGD

positive regulation of phosphoinositide 3-kinase cascade

Inferred from mutant phenotype. Source: RGD

positive regulation of vasodilation

Inferred from genetic interaction. Source: RGD

protein amino acid autophosphorylation

Inferred from direct assay. Source: RGD

response to hypoxia

Inferred from mutant phenotype. Source: RGD

vascular endothelial growth factor receptor signaling pathway

Inferred from mutant phenotype. Source: RGD

   Cellular componentcell soma

Inferred from direct assay. Source: RGD

cytoplasm

Inferred from direct assay. Source: RGD

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane fraction

Inferred from direct assay. Source: RGD

neuron projection

Inferred from direct assay. Source: RGD

plasma membrane

Inferred from direct assay. Source: RGD

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

growth factor binding

Inferred from mutant phenotype. Source: RGD

vascular endothelial growth factor receptor activity

Inferred from mutant phenotype. Source: RGD

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 13431324Vascular endothelial growth factor receptor 2
PRO_0000016773

Regions

Topological domain20 – 760741Extracellular Potential
Transmembrane761 – 78222 Potential
Topological domain783 – 1343561Cytoplasmic Potential
Domain46 – 10964Ig-like C2-type 1
Domain141 – 20767Ig-like C2-type 2
Domain224 – 32097Ig-like C2-type 3
Domain328 – 41487Ig-like C2-type 4
Domain421 – 540120Ig-like C2-type 5
Domain547 – 654108Ig-like C2-type 6
Domain663 – 74987Ig-like C2-type 7
Domain830 – 1158329Protein kinase
Nucleotide binding836 – 8449ATP By similarity

Sites

Active site10241Proton acceptor By similarity
Binding site8641ATP By similarity
Site11711Interaction with SHB By similarity

Amino acid modifications

Modified residue10501Phosphotyrosine By similarity
Modified residue10551Phosphotyrosine; by autocatalysis By similarity
Glycosylation461N-linked (GlcNAc...) Potential
Glycosylation961N-linked (GlcNAc...) Potential
Glycosylation1431N-linked (GlcNAc...) Potential
Glycosylation1581N-linked (GlcNAc...) Potential
Glycosylation2451N-linked (GlcNAc...) Potential
Glycosylation3181N-linked (GlcNAc...) Potential
Glycosylation3741N-linked (GlcNAc...) Potential
Glycosylation3951N-linked (GlcNAc...) Potential
Glycosylation5071N-linked (GlcNAc...) Potential
Glycosylation5761N-linked (GlcNAc...) Potential
Glycosylation6091N-linked (GlcNAc...) Potential
Glycosylation6151N-linked (GlcNAc...) Potential
Glycosylation6271N-linked (GlcNAc...) Potential
Glycosylation6711N-linked (GlcNAc...) Potential
Glycosylation7001N-linked (GlcNAc...) Potential
Glycosylation7171N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
O08775-1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: AD7E509EB62D3FF4

FASTA1,343150,394
        10         20         30         40         50         60 
MESRALLAVA LWFCVETRAA SVGLPGDSLH PPKLSTQKDI LTILANTTLQ ITCRGQRDLD 

        70         80         90        100        110        120 
WLWPNTPRDS EERVLVTECG DSIFCKTLTV PRVVGNDTGA YKCFYRDTDV SSIVYVYVQD 

       130        140        150        160        170        180 
HRSPFIASVS DEHGIVYITE NKNKTVVIPC RGSISNLNVS LCARYPEKRF VPDGNRISWD 

       190        200        210        220        230        240 
SEKGFTIPSY MISYAGMVFC EAKINDETYQ SIMYIVLVVG YRIYDVVLSP PHEIELSAGE 

       250        260        270        280        290        300 
KLVLNCTART ELNVGLDFSW QFPSSKHQHK KIVNRDVKSL PGTVAKMFLS TLTIDSVTKS 

       310        320        330        340        350        360 
DQGEYTCTAY SGLMTKKNKT FVRVHTKPFI AFGSGMKSLV EATVGSQVRI PVKYLSYPAP 

       370        380        390        400        410        420 
DIKWYRNGRP IESNYTMIVG DELTIMEVSE RDAGNYTVIL TNPISMEKQS HMVSLVVNVP 

       430        440        450        460        470        480 
PQIGEKALIS PMDSYQYGTM QTLTCTVYAN PPLHHIQWYW QLEEACSYRP SQTNPYTCKE 

       490        500        510        520        530        540 
WRHVKDFQGG NKIEVTKNQY ALIEGKNKTV STLVIQAAYV SALYKCEAIN KAGRGERVIS 

       550        560        570        580        590        600 
FHVIRGPEIT VQPATQPTER ESMSLLCTAD RNTFENLTWY KLGSQATSVH MGESLTPVCK 

       610        620        630        640        650        660 
NLDALWKLNG TVFSNSTNDI LIVAFQNASL QDQGNYVCSA QDKKTKKRHC LVKQLVILER 

       670        680        690        700        710        720 
MAPMITGNLE NQTTTIGETI EVVCPTSGNP TPLITWFKDN ETLVEDSGIV LKDGNRNLTI 

       730        740        750        760        770        780 
RRVRKEDGGL YTCQACNVLG CARAETLFII EGVQEKTNLE VIILVGTAVI AMFFWLLLVI 

       790        800        810        820        830        840 
LVRTVKRANE GELKTGYLSI VMDPDELPLD ERCERLPYDA SKWEFPRDRL KLGKPLGRGA 

       850        860        870        880        890        900 
FGQVIEADAF GIDKTATCKT VAVKMLKEGA THSEHRALMS ELKILIHIGH HLNVVNLLGA 

       910        920        930        940        950        960 
CTKPGGPLMV IVEFCKFGNL STYLRGKRNE FVPYKSKGAR FRSGKDYVGE LSVDLKRRLD 

       970        980        990       1000       1010       1020 
SITSSQSSAS SGFVEEKSLS DVEEEEASEE LYKDFLTLEH LICYSFQVAK GMEFLASRKC 

      1030       1040       1050       1060       1070       1080 
IHRDLAARNI LLSEKNVVKI CDFGLARDIY KDPDYVRKGD PRLPLKWMAP ETIFDRIYTI 

      1090       1100       1110       1120       1130       1140 
QSGVWSFGVL LWEIFSLGAS PYPGVKIDEK FCRRLKEGTR MRAPDYTTPE MYQTMLDCWH 

      1150       1160       1170       1180       1190       1200 
EDPNQRPAFS ELVEHLGNLL QANAQQDGKD YIVLPMSETL SMEEDSGLSL PTSPVSCMEE 

      1210       1220       1230       1240       1250       1260 
EEVCDPKFHY DNTAGISHYL QNSKRKSRPV SVKTFEDIPL EEPEVKVIPD DSQTDSGMVL 

      1270       1280       1290       1300       1310       1320 
ASEELKTLED RNKLSPSFGG MMPSKSRESV ASEGSNQTSG YQSGYHSDDT DTTVYSSDEA 

      1330       1340 
GLLKLVDVAG HVDSGTTLRS SPV

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References

[1]Wen Y., Edelman J.L., De Vries G.W., Sachs G.
Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Retina.
[2]"Regulation of VEGF and VEGF receptor expression in the rodent mammary gland during pregnancy, lactation, and involution."
Pepper M.S., Baetens D., Mandriota S.J., Di Sanza C., Oikemus S., Lane T.F., Soriano J.V., Montesano R., Iruela-Arispe M.L.
Dev. Dyn. 218:507-524(2000) [PubMed: 10878616] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]Lubec G., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 1040-1047, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.

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Cross-references

Sequence databases

U93306 mRNA. Translation: AAB97508.1.
U93307 mRNA. Translation: AAB97509.1.
IPIIPI00195682.
RefSeqNP_037194.1.
UniGeneRn.88869

3D structure databases

HSSPHSSP built from PDB template 1VR2 based on UniProtKB P35968.
SMRO08775. Positions 811-1167.
ModBaseSearch...

PTM databases

PhosphoSiteO08775.

Genome annotation databases

GeneID25589.
KEGGrno:25589.

Organism-specific databases

RGD2965. Kdr.

Phylogenomic databases

HOVERGENO08775.

Enzyme and pathway databases

BRENDA2.7.10.1. 248.

Family and domain databases

InterProIPR013151. Ig.
IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR001824. Recept_tyr_kinase-III_CS.
IPR001245. Tyr_pkinase.
IPR008266. Tyr_pkinase_AS.
IPR009136. VEGFR2.
IPR009134. VEGFR_N.
[Graphical view]
Gene3DG3DSA:2.60.40.10. Ig-like_fold. 4 hits.
PfamPF07679. I-set. 2 hits.
PF00047. ig. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSPR01832. VEGFRECEPTOR.
PR01834. VEGFRECEPTR2.
ProDomPD000001. Prot_kinase. 2 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00409. IG. 5 hits.
SM00408. IGc2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 5 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio607259.

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Entry information

Entry nameVGFR2_RAT
AccessionPrimary (citable) accession number: O08775
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 1, 1997
Last modified: June 16, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents